CN104781667B - 荧光融合多肽、包含所述多肽的生物感应器及其用途 - Google Patents

荧光融合多肽、包含所述多肽的生物感应器及其用途 Download PDF

Info

Publication number
CN104781667B
CN104781667B CN201380045944.4A CN201380045944A CN104781667B CN 104781667 B CN104781667 B CN 104781667B CN 201380045944 A CN201380045944 A CN 201380045944A CN 104781667 B CN104781667 B CN 104781667B
Authority
CN
China
Prior art keywords
seq
fluorescent
peptide
fusion polypeptide
polypeptide according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201380045944.4A
Other languages
English (en)
Chinese (zh)
Other versions
CN104781667A (zh
Inventor
丹尼尔·科塔萨尔-萨巴拉
艾达-克拉丽莎·萨拉多-波贡扎
乔治·加米斯-马塔
梅瑞特谢尔·罗拉-费勒
罗莎·麦拉-洛佩斯
帕特丽夏·维拉斯-洛扎诺
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
INNOVATIVE TECHNOLOGIES IN BIOLOGICAL SYSTEMS SL
Original Assignee
INNOVATIVE TECHNOLOGIES IN BIOLOGICAL SYSTEMS SL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by INNOVATIVE TECHNOLOGIES IN BIOLOGICAL SYSTEMS SL filed Critical INNOVATIVE TECHNOLOGIES IN BIOLOGICAL SYSTEMS SL
Publication of CN104781667A publication Critical patent/CN104781667A/zh
Application granted granted Critical
Publication of CN104781667B publication Critical patent/CN104781667B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5035—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on sub-cellular localization
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C07K14/7155—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • Urology & Nephrology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201380045944.4A 2012-07-06 2013-07-08 荧光融合多肽、包含所述多肽的生物感应器及其用途 Active CN104781667B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12382272 2012-07-06
EP12382272.8 2012-07-06
PCT/EP2013/064400 WO2014006225A1 (en) 2012-07-06 2013-07-08 Fluorescent fusion polypeptide, biosensor comprising said polypeptide and uses thereof

Publications (2)

Publication Number Publication Date
CN104781667A CN104781667A (zh) 2015-07-15
CN104781667B true CN104781667B (zh) 2017-06-13

Family

ID=48803514

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201380045944.4A Active CN104781667B (zh) 2012-07-06 2013-07-08 荧光融合多肽、包含所述多肽的生物感应器及其用途

Country Status (9)

Country Link
US (3) US9784728B2 (enExample)
EP (1) EP2870475B1 (enExample)
JP (1) JP6393260B2 (enExample)
CN (1) CN104781667B (enExample)
CA (1) CA2878126C (enExample)
DK (1) DK2870475T3 (enExample)
ES (1) ES2645431T3 (enExample)
IN (1) IN2014MN02585A (enExample)
WO (1) WO2014006225A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6393260B2 (ja) 2012-07-06 2018-09-19 イノベーティブ テクノロジーズ イン バイオロジカル システムズ エセ．エレ． 蛍光融合ポリペプチド、該ポリペプチドを含むバイオセンサー及びそれらの使用
WO2014108449A1 (en) 2013-01-08 2014-07-17 Atrogi Ab A screening method, a kit, a method of treatment and a compound for use in a method of treatment
ES2952996T3 (es) * 2017-02-06 2023-11-07 Innovative Tech In Biological Systems Sl Polipéptido de fusión fluorescente capaz de detectar la fosforilación de receptores de membrana celular por GRK (cinasas receptoras de proteína G) y/o tirosina cinasas receptoras (RTK)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6197928B1 (en) * 1997-03-14 2001-03-06 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
EP1571448A1 (en) * 2002-12-10 2005-09-07 Riken Fluorescent indicator using fret

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH089640B2 (ja) * 1994-05-19 1996-01-31 佑 本庶 ヒト・インターロイキン−２受容体
US7192932B1 (en) * 1997-04-29 2007-03-20 Lauras As Use of immunomodulating agents
JP3471006B2 (ja) * 1998-05-20 2003-11-25 中外製薬株式会社 新規な遺伝子クローニング方法
CA2349720A1 (en) * 1998-11-12 2000-05-18 Beth Israel Deaconess Medical Center Stimulation of angiogenesis via syndecan-4 cytoplasmic domain signaling pathway
US7060793B2 (en) * 1999-05-21 2006-06-13 The Regents Of The University Of California Circularly permuted fluorescent protein indicators
US20070037165A1 (en) * 2000-09-08 2007-02-15 Applera Corporation Polymorphisms in known genes associated with human disease, methods of detection and uses thereof
US7125660B2 (en) * 2000-09-13 2006-10-24 Archemix Corp. Nucleic acid sensor molecules and methods of using same
CA2453528C (en) * 2001-08-01 2011-07-26 Cellomics, Inc. Novel fusion proteins and assays for molecular binding
WO2004022591A1 (de) * 2002-09-06 2004-03-18 Forschungsverbund Berlin E. V. Akap18 delta, eine neue spleissvariante eines proteinkinase a-ankerproteins und verwendung dieser
US7488583B2 (en) * 2003-09-25 2009-02-10 Odyssey Thera, Inc. Fragment complementation assays for G-protein-coupled receptors and their signaling pathways
GB0411586D0 (en) * 2004-05-24 2004-06-23 Univ The Glasgow Fluorescent protein
EP1774330A4 (en) * 2004-08-02 2008-10-15 Cellumen Inc METHODS FOR DETECTION OF MOLECULAR INTERACTIONS IN CELLS
EP1997885A1 (en) * 2007-05-29 2008-12-03 AXXAM S.p.A. Fluorescent proteins and methods of use thereof
US20110154515A1 (en) * 2007-08-24 2011-06-23 Oliver Griesbeck Genetically encoded calcium sensors comprising the c-terminal lobe of troponin c and a fluorescence tag
JP2009153399A (ja) * 2007-12-25 2009-07-16 National Institute Of Advanced Industrial & Technology 一分子型リアルタイム生物発光イメージングプローブ
EP2376647B1 (en) * 2009-01-12 2014-03-05 Sutro Biopharma, Inc. Mono charging system for selectively introducing non-native amino acids into proteins using an in vitro protein synthesis system
CN102481348A (zh) * 2009-08-31 2012-05-30 Ibc药品公司 双特异性免疫细胞因子停靠-和-加锁(dnl)复合物及其治疗性用途
JP6393260B2 (ja) 2012-07-06 2018-09-19 イノベーティブ テクノロジーズ イン バイオロジカル システムズ エセ．エレ． 蛍光融合ポリペプチド、該ポリペプチドを含むバイオセンサー及びそれらの使用

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6197928B1 (en) * 1997-03-14 2001-03-06 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
EP1571448A1 (en) * 2002-12-10 2005-09-07 Riken Fluorescent indicator using fret

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
A high signal-to-noise Ca2+ probe composed of a single green fluorescent protein;Junichi Nakai et al;《Nature Biotechnology》;20010228;第19卷;137-141 *
Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin;Atsushi Miyawaki et al;《Nature》;19970828;第388卷;882-887 *
Imaging endoplasmic reticulum calcium with a fluorescent biosensor in transgenic mice;ManamiHara et al;《American Journal of Physiology Cell Physiology》;20041001;第287卷(第4期);C932-C938 *
Monitoring astrocyte calcium microdomains with improved membrane targeted GCaMP reporters;EIJI SHIGETOMI et al;《Neuron Glia Biol.》;20101216;第6卷(第3期);183–191 *

Also Published As

Publication number Publication date
EP2870475B1 (en) 2017-06-07
IN2014MN02585A (enExample) 2015-07-24
ES2645431T3 (es) 2017-12-05
US9784728B2 (en) 2017-10-10
CA2878126A1 (en) 2014-01-09
US20180003697A1 (en) 2018-01-04
US10883981B2 (en) 2021-01-05
CA2878126C (en) 2022-05-10
US10877025B2 (en) 2020-12-29
US20150185205A1 (en) 2015-07-02
DK2870475T3 (en) 2017-09-18
JP2015522582A (ja) 2015-08-06
CN104781667A (zh) 2015-07-15
US20180003698A1 (en) 2018-01-04
JP6393260B2 (ja) 2018-09-19
EP2870475A1 (en) 2015-05-13
WO2014006225A1 (en) 2014-01-09

Similar Documents

Publication Publication Date Title
EP2073013B1 (en) Method of identifying transmembrane protein-interacting compounds
EP3194960B1 (en) Biosensors for monitoring biomolecule localization and trafficking in cells
KR20140099322A (ko) 단백질 간의 상호작용의 판정 방법
Ji et al. Novel signaling of dynorphin at κ-opioid receptor/bradykinin B2 receptor heterodimers
Pal et al. Divergent β-arrestin-dependent signaling events are dependent upon sequences within G-protein-coupled receptor C termini
US20200400567A1 (en) Fusion polypeptide
Polit et al. The Gαi protein subclass selectivity to the dopamine D2 receptor is also decided by their location at the cell membrane
Lotze et al. Time-resolved tracking of separately internalized neuropeptide Y2 receptors by two-color pulse-chase
CN104781667B (zh) 荧光融合多肽、包含所述多肽的生物感应器及其用途
Ngamlertwong et al. Agonist dependency of the second phase access of β-arrestin 2 to the heteromeric µ-V1b receptor
Carrel et al. Axonal targeting of the 5‐HT1B serotonin receptor relies on structure‐specific constitutive activation
CN114702570A (zh) aGPCR拮抗剂
Alvarez-Curto et al. Defining the functional equivalence of wild-type and chemically engineered G protein-coupled receptors
Gottschalk et al. Detecting protein–protein interactions in living cells: development of a bioluminescence resonance energy transfer assay to evaluate the PSD-95/NMDA receptor interaction
Hanna Molecular pharmacological analysis of the human MrgD receptor
Siu Roles of Calmodulin and a N-methyl-D-aspartate Receptor-Pannexin-1 signaling complex in Connexin-36 Plasticity
Polit et al. The Gαi protein subclass selectivity to the dopamine D
Hannan Cell surface mobility of GABAB receptors
US20160123957A1 (en) Visualization of P-TEFb by Fluorescence Complementation
Schmidt The role of compartmentalised cyclic nucleotides in pulmonary endothelial barrier function
Strungs Elucidation of Mechanisms Modulating the Conformation and Function of β-Arrestins by G Protein-Coupled Receptors
JP2007049943A (ja) 細胞内カルシウムイオン指示機能を有するポリペプチド
Calizo Influence of membrane heterogeneity on G-protein coupled receptor signaling
McAdam Role of the synaptotagmin 1-dynamin 1 interaction in secretory vesicle recycling
Hanna Molecular pharmacological analysis of the human MrgD receptor: New insights into signaling

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
EXSB Decision made by sipo to initiate substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant