AU2009278915B2 - Isolation of nucleic acid - Google Patents

Isolation of nucleic acid Download PDF

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Publication number
AU2009278915B2
AU2009278915B2 AU2009278915A AU2009278915A AU2009278915B2 AU 2009278915 B2 AU2009278915 B2 AU 2009278915B2 AU 2009278915 A AU2009278915 A AU 2009278915A AU 2009278915 A AU2009278915 A AU 2009278915A AU 2009278915 B2 AU2009278915 B2 AU 2009278915B2
Authority
AU
Australia
Prior art keywords
nucleic acid
buffer
solid phase
wash solution
buffering range
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2009278915A
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English (en)
Other versions
AU2009278915A1 (en
Inventor
Yii Leng Chua
Allyson Victoria Ritchie
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cambridge Enterprise Ltd
Original Assignee
Cambridge Enterprise Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=39790526&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AU2009278915(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Cambridge Enterprise Ltd filed Critical Cambridge Enterprise Ltd
Publication of AU2009278915A1 publication Critical patent/AU2009278915A1/en
Application granted granted Critical
Publication of AU2009278915B2 publication Critical patent/AU2009278915B2/en
Priority to AU2016203697A priority Critical patent/AU2016203697A1/en
Ceased legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2009278915A 2008-08-08 2009-08-07 Isolation of nucleic acid Ceased AU2009278915B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2016203697A AU2016203697A1 (en) 2008-08-08 2016-06-03 Isolation of nucleic acid

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0814570.8 2008-08-08
GBGB0814570.8A GB0814570D0 (en) 2008-08-08 2008-08-08 Isolation of nucleic acid
PCT/GB2009/001951 WO2010015835A1 (en) 2008-08-08 2009-08-07 Isolation of nucleic acid

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2016203697A Division AU2016203697A1 (en) 2008-08-08 2016-06-03 Isolation of nucleic acid

Publications (2)

Publication Number Publication Date
AU2009278915A1 AU2009278915A1 (en) 2010-02-11
AU2009278915B2 true AU2009278915B2 (en) 2016-03-03

Family

ID=39790526

Family Applications (2)

Application Number Title Priority Date Filing Date
AU2009278915A Ceased AU2009278915B2 (en) 2008-08-08 2009-08-07 Isolation of nucleic acid
AU2016203697A Abandoned AU2016203697A1 (en) 2008-08-08 2016-06-03 Isolation of nucleic acid

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU2016203697A Abandoned AU2016203697A1 (en) 2008-08-08 2016-06-03 Isolation of nucleic acid

Country Status (7)

Country Link
US (2) US9422543B2 (https=)
EP (1) EP2329019B1 (https=)
JP (3) JP6125752B2 (https=)
AU (2) AU2009278915B2 (https=)
ES (1) ES2469816T3 (https=)
GB (1) GB0814570D0 (https=)
WO (1) WO2010015835A1 (https=)

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CN103890176B (zh) * 2011-09-06 2016-10-26 西北大学 制备生物材料的方法
GB2561425B (en) * 2012-03-16 2019-01-16 Cambridge Entpr Ltd Apparatus for obtaining liquid from a solid phase
WO2013181651A1 (en) * 2012-06-01 2013-12-05 Omega Bio-Tek, Inc. Selective nucleic acid fragment recovery
GB201304797D0 (en) 2013-03-15 2013-05-01 Diagnostics For The Real World Ltd Apparatus and method for automated sample preparation and adaptor for use in the apparatus
EP3215620B1 (en) * 2014-11-07 2020-04-01 The Johns Hopkins University Chaotrope- and volatile-free method for purifying nucleic acids from plasma
DE102016214909B4 (de) * 2015-08-11 2018-05-17 Stem Arts Projects, Llc Tragbare Nukleinsäure-Extraktionsvorrichtung und Verfahren zu deren Verwendung
EP3397763B1 (en) 2015-12-28 2020-10-28 Koninklijke Philips N.V. Nucleic acid purification system using a single wash and elution buffer solution
US9617587B1 (en) 2016-04-04 2017-04-11 Nat Diagnostics, Inc. Isothermal amplification components and processes
WO2018156906A1 (en) * 2017-02-27 2018-08-30 miDiagnostics NV System and method for purifying and amplifying nucleic acids
WO2019135800A2 (en) 2017-09-14 2019-07-11 California Institute Of Technology Purification and detection of analytes
WO2019217570A1 (en) * 2018-05-08 2019-11-14 Waters Technologies Corporation Methods, compositions and kits useful for ph gradient cation exchange chromatography
GB201819726D0 (en) 2018-12-03 2019-01-16 Diagnostics For The Real World Ltd HCV detection
EP4010488A1 (en) * 2019-08-08 2022-06-15 Biocartis NV Novel nucleic acid purification chemistry
US11866695B2 (en) 2019-12-23 2024-01-09 California Institute Of Technology Methods and systems and related compositions for mixtures separation with a solid matrix
GB202003860D0 (en) 2020-03-17 2020-04-29 Diagnostics For The Real World Ltd Sample collection device
AU2021240470A1 (en) 2020-03-23 2022-11-24 Diagnostics For The Real World, Ltd Coronavirus detection
EP4159855A4 (en) * 2020-05-25 2024-07-24 Sekisui Medical Co., Ltd. METHOD FOR PURIFICATION OF NUCLEIC ACID
JP2025507270A (ja) * 2022-02-05 2025-03-18 ベクトン・ディキンソン・アンド・カンパニー 短い核酸標的の増幅のためにゲノムdnaを分離するための方法
CN114317527A (zh) * 2022-02-18 2022-04-12 欧蒙医学诊断(中国)有限公司 用于从样本中提取核酸的方法和试剂盒

Citations (3)

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WO2000029562A1 (de) * 1998-11-06 2000-05-25 Merck Patent Gmbh Verfahren zur isolierung von plasmid-dna
US6355792B1 (en) * 1998-02-04 2002-03-12 Merck Patent Gesellschaft Method for isolating and purifying nucleic acids
WO2004055207A1 (de) * 2002-12-13 2004-07-01 Merck Patent Gmbh Verfahren zur aufreinigung von nukleinsäuren

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JP2650159B2 (ja) 1988-02-24 1997-09-03 アクゾ・ノベル・エヌ・ベー 核酸増幅方法
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
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AU2684488A (en) 1988-06-27 1990-01-04 Carter-Wallace, Inc. Test device and method for colored particle immunoassay
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
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US5849544A (en) 1992-07-24 1998-12-15 University Of Australia Amplification and detection process
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KR100325554B1 (ko) 1993-03-26 2002-11-02 젠-프로브 인코포레이티드 사람의면역결핍 바이러스타입1의검출
US5556771A (en) 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
JP3082908B2 (ja) * 1996-07-12 2000-09-04 東洋紡績株式会社 リボ核酸の単離方法
DE69734263T2 (de) 1996-07-12 2006-07-13 Toyo Boseki K.K. Verfahren zur Isolierung von Ribonucleinsäuren.
US5981254A (en) * 1997-10-30 1999-11-09 Haemacure Corporation Process for producing thrombin from plasma
BR9815569A (pt) * 1997-12-06 2001-10-09 Dna Res Instr Ltd Método para extração de biomoléculas de material biológico
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EP1560926B2 (de) * 2002-11-08 2013-08-21 STRATEC Molecular GmbH Neuartige pufferformulierungen zur isolierung, reinigung und rückgewinnung lang- und kurzkettiger nukleinsäuren
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DE102004063265B4 (de) * 2004-12-29 2012-02-02 Biontech Ag Verfahren zur Bestimmung der Funktion von Nukleinsäuresequenzen und den dadurch kodierten Expressionsprodukten
US20070184472A1 (en) * 2005-06-08 2007-08-09 Toagosei Co., Ltd Method of purifying environmental dna and method of efficiently screening for protein-encoding gene from environmental dna
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Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6355792B1 (en) * 1998-02-04 2002-03-12 Merck Patent Gesellschaft Method for isolating and purifying nucleic acids
WO2000029562A1 (de) * 1998-11-06 2000-05-25 Merck Patent Gmbh Verfahren zur isolierung von plasmid-dna
WO2004055207A1 (de) * 2002-12-13 2004-07-01 Merck Patent Gmbh Verfahren zur aufreinigung von nukleinsäuren

Also Published As

Publication number Publication date
US9422543B2 (en) 2016-08-23
JP2018042565A (ja) 2018-03-22
WO2010015835A1 (en) 2010-02-11
JP2011530278A (ja) 2011-12-22
GB0814570D0 (en) 2008-09-17
EP2329019A1 (en) 2011-06-08
ES2469816T3 (es) 2014-06-20
US20110257386A1 (en) 2011-10-20
EP2329019B1 (en) 2014-03-12
JP6125752B2 (ja) 2017-05-10
AU2016203697A1 (en) 2016-06-23
JP6571544B2 (ja) 2019-09-04
US20170037394A1 (en) 2017-02-09
AU2009278915A1 (en) 2010-02-11
JP2016082980A (ja) 2016-05-19

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MK14 Patent ceased section 143(a) (annual fees not paid) or expired