JP6125752B2 - 核酸の単離 - Google Patents

核酸の単離 Download PDF

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Publication number
JP6125752B2
JP6125752B2 JP2011521638A JP2011521638A JP6125752B2 JP 6125752 B2 JP6125752 B2 JP 6125752B2 JP 2011521638 A JP2011521638 A JP 2011521638A JP 2011521638 A JP2011521638 A JP 2011521638A JP 6125752 B2 JP6125752 B2 JP 6125752B2
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buffer
solid phase
nucleic acid
solution
range
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Expired - Fee Related
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JP2011521638A
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English (en)
Japanese (ja)
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JP2011530278A5 (https=
JP2011530278A (ja
Inventor
レン チュア，イー
レン チュア，イー
ビクトリア リッチー，アリソン
ビクトリア リッチー，アリソン
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Cambridge Enterprise Ltd
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Cambridge Enterprise Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2011521638A 2008-08-08 2009-08-07 核酸の単離 Expired - Fee Related JP6125752B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0814570.8 2008-08-08
GBGB0814570.8A GB0814570D0 (en) 2008-08-08 2008-08-08 Isolation of nucleic acid
PCT/GB2009/001951 WO2010015835A1 (en) 2008-08-08 2009-08-07 Isolation of nucleic acid

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2016005930A Division JP6571544B2 (ja) 2008-08-08 2016-01-15 核酸の単離

Publications (3)

Publication Number Publication Date
JP2011530278A JP2011530278A (ja) 2011-12-22
JP2011530278A5 JP2011530278A5 (https=) 2012-09-27
JP6125752B2 true JP6125752B2 (ja) 2017-05-10

Family

ID=39790526

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2011521638A Expired - Fee Related JP6125752B2 (ja) 2008-08-08 2009-08-07 核酸の単離
JP2016005930A Expired - Fee Related JP6571544B2 (ja) 2008-08-08 2016-01-15 核酸の単離
JP2017231957A Pending JP2018042565A (ja) 2008-08-08 2017-12-01 核酸の単離

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2016005930A Expired - Fee Related JP6571544B2 (ja) 2008-08-08 2016-01-15 核酸の単離
JP2017231957A Pending JP2018042565A (ja) 2008-08-08 2017-12-01 核酸の単離

Country Status (7)

Country Link
US (2) US9422543B2 (https=)
EP (1) EP2329019B1 (https=)
JP (3) JP6125752B2 (https=)
AU (2) AU2009278915B2 (https=)
ES (1) ES2469816T3 (https=)
GB (1) GB0814570D0 (https=)
WO (1) WO2010015835A1 (https=)

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CN103890176B (zh) * 2011-09-06 2016-10-26 西北大学 制备生物材料的方法
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WO2013181651A1 (en) * 2012-06-01 2013-12-05 Omega Bio-Tek, Inc. Selective nucleic acid fragment recovery
GB201304797D0 (en) 2013-03-15 2013-05-01 Diagnostics For The Real World Ltd Apparatus and method for automated sample preparation and adaptor for use in the apparatus
EP3215620B1 (en) * 2014-11-07 2020-04-01 The Johns Hopkins University Chaotrope- and volatile-free method for purifying nucleic acids from plasma
DE102016214909B4 (de) * 2015-08-11 2018-05-17 Stem Arts Projects, Llc Tragbare Nukleinsäure-Extraktionsvorrichtung und Verfahren zu deren Verwendung
EP3397763B1 (en) 2015-12-28 2020-10-28 Koninklijke Philips N.V. Nucleic acid purification system using a single wash and elution buffer solution
US9617587B1 (en) 2016-04-04 2017-04-11 Nat Diagnostics, Inc. Isothermal amplification components and processes
WO2018156906A1 (en) * 2017-02-27 2018-08-30 miDiagnostics NV System and method for purifying and amplifying nucleic acids
WO2019135800A2 (en) 2017-09-14 2019-07-11 California Institute Of Technology Purification and detection of analytes
WO2019217570A1 (en) * 2018-05-08 2019-11-14 Waters Technologies Corporation Methods, compositions and kits useful for ph gradient cation exchange chromatography
GB201819726D0 (en) 2018-12-03 2019-01-16 Diagnostics For The Real World Ltd HCV detection
EP4010488A1 (en) * 2019-08-08 2022-06-15 Biocartis NV Novel nucleic acid purification chemistry
US11866695B2 (en) 2019-12-23 2024-01-09 California Institute Of Technology Methods and systems and related compositions for mixtures separation with a solid matrix
GB202003860D0 (en) 2020-03-17 2020-04-29 Diagnostics For The Real World Ltd Sample collection device
AU2021240470A1 (en) 2020-03-23 2022-11-24 Diagnostics For The Real World, Ltd Coronavirus detection
EP4159855A4 (en) * 2020-05-25 2024-07-24 Sekisui Medical Co., Ltd. METHOD FOR PURIFICATION OF NUCLEIC ACID
JP2025507270A (ja) * 2022-02-05 2025-03-18 ベクトン・ディキンソン・アンド・カンパニー 短い核酸標的の増幅のためにゲノムｄｎａを分離するための方法
CN114317527A (zh) * 2022-02-18 2022-04-12 欧蒙医学诊断（中国）有限公司 用于从样本中提取核酸的方法和试剂盒

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US5955261A (en) 1984-09-04 1999-09-21 Gen-Probe Incorporated Method for detecting the presence of group-specific viral mRNA in a sample
US5714380A (en) 1986-10-23 1998-02-03 Amoco Corporation Closed vessel for isolating target molecules and for performing amplification
WO1989001050A1 (en) 1987-07-31 1989-02-09 The Board Of Trustees Of The Leland Stanford Junior University Selective amplification of target polynucleotide sequences
US6090591A (en) 1987-07-31 2000-07-18 The Board Of Trustees Of The Leland Stanford Junior University Selective amplification of target polynucleotide sequences
JP2650159B2 (ja) 1988-02-24 1997-09-03 アクゾ・ノベル・エヌ・ベー 核酸増幅方法
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
AU2684488A (en) 1988-06-27 1990-01-04 Carter-Wallace, Inc. Test device and method for colored particle immunoassay
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
CA2067711C (en) 1991-05-03 2000-08-08 Daniel Lee Woodard Solid phase extraction purification of dna
US5849544A (en) 1992-07-24 1998-12-15 University Of Australia Amplification and detection process
ZA936016B (en) 1992-08-24 1994-03-10 Akzo Nv Method for nucleic acid amplification
KR100325554B1 (ko) 1993-03-26 2002-11-02 젠-프로브 인코포레이티드 사람의면역결핍 바이러스타입1의검출
US5556771A (en) 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
JP3082908B2 (ja) * 1996-07-12 2000-09-04 東洋紡績株式会社 リボ核酸の単離方法
DE69734263T2 (de) 1996-07-12 2006-07-13 Toyo Boseki K.K. Verfahren zur Isolierung von Ribonucleinsäuren.
US5981254A (en) * 1997-10-30 1999-11-09 Haemacure Corporation Process for producing thrombin from plasma
BR9815569A (pt) * 1997-12-06 2001-10-09 Dna Res Instr Ltd Método para extração de biomoléculas de material biológico
GB9725839D0 (en) 1997-12-06 1998-02-04 Baker Matthew J Isolation of nucleic acids
US6914137B2 (en) * 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
WO1999040098A1 (de) 1998-02-04 1999-08-12 Merck Patent Gmbh Verfahren zur isolierung und aufreinigung von nucleinsäuren
US6203989B1 (en) 1998-09-30 2001-03-20 Affymetrix, Inc. Methods and compositions for amplifying detectable signals in specific binding assays
DE19851156C1 (de) 1998-11-06 2000-04-27 Merck Patent Gmbh Verfahren zur Isolierung von Plasmid-DNA
DE19856064C2 (de) 1998-12-04 2000-11-30 Invitek Gmbh Universelles Verfahren zur Isolierung von DNA aus beliebigen Ausgangsmaterialien
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
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EP1560926B2 (de) * 2002-11-08 2013-08-21 STRATEC Molecular GmbH Neuartige pufferformulierungen zur isolierung, reinigung und rückgewinnung lang- und kurzkettiger nukleinsäuren
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DE102004063265B4 (de) * 2004-12-29 2012-02-02 Biontech Ag Verfahren zur Bestimmung der Funktion von Nukleinsäuresequenzen und den dadurch kodierten Expressionsprodukten
US20070184472A1 (en) * 2005-06-08 2007-08-09 Toagosei Co., Ltd Method of purifying environmental dna and method of efficiently screening for protein-encoding gene from environmental dna
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JP5268963B2 (ja) 2010-02-10 2013-08-21 アズビル株式会社 圧力測定システム及び圧力測定方法

Also Published As

Publication number Publication date
US9422543B2 (en) 2016-08-23
AU2009278915B2 (en) 2016-03-03
JP2018042565A (ja) 2018-03-22
WO2010015835A1 (en) 2010-02-11
JP2011530278A (ja) 2011-12-22
GB0814570D0 (en) 2008-09-17
EP2329019A1 (en) 2011-06-08
ES2469816T3 (es) 2014-06-20
US20110257386A1 (en) 2011-10-20
EP2329019B1 (en) 2014-03-12
AU2016203697A1 (en) 2016-06-23
JP6571544B2 (ja) 2019-09-04
US20170037394A1 (en) 2017-02-09
AU2009278915A1 (en) 2010-02-11
JP2016082980A (ja) 2016-05-19

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