JP6393260B2 - 蛍光融合ポリペプチド、該ポリペプチドを含むバイオセンサー及びそれらの使用 - Google Patents

蛍光融合ポリペプチド、該ポリペプチドを含むバイオセンサー及びそれらの使用 Download PDF

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JP6393260B2
JP6393260B2 JP2015519247A JP2015519247A JP6393260B2 JP 6393260 B2 JP6393260 B2 JP 6393260B2 JP 2015519247 A JP2015519247 A JP 2015519247A JP 2015519247 A JP2015519247 A JP 2015519247A JP 6393260 B2 JP6393260 B2 JP 6393260B2
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seq
peptide
fluorescent
fusion polypeptide
messenger
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Japanese (ja)
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JP2015522582A (ja
JP2015522582A5 (enExample
Inventor
サバラ ダネル コルタサル
サバラ ダネル コルタサル
ポゴンサ アイーダ クラリサ サラド
ポゴンサ アイーダ クラリサ サラド
マタ ホルヘ ガミス
マタ ホルヘ ガミス
フェレル メリチェイ ロウラ
フェレル メリチェイ ロウラ
ロペス ローサ メジャ
ロペス ローサ メジャ
ロサノ パトリシア ビジャーチェ
ロサノ パトリシア ビジャーチェ
Original Assignee
イノベーティブ テクノロジーズ イン バイオロジカル システムズ エセ．エレ．
イノベーティブ テクノロジーズ イン バイオロジカル システムズ エセ．エレ．
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5035—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on sub-cellular localization
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C07K14/7155—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • Urology & Nephrology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2015519247A 2012-07-06 2013-07-08 蛍光融合ポリペプチド、該ポリペプチドを含むバイオセンサー及びそれらの使用 Active JP6393260B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12382272 2012-07-06
EP12382272.8 2012-07-06
PCT/EP2013/064400 WO2014006225A1 (en) 2012-07-06 2013-07-08 Fluorescent fusion polypeptide, biosensor comprising said polypeptide and uses thereof

Publications (3)

Publication Number Publication Date
JP2015522582A JP2015522582A (ja) 2015-08-06
JP2015522582A5 JP2015522582A5 (enExample) 2016-08-04
JP6393260B2 true JP6393260B2 (ja) 2018-09-19

Family

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JP2015519247A Active JP6393260B2 (ja) 2012-07-06 2013-07-08 蛍光融合ポリペプチド、該ポリペプチドを含むバイオセンサー及びそれらの使用

Country Status (9)

Country Link
US (3) US9784728B2 (enExample)
EP (1) EP2870475B1 (enExample)
JP (1) JP6393260B2 (enExample)
CN (1) CN104781667B (enExample)
CA (1) CA2878126C (enExample)
DK (1) DK2870475T3 (enExample)
ES (1) ES2645431T3 (enExample)
IN (1) IN2014MN02585A (enExample)
WO (1) WO2014006225A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6393260B2 (ja) 2012-07-06 2018-09-19 イノベーティブ テクノロジーズ イン バイオロジカル システムズ エセ．エレ． 蛍光融合ポリペプチド、該ポリペプチドを含むバイオセンサー及びそれらの使用
WO2014108449A1 (en) 2013-01-08 2014-07-17 Atrogi Ab A screening method, a kit, a method of treatment and a compound for use in a method of treatment
ES2952996T3 (es) * 2017-02-06 2023-11-07 Innovative Tech In Biological Systems Sl Polipéptido de fusión fluorescente capaz de detectar la fosforilación de receptores de membrana celular por GRK (cinasas receptoras de proteína G) y/o tirosina cinasas receptoras (RTK)

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH089640B2 (ja) * 1994-05-19 1996-01-31 佑 本庶 ヒト・インターロイキン−２受容体
US6197928B1 (en) 1997-03-14 2001-03-06 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
US7192932B1 (en) * 1997-04-29 2007-03-20 Lauras As Use of immunomodulating agents
JP3471006B2 (ja) * 1998-05-20 2003-11-25 中外製薬株式会社 新規な遺伝子クローニング方法
CA2349720A1 (en) * 1998-11-12 2000-05-18 Beth Israel Deaconess Medical Center Stimulation of angiogenesis via syndecan-4 cytoplasmic domain signaling pathway
US7060793B2 (en) * 1999-05-21 2006-06-13 The Regents Of The University Of California Circularly permuted fluorescent protein indicators
US20070037165A1 (en) * 2000-09-08 2007-02-15 Applera Corporation Polymorphisms in known genes associated with human disease, methods of detection and uses thereof
US7125660B2 (en) * 2000-09-13 2006-10-24 Archemix Corp. Nucleic acid sensor molecules and methods of using same
CA2453528C (en) * 2001-08-01 2011-07-26 Cellomics, Inc. Novel fusion proteins and assays for molecular binding
WO2004022591A1 (de) * 2002-09-06 2004-03-18 Forschungsverbund Berlin E. V. Akap18 delta, eine neue spleissvariante eines proteinkinase a-ankerproteins und verwendung dieser
JP4214206B2 (ja) * 2002-12-10 2009-01-28 独立行政法人理化学研究所 Ｆｒｅｔを利用した蛍光指示薬
US7488583B2 (en) * 2003-09-25 2009-02-10 Odyssey Thera, Inc. Fragment complementation assays for G-protein-coupled receptors and their signaling pathways
GB0411586D0 (en) * 2004-05-24 2004-06-23 Univ The Glasgow Fluorescent protein
EP1774330A4 (en) * 2004-08-02 2008-10-15 Cellumen Inc METHODS FOR DETECTION OF MOLECULAR INTERACTIONS IN CELLS
EP1997885A1 (en) * 2007-05-29 2008-12-03 AXXAM S.p.A. Fluorescent proteins and methods of use thereof
US20110154515A1 (en) * 2007-08-24 2011-06-23 Oliver Griesbeck Genetically encoded calcium sensors comprising the c-terminal lobe of troponin c and a fluorescence tag
JP2009153399A (ja) * 2007-12-25 2009-07-16 National Institute Of Advanced Industrial & Technology 一分子型リアルタイム生物発光イメージングプローブ
EP2376647B1 (en) * 2009-01-12 2014-03-05 Sutro Biopharma, Inc. Mono charging system for selectively introducing non-native amino acids into proteins using an in vitro protein synthesis system
CN102481348A (zh) * 2009-08-31 2012-05-30 Ibc药品公司 双特异性免疫细胞因子停靠-和-加锁(dnl)复合物及其治疗性用途
JP6393260B2 (ja) 2012-07-06 2018-09-19 イノベーティブ テクノロジーズ イン バイオロジカル システムズ エセ．エレ． 蛍光融合ポリペプチド、該ポリペプチドを含むバイオセンサー及びそれらの使用

Also Published As

Publication number Publication date
EP2870475B1 (en) 2017-06-07
IN2014MN02585A (enExample) 2015-07-24
ES2645431T3 (es) 2017-12-05
US9784728B2 (en) 2017-10-10
CA2878126A1 (en) 2014-01-09
US20180003697A1 (en) 2018-01-04
US10883981B2 (en) 2021-01-05
CA2878126C (en) 2022-05-10
US10877025B2 (en) 2020-12-29
US20150185205A1 (en) 2015-07-02
DK2870475T3 (en) 2017-09-18
JP2015522582A (ja) 2015-08-06
CN104781667B (zh) 2017-06-13
CN104781667A (zh) 2015-07-15
US20180003698A1 (en) 2018-01-04
EP2870475A1 (en) 2015-05-13
WO2014006225A1 (en) 2014-01-09

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