JP6014121B2 - セルソータシステムおよび方法 - Google Patents
セルソータシステムおよび方法 Download PDFInfo
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- 210000004027 cell Anatomy 0.000 description 27
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- 238000005259 measurement Methods 0.000 description 5
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q3/00—Condition responsive control processes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/1012—Calibrating particle analysers; References therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- G01N15/01—
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects thereof, e.g. conductivity or capacity
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- G01N15/149—
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
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- G01N2015/1028—
Description
∂t=n/f1
∂t=(n+1)/f2
n=f1 /(f2 −f1 )
でなければならない。
Claims (19)
- セルソータシステムであって、
液体導管、
該液体導管中に存在する流体の流れに照射するように配置された照射源、
流体の流れに電荷を付与する荷電回路、
流体の流れから形成された1つまたは複数の液滴を受けるように配置された容器、
該容器に連結された電流検出回路、および
前記荷電回路を制御する荷電遅延制御ユニットを備え、
該荷電遅延制御ユニットは、前記電流検出回路からシグナルを受け、前記電流検出回路から受けたシグナルに基づいて荷電遅延を決定する
ことを特徴とするセルソータシステム。 - 前記電流検出回路は、前記容器に入る荷電液滴の存在を検出するための電流電圧変換器を有することを特徴とする請求項1に記載のセルソータシステム。
- 前記電流検出回路は、前記容器に入る単位時間当たりの液滴の数を検出するための積分器を有することを特徴とする請求項1に記載のセルソータシステム。
- 前記容器はドレインであることを特徴とする請求項1に記載のセルソータシステム。
- 前記ドレインは電気的に絶縁されていることを特徴とする請求項4に記載のセルソータシステム。
- 前記容器は液滴収集チューブであることを特徴とする請求項1に記載のセルソータシステム。
- 前記液滴収集チューブは電気的に絶縁されていることを特徴とする請求項6に記載のセルソータシステム。
- セルソータシステムの較正方法であって、
(a)液滴形成トランスデューサの駆動周波数を設定すること、
(b)駆動周波数で放電電荷を流体の流れに印加すること、
(c)流体の流れから形成された液滴を受ける容器で電流を測定すること、および
(d)前記容器で最大電流が検出されるまで、前記液滴形成トランスデューサの駆動振幅を変えることにより、最適駆動振幅を特定すること
を有することを特徴とする方法。 - (e)ステップ(a)の駆動周波数で放電電荷の印加を続けながら、前記容器で測定される電流が最大電流に戻るまで、前記液滴形成トランスデューサの駆動周波数を増やすことにより、第2の駆動周波数を特定することをさらに有することを特徴とする請求項8に記載の方法。
- ステップ(e)で特定された駆動周波数およびステップ(a)の駆動周波数に基づいて液滴遅延を計算することをさらに有することを特徴とする請求項9に記載の方法。
- 前記滴形成トランスデューサの駆動振幅を最適駆動振幅で一定に保持することをさらに有することを特徴とする請求項9に記載の方法。
- ステップ(a)の前に偏向板の動作を停止することをさらに有することを特徴とする請求項8に記載の方法。
- 前記容器を電気的に絶縁することをさらに有することを特徴とする請求項8に記載の方法。
- フローサイトメータ用の荷電遅延制御システムであって、
フローサイトメータの流体の流れに電荷を付与する荷電回路、
流体の流れから形成された1つまたは複数の液滴を受けるように配置されている容器に連結された電流検出回路、および
操作可能なように前記電流検出回路に連結されている荷電遅延制御ユニットであって、前記電流検出回路により測定された電流に基づいて荷電遅延を決定するように構成されている荷電遅延制御ユニット
を備えることを特徴とする荷電遅延制御システム。 - 前記電流検出回路は、前記容器に入る荷電液滴の存在を検出するための電流電圧変換器を有することを特徴とする請求項14に記載の荷電遅延制御システム。
- 前記電流検出回路は、前記容器に入る単位時間当たりの液滴の数を検出するための積分器をさらに有することを特徴とする請求項15に記載の荷電遅延制御システム。
- 前記荷電遅延制御ユニットは
(a)フローサイトメータの液滴形成トランスデューサの第1の駆動周波数で流体の流れに放電電荷を印加し、さらに
(b)前記容器で最大電流が検出されるまで、前記液滴形成トランスデューサの駆動振幅を変えることにより、最適駆動振幅を特定する
ようにさらに構成されていることを特徴とする請求項14に記載の荷電遅延制御システム。 - 前記荷電遅延制御ユニットは、第1の駆動周波数で放電電荷の印加を続けながら、前記容器で測定される電流が最大電流に戻るまで、前記液滴形成トランスデューサの駆動周波数を増やすことにより、第2の駆動周波数を特定するようにさらに構成されていることを特徴とする請求項17に記載の荷電遅延制御システム。
- 前記荷電遅延制御ユニットは、第1および第2の駆動周波数に基づいて液滴遅延を計算するようにさらに構成されていることを特徴とする請求項18に記載の荷電遅延制御システム。
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US201161480872P | 2011-04-29 | 2011-04-29 | |
US61/480,872 | 2011-04-29 | ||
PCT/US2012/028951 WO2012148584A1 (en) | 2011-04-29 | 2012-03-13 | Cell sorter system and method |
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JP2014512549A JP2014512549A (ja) | 2014-05-22 |
JP6014121B2 true JP6014121B2 (ja) | 2016-10-25 |
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US (2) | US9200334B2 (ja) |
EP (1) | EP2702133B1 (ja) |
JP (1) | JP6014121B2 (ja) |
CN (1) | CN103517980B (ja) |
AU (1) | AU2012250205B2 (ja) |
BR (1) | BR112013027173B1 (ja) |
CA (1) | CA2833341C (ja) |
ES (1) | ES2644279T3 (ja) |
WO (1) | WO2012148584A1 (ja) |
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US9453789B2 (en) | 2016-09-27 |
JP2014512549A (ja) | 2014-05-22 |
AU2012250205A1 (en) | 2013-11-07 |
ES2644279T3 (es) | 2017-11-28 |
WO2012148584A1 (en) | 2012-11-01 |
AU2012250205B2 (en) | 2016-02-04 |
EP2702133A4 (en) | 2014-12-17 |
BR112013027173B1 (pt) | 2019-08-06 |
CN103517980B (zh) | 2016-09-28 |
CA2833341C (en) | 2021-03-02 |
US9200334B2 (en) | 2015-12-01 |
EP2702133B1 (en) | 2017-08-23 |
US20160041082A1 (en) | 2016-02-11 |
CA2833341A1 (en) | 2012-11-01 |
CN103517980A (zh) | 2014-01-15 |
BR112013027173A2 (pt) | 2016-09-20 |
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US20140051064A1 (en) | 2014-02-20 |
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