JP6021643B2 - 中耳粘膜様細胞シート、その製造方法及びその利用方法 - Google Patents
中耳粘膜様細胞シート、その製造方法及びその利用方法 Download PDFInfo
- Publication number
- JP6021643B2 JP6021643B2 JP2012534054A JP2012534054A JP6021643B2 JP 6021643 B2 JP6021643 B2 JP 6021643B2 JP 2012534054 A JP2012534054 A JP 2012534054A JP 2012534054 A JP2012534054 A JP 2012534054A JP 6021643 B2 JP6021643 B2 JP 6021643B2
- Authority
- JP
- Japan
- Prior art keywords
- middle ear
- mucosa
- cell sheet
- cells
- human
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/18—Internal ear or nose parts, e.g. ear-drums
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
- A61L27/3813—Epithelial cells, e.g. keratinocytes, urothelial cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3886—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/16—Otologicals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
- C12N5/062—Sensory transducers, e.g. photoreceptors; Sensory neurons, e.g. for hearing, taste, smell, pH, touch, temperature, pain
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/18—Internal ear or nose parts, e.g. ear-drums
- A61F2002/183—Ear parts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2310/00—Prostheses classified in A61F2/28 or A61F2/30 - A61F2/44 being constructed from or coated with a particular material
- A61F2310/00005—The prosthesis being constructed from a particular material
- A61F2310/00365—Proteins; Polypeptides; Degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/14—Materials or treatment for tissue regeneration for ear reconstruction or ear implants, e.g. implantable hearing aids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/30—Synthetic polymers
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Cell Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Botany (AREA)
- Dermatology (AREA)
- Epidemiology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Neurology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Urology & Nephrology (AREA)
- Vascular Medicine (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Acoustics & Sound (AREA)
- Analytical Chemistry (AREA)
- Neurosurgery (AREA)
- Heart & Thoracic Surgery (AREA)
- Otolaryngology (AREA)
- Pulmonology (AREA)
- Cardiology (AREA)
- Pharmacology & Pharmacy (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Description
本発明の一態様では、本発明の中耳粘膜様細胞シートは、換気機能、肉芽組織増生抑制機能及び/又はムチン分泌機能を有する。
本発明の一態様では、本発明の中耳粘膜様細胞シートに使用される細胞は、遺伝子導入されている。
本発明の一態様では、2以上の中耳粘膜様細胞シートが積層化されていてもよい。また、別の態様では、中耳粘膜様細胞シートに血管内皮細胞、血管内皮前駆細胞、脂肪由来細胞、間葉系幹細胞のいずれか1種、もしくは2種以上の細胞が混合した細胞シートが積層化されていてもよい。
本発明の一態様では、細胞培養支持体の細胞培養面は多孔膜のセルインサートである。
本発明の一態様では、剥離した中耳粘膜様細胞シートをさらに別の中耳粘膜様細胞シート上に積層化し、或いはその操作を繰り返すことで中耳粘膜様細胞シートを積層化している。剥離方法は、蛋白質分解酵素による処理を施されることなく細胞培養支持体から剥離する方法である。また、剥離方法は、培養終了時に培養細胞上にキャリアを密着させ、キャリアと共に剥離する方法であってもよい。
本発明の一態様では、中耳粘膜様細胞は生体組織から採取されたものであってもよい。なお、培養時に播種する細胞数が3×104〜2×105個/cm2であり得る。
本発明の一態様では、本発明の中耳粘膜様細胞シートの製造方法に使用されるポリマーは、0〜80℃の温度範囲で水和力が変化するポリマーがポリ(N−イソプロピルアクリルアミド)である。
本発明の一態様では、移植部位があらかじめフィブリングルーを施した部位であってもよい。
本発明の一態様では、中耳真珠腫、癒着性中耳炎、術後性副鼻腔嚢胞、前頭嚢胞、口腔咽頭癌、副鼻腔癌、もしくは喉頭癌の治療、または中耳腔の再建を目的としてもよい。
Claims (11)
- ヒト鼻粘膜組織からヒト鼻粘膜上皮組織を剥離し、剥離したヒト鼻粘膜上皮組織より得られたヒト鼻粘膜上皮細胞を、0〜80℃の温度範囲で水和力が変化するポリマーを表面に被覆した温度応答性細胞培養支持体上で、ポリマーの水和力が弱い温度域で培養し、その後、培養液をポリマーの水和力が強い状態となる温度に変化させることで培養したヒト中耳粘膜様細胞をシート状で剥離させることを特徴とする、繊毛を保持したヒト鼻粘膜上皮細胞を含むヒト中耳粘膜様細胞シートの製造方法であって、
前記温度応答性細胞培養支持体が多孔膜の細胞培養面を有するセルインサートであって、
前記ヒト鼻粘膜上皮細胞が下甲介粘膜、中甲介粘膜又は副鼻腔内組織由来である、ヒト中耳粘膜様細胞シートの製造方法。 - 前記ポリマーが、ポリ(N−イソプロピルアクリルアミド)である、請求項1に記載のヒト中耳粘膜様細胞シートの製造方法。
- 前記ヒト鼻粘膜上皮細胞が、剥離した前記ヒト鼻粘膜上皮組織を細胞培養基材上でエクスプラント培養して得られたヒト鼻粘上皮膜細胞である、請求項1又は2に記載のヒト中耳粘膜様細胞シートの製造方法。
- 剥離方法が培養終了時に培養細胞上にキャリアを密着させ、キャリアと共に剥離する方法である、請求項1〜3のいずれか1項記載のヒト中耳粘膜様細胞シートの製造方法。
- 前記ヒト鼻粘膜上皮細胞が、遺伝子導入されたヒト鼻粘膜上皮細胞である、請求項1〜4のいずれか1項記載のヒト中耳粘膜様細胞シートの製造方法。
- 剥離したヒト中耳粘膜様細胞シートをさらに別の中耳粘膜様細胞シート上に積層化し、
或いはその操作を繰り返すことで中耳粘膜様細胞シートを積層化する、請求項1〜5のいずれか1項記載のヒト中耳粘膜様細胞シートの製造方法。 - 前記中耳粘膜様細胞シートに血管内皮細胞、血管内皮前駆細胞、脂肪由来細胞、間葉系幹細胞のいずれか1種、もしくは2種以上の細胞が混合した細胞シートを積層化する、請求項1〜6のいずれか1項記載のヒト中耳粘膜様細胞シートの製造方法。
- 剥離方法が蛋白質分解酵素による処理を施されることなく温度応答性細胞培養支持体から剥離されたものである、請求項1〜7のいずれか1項記載のヒト中耳粘膜様細胞シートの製造方法。
- ヒト中耳粘膜様細胞が生体組織から採取されたものである、請求項1〜8のいずれか1項記載のヒト中耳粘膜様細胞シートの製造方法。
- 培養時に播種する細胞数が3×104〜2×105個/cm2である、請求項1〜9のいずれか1項記載のヒト中耳粘膜様細胞シートの製造方法。
- 培養に使用する培地がヒト血清を含むものである、請求項1〜10のいずれか1項記載のヒト中耳粘膜様細胞シートの製造方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2010226055 | 2010-09-15 | ||
JP2010226055 | 2010-09-15 | ||
PCT/JP2011/071147 WO2012036246A1 (ja) | 2010-09-15 | 2011-09-15 | 中耳粘膜様細胞シート、その製造方法及びその利用方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPWO2012036246A1 JPWO2012036246A1 (ja) | 2014-02-03 |
JP6021643B2 true JP6021643B2 (ja) | 2016-11-09 |
Family
ID=45831702
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2012534054A Active JP6021643B2 (ja) | 2010-09-15 | 2011-09-15 | 中耳粘膜様細胞シート、その製造方法及びその利用方法 |
Country Status (4)
Country | Link |
---|---|
US (2) | US20130289723A1 (ja) |
EP (1) | EP2617438B1 (ja) |
JP (1) | JP6021643B2 (ja) |
WO (1) | WO2012036246A1 (ja) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP6021643B2 (ja) | 2010-09-15 | 2016-11-09 | 学校法人東京女子医科大学 | 中耳粘膜様細胞シート、その製造方法及びその利用方法 |
JP6248617B2 (ja) * | 2013-12-25 | 2017-12-20 | 大日本印刷株式会社 | 細胞シートの製造方法 |
JP6599310B2 (ja) * | 2014-03-31 | 2019-10-30 | テルモ株式会社 | シート状細胞培養物の品質評価方法 |
JP6494393B2 (ja) * | 2015-04-21 | 2019-04-03 | 学校法人東京女子医科大学 | 子宮組織治療用細胞組成物及びその製造方法 |
WO2018154813A1 (ja) * | 2017-02-24 | 2018-08-30 | 株式会社セルシード | 組織再生培養細胞シート、製造方法及びその利用方法 |
CN112553142B (zh) * | 2020-12-11 | 2023-02-07 | 山东大学 | 一种鼻黏膜上皮细胞3d类器官及其培养方法和应用 |
TW202334387A (zh) * | 2021-12-15 | 2023-09-01 | 學校法人慈惠大學 | 新穎的鼻黏膜細胞薄片 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2000125863A (ja) * | 1998-10-21 | 2000-05-09 | Japan Tissue Engineering:Kk | 遺伝子導入細胞シート |
JP2003513633A (ja) * | 1999-11-02 | 2003-04-15 | 株式会社ディナベック研究所 | 気道上皮細胞への外因性遺伝子導入用組換えセンダイウイルスベクター |
JP2005130838A (ja) * | 2003-10-28 | 2005-05-26 | Koji Nishida | 上皮系細胞の重層化培養方法、それから得られる重層化上皮系細胞シート及びその利用方法 |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH06104061B2 (ja) | 1989-02-10 | 1994-12-21 | 花王株式会社 | 細胞培養支持体材料 |
US5726060A (en) * | 1991-09-17 | 1998-03-10 | Bridges; Michael Anthony | Method for culturing mammalian respiratory epithelial cells |
JPH05192138A (ja) | 1992-01-22 | 1993-08-03 | Kao Corp | 皮膚細胞培養法及び培養皮膚 |
US6485723B1 (en) * | 1995-02-10 | 2002-11-26 | Purdue Research Foundation | Enhanced submucosal tissue graft constructs |
KR100772276B1 (ko) | 1999-11-02 | 2007-11-01 | 가부시키가이샤 디나벡크 겐큐쇼 | 기도 상피로의 외인성 유전자 도입용 재조합센다이바이러스 벡터 |
JP4918238B2 (ja) | 2005-09-13 | 2012-04-18 | 昭和電工株式会社 | 発光装置 |
JP2007222045A (ja) * | 2006-02-22 | 2007-09-06 | Yamaguchi Univ | 中枢神経障害性疾患の回復誘発細胞 |
KR20080105792A (ko) * | 2007-06-01 | 2008-12-04 | 연세대학교 산학협력단 | 점막내강 재건용 이식물 |
JP2010226055A (ja) | 2009-03-25 | 2010-10-07 | Toshiba Mobile Display Co Ltd | 有機el表示装置 |
JP6021643B2 (ja) | 2010-09-15 | 2016-11-09 | 学校法人東京女子医科大学 | 中耳粘膜様細胞シート、その製造方法及びその利用方法 |
-
2011
- 2011-09-15 JP JP2012534054A patent/JP6021643B2/ja active Active
- 2011-09-15 EP EP11825246.9A patent/EP2617438B1/en active Active
- 2011-09-15 US US13/823,240 patent/US20130289723A1/en not_active Abandoned
- 2011-09-15 WO PCT/JP2011/071147 patent/WO2012036246A1/ja active Application Filing
-
2016
- 2016-04-14 US US15/098,604 patent/US10368980B2/en active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2000125863A (ja) * | 1998-10-21 | 2000-05-09 | Japan Tissue Engineering:Kk | 遺伝子導入細胞シート |
JP2003513633A (ja) * | 1999-11-02 | 2003-04-15 | 株式会社ディナベック研究所 | 気道上皮細胞への外因性遺伝子導入用組換えセンダイウイルスベクター |
JP2005130838A (ja) * | 2003-10-28 | 2005-05-26 | Koji Nishida | 上皮系細胞の重層化培養方法、それから得られる重層化上皮系細胞シート及びその利用方法 |
Non-Patent Citations (3)
Title |
---|
JPN6011056263; 山本和央 他: '鼻粘膜上皮細胞シート移植による中耳粘膜再生-家兎を用いた移植実験-' Otol Jpn Vol.19 No.4, 20090916, Page.623 * |
JPN6015029313; 昭和医会誌 第63巻,第2号, 2003, 第211-221頁 * |
JPN6015029316; 耳鼻咽喉科展望 第34巻,第3号, 1991, 第289-298頁 * |
Also Published As
Publication number | Publication date |
---|---|
EP2617438A4 (en) | 2015-01-21 |
US20160220356A1 (en) | 2016-08-04 |
WO2012036246A1 (ja) | 2012-03-22 |
JPWO2012036246A1 (ja) | 2014-02-03 |
EP2617438B1 (en) | 2020-02-19 |
EP2617438A1 (en) | 2013-07-24 |
US20130289723A1 (en) | 2013-10-31 |
US10368980B2 (en) | 2019-08-06 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP6021643B2 (ja) | 中耳粘膜様細胞シート、その製造方法及びその利用方法 | |
JP4716479B2 (ja) | 表皮培養細胞シート、重層化培養皮膚シート、及びそれらの製造法 | |
JP4335873B2 (ja) | 再生角膜内皮細胞シート、製造方法及びその利用方法 | |
US9889228B2 (en) | Cultured cell sheet, production method, and tissue repair method using thereof | |
US9617519B2 (en) | Method for manufacturing multilayered cell sheet, multilayered cell sheet having vascular network obtained thereby, and method of use thereof | |
KR20070015519A (ko) | 생체 조직 시트, 그것의 제조 방법, 및 그것을 이용한 이식방법 | |
WO2006003818A1 (ja) | 角膜上皮シート及びその作製方法 | |
JP2015165921A (ja) | 心筋再生用細胞シート、製造方法及びその利用方法 | |
CN106890363B (zh) | 一种工程化牙髓的制备方法 | |
JP6494393B2 (ja) | 子宮組織治療用細胞組成物及びその製造方法 | |
JP5227223B2 (ja) | 細胞培養支持体 | |
JP2004261533A (ja) | 高生着性角膜上皮代替細胞シート、製造方法及びその利用方法 | |
AU2016343292B2 (en) | Porous matrix with incorporated cells | |
WO2007029676A1 (ja) | 生体組織シート及びその作製方法 | |
JP2009225675A (ja) | 上皮系細胞シートの作製のための同種皮膚由来フィーダー細胞 | |
JP2013000121A (ja) | 上皮細胞培養用培地、それを用いた上皮細胞培養方法及びそれより得られた上皮細胞 | |
WO2023112981A1 (ja) | 新規鼻粘膜細胞シート | |
JP2005211480A (ja) | 皮膚の分離無細胞化方法、無細胞化真皮マトリックス及びその製造方法並びに無細胞化真皮マトリックスを用いた複合培養皮膚 | |
JP2004222836A (ja) | 培養真皮シートの製造方法および培養真皮シート | |
JP2023074916A (ja) | 組織再生細胞シート、製造方法及びその利用方法 | |
CN118340943A (zh) | 搭载子宫内膜间充质干细胞及上皮类器官的脱细胞羊膜支架复合体修复子宫内膜损伤 | |
CN117551604A (zh) | 搭载子宫内膜间充质干细胞及上皮类器官的脱细胞羊膜支架复合体修复子宫内膜损伤 | |
JP2010158534A (ja) | 前眼部関連細胞シート、3次元構造体、及びそれらの製造法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20140911 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20150728 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20150928 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20160209 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20160406 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20160906 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20161004 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 6021643 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |