AU2009278915B2 - Isolation of nucleic acid - Google Patents
Isolation of nucleic acid Download PDFInfo
- Publication number
- AU2009278915B2 AU2009278915B2 AU2009278915A AU2009278915A AU2009278915B2 AU 2009278915 B2 AU2009278915 B2 AU 2009278915B2 AU 2009278915 A AU2009278915 A AU 2009278915A AU 2009278915 A AU2009278915 A AU 2009278915A AU 2009278915 B2 AU2009278915 B2 AU 2009278915B2
- Authority
- AU
- Australia
- Prior art keywords
- nucleic acid
- buffer
- solid phase
- wash solution
- buffering range
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2016203697A AU2016203697A1 (en) | 2008-08-08 | 2016-06-03 | Isolation of nucleic acid |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0814570.8 | 2008-08-08 | ||
| GBGB0814570.8A GB0814570D0 (en) | 2008-08-08 | 2008-08-08 | Isolation of nucleic acid |
| PCT/GB2009/001951 WO2010015835A1 (en) | 2008-08-08 | 2009-08-07 | Isolation of nucleic acid |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2016203697A Division AU2016203697A1 (en) | 2008-08-08 | 2016-06-03 | Isolation of nucleic acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2009278915A1 AU2009278915A1 (en) | 2010-02-11 |
| AU2009278915B2 true AU2009278915B2 (en) | 2016-03-03 |
Family
ID=39790526
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2009278915A Ceased AU2009278915B2 (en) | 2008-08-08 | 2009-08-07 | Isolation of nucleic acid |
| AU2016203697A Abandoned AU2016203697A1 (en) | 2008-08-08 | 2016-06-03 | Isolation of nucleic acid |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2016203697A Abandoned AU2016203697A1 (en) | 2008-08-08 | 2016-06-03 | Isolation of nucleic acid |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US9422543B2 (https=) |
| EP (1) | EP2329019B1 (https=) |
| JP (3) | JP6125752B2 (https=) |
| AU (2) | AU2009278915B2 (https=) |
| ES (1) | ES2469816T3 (https=) |
| GB (1) | GB0814570D0 (https=) |
| WO (1) | WO2010015835A1 (https=) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103890176B (zh) * | 2011-09-06 | 2016-10-26 | 西北大学 | 制备生物材料的方法 |
| GB2561425B (en) * | 2012-03-16 | 2019-01-16 | Cambridge Entpr Ltd | Apparatus for obtaining liquid from a solid phase |
| WO2013181651A1 (en) * | 2012-06-01 | 2013-12-05 | Omega Bio-Tek, Inc. | Selective nucleic acid fragment recovery |
| GB201304797D0 (en) | 2013-03-15 | 2013-05-01 | Diagnostics For The Real World Ltd | Apparatus and method for automated sample preparation and adaptor for use in the apparatus |
| EP3215620B1 (en) * | 2014-11-07 | 2020-04-01 | The Johns Hopkins University | Chaotrope- and volatile-free method for purifying nucleic acids from plasma |
| DE102016214909B4 (de) * | 2015-08-11 | 2018-05-17 | Stem Arts Projects, Llc | Tragbare Nukleinsäure-Extraktionsvorrichtung und Verfahren zu deren Verwendung |
| EP3397763B1 (en) | 2015-12-28 | 2020-10-28 | Koninklijke Philips N.V. | Nucleic acid purification system using a single wash and elution buffer solution |
| US9617587B1 (en) | 2016-04-04 | 2017-04-11 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
| WO2018156906A1 (en) * | 2017-02-27 | 2018-08-30 | miDiagnostics NV | System and method for purifying and amplifying nucleic acids |
| WO2019135800A2 (en) | 2017-09-14 | 2019-07-11 | California Institute Of Technology | Purification and detection of analytes |
| WO2019217570A1 (en) * | 2018-05-08 | 2019-11-14 | Waters Technologies Corporation | Methods, compositions and kits useful for ph gradient cation exchange chromatography |
| GB201819726D0 (en) | 2018-12-03 | 2019-01-16 | Diagnostics For The Real World Ltd | HCV detection |
| EP4010488A1 (en) * | 2019-08-08 | 2022-06-15 | Biocartis NV | Novel nucleic acid purification chemistry |
| US11866695B2 (en) | 2019-12-23 | 2024-01-09 | California Institute Of Technology | Methods and systems and related compositions for mixtures separation with a solid matrix |
| GB202003860D0 (en) | 2020-03-17 | 2020-04-29 | Diagnostics For The Real World Ltd | Sample collection device |
| AU2021240470A1 (en) | 2020-03-23 | 2022-11-24 | Diagnostics For The Real World, Ltd | Coronavirus detection |
| EP4159855A4 (en) * | 2020-05-25 | 2024-07-24 | Sekisui Medical Co., Ltd. | METHOD FOR PURIFICATION OF NUCLEIC ACID |
| JP2025507270A (ja) * | 2022-02-05 | 2025-03-18 | ベクトン・ディキンソン・アンド・カンパニー | 短い核酸標的の増幅のためにゲノムdnaを分離するための方法 |
| CN114317527A (zh) * | 2022-02-18 | 2022-04-12 | 欧蒙医学诊断(中国)有限公司 | 用于从样本中提取核酸的方法和试剂盒 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000029562A1 (de) * | 1998-11-06 | 2000-05-25 | Merck Patent Gmbh | Verfahren zur isolierung von plasmid-dna |
| US6355792B1 (en) * | 1998-02-04 | 2002-03-12 | Merck Patent Gesellschaft | Method for isolating and purifying nucleic acids |
| WO2004055207A1 (de) * | 2002-12-13 | 2004-07-01 | Merck Patent Gmbh | Verfahren zur aufreinigung von nukleinsäuren |
Family Cites Families (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5955261A (en) | 1984-09-04 | 1999-09-21 | Gen-Probe Incorporated | Method for detecting the presence of group-specific viral mRNA in a sample |
| US5714380A (en) | 1986-10-23 | 1998-02-03 | Amoco Corporation | Closed vessel for isolating target molecules and for performing amplification |
| WO1989001050A1 (en) | 1987-07-31 | 1989-02-09 | The Board Of Trustees Of The Leland Stanford Junior University | Selective amplification of target polynucleotide sequences |
| US6090591A (en) | 1987-07-31 | 2000-07-18 | The Board Of Trustees Of The Leland Stanford Junior University | Selective amplification of target polynucleotide sequences |
| JP2650159B2 (ja) | 1988-02-24 | 1997-09-03 | アクゾ・ノベル・エヌ・ベー | 核酸増幅方法 |
| CA1340807C (en) | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
| US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
| AU2684488A (en) | 1988-06-27 | 1990-01-04 | Carter-Wallace, Inc. | Test device and method for colored particle immunoassay |
| CA2020958C (en) | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
| CA2067711C (en) | 1991-05-03 | 2000-08-08 | Daniel Lee Woodard | Solid phase extraction purification of dna |
| US5849544A (en) | 1992-07-24 | 1998-12-15 | University Of Australia | Amplification and detection process |
| ZA936016B (en) | 1992-08-24 | 1994-03-10 | Akzo Nv | Method for nucleic acid amplification |
| KR100325554B1 (ko) | 1993-03-26 | 2002-11-02 | 젠-프로브 인코포레이티드 | 사람의면역결핍 바이러스타입1의검출 |
| US5556771A (en) | 1995-02-10 | 1996-09-17 | Gen-Probe Incorporated | Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification |
| JP3082908B2 (ja) * | 1996-07-12 | 2000-09-04 | 東洋紡績株式会社 | リボ核酸の単離方法 |
| DE69734263T2 (de) | 1996-07-12 | 2006-07-13 | Toyo Boseki K.K. | Verfahren zur Isolierung von Ribonucleinsäuren. |
| US5981254A (en) * | 1997-10-30 | 1999-11-09 | Haemacure Corporation | Process for producing thrombin from plasma |
| BR9815569A (pt) * | 1997-12-06 | 2001-10-09 | Dna Res Instr Ltd | Método para extração de biomoléculas de material biológico |
| GB9725839D0 (en) | 1997-12-06 | 1998-02-04 | Baker Matthew J | Isolation of nucleic acids |
| US6914137B2 (en) * | 1997-12-06 | 2005-07-05 | Dna Research Innovations Limited | Isolation of nucleic acids |
| US6203989B1 (en) | 1998-09-30 | 2001-03-20 | Affymetrix, Inc. | Methods and compositions for amplifying detectable signals in specific binding assays |
| DE19856064C2 (de) | 1998-12-04 | 2000-11-30 | Invitek Gmbh | Universelles Verfahren zur Isolierung von DNA aus beliebigen Ausgangsmaterialien |
| US6310199B1 (en) | 1999-05-14 | 2001-10-30 | Promega Corporation | pH dependent ion exchange matrix and method of use in the isolation of nucleic acids |
| US6803196B1 (en) | 2000-10-13 | 2004-10-12 | Affymetrix, Inc. | Methods and compositions for detecting signals in binding assays using microparticles |
| EP1560926B2 (de) * | 2002-11-08 | 2013-08-21 | STRATEC Molecular GmbH | Neuartige pufferformulierungen zur isolierung, reinigung und rückgewinnung lang- und kurzkettiger nukleinsäuren |
| CA2515075C (en) | 2003-02-05 | 2012-10-02 | Iquum, Inc. | Sample processing |
| EP1498133A1 (en) | 2003-07-18 | 2005-01-19 | Aventis Pharma Deutschland GmbH | Use of a pak inhibitor for the treatment of a joint disease |
| EP1529840A1 (en) * | 2003-11-04 | 2005-05-11 | Qiagen GmbH | A rapid and low cost method for isolating nucleic acid |
| CA2567599C (en) | 2004-05-21 | 2015-07-21 | Mo Bio Laboratories, Inc. | Kits and processes for removing contaminants from nucleic acids in environmental and biological samples |
| DE102004063265B4 (de) * | 2004-12-29 | 2012-02-02 | Biontech Ag | Verfahren zur Bestimmung der Funktion von Nukleinsäuresequenzen und den dadurch kodierten Expressionsprodukten |
| US20070184472A1 (en) * | 2005-06-08 | 2007-08-09 | Toagosei Co., Ltd | Method of purifying environmental dna and method of efficiently screening for protein-encoding gene from environmental dna |
| CA2631829C (en) * | 2005-12-14 | 2012-01-24 | F. Hoffmann-La Roche Ag | New method for bisulfite treatment |
| JP2007244375A (ja) * | 2006-02-14 | 2007-09-27 | Toyobo Co Ltd | リボ核酸の分離精製方法 |
| WO2008035991A2 (en) | 2006-09-19 | 2008-03-27 | Michael Ronald Cook | A nucleic acid extraction method |
| JP5268963B2 (ja) | 2010-02-10 | 2013-08-21 | アズビル株式会社 | 圧力測定システム及び圧力測定方法 |
-
2008
- 2008-08-08 GB GBGB0814570.8A patent/GB0814570D0/en not_active Ceased
-
2009
- 2009-08-07 AU AU2009278915A patent/AU2009278915B2/en not_active Ceased
- 2009-08-07 ES ES09784897.2T patent/ES2469816T3/es active Active
- 2009-08-07 EP EP09784897.2A patent/EP2329019B1/en not_active Revoked
- 2009-08-07 WO PCT/GB2009/001951 patent/WO2010015835A1/en not_active Ceased
- 2009-08-07 US US13/057,947 patent/US9422543B2/en active Active
- 2009-08-07 JP JP2011521638A patent/JP6125752B2/ja not_active Expired - Fee Related
-
2016
- 2016-01-15 JP JP2016005930A patent/JP6571544B2/ja not_active Expired - Fee Related
- 2016-06-03 AU AU2016203697A patent/AU2016203697A1/en not_active Abandoned
- 2016-07-11 US US15/207,091 patent/US20170037394A1/en not_active Abandoned
-
2017
- 2017-12-01 JP JP2017231957A patent/JP2018042565A/ja active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6355792B1 (en) * | 1998-02-04 | 2002-03-12 | Merck Patent Gesellschaft | Method for isolating and purifying nucleic acids |
| WO2000029562A1 (de) * | 1998-11-06 | 2000-05-25 | Merck Patent Gmbh | Verfahren zur isolierung von plasmid-dna |
| WO2004055207A1 (de) * | 2002-12-13 | 2004-07-01 | Merck Patent Gmbh | Verfahren zur aufreinigung von nukleinsäuren |
Also Published As
| Publication number | Publication date |
|---|---|
| US9422543B2 (en) | 2016-08-23 |
| JP2018042565A (ja) | 2018-03-22 |
| WO2010015835A1 (en) | 2010-02-11 |
| JP2011530278A (ja) | 2011-12-22 |
| GB0814570D0 (en) | 2008-09-17 |
| EP2329019A1 (en) | 2011-06-08 |
| ES2469816T3 (es) | 2014-06-20 |
| US20110257386A1 (en) | 2011-10-20 |
| EP2329019B1 (en) | 2014-03-12 |
| JP6125752B2 (ja) | 2017-05-10 |
| AU2016203697A1 (en) | 2016-06-23 |
| JP6571544B2 (ja) | 2019-09-04 |
| US20170037394A1 (en) | 2017-02-09 |
| AU2009278915A1 (en) | 2010-02-11 |
| JP2016082980A (ja) | 2016-05-19 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGA | Letters patent sealed or granted (standard patent) | ||
| MK14 | Patent ceased section 143(a) (annual fees not paid) or expired |