JP6389473B2 - 高解像能融解の較正の改善 - Google Patents

高解像能融解の較正の改善 Download PDF

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Publication number
JP6389473B2
JP6389473B2 JP2015552042A JP2015552042A JP6389473B2 JP 6389473 B2 JP6389473 B2 JP 6389473B2 JP 2015552042 A JP2015552042 A JP 2015552042A JP 2015552042 A JP2015552042 A JP 2015552042A JP 6389473 B2 JP6389473 B2 JP 6389473B2
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Japan
Prior art keywords
double
stranded oligonucleotide
strand
nucleic acid
chromophore
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Expired - Fee Related
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JP2015552042A
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English (en)
Japanese (ja)
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JP2016504041A (ja
JP2016504041A5 (https=
Inventor
ライザー，アストリト
ザグナー，グレゴール
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2015552042A 2013-01-10 2014-01-08 高解像能融解の較正の改善 Expired - Fee Related JP6389473B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP13150790 2013-01-10
EP13150790.7 2013-01-10
PCT/EP2014/050243 WO2014108446A1 (en) 2013-01-10 2014-01-08 Improved calibration of high resolution melting

Publications (3)

Publication Number Publication Date
JP2016504041A JP2016504041A (ja) 2016-02-12
JP2016504041A5 JP2016504041A5 (https=) 2016-09-23
JP6389473B2 true JP6389473B2 (ja) 2018-09-12

Family

ID=47561350

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2015552042A Expired - Fee Related JP6389473B2 (ja) 2013-01-10 2014-01-08 高解像能融解の較正の改善

Country Status (6)

Country Link
US (1) US20150315634A1 (https=)
EP (1) EP2943587A1 (https=)
JP (1) JP6389473B2 (https=)
CN (1) CN104838017B (https=)
CA (1) CA2896616C (https=)
WO (1) WO2014108446A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2654571C2 (ru) * 2016-02-25 2018-05-21 Общество с ограниченной ответственностью "Научно-производственная фирма ДНК-Технология" (ООО "НПФ ДНК-Технология") Способ оптической и температурной валидации приборов для ПЦР-исследований в режиме реального времени

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
ATE88761T1 (de) * 1986-01-10 1993-05-15 Amoco Corp Kompetitiver homogener test.
US5935791A (en) * 1997-09-23 1999-08-10 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
US20040022764A1 (en) * 2002-07-31 2004-02-05 Hanan Polansky Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease
CA2501144C (en) 2002-10-23 2015-10-06 University Of Utah Research Foundation Amplicon melting analysis with saturation dyes
US7785786B2 (en) * 2006-01-23 2010-08-31 Quest Diagnostics Investments Incorporated Methods for detecting nucleic acids using multiple signals
FR2906532B1 (fr) * 2006-09-28 2008-12-12 Biomerieux Sa Nouvel oligonucleotide marque
EP2116614A1 (en) * 2008-05-06 2009-11-11 Qiagen GmbH Simultaneous detection of multiple nucleic acid sequences in a reaction
US9542526B2 (en) * 2009-03-10 2017-01-10 Canon U.S. Life Sciences, Inc. Method and system for temperature correction in thermal melt analysis
CA2836907C (en) * 2011-06-06 2020-07-21 Waters Technologies Corporation Compositions, methods, and kits for quantifying target analytes in a sample
US20130157376A1 (en) * 2011-12-20 2013-06-20 Idaho Technology, Inc. Thermal Cycler Calibration Device and Related Methods
EP2722399A1 (en) * 2012-10-18 2014-04-23 Roche Diagniostics GmbH Method for preventing high molecular weight products during amplification

Also Published As

Publication number Publication date
EP2943587A1 (en) 2015-11-18
JP2016504041A (ja) 2016-02-12
WO2014108446A1 (en) 2014-07-17
US20150315634A1 (en) 2015-11-05
CA2896616C (en) 2018-03-13
CN104838017A (zh) 2015-08-12
CA2896616A1 (en) 2014-07-17
CN104838017B (zh) 2017-07-21

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