JP5896360B2 - ヒト多能性幹細胞から中間中胚葉細胞への分化誘導方法 - Google Patents

ヒト多能性幹細胞から中間中胚葉細胞への分化誘導方法 Download PDF

Info

Publication number
JP5896360B2
JP5896360B2 JP2013502936A JP2013502936A JP5896360B2 JP 5896360 B2 JP5896360 B2 JP 5896360B2 JP 2013502936 A JP2013502936 A JP 2013502936A JP 2013502936 A JP2013502936 A JP 2013502936A JP 5896360 B2 JP5896360 B2 JP 5896360B2
Authority
JP
Japan
Prior art keywords
cells
cell
pluripotent stem
culture
medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2013502936A
Other languages
English (en)
Japanese (ja)
Other versions
JP2013530680A (ja
JP2013530680A5 (enExample
Inventor
健二 長船
健二 長船
伸一 前
伸一 前
正俊 梶原
正俊 梶原
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kyoto University NUC
Original Assignee
Kyoto University NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyoto University NUC filed Critical Kyoto University NUC
Publication of JP2013530680A publication Critical patent/JP2013530680A/ja
Publication of JP2013530680A5 publication Critical patent/JP2013530680A5/ja
Application granted granted Critical
Publication of JP5896360B2 publication Critical patent/JP5896360B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2013502936A 2010-07-21 2011-07-21 ヒト多能性幹細胞から中間中胚葉細胞への分化誘導方法 Active JP5896360B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US36637910P 2010-07-21 2010-07-21
US61/366,379 2010-07-21
US201161451825P 2011-03-11 2011-03-11
US61/451,825 2011-03-11
PCT/JP2011/067181 WO2012011610A1 (en) 2010-07-21 2011-07-21 Method for inducing differentiation of human pluripotent stem cell into intermediate mesoderm cell

Publications (3)

Publication Number Publication Date
JP2013530680A JP2013530680A (ja) 2013-08-01
JP2013530680A5 JP2013530680A5 (enExample) 2014-09-04
JP5896360B2 true JP5896360B2 (ja) 2016-04-13

Family

ID=45497017

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2013502936A Active JP5896360B2 (ja) 2010-07-21 2011-07-21 ヒト多能性幹細胞から中間中胚葉細胞への分化誘導方法

Country Status (4)

Country Link
US (1) US9121011B2 (enExample)
EP (1) EP2596096B1 (enExample)
JP (1) JP5896360B2 (enExample)
WO (1) WO2012011610A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023157727A1 (ja) * 2022-02-15 2023-08-24 国立大学法人神戸大学 ヒト多能性幹細胞由来ライディッヒ様細胞の作製方法及びヒト多能性幹細胞由来ライディッヒ様細胞集団

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6143268B2 (ja) 2011-12-19 2017-06-07 国立大学法人京都大学 ヒト多能性幹細胞から中間中胚葉細胞への分化誘導方法
US9334475B2 (en) * 2012-04-06 2016-05-10 Kyoto University Method for inducing erythropoietin-producing cell
KR20130119683A (ko) * 2012-04-24 2013-11-01 사회복지법인 삼성생명공익재단 줄기세포 배양 배지 및 이를 이용한 줄기세포의 배양방법
EP2976044A4 (en) * 2013-03-21 2016-11-23 Univ Columbia COMPOSITIONS AND METHODS FOR THE DENTAL GENERATION
EP2985344B1 (en) * 2013-04-12 2018-07-18 Kyoto University Method for inducing alveolar epithelium progenitor cells
CA2915085C (en) 2013-06-11 2021-04-27 Kyoto University Method for producing renal progenitor cells and drug comprising the same
EP3011014B1 (en) * 2013-06-14 2024-11-13 The University of Queensland Renal progenitor cells
BR112015030685A2 (pt) 2013-07-29 2017-07-25 Hoffmann La Roche método para diferenciar células-tronco, células, biobanco de células, uso das células e composição terapêutica
WO2015056756A1 (ja) * 2013-10-18 2015-04-23 国立大学法人熊本大学 多能性幹細胞からの腎臓誘導法
CA2968655C (en) 2014-11-25 2021-11-16 President And Fellows Of Harvard College Methods for generation of podocytes from pluripotent stem cells and cells produced by the same
AU2014277667B2 (en) 2014-12-15 2022-07-14 The University Of Queensland Differentiation of pluripotent stem cells to form renal organoids
WO2016143803A1 (ja) 2015-03-06 2016-09-15 国立大学法人京都大学 肺胞上皮細胞の分化誘導法
US10563171B2 (en) 2015-06-29 2020-02-18 Kyoto University Method for inducing differentiation of pluripotent stem cells into germ cells
JP6646311B2 (ja) * 2015-07-17 2020-02-14 国立大学法人京都大学 多能性幹細胞から中胚葉前駆細胞および血液血管前駆細胞への分化誘導法
CN108473962B (zh) * 2015-09-08 2022-04-26 (由卫生与公众服务部部长代表的)美利坚合众国 临床级别视网膜色素上皮细胞的可再现的分化方法
CN108138136B (zh) 2015-09-11 2022-02-11 安斯泰来制药株式会社 制造肾前体细胞的方法
CN105850980B (zh) * 2016-04-14 2018-06-12 广州赛莱拉干细胞科技股份有限公司 一种角膜缘干细胞的冻存液及冻存方法
EP3519558B1 (en) 2016-09-28 2023-08-09 Organovo, Inc. Use of engineered renal tissues in assays
WO2018216743A1 (ja) 2017-05-25 2018-11-29 国立大学法人京都大学 中間中胚葉細胞から腎前駆細胞への分化誘導方法、および多能性幹細胞から腎前駆細胞への分化誘導方法
US20210123027A1 (en) 2018-10-10 2021-04-29 National University Corporation Tottori University Method for producing human induced pluripotent stem cells containing exogenous chromosome
EP3875578A4 (en) * 2018-10-31 2022-08-10 Kyoto University METHOD FOR GENERATING PLURIPOTENT STEM CELLS WITH RELEASED DIFFERENTIATION RESISTANCE TO MESENDODERM
JP7489377B2 (ja) 2019-03-29 2024-05-23 株式会社カネカ 多能性幹細胞を含む細胞集団及びその製造方法
CN110656089B (zh) * 2019-10-15 2021-02-09 上海捷易生物科技有限公司 一种将iPS细胞定向诱导分化到成熟神经元的方法
US11591381B2 (en) 2020-11-30 2023-02-28 Crispr Therapeutics Ag Gene-edited natural killer cells
EP4271798A1 (en) 2020-12-30 2023-11-08 CRISPR Therapeutics AG Compositions and methods for differentiating stem cells into nk cells

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
WO2007069666A1 (ja) 2005-12-13 2007-06-21 Kyoto University 核初期化因子
CA2676044C (en) * 2007-01-30 2019-10-22 University Of Georgia Research Foundation, Inc. Early mesoderm cells, a stable population of mesendoderm cells that has utility for generation of endoderm and mesoderm lineages and multipotent migratory cells (mmc)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023157727A1 (ja) * 2022-02-15 2023-08-24 国立大学法人神戸大学 ヒト多能性幹細胞由来ライディッヒ様細胞の作製方法及びヒト多能性幹細胞由来ライディッヒ様細胞集団

Also Published As

Publication number Publication date
JP2013530680A (ja) 2013-08-01
EP2596096A1 (en) 2013-05-29
EP2596096B1 (en) 2018-01-31
US20130122536A1 (en) 2013-05-16
WO2012011610A1 (en) 2012-01-26
EP2596096A4 (en) 2014-01-08
US9121011B2 (en) 2015-09-01

Similar Documents

Publication Publication Date Title
JP5896360B2 (ja) ヒト多能性幹細胞から中間中胚葉細胞への分化誘導方法
JP7282304B2 (ja) 新規ドーパミン産生神経前駆細胞の誘導方法
JP6143268B2 (ja) ヒト多能性幹細胞から中間中胚葉細胞への分化誘導方法
JP7166631B2 (ja) 新規軟骨細胞誘導方法
JP2022037232A (ja) ドーパミン産生神経前駆細胞の製造方法
JP5896421B2 (ja) 多能性幹細胞から骨格筋または骨格筋前駆細胞への分化誘導法
JP6824526B2 (ja) 大脳皮質ニューロンの誘導方法
JP2013528356A (ja) ヒト人工多能性幹細胞の選択方法
JP6090937B2 (ja) 新規ドーパミン産生神経前駆細胞マーカー
JP6646311B2 (ja) 多能性幹細胞から中胚葉前駆細胞および血液血管前駆細胞への分化誘導法
JP5995247B2 (ja) 多能性幹細胞から樹状細胞を製造する方法
EP2938721A1 (en) Method for inducing astrocytes
JP5842289B2 (ja) 効率的な内皮細胞の誘導方法
JP7072756B2 (ja) 多能性幹細胞から中胚葉前駆細胞および血液血管前駆細胞への分化誘導法
WO2022102742A1 (ja) 骨格筋系譜細胞および骨格筋幹細胞の高効率純化用細胞表面マーカーおよびその利用

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140717

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20140717

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20150818

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20151015

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20160126

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20160222

R150 Certificate of patent or registration of utility model

Ref document number: 5896360

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250