CA2896616C - Improved calibration of high resolution melting - Google Patents

Improved calibration of high resolution melting Download PDF

Info

Publication number
CA2896616C
CA2896616C CA2896616A CA2896616A CA2896616C CA 2896616 C CA2896616 C CA 2896616C CA 2896616 A CA2896616 A CA 2896616A CA 2896616 A CA2896616 A CA 2896616A CA 2896616 C CA2896616 C CA 2896616C
Authority
CA
Canada
Prior art keywords
double stranded
stranded oligonucleotide
strand
nucleic acid
chromophore
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2896616A
Other languages
English (en)
French (fr)
Other versions
CA2896616A1 (en
Inventor
Astrid Reiser
Gregor Sagner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of CA2896616A1 publication Critical patent/CA2896616A1/en
Application granted granted Critical
Publication of CA2896616C publication Critical patent/CA2896616C/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA2896616A 2013-01-10 2014-01-08 Improved calibration of high resolution melting Expired - Fee Related CA2896616C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP13150790 2013-01-10
EP13150790.7 2013-01-10
PCT/EP2014/050243 WO2014108446A1 (en) 2013-01-10 2014-01-08 Improved calibration of high resolution melting

Publications (2)

Publication Number Publication Date
CA2896616A1 CA2896616A1 (en) 2014-07-17
CA2896616C true CA2896616C (en) 2018-03-13

Family

ID=47561350

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2896616A Expired - Fee Related CA2896616C (en) 2013-01-10 2014-01-08 Improved calibration of high resolution melting

Country Status (6)

Country Link
US (1) US20150315634A1 (https=)
EP (1) EP2943587A1 (https=)
JP (1) JP6389473B2 (https=)
CN (1) CN104838017B (https=)
CA (1) CA2896616C (https=)
WO (1) WO2014108446A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2654571C2 (ru) * 2016-02-25 2018-05-21 Общество с ограниченной ответственностью "Научно-производственная фирма ДНК-Технология" (ООО "НПФ ДНК-Технология") Способ оптической и температурной валидации приборов для ПЦР-исследований в режиме реального времени

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
ATE88761T1 (de) * 1986-01-10 1993-05-15 Amoco Corp Kompetitiver homogener test.
US5935791A (en) * 1997-09-23 1999-08-10 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
US20040022764A1 (en) * 2002-07-31 2004-02-05 Hanan Polansky Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease
CA2501144C (en) 2002-10-23 2015-10-06 University Of Utah Research Foundation Amplicon melting analysis with saturation dyes
US7785786B2 (en) * 2006-01-23 2010-08-31 Quest Diagnostics Investments Incorporated Methods for detecting nucleic acids using multiple signals
FR2906532B1 (fr) * 2006-09-28 2008-12-12 Biomerieux Sa Nouvel oligonucleotide marque
EP2116614A1 (en) * 2008-05-06 2009-11-11 Qiagen GmbH Simultaneous detection of multiple nucleic acid sequences in a reaction
US9542526B2 (en) * 2009-03-10 2017-01-10 Canon U.S. Life Sciences, Inc. Method and system for temperature correction in thermal melt analysis
CA2836907C (en) * 2011-06-06 2020-07-21 Waters Technologies Corporation Compositions, methods, and kits for quantifying target analytes in a sample
US20130157376A1 (en) * 2011-12-20 2013-06-20 Idaho Technology, Inc. Thermal Cycler Calibration Device and Related Methods
EP2722399A1 (en) * 2012-10-18 2014-04-23 Roche Diagniostics GmbH Method for preventing high molecular weight products during amplification

Also Published As

Publication number Publication date
EP2943587A1 (en) 2015-11-18
JP2016504041A (ja) 2016-02-12
WO2014108446A1 (en) 2014-07-17
US20150315634A1 (en) 2015-11-05
JP6389473B2 (ja) 2018-09-12
CN104838017A (zh) 2015-08-12
CA2896616A1 (en) 2014-07-17
CN104838017B (zh) 2017-07-21

Similar Documents

Publication Publication Date Title
KR102246600B1 (ko) 핵산 검정에서 개선된 용융 판별 및 멀티플렉싱을 위한 프로브
JP5860484B2 (ja) 核酸増幅のためのプライマー、プローブおよび方法
US6506568B2 (en) Method of analyzing single nucleotide polymorphisms using melting curve and restriction endonuclease digestion
US11261481B2 (en) Probes for improved melt discrimination and multiplexing in nucleic acid assays
CN116064748A (zh) 用于变体检测的方法
KR101038137B1 (ko) 서열 차이를 감지하는 방법
US20200172958A1 (en) Multiplex probes
KR20040024556A (ko) 검출 시스템
CN101285095B (zh) 一种检测脱氧核糖核酸的单核苷酸多态性的方法
US8758997B2 (en) Method for detecting polymorphism at nucleotide position-1639 of VKORC1 gene, and nucleic acid probe and kit therefor
CA2896616C (en) Improved calibration of high resolution melting
US20160024563A1 (en) Method for performing a melting curve analysis
JP2005328758A (ja) 核酸増幅方法及びこれを利用した一塩基多型の解析
US20120282610A1 (en) Oligonucleotides for Detection Test of Polymorphism of EGFR Exon 19 and Use Thereof
US12209272B2 (en) Multiple analysis method for amplicon by using fluorescence-based multiple melting analysis
EP4599081A1 (en) Detection of molecular analytes based on tailored probe competition
HK1227444A1 (en) Method for the detection of target nucleic acid sequences
HK1227444B (en) Method for the detection of target nucleic acid sequences

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20150611

MKLA Lapsed

Effective date: 20210831

MKLA Lapsed

Effective date: 20200108