JP6141327B2 - タンパク質存在量の測定による細胞内結合事象の検出 - Google Patents

タンパク質存在量の測定による細胞内結合事象の検出 Download PDF

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JP6141327B2
JP6141327B2 JP2014555836A JP2014555836A JP6141327B2 JP 6141327 B2 JP6141327 B2 JP 6141327B2 JP 2014555836 A JP2014555836 A JP 2014555836A JP 2014555836 A JP2014555836 A JP 2014555836A JP 6141327 B2 JP6141327 B2 JP 6141327B2
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peptide
binding
protein
cell
compound
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JP2015507200A (ja
JP2015507200A5 (enExample
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ラーブ ウィリアム
ラーブ ウィリアム
エス ウェアマン トーマス
エス ウェアマン トーマス
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ディスカヴァーエックス コーポレイション
ディスカヴァーエックス コーポレイション
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2014555836A 2012-02-06 2013-02-05 タンパク質存在量の測定による細胞内結合事象の検出 Active JP6141327B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261595525P 2012-02-06 2012-02-06
US61/595,525 2012-02-06
PCT/US2013/024789 WO2013119579A1 (en) 2012-02-06 2013-02-05 Detection of intracellular binding events by measuring protein abundance

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JP2015507200A JP2015507200A (ja) 2015-03-05
JP2015507200A5 JP2015507200A5 (enExample) 2015-08-06
JP6141327B2 true JP6141327B2 (ja) 2017-06-07

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JP2014555836A Active JP6141327B2 (ja) 2012-02-06 2013-02-05 タンパク質存在量の測定による細胞内結合事象の検出

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US (1) US8945853B2 (enExample)
EP (2) EP3367096B1 (enExample)
JP (1) JP6141327B2 (enExample)
CA (1) CA2863774C (enExample)
WO (1) WO2013119579A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3183580B1 (en) * 2014-08-21 2025-03-26 Eurofins DiscoverX Corporation Homogenous thermal shift ligand binding assay
GB201814451D0 (en) * 2018-09-05 2018-10-17 Valerie Nicholas Carl Kristoffer Methods
CN113785072A (zh) * 2019-03-28 2021-12-10 欧陆迪斯卡沃爱克斯制品有限责任公司 启动子区域分析方法和用于实施该方法的细胞

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JPH0779717B2 (ja) 1984-10-29 1995-08-30 マイクロジエニツクス コ−ポレ−シヨン タンパク質結合酵素相補性検定に関する方法
CA1335707C (en) * 1989-08-15 1995-05-30 Pyare Khanna Drug screening assay
US5444161A (en) 1989-08-16 1995-08-22 Microgenics Corporation Substrates for β-galactosidase
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AU6045900A (en) 1999-05-25 2000-12-12 Panorama Research, Inc. Interaction-activated proteins
US7220552B1 (en) 1999-11-19 2007-05-22 The Board Of Trustees Of The Leland Stanford Junior University Bifunctional molecules and their use in the disruption of protein-protein interactions
US7589167B2 (en) 2000-02-22 2009-09-15 J. David Gladstone Institutes ZA loops of bromodomains
US20040137480A1 (en) 2001-08-30 2004-07-15 Eglen Richard M. Monitoring intracellular proteins
AU2002332724A1 (en) * 2001-08-30 2003-03-18 Discoverx, Inc. Genetic construct intracellular monitoring system
CA2478395A1 (en) 2002-04-08 2003-10-23 Sunesis Pharmaceuticals, Inc. Methods for identifying allosteric sites
JP2007521021A (ja) 2003-11-06 2007-08-02 ディスカヴァーエックス インコーポレイテッド 細胞膜タンパク質の検定
EP2400030B1 (en) 2004-05-18 2016-01-20 The Board of Trustees of the Leland Stanford Junior University Detection of protein translocation by beta-galactosidase reporter fragment complementation
US7842815B2 (en) 2004-06-17 2010-11-30 Infinity Pharmaceuticals, Inc. Compounds and methods for inhibiting the interaction of BCL proteins with binding partners
JP5303448B2 (ja) 2006-03-13 2013-10-02 ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティ 親和性が減少した酵素の相補性レポーター系を用いた分子相互作用の検出
ES2321359B1 (es) * 2006-06-07 2010-03-03 Centro De Investigacion Cooperativa De Biociencias- Cic Biogune Proteinas de fusion de p53 sin actividad de transcripcion y sus aplicaciones.
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US8101373B2 (en) 2007-10-12 2012-01-24 Discoverx Corporation β-galactosidase donor fragments
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WO2012178079A1 (en) 2011-06-23 2012-12-27 Discoverx Corporation Monitoring protein trafficking using beta-galactosidase reporter fragment complementation

Also Published As

Publication number Publication date
EP2812695A1 (en) 2014-12-17
CA2863774C (en) 2016-08-16
EP3367096B1 (en) 2019-11-27
CA2863774A1 (en) 2013-08-15
WO2013119579A1 (en) 2013-08-15
EP3367096A1 (en) 2018-08-29
JP2015507200A (ja) 2015-03-05
WO2013119579A8 (en) 2014-06-26
EP2812695A4 (en) 2015-12-02
US20130203067A1 (en) 2013-08-08
US8945853B2 (en) 2015-02-03

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