IN2014MN02585A - - Google Patents

Info

Publication number
IN2014MN02585A
IN2014MN02585A IN2585MUN2014A IN2014MN02585A IN 2014MN02585 A IN2014MN02585 A IN 2014MN02585A IN 2585MUN2014 A IN2585MUN2014 A IN 2585MUN2014A IN 2014MN02585 A IN2014MN02585 A IN 2014MN02585A
Authority
IN
India
Prior art keywords
peptide
fluorescent
linker
fusion polypeptide
protein binding
Prior art date
Application number
Inventor
Zabala Danel Kortazar
Pogonza Aida Clarisa Salado
Mata Jorge Gámiz
Ferrer Meritxell Roura
López Rosa Mella
Lozano Patricia Villacé
Original Assignee
Innovative Technologies In Biolog Systems S L
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Innovative Technologies In Biolog Systems S L filed Critical Innovative Technologies In Biolog Systems S L
Publication of IN2014MN02585A publication Critical patent/IN2014MN02585A/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5035—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on sub-cellular localization
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C07K14/7155—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction

Abstract

The present invention refers to a fluorescent fusion polypeptide capable of changing its localization within the cell from the cell cytoplasmic membrane to the retention vesicles upon an increase in the concentration of second messengers within the cell cytoplasm comprising a membrane localization peptide a second messenger transduction protein binding peptide a reticulum retention signal and a fluorescent peptide wherein: a. the membrane localization peptide is located at the N terminus of the fluorescent fusion polypeptide and is physically bound optionally through a linker to the fluorescent peptide which in turn is physically bound optionally through a linker to the second messenger transduction protein binding peptide; and b. the second messenger transduction protein binding peptide is physically bound optionally through a linker to the reticulum retention signal which in turn is located at the C terminus of the fluorescent fusion polypeptide.
IN2585MUN2014 2012-07-06 2013-07-08 IN2014MN02585A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP12382272 2012-07-06
PCT/EP2013/064400 WO2014006225A1 (en) 2012-07-06 2013-07-08 Fluorescent fusion polypeptide, biosensor comprising said polypeptide and uses thereof

Publications (1)

Publication Number Publication Date
IN2014MN02585A true IN2014MN02585A (en) 2015-07-24

Family

ID=48803514

Family Applications (1)

Application Number Title Priority Date Filing Date
IN2585MUN2014 IN2014MN02585A (en) 2012-07-06 2013-07-08

Country Status (9)

Country Link
US (3) US9784728B2 (en)
EP (1) EP2870475B1 (en)
JP (1) JP6393260B2 (en)
CN (1) CN104781667B (en)
CA (1) CA2878126C (en)
DK (1) DK2870475T3 (en)
ES (1) ES2645431T3 (en)
IN (1) IN2014MN02585A (en)
WO (1) WO2014006225A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9784728B2 (en) 2012-07-06 2017-10-10 Innovative Technologies In Biological Systems S.L. Fluorescent fusion polypeptide
WO2014108449A1 (en) 2013-01-08 2014-07-17 Atrogi Ab A screening method, a kit, a method of treatment and a compound for use in a method of treatment
ES2952996T3 (en) * 2017-02-06 2023-11-07 Innovative Tech In Biological Systems Sl Fluorescent fusion polypeptide capable of detecting phosphorylation of cell membrane receptors by GRK (G protein receptor kinases) and/or receptor tyrosine kinases (RTK)

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH089640B2 (en) * 1994-05-19 1996-01-31 佑 本庶 Human interleukin-2 receptor
US6197928B1 (en) * 1997-03-14 2001-03-06 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
US7192932B1 (en) * 1997-04-29 2007-03-20 Lauras As Use of immunomodulating agents
EP1081224B1 (en) * 1998-05-20 2006-06-07 Chugai Seiyaku Kabushiki Kaisha Novel method for cloning a gene
EP1131081A4 (en) * 1998-11-12 2006-09-06 Beth Israel Hospital Stimulation of angiogenesis via syndecan-4 cytoplasmic domain signaling pathway
US7060793B2 (en) * 1999-05-21 2006-06-13 The Regents Of The University Of California Circularly permuted fluorescent protein indicators
US20070037165A1 (en) * 2000-09-08 2007-02-15 Applera Corporation Polymorphisms in known genes associated with human disease, methods of detection and uses thereof
US7125660B2 (en) * 2000-09-13 2006-10-24 Archemix Corp. Nucleic acid sensor molecules and methods of using same
WO2003012068A2 (en) * 2001-08-01 2003-02-13 Cellomics, Inc. Novel fusion proteins and assays for molecular binding
CA2497254A1 (en) * 2002-09-06 2004-03-18 Forschungsverbund Berlin E. V. Akap18 delta, a novel splicing variant of a protein kinase a anchor protein and the use of the same
JP4214206B2 (en) * 2002-12-10 2009-01-28 独立行政法人理化学研究所 Fluorescent indicator using FRET
US7488583B2 (en) * 2003-09-25 2009-02-10 Odyssey Thera, Inc. Fragment complementation assays for G-protein-coupled receptors and their signaling pathways
GB0411586D0 (en) * 2004-05-24 2004-06-23 Univ The Glasgow Fluorescent protein
US20090131270A1 (en) * 2004-08-02 2009-05-21 Cellumen, Inc.A Corporation Methods for the detection of molecular interactions within cells
EP1997885A1 (en) * 2007-05-29 2008-12-03 AXXAM S.p.A. Fluorescent proteins and methods of use thereof
US20110154515A1 (en) * 2007-08-24 2011-06-23 Oliver Griesbeck Genetically encoded calcium sensors comprising the c-terminal lobe of troponin c and a fluorescence tag
JP2009153399A (en) * 2007-12-25 2009-07-16 National Institute Of Advanced Industrial & Technology Single molecule-format real-time bioluminescence imaging probe
EP2376647B1 (en) * 2009-01-12 2014-03-05 Sutro Biopharma, Inc. Mono charging system for selectively introducing non-native amino acids into proteins using an in vitro protein synthesis system
CN102481348A (en) * 2009-08-31 2012-05-30 Ibc药品公司 Bispecific immunocytokine dock-and-lock (DNL) complexes and therapeutic use thereof
US9784728B2 (en) 2012-07-06 2017-10-10 Innovative Technologies In Biological Systems S.L. Fluorescent fusion polypeptide

Also Published As

Publication number Publication date
DK2870475T3 (en) 2017-09-18
CA2878126A1 (en) 2014-01-09
EP2870475B1 (en) 2017-06-07
JP6393260B2 (en) 2018-09-19
CN104781667A (en) 2015-07-15
US20180003698A1 (en) 2018-01-04
JP2015522582A (en) 2015-08-06
EP2870475A1 (en) 2015-05-13
ES2645431T3 (en) 2017-12-05
US20180003697A1 (en) 2018-01-04
CN104781667B (en) 2017-06-13
US9784728B2 (en) 2017-10-10
WO2014006225A1 (en) 2014-01-09
CA2878126C (en) 2022-05-10
US20150185205A1 (en) 2015-07-02
US10877025B2 (en) 2020-12-29
US10883981B2 (en) 2021-01-05

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