CA2863774C - Detection of intracellular binding events by measuring protein abundance - Google Patents

Detection of intracellular binding events by measuring protein abundance Download PDF

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Publication number
CA2863774C
CA2863774C CA2863774A CA2863774A CA2863774C CA 2863774 C CA2863774 C CA 2863774C CA 2863774 A CA2863774 A CA 2863774A CA 2863774 A CA2863774 A CA 2863774A CA 2863774 C CA2863774 C CA 2863774C
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Canada
Prior art keywords
binding
peptide
protein
cell
compound
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Active
Application number
CA2863774A
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English (en)
French (fr)
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CA2863774A1 (en
Inventor
William Raab
Thomas S. Wehrman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eurofins Discoverx Corp
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DiscoveRx Corp
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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA2863774A 2012-02-06 2013-02-05 Detection of intracellular binding events by measuring protein abundance Active CA2863774C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261595525P 2012-02-06 2012-02-06
US61/595,525 2012-02-06
PCT/US2013/024789 WO2013119579A1 (en) 2012-02-06 2013-02-05 Detection of intracellular binding events by measuring protein abundance

Publications (2)

Publication Number Publication Date
CA2863774A1 CA2863774A1 (en) 2013-08-15
CA2863774C true CA2863774C (en) 2016-08-16

Family

ID=48903215

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2863774A Active CA2863774C (en) 2012-02-06 2013-02-05 Detection of intracellular binding events by measuring protein abundance

Country Status (5)

Country Link
US (1) US8945853B2 (enExample)
EP (2) EP3367096B1 (enExample)
JP (1) JP6141327B2 (enExample)
CA (1) CA2863774C (enExample)
WO (1) WO2013119579A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3183580B1 (en) * 2014-08-21 2025-03-26 Eurofins DiscoverX Corporation Homogenous thermal shift ligand binding assay
GB201814451D0 (en) * 2018-09-05 2018-10-17 Valerie Nicholas Carl Kristoffer Methods
CN113785072A (zh) * 2019-03-28 2021-12-10 欧陆迪斯卡沃爱克斯制品有限责任公司 启动子区域分析方法和用于实施该方法的细胞

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4378428A (en) 1981-03-30 1983-03-29 Baker Instruments Corporation Method for carrying out non-isotopic immunoassays, labeled analytes and kits for use in such assays
US4708929A (en) 1984-10-29 1987-11-24 Microgenics Corporation Methods for protein binding enzyme complementation assays
US5604091A (en) 1984-03-01 1997-02-18 Microgenics Corporation Methods for protein binding enzyme complementation
JPH0779717B2 (ja) 1984-10-29 1995-08-30 マイクロジエニツクス コ−ポレ−シヨン タンパク質結合酵素相補性検定に関する方法
CA1335707C (en) * 1989-08-15 1995-05-30 Pyare Khanna Drug screening assay
US5444161A (en) 1989-08-16 1995-08-22 Microgenics Corporation Substrates for β-galactosidase
US5543264A (en) 1990-06-29 1996-08-06 Associated Universities, Inc. Co-factor activated recombinant adenovirus proteinases
US6828099B2 (en) 1998-02-02 2004-12-07 Odyssey Thera Inc. Protein fragment complementation assay (PCA) for the detection of protein-protein, protein-small molecule and protein nucleic acid interactions based on the E. coli TEM-1 β-Lactamase
AU6045900A (en) 1999-05-25 2000-12-12 Panorama Research, Inc. Interaction-activated proteins
US7220552B1 (en) 1999-11-19 2007-05-22 The Board Of Trustees Of The Leland Stanford Junior University Bifunctional molecules and their use in the disruption of protein-protein interactions
US7589167B2 (en) 2000-02-22 2009-09-15 J. David Gladstone Institutes ZA loops of bromodomains
US20040137480A1 (en) 2001-08-30 2004-07-15 Eglen Richard M. Monitoring intracellular proteins
AU2002332724A1 (en) * 2001-08-30 2003-03-18 Discoverx, Inc. Genetic construct intracellular monitoring system
CA2478395A1 (en) 2002-04-08 2003-10-23 Sunesis Pharmaceuticals, Inc. Methods for identifying allosteric sites
JP2007521021A (ja) 2003-11-06 2007-08-02 ディスカヴァーエックス インコーポレイテッド 細胞膜タンパク質の検定
EP2400030B1 (en) 2004-05-18 2016-01-20 The Board of Trustees of the Leland Stanford Junior University Detection of protein translocation by beta-galactosidase reporter fragment complementation
US7842815B2 (en) 2004-06-17 2010-11-30 Infinity Pharmaceuticals, Inc. Compounds and methods for inhibiting the interaction of BCL proteins with binding partners
JP5303448B2 (ja) 2006-03-13 2013-10-02 ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティ 親和性が減少した酵素の相補性レポーター系を用いた分子相互作用の検出
ES2321359B1 (es) * 2006-06-07 2010-03-03 Centro De Investigacion Cooperativa De Biociencias- Cic Biogune Proteinas de fusion de p53 sin actividad de transcripcion y sus aplicaciones.
EP2099925B1 (en) 2006-12-26 2013-02-13 The Board of Trustees of The Leland Stanford Junior University Detection of sub-cellular compartment localization of a cell-surface receptor to an endosome using a reduced affinity enzyme complementation reporter system
US8101373B2 (en) 2007-10-12 2012-01-24 Discoverx Corporation β-galactosidase donor fragments
US8530636B2 (en) 2008-05-07 2013-09-10 The Board Of Trustees Of The Leland Stanford Junior University Method for regulating protein function in cells in vivo using synthetic small molecules
US20100120063A1 (en) 2008-10-10 2010-05-13 Discoverx Corporation GPCR Arrestin Assays
US8211655B2 (en) 2009-02-12 2012-07-03 Discoverx Corporation Wild-type receptor assays
US8080796B1 (en) 2010-06-30 2011-12-20 Ut-Battelle, Llc Standoff spectroscopy using a conditioned target
EP2487183A1 (en) 2011-01-18 2012-08-15 Helmholtz-Zentrum für Infektionsforschung GmbH Ubiquitin-isopeptide probes
WO2012178079A1 (en) 2011-06-23 2012-12-27 Discoverx Corporation Monitoring protein trafficking using beta-galactosidase reporter fragment complementation

Also Published As

Publication number Publication date
EP2812695A1 (en) 2014-12-17
EP3367096B1 (en) 2019-11-27
CA2863774A1 (en) 2013-08-15
WO2013119579A1 (en) 2013-08-15
EP3367096A1 (en) 2018-08-29
JP6141327B2 (ja) 2017-06-07
JP2015507200A (ja) 2015-03-05
WO2013119579A8 (en) 2014-06-26
EP2812695A4 (en) 2015-12-02
US20130203067A1 (en) 2013-08-08
US8945853B2 (en) 2015-02-03

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