JP6336080B2 - 鋳型切り替え反応を用いるｃＤＮＡ合成および単一細胞トランスクリプトームプロファイリングのための方法および組成物 - Google Patents

鋳型切り替え反応を用いるｃＤＮＡ合成および単一細胞トランスクリプトームプロファイリングのための方法および組成物 Download PDF

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JP6336080B2
JP6336080B2 JP2016536474A JP2016536474A JP6336080B2 JP 6336080 B2 JP6336080 B2 JP 6336080B2 JP 2016536474 A JP2016536474 A JP 2016536474A JP 2016536474 A JP2016536474 A JP 2016536474A JP 6336080 B2 JP6336080 B2 JP 6336080B2
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cdna
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template switching
rna
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サンドベリ リカルド
サンドベリ リカルド
ピセリ シモネ
ピセリ シモネ
アル． ファリダニ ウミッド
アル． ファリダニ ウミッド
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JP2016536474A 2013-08-23 2014-08-22 鋳型切り替え反応を用いるｃＤＮＡ合成および単一細胞トランスクリプトームプロファイリングのための方法および組成物 Active JP6336080B2 (ja)

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US201361869220P 2013-08-23 2013-08-23
US61/869,220 2013-08-23
PCT/US2014/052233 WO2015027135A1 (en) 2013-08-23 2014-08-22 Methods and compositions for cdna synthesis and single-cell transcriptome profiling using template switching reaction

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JP2017253460A Division JP6603699B2 (ja) 2013-08-23 2017-12-28 鋳型切り替え反応を用いるｃＤＮＡ合成および単一細胞トランスクリプトームプロファイリングのための方法および組成物

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JP2017253460A Active JP6603699B2 (ja) 2013-08-23 2017-12-28 鋳型切り替え反応を用いるｃＤＮＡ合成および単一細胞トランスクリプトームプロファイリングのための方法および組成物

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CA (1) CA2921603C (OSRAM)
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CN105579587A (zh) * 2013-08-23 2016-05-11 惠氏公司 用于使用模板转换反应进行cDNA合成和单细胞转录组概况分析的方法和组合物
PT3089822T (pt) * 2013-12-30 2022-05-06 Atreca Inc Análise de ácidos nucleicos associados com células isoladas usando códigos de barras de ácido nucleico
WO2017048993A1 (en) 2015-09-15 2017-03-23 Takara Bio Usa, Inc. Methods for preparing a next generation sequencing (ngs) library from a ribonucleic acid (rna) sample and compositions for practicing the same
SG11201803646YA (en) 2015-11-04 2018-05-30 Atreca Inc Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells
WO2017214417A1 (en) 2016-06-10 2017-12-14 Takara Bio Usa, Inc. Methods and compositions employing blocked primers
CN106498040B (zh) * 2016-10-12 2018-10-23 浙江大学 一种分子标记微珠及基于该分子标记微珠的高通量单细胞测序方法
EP3538662B1 (en) * 2016-11-10 2021-12-01 Takara Bio USA, Inc. Methods of producing amplified double stranded deoxyribonucleic acids and compositions and kits for use therein
JP7155136B2 (ja) 2016-11-11 2022-10-18 バイオ－ラド ラボラトリーズ，インコーポレイティド 核酸試料をプロセシングする方法
CN106868005B (zh) * 2017-03-17 2020-07-21 中山大学附属肿瘤医院 一种用于高效快速扩增cDNA末端的锚定引物及扩增方法
US11946043B2 (en) 2017-09-11 2024-04-02 Ludwig Institute For Cancer Research Ltd Selective labeling of 5-methylcytosine in circulating cell-free DNA
CN108103055B (zh) * 2018-01-09 2021-05-28 序康医疗科技（苏州）有限公司 一种单细胞rna逆转录与文库构建的方法
WO2019152860A1 (en) 2018-02-02 2019-08-08 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Random amplification methods for extremely low input nucleic acids
EP3837380A4 (en) 2018-08-17 2022-05-11 Cellecta, Inc. MULTIPLEX PREPARATION OF BARCODE GENE-SPECIFIC DNA FRAGMENTS
EP3902922A1 (en) 2018-12-28 2021-11-03 Biobloxx AB Method and kit for preparing complementary dna
CN113811610A (zh) * 2019-05-09 2021-12-17 加利福尼亚太平洋生物科学股份有限公司 用于改进cDNA合成的组合物和方法
CN114096679B (zh) 2019-07-11 2024-04-09 学校法人东京理科大学 使用固相载体的核酸扩增方法
US20230193254A1 (en) * 2020-05-20 2023-06-22 Chan Zuckerberg Biohub, Inc. Total rna profiling of biological samples and single cells
CN117247990A (zh) * 2022-06-17 2023-12-19 复旦大学 基于拓扑捕获的rna样本处理系统
CN120693411A (zh) 2023-03-09 2025-09-23 疫苗基因技术株式会社 扩增互补dna链的方法
CN116497105B (zh) * 2023-06-28 2023-09-29 浙江大学 基于末端转移酶的单细胞转录组测序试剂盒及测序方法
CN119776495A (zh) * 2024-12-31 2025-04-08 北京普译生物科技有限公司 一种cDNA建库中的模板转换方法

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JP2016527919A (ja) 2016-09-15
CN115558661A (zh) 2023-01-03
US20160258016A1 (en) 2016-09-08
CA2921603C (en) 2019-11-05
US10266894B2 (en) 2019-04-23
ES2827307T3 (es) 2021-05-20
DK3036336T3 (da) 2020-11-02
CN105579587A (zh) 2016-05-11
WO2015027135A1 (en) 2015-02-26
CA2921603A1 (en) 2015-02-26
EP3036336A4 (en) 2017-06-28
US20190264284A1 (en) 2019-08-29
JP6603699B2 (ja) 2019-11-06
JP2018088924A (ja) 2018-06-14
EP3036336B1 (en) 2020-09-30
EP3036336A1 (en) 2016-06-29

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