JP6262543B2 - 蛍光顕微鏡による試料の観察方法 - Google Patents

蛍光顕微鏡による試料の観察方法 Download PDF

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JP6262543B2
JP6262543B2 JP2014007377A JP2014007377A JP6262543B2 JP 6262543 B2 JP6262543 B2 JP 6262543B2 JP 2014007377 A JP2014007377 A JP 2014007377A JP 2014007377 A JP2014007377 A JP 2014007377A JP 6262543 B2 JP6262543 B2 JP 6262543B2
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sample
excitation light
carbon
absorbance
fluorescence
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JP2014142639A5 (enExample
JP2014142639A (ja
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ブイス バート
ブイス バート
フランシーナ ファン ドリエル リンダ
フランシーナ ファン ドリエル リンダ
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/082—Condensers for incident illumination only
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes

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  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Optics & Photonics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
JP2014007377A 2013-01-22 2014-01-20 蛍光顕微鏡による試料の観察方法 Active JP6262543B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP13152215.3 2013-01-22
EP13152215.3A EP2757402B1 (en) 2013-01-22 2013-01-22 Method of observing samples with a fluorescent microscope

Publications (3)

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JP2014142639A JP2014142639A (ja) 2014-08-07
JP2014142639A5 JP2014142639A5 (enExample) 2017-11-02
JP6262543B2 true JP6262543B2 (ja) 2018-01-17

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JP2014007377A Active JP6262543B2 (ja) 2013-01-22 2014-01-20 蛍光顕微鏡による試料の観察方法

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US (1) US20140203191A1 (enExample)
EP (1) EP2757402B1 (enExample)
JP (1) JP6262543B2 (enExample)
CN (1) CN103940791A (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2824445B1 (en) 2013-07-08 2016-03-02 Fei Company Charged-particle microscopy combined with raman spectroscopy
SE1351503A1 (sv) 2013-12-16 2015-01-07 Per Fogelstrand System och metod för fluorescensmikroskopi med detektering av ljusemission från multipla fluorokromer
US11101104B2 (en) * 2019-08-30 2021-08-24 Fei Company Multi modal cryo compatible GUID grid
EP4407291A3 (en) * 2019-10-04 2024-11-13 Mitegen, LLC Sample supports and sample cooling systems for cryo-electron microscopy
CN111855567B (zh) * 2019-10-16 2021-07-20 中国科学院物理研究所 一种实现光学智能聚焦的透射电镜系统及方法

Family Cites Families (13)

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DE59805560D1 (de) 1997-01-13 2002-10-24 Daniel Studer Probenhalter für wasserhaltige Proben sowie Verfahren zu deren Verwendung
ATE220465T1 (de) * 1997-10-29 2002-07-15 Calum E Macaulay Gerät und verfahren zur mikroskopie unter verwendung räumlich modulierten lichtes
NL1017669C2 (nl) 2001-03-22 2002-09-24 Univ Maastricht Inrichting voor het vervaardigen van preparaten voor een cryo-elektronenmicroscoop.
US6687035B2 (en) * 2001-06-07 2004-02-03 Leica Microsystems Heildelberg Gmbh Method and apparatus for ROI-scan with high temporal resolution
DE60227394D1 (de) * 2001-10-09 2008-08-14 Univ Ruprecht Karls Heidelberg Fernfeld lichtmikroskopische methode und vorrichtung zur bestimmung von mindestens einem objekt kleiner als die benutzte wellenlänge
NL1023717C2 (nl) 2003-06-20 2004-12-21 Fei Co Preparaatdrager voor het dragen van een met een elektronenbundel te doorstralen preparaat.
JP2005216645A (ja) * 2004-01-29 2005-08-11 Jeol Ltd 透過電子顕微鏡
JP4425098B2 (ja) 2004-09-06 2010-03-03 浜松ホトニクス株式会社 蛍光顕微鏡および蛍光相関分光解析装置
US7355696B2 (en) * 2005-02-01 2008-04-08 Arryx, Inc Method and apparatus for sorting cells
JP2006292421A (ja) * 2005-04-06 2006-10-26 Sharp Corp 蛍光検出装置
EP1863066A1 (en) 2006-05-29 2007-12-05 FEI Company Sample carrier and sample holder
EP1953791A1 (en) * 2007-02-05 2008-08-06 FEI Company Apparatus for observing a sample with a particle beam and an optical microscope
EP1998165A1 (en) * 2007-06-01 2008-12-03 The European Community, represented by the European Commission Method of fluorescence imaging

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Publication number Publication date
US20140203191A1 (en) 2014-07-24
EP2757402A1 (en) 2014-07-23
CN103940791A (zh) 2014-07-23
EP2757402B1 (en) 2016-03-30
JP2014142639A (ja) 2014-08-07

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