JP6083877B2 - 多能性幹細胞から骨格筋細胞への分化誘導方法 - Google Patents

多能性幹細胞から骨格筋細胞への分化誘導方法 Download PDF

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JP6083877B2
JP6083877B2 JP2014521869A JP2014521869A JP6083877B2 JP 6083877 B2 JP6083877 B2 JP 6083877B2 JP 2014521869 A JP2014521869 A JP 2014521869A JP 2014521869 A JP2014521869 A JP 2014521869A JP 6083877 B2 JP6083877 B2 JP 6083877B2
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myod
cell
skeletal muscle
expression
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JP2014533491A5 (enExample
JP2014533491A (ja
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英俊 櫻井
英俊 櫻井
田中　章仁
章仁 田中
ウォルツェン、クヌート
真 池谷
真 池谷
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Kyoto University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0658—Skeletal muscle cells, e.g. myocytes, myotubes, myoblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5061—Muscle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Wood Science & Technology (AREA)
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  • Cell Biology (AREA)
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  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2014521869A 2011-11-18 2012-08-17 多能性幹細胞から骨格筋細胞への分化誘導方法 Active JP6083877B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161561586P 2011-11-18 2011-11-18
US61/561,586 2011-11-18
PCT/JP2012/071373 WO2013073246A1 (en) 2011-11-18 2012-08-17 Method of inducing differentiation from pluripotent stem cells to skeletal muscle cells

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JP2014533491A JP2014533491A (ja) 2014-12-15
JP2014533491A5 JP2014533491A5 (enExample) 2015-10-08
JP6083877B2 true JP6083877B2 (ja) 2017-02-22

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US (1) US9862929B2 (enExample)
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WO (1) WO2013073246A1 (enExample)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6566520B2 (ja) * 2013-10-29 2019-08-28 国立大学法人 熊本大学 筋疾患の治療または予防のための医薬組成物
US20160251625A1 (en) * 2013-10-30 2016-09-01 The Curators Of The University Of Missouri Method for scalable skeletal muscle lineage specification and cultivation
JP6845500B2 (ja) * 2014-12-29 2021-03-17 国立大学法人京都大学 骨格筋前駆細胞の製造方法
JP6883791B2 (ja) * 2015-01-14 2021-06-09 国立大学法人京都大学 骨格筋前駆細胞の選択方法
WO2016141234A1 (en) 2015-03-03 2016-09-09 Regents Of The University Of Minnesota Etv2 and uses thereof
CN108472318A (zh) 2015-06-30 2018-08-31 明尼苏达大学校董会 人源化心脏肌肉
US10874092B2 (en) 2015-06-30 2020-12-29 Regents Of The University Of Minnesota Humanized skeletal muscle
HK1252090A1 (zh) * 2015-08-31 2019-05-17 爱平世股份有限公司 多能干细胞制造系统和生产诱导多能干细胞的方法
US11396644B2 (en) 2016-01-31 2022-07-26 Keio University Method for promoting differentiation of pluripotent stem cells by reducing undifferentiated state thereof
US20190161731A1 (en) * 2016-04-06 2019-05-30 The General Hospital Corporation Direct reprogramming of somatic cells into myogenic cells
JP7090544B2 (ja) * 2016-04-27 2022-06-24 武田薬品工業株式会社 骨格筋前駆細胞及び骨格筋細胞の製造方法
CA3044691A1 (en) 2016-10-26 2018-05-03 Genea Biocells USA (Holdings), Inc. Improved generation of muscle lineage cells and therapeutic uses thereof
US11136555B2 (en) * 2017-03-01 2021-10-05 Elixirgen Scientific, Inc. Compositions and methods for differentiation of human pluripotent stem cells into desired cell types
ES2985769T3 (es) 2017-05-06 2024-11-07 Upside Foods Inc Composiciones y métodos para aumentar la densidad de cultivo de una biomasa celular dentro de una infraestructura de cultivo
US11479792B2 (en) 2017-07-13 2022-10-25 Upside Foods, Inc. Compositions and methods for increasing the efficiency of cell cultures used for food production
JP7548494B2 (ja) * 2018-10-30 2024-09-10 国立大学法人京都大学 細胞の製造方法
JP7533896B2 (ja) 2019-03-29 2024-08-14 公立大学法人横浜市立大学 スクリーニング方法および毒性評価法
EP4232563A4 (en) * 2020-10-26 2024-12-04 University of Massachusetts METHODS AND COMPOSITIONS FOR TREATING MUSCLE DISEASES WITH IPSC-INDUCED HUMAN SKELETAL MUSCLE STEM CELLS
CN112430624B (zh) * 2020-11-25 2023-08-18 汕头大学 一种斑马鱼肌肉特异诱导型表达载体及应用
US20240002806A1 (en) * 2022-03-10 2024-01-04 Innocent Meat GmbH Method for differentiating adult stem cells into final tissue
WO2023240152A1 (en) 2022-06-07 2023-12-14 Upside Foods, Inc. Engineering cell lines capable of proliferation in growth factor free media formulations

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6432711B1 (en) * 1993-11-03 2002-08-13 Diacrin, Inc. Embryonic stem cells capable of differentiating into desired cell lines
US6201168B1 (en) * 1999-08-20 2001-03-13 University Of Iowa Research Foundation Pathogenesis of cardiomyopathy
WO2010008100A1 (ja) * 2008-07-17 2010-01-21 国立大学法人名古屋大学 骨格筋細胞又は骨細胞の誘導法

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US9862929B2 (en) 2018-01-09
US20140370537A1 (en) 2014-12-18
WO2013073246A1 (en) 2013-05-23
JP2014533491A (ja) 2014-12-15

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