JP6333531B2 - 酵母エキス - Google Patents
酵母エキス Download PDFInfo
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- JP6333531B2 JP6333531B2 JP2013202079A JP2013202079A JP6333531B2 JP 6333531 B2 JP6333531 B2 JP 6333531B2 JP 2013202079 A JP2013202079 A JP 2013202079A JP 2013202079 A JP2013202079 A JP 2013202079A JP 6333531 B2 JP6333531 B2 JP 6333531B2
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- yeast
- yeast extract
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- arginine
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- 239000012138 yeast extract Substances 0.000 title claims description 109
- 229940041514 candida albicans extract Drugs 0.000 title claims description 102
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 103
- 235000011007 phosphoric acid Nutrition 0.000 claims description 69
- 239000004475 Arginine Substances 0.000 claims description 51
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 51
- 239000007787 solid Substances 0.000 claims description 31
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims description 21
- 150000003016 phosphoric acids Chemical class 0.000 claims description 19
- 229910019142 PO4 Inorganic materials 0.000 claims description 14
- 235000021317 phosphate Nutrition 0.000 claims description 14
- 150000003013 phosphoric acid derivatives Chemical class 0.000 claims description 12
- 210000005253 yeast cell Anatomy 0.000 description 60
- 235000013305 food Nutrition 0.000 description 40
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 31
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 31
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 30
- 238000000034 method Methods 0.000 description 29
- 230000000704 physical effect Effects 0.000 description 29
- 239000003795 chemical substances by application Substances 0.000 description 21
- 239000002609 medium Substances 0.000 description 21
- 210000004027 cell Anatomy 0.000 description 14
- 239000000796 flavoring agent Substances 0.000 description 14
- 235000019634 flavors Nutrition 0.000 description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 14
- 239000002253 acid Substances 0.000 description 11
- 238000012258 culturing Methods 0.000 description 11
- 238000004519 manufacturing process Methods 0.000 description 10
- 239000000047 product Substances 0.000 description 10
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- 239000007788 liquid Substances 0.000 description 8
- 150000007524 organic acids Chemical class 0.000 description 8
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 7
- 238000000354 decomposition reaction Methods 0.000 description 7
- 239000000284 extract Substances 0.000 description 7
- 230000000813 microbial effect Effects 0.000 description 7
- 239000011574 phosphorus Substances 0.000 description 7
- 229910052698 phosphorus Inorganic materials 0.000 description 7
- 235000002639 sodium chloride Nutrition 0.000 description 7
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 6
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- 238000011156 evaluation Methods 0.000 description 6
- 235000013372 meat Nutrition 0.000 description 6
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- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
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- 235000021060 food property Nutrition 0.000 description 4
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- 241000251468 Actinopterygii Species 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
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- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
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- 229910052757 nitrogen Inorganic materials 0.000 description 3
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- 238000002360 preparation method Methods 0.000 description 3
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 2
- 102100030356 Arginase-2, mitochondrial Human genes 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 208000035404 Autolysis Diseases 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
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- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
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- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
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- UQDJGEHQDNVPGU-UHFFFAOYSA-N serine phosphoethanolamine Chemical compound [NH3+]CCOP([O-])(=O)OCC([NH3+])C([O-])=O UQDJGEHQDNVPGU-UHFFFAOYSA-N 0.000 description 2
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- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 1
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- 229930195725 Mannitol Natural products 0.000 description 1
- UEZVMMHDMIWARA-UHFFFAOYSA-N Metaphosphoric acid Chemical compound OP(=O)=O UEZVMMHDMIWARA-UHFFFAOYSA-N 0.000 description 1
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- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 1
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Landscapes
- General Preparation And Processing Of Foods (AREA)
- Meat, Egg Or Seafood Products (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Description
従って、風味に影響を及ぼす有機酸などの酸を用いることなしに、十分に食品の物性を改良でき、特に酵母臭などの風味変化を抑制できる酵母エキスは未だ求められているといえる。
(1)アルギニンおよびリン酸類を含んでなる酵母エキスであって、該酵母エキスに含まれるアルギニンおよびリン酸類の含有量が、該酵母エキスの全固形分に対して、それぞれ2質量%以上および10質量%以上であり、かつ該酵母エキスに含まれる該リン酸類中のオルトリン酸の含有率が、40質量%以下である、酵母エキス。
(2)酵母エキスに含まれるアルギニンの含有量が、該酵母エキスの全固形分に対して、50質量%以下である、(1)に記載の酵母エキス。
(3)アルギニンおよびリン酸類を含んでなる酵母菌体であって、酵母菌体に含まれるアルギニンおよびリン酸類の含有量が、該酵母菌体の全固形分に対して、それぞれ0.5質量%以上および3質量%以上であり、かつ該酵母菌体に含まれる該リン酸類中のオルトリン酸の含有率が、30質量%以下である、酵母菌体。
(4)(3)に記載の菌体を用いて製造された、酵母エキス。
(5)(1)、(2)、および(4)のいずれかに記載の酵母エキスを含有する、食品の物性改良剤。
(6)食品が畜肉加工品または水産加工品である、(5)に記載の物性改良剤。
(7)(1)、(2)、および(4)に記載の酵母エキスならびに(5)および(6)に記載の物性改良剤のいずれか一つを食品へ加える工程を含む、物性が改良された食品の製造方法。
(8)(1)、(2)、および(4)に記載の酵母エキスならびに(5)および(6)に記載の物性改良剤のいずれか一つを食品へ加えることを特徴とする、食品の物性改良方法。
アルギニンを高含有する能力を有する酵母である特許文献1(WO2011/074359号公報)に記載の「ARG2株」〔NITE BP−849株:アルギナーゼを欠損し、かつアルギニンアナログに耐性を有するサッカロミセス・セレビシエ(Saccharomyces cerevisiae)に属する酵母菌株〕を、糖蜜を糖源とし、アンモニアを窒素源とする液体培地に植菌し、5Lジャーにて培養した。培養中に、適時、リン測定キット(LCK350、HACH LANGE社製)を用いて、培養液中のリン酸類の含有量を定量し、培養液中のリン酸類が枯渇していることを確認しつつ、菌体が十分量増えるまで培養した後、リン酸類を蓄積させるために無機リン酸を適量添加して、さらに2時間培養して培養を終了した。
A1:(酵母エキス固形分含有量)×(酵母エキス質量)
B1:(酵母菌体固形分含有量)×(酵母菌体質量)
下記表3に記載の材料をポリ袋に充填し、ヒートシールした後、氷冷で1時間保存した。その後フードプロセッサーで1分30秒間混合し、Φ30mm塩化ケーシングチューブに充填し密封してハムを調製した。5℃で一晩保存した後、75℃で60分間加熱し、流水で冷却した。
A2=(全質量)―(肉質量)−(ケーシングチューブの質量)
B2=(全質量)―(ケーシングチューブ質量)
A2、B2の全質量:ケーシングチューブに充填されたままのハムの質量
A2の肉質量:ケーシングチューブから取り出したハムの質量
Claims (2)
- アルギニンおよびリン酸類を含んでなる酵母エキスであって、
該酵母エキスに含まれるアルギニンおよびリン酸類の含有量が、該酵母エキスの全固形分に対して、それぞれ2質量%以上および10質量%以上であり、かつ
該酵母エキスに含まれる該リン酸類中のオルトリン酸の含有率が、40質量%以下である、酵母エキス。 - 酵母エキスに含まれるアルギニンの含有量が、該酵母エキスの全固形分に対して、50質量%以下である、請求項1に記載の酵母エキス。
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