JP6083877B2 - 多能性幹細胞から骨格筋細胞への分化誘導方法 - Google Patents

多能性幹細胞から骨格筋細胞への分化誘導方法 Download PDF

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JP6083877B2
JP6083877B2 JP2014521869A JP2014521869A JP6083877B2 JP 6083877 B2 JP6083877 B2 JP 6083877B2 JP 2014521869 A JP2014521869 A JP 2014521869A JP 2014521869 A JP2014521869 A JP 2014521869A JP 6083877 B2 JP6083877 B2 JP 6083877B2
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myod
cell
skeletal muscle
expression
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JP2014533491A5 (enExample
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英俊 櫻井
英俊 櫻井
田中　章仁
章仁 田中
ウォルツェン、クヌート
真 池谷
真 池谷
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Kyoto University NUC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0658—Skeletal muscle cells, e.g. myocytes, myotubes, myoblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5061—Muscle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Rheumatology (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2014521869A 2011-11-18 2012-08-17 多能性幹細胞から骨格筋細胞への分化誘導方法 Active JP6083877B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161561586P 2011-11-18 2011-11-18
US61/561,586 2011-11-18
PCT/JP2012/071373 WO2013073246A1 (en) 2011-11-18 2012-08-17 Method of inducing differentiation from pluripotent stem cells to skeletal muscle cells

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JP2014533491A JP2014533491A (ja) 2014-12-15
JP2014533491A5 JP2014533491A5 (enExample) 2015-10-08
JP6083877B2 true JP6083877B2 (ja) 2017-02-22

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US (1) US9862929B2 (enExample)
JP (1) JP6083877B2 (enExample)
WO (1) WO2013073246A1 (enExample)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015064585A1 (ja) * 2013-10-29 2015-05-07 国立大学法人熊本大学 筋疾患の治療または予防のための医薬組成物
BR112016009803B1 (pt) * 2013-10-30 2022-02-08 The Curators Of The University Of Missouri Método para especificação e cultivo de linhagem muscular esquelética escalonável
WO2016108288A1 (ja) * 2014-12-29 2016-07-07 国立大学法人京都大学 骨格筋前駆細胞の製造方法
EP3246398B1 (en) 2015-01-14 2021-06-16 Kyoto University Method for selecting skeletal muscle progenitor cell
CN108024522A (zh) 2015-03-03 2018-05-11 明尼苏达大学董事会 Etv2及其用途
MX2018000273A (es) * 2015-06-30 2018-03-08 Univ Minnesota Musculo esqueletico humanizado.
BR112017028608A2 (pt) 2015-06-30 2018-09-04 Univ Minnesota músculo cardíaco humanizado
SG10201912931RA (en) * 2015-08-31 2020-02-27 I Peace Inc Pluripotent stem cell manufacturing system, method for inducing stem cells, floating culture method for stem cells, floating culture vessel for stem cells, method for producing induced pluripotent stem cells, and method for producing particular somatic cells from animal cells
JP6885598B2 (ja) 2016-01-31 2021-06-16 学校法人慶應義塾 未分化性低減による多能性幹細胞の分化促進法
WO2017177050A1 (en) * 2016-04-06 2017-10-12 The General Hospital Corporation Direct reprogramming of somatic cells into myogenic cells
JP7090544B2 (ja) * 2016-04-27 2022-06-24 武田薬品工業株式会社 骨格筋前駆細胞及び骨格筋細胞の製造方法
WO2018076060A1 (en) 2016-10-26 2018-05-03 Genea Ip Holdings Pty Ltd Improved generation of muscle lineage cells and therapeutic uses thereof
JP2018143239A (ja) * 2017-03-01 2018-09-20 エリクサジェン，エルエルシー． 多能性幹細胞を所望の細胞型へ効率的に分化する方法
EP4424837A3 (en) 2017-05-06 2024-12-11 Upside Foods, Inc. Compositions and methods for increasing the culture density of a cellular biomass within a cultivation infrastructure
EP3638777A4 (en) 2017-07-13 2021-05-12 Memphis Meats, Inc. COMPOSITIONS AND METHODS FOR INCREASING THE EFFICIENCY OF CELL CULTURES FOR FOOD MANUFACTURING
JP7548494B2 (ja) * 2018-10-30 2024-09-10 国立大学法人京都大学 細胞の製造方法
CN113748213A (zh) 2019-03-29 2021-12-03 公立大学法人横滨市立大学 筛选方法及毒性评价方法
EP4232563A4 (en) * 2020-10-26 2024-12-04 University of Massachusetts METHODS AND COMPOSITIONS FOR TREATING MUSCLE DISEASES WITH IPSC-INDUCED HUMAN SKELETAL MUSCLE STEM CELLS
CN112430624B (zh) * 2020-11-25 2023-08-18 汕头大学 一种斑马鱼肌肉特异诱导型表达载体及应用
US20240002806A1 (en) * 2022-03-10 2024-01-04 Innocent Meat GmbH Method for differentiating adult stem cells into final tissue
WO2023240152A1 (en) 2022-06-07 2023-12-14 Upside Foods, Inc. Engineering cell lines capable of proliferation in growth factor free media formulations

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6432711B1 (en) * 1993-11-03 2002-08-13 Diacrin, Inc. Embryonic stem cells capable of differentiating into desired cell lines
US6201168B1 (en) * 1999-08-20 2001-03-13 University Of Iowa Research Foundation Pathogenesis of cardiomyopathy
WO2010008100A1 (ja) * 2008-07-17 2010-01-21 国立大学法人名古屋大学 骨格筋細胞又は骨細胞の誘導法

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US20140370537A1 (en) 2014-12-18
WO2013073246A1 (en) 2013-05-23
US9862929B2 (en) 2018-01-09
JP2014533491A (ja) 2014-12-15

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