JP6130935B2 - 新規なバクテリオファージ及びこれを含む抗菌組成物 - Google Patents
新規なバクテリオファージ及びこれを含む抗菌組成物 Download PDFInfo
- Publication number
- JP6130935B2 JP6130935B2 JP2015560096A JP2015560096A JP6130935B2 JP 6130935 B2 JP6130935 B2 JP 6130935B2 JP 2015560096 A JP2015560096 A JP 2015560096A JP 2015560096 A JP2015560096 A JP 2015560096A JP 6130935 B2 JP6130935 B2 JP 6130935B2
- Authority
- JP
- Japan
- Prior art keywords
- bacteriophage
- φcj22
- clostridium perfringens
- kccm11364p
- active ingredient
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241001515965 unidentified phage Species 0.000 title claims description 149
- 239000000203 mixture Substances 0.000 title claims description 51
- 230000000844 anti-bacterial effect Effects 0.000 title claims description 11
- 241000193468 Clostridium perfringens Species 0.000 claims description 60
- 208000035473 Communicable disease Diseases 0.000 claims description 26
- 239000004480 active ingredient Substances 0.000 claims description 26
- 239000003651 drinking water Substances 0.000 claims description 25
- 238000000034 method Methods 0.000 claims description 24
- 241001465754 Metazoa Species 0.000 claims description 22
- 239000000654 additive Substances 0.000 claims description 19
- 239000003674 animal food additive Substances 0.000 claims description 17
- 206010051606 Necrotising colitis Diseases 0.000 claims description 15
- 230000000996 additive effect Effects 0.000 claims description 15
- 208000015181 infectious disease Diseases 0.000 claims description 15
- 208000004995 necrotizing enterocolitis Diseases 0.000 claims description 15
- 201000006195 perinatal necrotizing enterocolitis Diseases 0.000 claims description 15
- 239000003242 anti bacterial agent Substances 0.000 claims description 14
- 235000020188 drinking water Nutrition 0.000 claims description 13
- 230000003115 biocidal effect Effects 0.000 claims description 9
- 239000000645 desinfectant Substances 0.000 claims description 7
- 230000002265 prevention Effects 0.000 claims description 7
- 239000012459 cleaning agent Substances 0.000 claims description 6
- 239000000243 solution Substances 0.000 description 36
- 239000006228 supernatant Substances 0.000 description 15
- 229920001817 Agar Polymers 0.000 description 14
- 239000008272 agar Substances 0.000 description 14
- 201000010099 disease Diseases 0.000 description 13
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 13
- 241000894006 Bacteria Species 0.000 description 12
- 108020004414 DNA Proteins 0.000 description 12
- 102000053602 DNA Human genes 0.000 description 12
- 235000012206 bottled water Nutrition 0.000 description 12
- 239000002552 dosage form Substances 0.000 description 12
- 230000000694 effects Effects 0.000 description 11
- 108090000623 proteins and genes Proteins 0.000 description 11
- 238000002474 experimental method Methods 0.000 description 10
- 241000287828 Gallus gallus Species 0.000 description 9
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 9
- 235000013330 chicken meat Nutrition 0.000 description 9
- 238000001035 drying Methods 0.000 description 9
- 244000144972 livestock Species 0.000 description 8
- 102000004169 proteins and genes Human genes 0.000 description 8
- NTTIDCCSYIDANP-UHFFFAOYSA-N BCCP Chemical compound BCCP NTTIDCCSYIDANP-UHFFFAOYSA-N 0.000 description 7
- 101710201279 Biotin carboxyl carrier protein Proteins 0.000 description 7
- 101710180532 Biotin carboxyl carrier protein of acetyl-CoA carboxylase Proteins 0.000 description 7
- 206010012735 Diarrhoea Diseases 0.000 description 7
- 229940088710 antibiotic agent Drugs 0.000 description 7
- 230000009089 cytolysis Effects 0.000 description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 6
- 239000000523 sample Substances 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 5
- 241000282898 Sus scrofa Species 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 5
- 230000001965 increasing effect Effects 0.000 description 5
- 208000024891 symptom Diseases 0.000 description 5
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- 241000282887 Suidae Species 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- 244000144977 poultry Species 0.000 description 4
- 235000013594 poultry meat Nutrition 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 238000003906 pulsed field gel electrophoresis Methods 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 108010010803 Gelatin Proteins 0.000 description 3
- 239000002202 Polyethylene glycol Substances 0.000 description 3
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 239000011230 binding agent Substances 0.000 description 3
- 239000006227 byproduct Substances 0.000 description 3
- 239000002775 capsule Substances 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 238000009313 farming Methods 0.000 description 3
- 239000000706 filtrate Substances 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 239000008273 gelatin Substances 0.000 description 3
- 229920000159 gelatin Polymers 0.000 description 3
- 235000019322 gelatine Nutrition 0.000 description 3
- 235000011852 gelatine desserts Nutrition 0.000 description 3
- 230000002068 genetic effect Effects 0.000 description 3
- 230000002147 killing effect Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 230000002101 lytic effect Effects 0.000 description 3
- 235000013372 meat Nutrition 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 229920001223 polyethylene glycol Polymers 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 239000012488 sample solution Substances 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 3
- 239000003826 tablet Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 208000004232 Enteritis Diseases 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 241000588653 Neisseria Species 0.000 description 2
- 108700026244 Open Reading Frames Proteins 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 102000035195 Peptidases Human genes 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000001154 acute effect Effects 0.000 description 2
- 239000000443 aerosol Substances 0.000 description 2
- 230000000845 anti-microbial effect Effects 0.000 description 2
- 239000007900 aqueous suspension Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 238000010411 cooking Methods 0.000 description 2
- 230000002498 deadly effect Effects 0.000 description 2
- 230000018044 dehydration Effects 0.000 description 2
- 238000006297 dehydration reaction Methods 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 239000003937 drug carrier Substances 0.000 description 2
- 238000000635 electron micrograph Methods 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- MVPICKVDHDWCJQ-UHFFFAOYSA-N ethyl 3-pyrrolidin-1-ylpropanoate Chemical compound CCOC(=O)CCN1CCCC1 MVPICKVDHDWCJQ-UHFFFAOYSA-N 0.000 description 2
- 239000003925 fat Substances 0.000 description 2
- 210000003495 flagella Anatomy 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 238000001802 infusion Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 238000007918 intramuscular administration Methods 0.000 description 2
- 238000007912 intraperitoneal administration Methods 0.000 description 2
- 238000001990 intravenous administration Methods 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000000314 lubricant Substances 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000010445 mica Substances 0.000 description 2
- 229910052618 mica group Inorganic materials 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 230000001338 necrotic effect Effects 0.000 description 2
- 238000007911 parenteral administration Methods 0.000 description 2
- 239000000546 pharmaceutical excipient Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 229920002477 rna polymer Polymers 0.000 description 2
- 210000000813 small intestine Anatomy 0.000 description 2
- 229940045902 sodium stearyl fumarate Drugs 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 238000005199 ultracentrifugation Methods 0.000 description 2
- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 1
- VLEIUWBSEKKKFX-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid Chemical compound OCC(N)(CO)CO.OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O VLEIUWBSEKKKFX-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- WIGIZIANZCJQQY-UHFFFAOYSA-N 4-ethyl-3-methyl-N-[2-[4-[[[(4-methylcyclohexyl)amino]-oxomethyl]sulfamoyl]phenyl]ethyl]-5-oxo-2H-pyrrole-1-carboxamide Chemical compound O=C1C(CC)=C(C)CN1C(=O)NCCC1=CC=C(S(=O)(=O)NC(=O)NC2CCC(C)CC2)C=C1 WIGIZIANZCJQQY-UHFFFAOYSA-N 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 229920000945 Amylopectin Polymers 0.000 description 1
- 208000031295 Animal disease Diseases 0.000 description 1
- 240000006439 Aspergillus oryzae Species 0.000 description 1
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000193403 Clostridium Species 0.000 description 1
- 208000037384 Clostridium Infections Diseases 0.000 description 1
- 208000003322 Coinfection Diseases 0.000 description 1
- 238000011537 Coomassie blue staining Methods 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 229920002261 Corn starch Polymers 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- 206010012741 Diarrhoea haemorrhagic Diseases 0.000 description 1
- 235000019739 Dicalciumphosphate Nutrition 0.000 description 1
- 206010013647 Drowning Diseases 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 108700023372 Glycosyltransferases Proteins 0.000 description 1
- 244000017020 Ipomoea batatas Species 0.000 description 1
- 235000002678 Ipomoea batatas Nutrition 0.000 description 1
- 241000186610 Lactobacillus sp. Species 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 229920002774 Maltodextrin Polymers 0.000 description 1
- 239000005913 Maltodextrin Substances 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 206010067993 Mucosal necrosis Diseases 0.000 description 1
- MSFSPUZXLOGKHJ-UHFFFAOYSA-N Muraminsaeure Natural products OC(=O)C(C)OC1C(N)C(O)OC(CO)C1O MSFSPUZXLOGKHJ-UHFFFAOYSA-N 0.000 description 1
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 1
- 206010048685 Oral infection Diseases 0.000 description 1
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 108010013639 Peptidoglycan Proteins 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 241000702202 Siphoviridae Species 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 1
- 239000007984 Tris EDTA buffer Substances 0.000 description 1
- COQLPRJCUIATTQ-UHFFFAOYSA-N Uranyl acetate Chemical compound O.O.O=[U]=O.CC(O)=O.CC(O)=O COQLPRJCUIATTQ-UHFFFAOYSA-N 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000003708 ampul Substances 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- 239000004599 antimicrobial Substances 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 229940064004 antiseptic throat preparations Drugs 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 239000000022 bacteriostatic agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 208000027503 bloody stool Diseases 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 239000007975 buffered saline Substances 0.000 description 1
- 239000006172 buffering agent Substances 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- CJZGTCYPCWQAJB-UHFFFAOYSA-L calcium stearate Chemical compound [Ca+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O CJZGTCYPCWQAJB-UHFFFAOYSA-L 0.000 description 1
- 235000013539 calcium stearate Nutrition 0.000 description 1
- 239000008116 calcium stearate Substances 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- ARQRPTNYUOLOGH-UHFFFAOYSA-N chcl3 chloroform Chemical compound ClC(Cl)Cl.ClC(Cl)Cl ARQRPTNYUOLOGH-UHFFFAOYSA-N 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 235000008504 concentrate Nutrition 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000008120 corn starch Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- NEFBYIFKOOEVPA-UHFFFAOYSA-K dicalcium phosphate Chemical compound [Ca+2].[Ca+2].[O-]P([O-])([O-])=O NEFBYIFKOOEVPA-UHFFFAOYSA-K 0.000 description 1
- 229940038472 dicalcium phosphate Drugs 0.000 description 1
- 229910000390 dicalcium phosphate Inorganic materials 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000013681 dietary sucrose Nutrition 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000007884 disintegrant Substances 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000024346 drought recovery Effects 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 239000010685 fatty oil Substances 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 235000013355 food flavoring agent Nutrition 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 102000045442 glycosyltransferase activity proteins Human genes 0.000 description 1
- 108700014210 glycosyltransferase activity proteins Proteins 0.000 description 1
- 208000037824 growth disorder Diseases 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000007902 hard capsule Substances 0.000 description 1
- 230000003862 health status Effects 0.000 description 1
- 208000035861 hematochezia Diseases 0.000 description 1
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000001361 intraarterial administration Methods 0.000 description 1
- 238000010255 intramuscular injection Methods 0.000 description 1
- 239000007927 intramuscular injection Substances 0.000 description 1
- 238000010253 intravenous injection Methods 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 230000002366 lipolytic effect Effects 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 239000007937 lozenge Substances 0.000 description 1
- 235000019359 magnesium stearate Nutrition 0.000 description 1
- 229940035034 maltodextrin Drugs 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 208000013435 necrotic lesion Diseases 0.000 description 1
- 229910017464 nitrogen compound Inorganic materials 0.000 description 1
- 150000002830 nitrogen compounds Chemical class 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 239000012053 oil suspension Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 230000007505 plaque formation Effects 0.000 description 1
- 229920001592 potato starch Polymers 0.000 description 1
- 235000012015 potatoes Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 239000003380 propellant Substances 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 108010027322 single cell proteins Proteins 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000007974 sodium acetate buffer Substances 0.000 description 1
- 239000012064 sodium phosphate buffer Substances 0.000 description 1
- 239000007901 soft capsule Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 235000010356 sorbitol Nutrition 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000012192 staining solution Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 238000010254 subcutaneous injection Methods 0.000 description 1
- 239000007929 subcutaneous injection Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229960004793 sucrose Drugs 0.000 description 1
- 239000000829 suppository Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 239000010936 titanium Substances 0.000 description 1
- 229910052719 titanium Inorganic materials 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/76—Viruses; Subviral particles; Bacteriophages
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2795/00—Bacteriophages
- C12N2795/00011—Details
- C12N2795/10011—Details dsDNA Bacteriophages
- C12N2795/10311—Siphoviridae
- C12N2795/10321—Viruses as such, e.g. new isolates, mutants or their genomic sequences
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2795/00—Bacteriophages
- C12N2795/00011—Details
- C12N2795/10011—Details dsDNA Bacteriophages
- C12N2795/10311—Siphoviridae
- C12N2795/10331—Uses of virus other than therapeutic or vaccine, e.g. disinfectant
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2795/00—Bacteriophages
- C12N2795/00011—Details
- C12N2795/10011—Details dsDNA Bacteriophages
- C12N2795/10311—Siphoviridae
- C12N2795/10332—Use of virus as therapeutic agent, other than vaccine, e.g. as cytolytic agent
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Mycology (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fodder In General (AREA)
Description
クロストリジウム・パーフリンジェンスに感染するバクテリオファージの分離
バクテリオファージのスクリーニング及び単一バクテリオファージの分離
バクテリオファージの大量培養及び精製
上記のようにして選別されたバクテリオファージをクロストリジウム・パーフリンジェンス(BCCP 17−1)を用いて大量に培養し、これからバクテリオファージを精製した。
ΦCJ22の形態観察
上記のようにして精製されたバクテリオファージΦCJ22を0.01%のゼラチン溶液で希釈した後、2.5%のグルタルアルデヒド(glutaraldehyde)溶液で固定した。これを炭素コーティングされた雲母板(carbon−coated mica plate(およそ2.5mm×2.5mm))に滴下して10分間適応させた後、滅菌蒸留水で洗浄した。炭素被膜(carbon film)を銅グリッド(copper grid)上に取り付け、4%の酢酸ウラニル(uranyl acetate)で30秒〜60秒間染色し、乾燥を行った後、透過型電子顕微鏡(JEM−1011、80kV、倍率;120000〜200000倍)で検鏡した(図1)。
ΦCJ22の全体のゲノムDNAサイズの分析
前記超遠心分離を通じて精製されたバクテリオファージΦCJ22からゲノムDNAを抽出した。
ΦCJ22のタンパク質パターン分析
1010pfu/ml力価(titer)の精製されたバクテリオファージΦCJ22溶液15μlと5×SDS試料溶液3μlとを混合して5分間加熱した。その次に、15%のSDS−PAGEゲルでバクテリオファージΦCJ22の全体タンパク質を展開し、クマシーブルー(coomasie blue)染色溶液を用いてゲルを1時間常温で染色した(図3)。
ΦCJ22の全体遺伝子塩基配列の分析
上記のようにして精製したバクテリオファージΦCJ22の遺伝子的特性を調査するために、バクテリオファージΦCJ22のDNAを遺伝子分析機器FLXチタンシーケンサー(titanium sequencer)(Roche)を使用して分析した。(株)マクロジェン(Macrogen Inc.)でGSとデ・ノボアセンブリーソフトウェア(de novo assembler software)(Roche)を使用して遺伝子を組み合わせた。転写解読枠(open reading frame)は、GeneMArk.hmm、Glimmer v3.02及びFGENESBソフトウェアを使用して行われた。BLASTPとインタープロスキャン(InterProScan)プログラムを使用して転写解読枠の名前を注釈付け(annotation)した。
pHによるΦCJ22の安定性の調査
バクテリオファージΦCJ22の低いpHでの安定性を確認するために、様々なpH範囲(pH4.0、5.5、6.4、6.9、7.4、8.2、9.0及び9.8)で安定性の調査実験を実施した。
ΦCJ22の温度による安定性の調査
バクテリオファージの製品剤形のうち飼料添加剤として用いる場合、バクテリオファージの剤形の製剤過程で発生する熱に対する安定性を確認するための実験を行った。
ΦCJ22の乾燥に対する安定性の調査
バクテリオファージの製品剤形のうち飼料添加剤として用いる場合、バクテリオファージの剤形の製剤過程で発生する乾燥条件に対する安定性を確認するための実験を行った。
野生分離株クロストリジウム・パーフリンジェンスに対するΦCJ22の感染範囲の調査
実験に使用されたクロストリジウム・パーフリンジェンス(BCCP 17−1)の他に、国立獣医科学検疫院及び建国大学で分離した野生分離株クロストリジウム・パーフリンジェンス45株に対するバクテリオファージΦCJ22の溶菌活性の有無を確認した。
受託番号:KCCM11364P
受託日付:20130130
Claims (8)
- クロストリジウム・パーフリンジェンス(Clostridium perfringens)に特異的な殺菌活性を有する新規なバクテリオファージΦCJ22(KCCM11364P)。
- 請求項1に記載のバクテリオファージΦCJ22(KCCM11364P)を有効成分として含むクロストリジウム・パーフリンジェンスによる感染性疾病の予防又は治療用組成物。
- 前記感染性疾病が壊死性腸炎である、請求項2に記載のクロストリジウム・パーフリンジェンスによる感染性疾病の予防又は治療用組成物。
- 請求項1に記載のバクテリオファージΦCJ22(KCCM11364P)を有効成分として含む抗生剤。
- 請求項1に記載のバクテリオファージΦCJ22(KCCM11364P)を有効成分として含む飼料添加剤又は飲用水添加剤。
- 請求項1に記載のバクテリオファージΦCJ22(KCCM11364P)を有効成分として含む消毒剤又は洗浄剤。
- 請求項1に記載の前記バクテリオファージΦCJ22(KCCM11364P)又は請求項2に記載の組成物を、ヒトを除いた動物に投与する段階を含むヒトを除いた動物のクロストリジウム・パーフリンジェンスによる感染性疾病の予防又は治療方法。
- 前記感染性疾病が壊死性腸炎である、請求項7に記載のヒトを除いた動物のクロストリジウム・パーフリンジェンスによる感染性疾病の予防又は治療方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR10-2013-0021500 | 2013-02-27 | ||
KR1020130021500A KR101381798B1 (ko) | 2013-02-27 | 2013-02-27 | 신규 박테리오파지 및 이를 포함하는 항균 조성물 |
PCT/KR2014/001591 WO2014133322A1 (en) | 2013-02-27 | 2014-02-26 | Novel bacteriophage and antibacterial composition comprising the same |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2016510979A JP2016510979A (ja) | 2016-04-14 |
JP6130935B2 true JP6130935B2 (ja) | 2017-05-24 |
Family
ID=50656668
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2015560096A Active JP6130935B2 (ja) | 2013-02-27 | 2014-02-26 | 新規なバクテリオファージ及びこれを含む抗菌組成物 |
Country Status (10)
Country | Link |
---|---|
US (1) | US9758767B2 (ja) |
EP (1) | EP2961834B1 (ja) |
JP (1) | JP6130935B2 (ja) |
KR (1) | KR101381798B1 (ja) |
CN (1) | CN105209610B (ja) |
BR (1) | BR112015020697B1 (ja) |
ES (1) | ES2737599T3 (ja) |
PH (1) | PH12015501888B1 (ja) |
PL (1) | PL2961834T3 (ja) |
WO (1) | WO2014133322A1 (ja) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101381793B1 (ko) | 2013-02-27 | 2014-04-07 | 씨제이제일제당 (주) | 신규 박테리오파지 및 이를 포함하는 항균 조성물 |
KR101590108B1 (ko) * | 2014-04-10 | 2016-01-29 | 씨제이제일제당(주) | 신규 박테리오파지 및 이를 포함하는 조성물 |
KR101591793B1 (ko) * | 2014-04-15 | 2016-02-04 | 씨제이제일제당(주) | 신규 박테리오파지 및 이를 포함하는 조성물 |
KR101649849B1 (ko) * | 2014-12-29 | 2016-08-22 | 주식회사 인트론바이오테크놀로지 | 신규한 클로스트리디움 퍼프린젠스 박테리오파지 Clo-PEP-1 및 이의 클로스트리디움 퍼프린젠스 증식 억제 용도 |
RU2622762C1 (ru) * | 2016-03-21 | 2017-06-19 | Общество с ограниченной ответственностью "АЛФАРМ" | Антибактериальная композиция в виде суппозитория и способ ее приготовления |
KR101823860B1 (ko) | 2017-02-24 | 2018-01-31 | 주식회사 인트론바이오테크놀로지 | 신규한 클로스트리디움 퍼프린젠스 박테리오파지 Clo-PEP-2 및 이의 클로스트리디움 퍼프린젠스 균 증식 억제 용도 |
EP3372091A1 (en) * | 2017-03-07 | 2018-09-12 | Nomad Bioscience GmbH | Method of reducing contamination of an object with clostridium |
EP3395942A1 (en) * | 2017-04-25 | 2018-10-31 | IMBA-Institut für Molekulare Biotechnologie GmbH | Bi- or multi-differentiated organoid |
CN111690620B (zh) * | 2020-07-17 | 2022-05-06 | 青岛诺安百特生物技术有限公司 | 一株魏氏梭菌噬菌体、其噬菌体组合物及其应用 |
KR102586838B1 (ko) | 2021-02-25 | 2023-10-10 | 씨제이제일제당 주식회사 | 클로스트리디움 퍼프린젠스에 대한 특이적인 사멸능을 가지는 신규한 박테리오파지 및 이를 포함하는 항균 조성물 |
CN117355604A (zh) * | 2021-04-09 | 2024-01-05 | 阿帕隆有限公司 | 用于治疗产气荚膜梭菌感染的细菌噬菌体组合物 |
CN114621932B (zh) * | 2021-05-20 | 2023-11-24 | 青岛诺安百特生物技术有限公司 | 一株新型魏氏梭菌噬菌体、包含该噬菌体的噬菌体组合物及其在兔魏氏梭菌病中的应用 |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB0202556D0 (en) | 2002-02-04 | 2002-03-20 | Danisco | Novel Protein |
US7625739B2 (en) * | 2007-08-14 | 2009-12-01 | Intralytix, Inc. | Clostridium perfringens bacteriophage and uses thereof |
US7625740B2 (en) * | 2007-08-17 | 2009-12-01 | Intralytix, Inc. | Clostridium perfringens bacteriophage and uses thereof |
KR100974185B1 (ko) | 2007-08-27 | 2010-08-05 | 주식회사 인트론바이오테크놀로지 | 박테리오파지 유래 용균단백질 작용에 의해 제조된세균파쇄물 및 이를 포함하는 동물질병 예방용 백신 조성물 |
KR100910961B1 (ko) | 2007-09-13 | 2009-08-05 | 주식회사 인트론바이오테크놀로지 | 황색포도상구균의 균막 처치에 효과적인 박테리오파지 또는그것 유래의 용균단백질 |
EP2143729A1 (en) * | 2008-07-07 | 2010-01-13 | Hyglos Invest GmbH | New enzymatic active enzyme against Clostridium |
KR101151532B1 (ko) * | 2008-12-24 | 2012-05-30 | 씨제이제일제당 (주) | 신규한 박테리오파지 및 이를 포함하는 항균 조성물 |
US8962297B2 (en) | 2010-09-01 | 2015-02-24 | The United States Of America, As Represented By The Secretary Of Agriculture | Bacteriophage lytic enzymes as alternative antimicrobials |
KR20120076710A (ko) * | 2010-12-30 | 2012-07-10 | 주식회사 인트론바이오테크놀로지 | 클로스트리디움 퍼프린젠스 특이 박테리오파지 cpp-3 |
-
2013
- 2013-02-27 KR KR1020130021500A patent/KR101381798B1/ko active IP Right Grant
-
2014
- 2014-02-26 EP EP14757316.6A patent/EP2961834B1/en active Active
- 2014-02-26 JP JP2015560096A patent/JP6130935B2/ja active Active
- 2014-02-26 PL PL14757316T patent/PL2961834T3/pl unknown
- 2014-02-26 ES ES14757316T patent/ES2737599T3/es active Active
- 2014-02-26 BR BR112015020697-2A patent/BR112015020697B1/pt active IP Right Grant
- 2014-02-26 WO PCT/KR2014/001591 patent/WO2014133322A1/en active Application Filing
- 2014-02-26 CN CN201480011043.8A patent/CN105209610B/zh active Active
- 2014-02-26 US US14/767,266 patent/US9758767B2/en active Active
-
2015
- 2015-08-27 PH PH12015501888A patent/PH12015501888B1/en unknown
Also Published As
Publication number | Publication date |
---|---|
PH12015501888A1 (en) | 2015-12-07 |
US20160076003A1 (en) | 2016-03-17 |
PH12015501888B1 (en) | 2015-12-07 |
JP2016510979A (ja) | 2016-04-14 |
US9758767B2 (en) | 2017-09-12 |
EP2961834A1 (en) | 2016-01-06 |
KR101381798B1 (ko) | 2014-04-07 |
CN105209610B (zh) | 2018-04-10 |
WO2014133322A1 (en) | 2014-09-04 |
PL2961834T3 (pl) | 2019-11-29 |
EP2961834A4 (en) | 2016-08-03 |
BR112015020697A2 (pt) | 2019-11-19 |
ES2737599T3 (es) | 2020-01-15 |
BR112015020697B1 (pt) | 2023-05-09 |
CN105209610A (zh) | 2015-12-30 |
EP2961834B1 (en) | 2019-06-05 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP6110515B2 (ja) | 新規なバクテリオファージ及びこれを含む抗菌組成物 | |
JP6130935B2 (ja) | 新規なバクテリオファージ及びこれを含む抗菌組成物 | |
JP5970138B2 (ja) | 新規なバクテリオファージ及びこれを含む抗菌組成物 | |
JP6324535B2 (ja) | 新たなバクテリオファージ及びこれを含む組成物 | |
JP6069533B2 (ja) | 新規のバクテリオファージ及びこれを含む抗菌組成物 | |
JP6059827B2 (ja) | 新規なバクテリオファージ及びこれを含む抗菌組成物 | |
US10822590B2 (en) | Bacteriophage and composition comprising same | |
JP6324536B2 (ja) | 新たなバクテリオファージ及びこれを含む組成物 | |
KR101299179B1 (ko) | 신규 박테리오파지 및 이를 포함하는 항균 조성물 | |
US10925909B2 (en) | Bacteriophage and composition comprising same | |
KR102359655B1 (ko) | 신규한 리스테리아균 특이 박테리오파지 opt-lm1 및 이를 포함하는 항균 조성물 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20160726 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20161026 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20161226 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20161226 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20170411 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20170414 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 6130935 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |