JP6228323B2 - 高効率エタノール発酵菌 - Google Patents
高効率エタノール発酵菌 Download PDFInfo
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- C12N1/165—Yeast isolates
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
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- C12N15/102—Mutagenizing nucleic acids
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- C12R2001/84—Pichia
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- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
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Description
Meyerozyma guilliermondiiの親株を稲わら由来の糖液を用い培養を行った。熊谷産の稲わらを、等量の25%アンモニア水に80℃3時間漬け込んだ後、アンモニアを放散させた。処理したバイオマスは、pHを10%NaOHで4に調整後、アクレモニウムセルラーゼ(Meiji Seika ファルマ社製)を添加して、50℃で72時間酵素糖化を行った。作成したスラリーはフィルタープレス法にて固液分離を行い液体を回収した。この液体(以下、清澄液ともいう。)を用いて、変異剤を加えながら19ヶ月馴化培養を行い、発酵性能向上株を選抜した。選抜は一定時間後のエタノール量を基準に行った。発酵性能の高い菌株を独立行政法人製品評価技術基盤機構 特許微生物寄託センターに、寄託番号NITE BP−01964として寄託した。
2.1 エタノール産生能
図1は親株であるN株に対するBP−01964株のエタノール産生量を示す。コーンストーバーを希硫酸処理した糖化液をNaOH水溶液でpHを6に調整して用い、同株の培養液を培地のOD600が2.0となるように添加し、30℃、96時間培養した後の培養液中のエタノール量を示す。糖化溶液のグルコースは、63.2g/L、キシロースは34.5g/Lであった。エタノール測定はGC−FID(ジーエルサイエンス社製:GC390B)を用いた。
バイオエタノール産生を行うときに、スラリー、清澄液、どちらを用いた場合であっても効率よく発酵する菌であることが望ましい。そこで、スラリー、清澄液を用いて発酵収率を比較した。発酵収率は以下の式により計算される。
発酵収率=得られたエタノール量(g/L)/発酵開始時の糖液に含まれていたグルコース+キシロース量(g/L)/0.5114
稲わらと同様に、コーンストーバーもバイオエタノール産生に多用されるバイオマスである。本菌株は稲わらから製造した清澄液を用いて馴化を行い、単離した菌株であるが、コーンストーバーでも同様にバイオエタノールを効率よく産生する。
配列番号1:AAGGCTTGGGAACTTTCTTT
配列番号2:AGCAATTGATGATTAATTTT
配列番号3:ATGACCAATTCTCTTGAACA
配列番号4:AAATTGTGCCGTGTCAAACT
配列番号5:GTTGTAGCGGAGGCTCAATT
配列番号6:TGTATAATTTAAATGTGGGT
配列番号7:ATGTCAATTCCAGAATCCAT
配列番号8:CACCTTGGCTGGAAGTGCTG
ピルビン酸デカルボキシラーゼ
配列番号9:ATGACAGAAATTACTTTGGG
配列番号10:ACAAACAAATGCTGAAAAC
キシロースレダクターゼ(XR)
配列番号11:ATGTCTATTACTTTGAACTC配列番号12:CACAAAAGTTGGAATCTTGT
キシリトールデヒドロゲナーゼ(XDH)
配列番号13:ATGACTCCCAACCCATCTTT配列番号14:CTCGGGACCATCTATAATAA
トランスケトラーゼ(TLK)
配列番号15:ATGACCACCGACGACTACGA配列番号16:AACAGCTAGCAAGTCCTGA
蟻酸デヒドロゲナーゼ(FDH)配列番号17:ATGAGTCCAGCAACAAAAGG
配列番号18:TTTCATCTTGTGTCTTTCAC
Claims (1)
- 五炭糖及び六炭糖から効率的にエタノールを産生する発酵菌であって、
稲わら由来の糖化溶液中で変異を誘発することによって訓化培養を行い、
スラリー及び清澄液中の発酵収率が67.0〜87.5%である特許寄託センターに寄託番号NITE BP-01964として寄託されていることを特徴とする高効率エタノール発酵菌。
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/JP2014/082329 WO2016088272A1 (ja) | 2014-12-05 | 2014-12-05 | 高効率エタノール発酵菌 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPWO2016088272A1 JPWO2016088272A1 (ja) | 2017-06-08 |
JP6228323B2 true JP6228323B2 (ja) | 2017-11-15 |
Family
ID=56091243
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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JP2016562188A Active JP6228323B2 (ja) | 2014-12-05 | 2014-12-05 | 高効率エタノール発酵菌 |
Country Status (6)
Country | Link |
---|---|
US (1) | US10294497B2 (ja) |
EP (1) | EP3228697B1 (ja) |
JP (1) | JP6228323B2 (ja) |
CN (1) | CN107109344B (ja) |
BR (1) | BR112017010642A2 (ja) |
WO (1) | WO2016088272A1 (ja) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109706089B (zh) * | 2019-02-14 | 2021-08-10 | 中国石油化工股份有限公司 | 一种耐受抑制物的木糖发酵菌株及其构建方法和应用 |
CN109852555B (zh) * | 2019-02-21 | 2022-04-26 | 仲恺农业工程学院 | 一种耐酸酵母及其应用 |
CN114540404B (zh) * | 2022-02-28 | 2023-11-07 | 大连理工大学 | 一种乙醇发酵过程中二氧化碳原位固定的方法 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7846712B2 (en) | 2006-06-01 | 2010-12-07 | Alliance For Sustainable Energy, Llc | L-arabinose fermenting yeast |
JP5321320B2 (ja) * | 2009-07-27 | 2013-10-23 | 株式会社豊田中央研究所 | 発酵能力が向上された酵母及びその利用 |
US20120282664A1 (en) | 2009-11-30 | 2012-11-08 | Akihiko Kondo | Process for production of ethanol from biomass |
JP2011167096A (ja) * | 2010-02-17 | 2011-09-01 | Kobe Univ | バイオマスからのエタノールの生産方法 |
JP2011193788A (ja) | 2010-03-19 | 2011-10-06 | Toyota Central R&D Labs Inc | 発酵能力が向上された酵母及びその利用 |
ES2665521T3 (es) * | 2010-04-14 | 2018-04-26 | Lesaffre Et Compagnie | Levadura industrial, capaz de producir etanol a partir de al menos una pentosa |
JP2012170422A (ja) | 2011-02-23 | 2012-09-10 | Kyoto Institute Of Technology | キシローストランスポーター活性を有する新規タンパク質および当該タンパク質をコードするポリヌクレオチド、並びにそれらの利用 |
-
2014
- 2014-12-05 EP EP14907540.0A patent/EP3228697B1/en not_active Not-in-force
- 2014-12-05 WO PCT/JP2014/082329 patent/WO2016088272A1/ja active Application Filing
- 2014-12-05 BR BR112017010642A patent/BR112017010642A2/pt not_active Application Discontinuation
- 2014-12-05 CN CN201480083610.0A patent/CN107109344B/zh not_active Expired - Fee Related
- 2014-12-05 US US15/532,826 patent/US10294497B2/en not_active Expired - Fee Related
- 2014-12-05 JP JP2016562188A patent/JP6228323B2/ja active Active
Also Published As
Publication number | Publication date |
---|---|
BR112017010642A2 (pt) | 2017-12-26 |
EP3228697A1 (en) | 2017-10-11 |
WO2016088272A1 (ja) | 2016-06-09 |
CN107109344B (zh) | 2020-08-25 |
CN107109344A (zh) | 2017-08-29 |
US20180237803A1 (en) | 2018-08-23 |
EP3228697B1 (en) | 2019-06-26 |
EP3228697A4 (en) | 2018-05-23 |
US10294497B2 (en) | 2019-05-21 |
JPWO2016088272A1 (ja) | 2017-06-08 |
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