JP5866601B2 - 医薬組成物の製造方法 - Google Patents

医薬組成物の製造方法 Download PDF

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JP5866601B2
JP5866601B2 JP2011514618A JP2011514618A JP5866601B2 JP 5866601 B2 JP5866601 B2 JP 5866601B2 JP 2011514618 A JP2011514618 A JP 2011514618A JP 2011514618 A JP2011514618 A JP 2011514618A JP 5866601 B2 JP5866601 B2 JP 5866601B2
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cells
tsg
msc
protein
cell
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JP2011525355A5 (enExample
JP2011525355A (ja
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プロコップ，ダーウィン，ジェイ．
イ，リャン，ファ
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Texas A&M University System
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/525—Tumour necrosis factor [TNF]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/25—Tumour necrosing factors [TNF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50—Cell markers; Cell surface determinants
    • C12N2501/585—Integrins

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Rheumatology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Cell Biology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Cardiology (AREA)
  • Urology & Nephrology (AREA)
  • Vascular Medicine (AREA)
  • Pain & Pain Management (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
JP2011514618A 2008-06-18 2009-06-18 医薬組成物の製造方法 Expired - Fee Related JP5866601B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US7373908P 2008-06-18 2008-06-18
US61/073,739 2008-06-18
PCT/US2009/003657 WO2009154770A2 (en) 2008-06-18 2009-06-18 Mesenchymal stem cells, compositions, and methods for treatment of cardiac tissue damage

Related Child Applications (1)

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JP2015119567A Division JP2015199755A (ja) 2008-06-18 2015-06-12 医薬組成物

Publications (3)

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JP2011525355A JP2011525355A (ja) 2011-09-22
JP2011525355A5 JP2011525355A5 (enExample) 2012-08-02
JP5866601B2 true JP5866601B2 (ja) 2016-02-17

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JP2011514618A Expired - Fee Related JP5866601B2 (ja) 2008-06-18 2009-06-18 医薬組成物の製造方法
JP2015119567A Pending JP2015199755A (ja) 2008-06-18 2015-06-12 医薬組成物

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US (1) US10035834B2 (enExample)
EP (2) EP3029061B1 (enExample)
JP (2) JP5866601B2 (enExample)
WO (1) WO2009154770A2 (enExample)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9878003B2 (en) 2006-03-06 2018-01-30 The University Of Manchester Method of treating bone disorders using TSG-6
AU2010303469A1 (en) * 2009-10-06 2012-05-03 The Cohen Mcniece Foundation Preparation and use of stromal cells for treatment of cardiac diseases
EP2537922B1 (en) * 2010-02-18 2021-04-21 Kang Stem Biotech Co., Ltd. Cd49f promoting proliferation, multipotency and reprogramming of adult stem cells through pi3k/akt/gsk3 pathway
KR101869077B1 (ko) * 2010-05-03 2018-06-22 더 텍사스 에이 & 엠 유니버시티 시스템 눈 손상 및 질환 치료용 성체 줄기 세포／전구 세포 및 줄기 세포 단백질
WO2013035101A1 (en) 2011-09-11 2013-03-14 Minovia Therapeutics Ltd. Compositions of functional mitochondria and uses thereof
EP2626369A1 (en) * 2011-11-24 2013-08-14 Fundació Institut d'Investigació Biomèdica de Bellvitge (IDIBELL) Engineered mesenchymal stem cells and their therapeutic use
RU2482183C1 (ru) * 2011-12-02 2013-05-20 Государственное научное учреждение Всероссийский научно-исследовательский институт экспериментальной ветеринарии им. Я.Р. Коваленко Российской академии сельскохозяйственных наук (ГНУ ВИЭВ Россельхозакадемии) КУЛЬТУРА МУЛЬТИПОТЕНТНЫХ МЕЗЕНХИМНЫХ СТВОЛОВЫХ КЛЕТОК, ВЫДЕЛЕННЫХ ИЗ КОСТНОГО МОЗГА КРУПНОГО РОГАТОГО СКОТА (Medulla ossium Bos taurus), ДЛЯ ВЕТЕРИНАРИИ, КЛЕТОЧНОЙ И ТКАНЕВОЙ ИНЖЕНЕРИИ
RU2482182C1 (ru) * 2011-12-02 2013-05-20 Государственное научное учреждение Всероссийский научно-исследовательский институт экспериментальной ветеринарии им. Я.Р. Коваленко Российской академии сельскохозяйственных наук (ГНУ ВИЭВ Россельхозакадемии) КУЛЬТУРА МУЛЬТИПОТЕНТНЫХ МЕЗЕНХИМНЫХ СТВОЛОВЫХ КЛЕТОК, ВЫДЕЛЕННЫХ ИЗ ЖИРОВОЙ ТКАНИ КРУПНОГО РОГАТОГО СКОТА (Textus adiposus Bos taurus), ДЛЯ ВЕТЕРИНАРИИ, КЛЕТОЧНОЙ И ТКАНЕВОЙ ИНЖЕНЕРИИ
WO2013171752A1 (en) 2012-05-16 2013-11-21 Minovia Therapeutics Ltd. Compositions and methods for inducing angiogenesis
EP2908910A4 (en) * 2012-10-17 2016-06-29 Texas A & M Univ Sys TREATMENT OF BRAIN DAMAGE OR TRAUMA WITH TSG-6 PROTEIN
WO2016081032A2 (en) * 2014-07-11 2016-05-26 The Texas A&M University System Mesenchymal stem cells derived from induced pluripotent stem cells
US20170204151A1 (en) * 2014-07-28 2017-07-20 Darwin J. Prockop Mesenchymal Stem Cells Expressing Biomarkers that Predict the Effectiveness of Mesenchymal Stem Cells for Treating Diseases and Disorders
WO2016135723A1 (en) 2015-02-26 2016-09-01 Minovia Therapeutics Ltd. Mammalian cells enriched with functional mitochondria
KR101816246B1 (ko) 2016-09-07 2018-01-08 에스씨엠생명과학 주식회사 염증 자극된 중간엽 줄기세포를 포함하는 면역질환 또는 염증 질환의 예방 또는 치료용 약학적 조성물
EP3551748B1 (en) * 2016-12-12 2022-07-13 Health and Biotech France (H&B France) Perinatal tissue derived mesenchymal stem cells: method of preparation and uses thereof
JP7237301B2 (ja) * 2016-12-16 2023-03-13 有限会社大阪空気機械サービス 組織治癒剤
EP3563857B1 (en) * 2016-12-28 2023-04-26 Two Cells Co., Ltd Repair agent for living tissue damage comprising mesenchymal stem cells, and method for producing said repair agent
CN110423721B (zh) * 2018-05-01 2024-02-27 云南济慈再生医学研究院有限公司 一种年轻化的修复型成纤维细胞的制备方法及其应用
WO2020021538A1 (en) 2018-07-22 2020-01-30 Minovia Therapeutics Ltd. Mitochondrial augmentation therapy of brain diseases
JP7458368B2 (ja) 2018-07-22 2024-03-29 ミノヴィア セラピューティクス リミテッド 筋疾患のミトコンドリア増強療法
EP3823645B1 (en) 2018-07-22 2024-07-03 Minovia Therapeutics Ltd. Mitochondrial augmentation therapy of renal diseases
US12239672B2 (en) 2018-07-22 2025-03-04 Minovia Therapeutics Ltd. Mitochondrial augmentation therapy of ocular diseases
CA3149572A1 (en) * 2019-08-30 2021-03-04 Ashlee WATTS Xenogen-free mesenchymal stem cell compositions and methods of use
EP4169518A4 (en) * 2020-07-17 2024-01-03 Lg Chem, Ltd. Composition for preventing or treating osteoarthritis, comprising mesenchymal stem cell expressing tumor necrosis factor-inducible gene 6
KR20250085398A (ko) * 2023-12-05 2025-06-12 재단법인 아산사회복지재단 턱관절 질환의 예방 또는 치료용 줄기세포 치료제 및 이의 제조방법

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8308235D0 (en) 1983-03-25 1983-05-05 Celltech Ltd Polypeptides
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US5272065A (en) 1983-10-20 1993-12-21 Research Foundation Of State University Of New York Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA
US4978745A (en) 1987-11-23 1990-12-18 Centocor, Inc. Immunoreactive heterochain antibodies
US4975369A (en) 1988-04-21 1990-12-04 Eli Lilly And Company Recombinant and chimeric KS1/4 antibodies directed against a human adenocarcinoma antigen
US5087617A (en) 1989-02-15 1992-02-11 Board Of Regents, The University Of Texas System Methods and compositions for treatment of cancer using oligonucleotides
WO1992012175A1 (en) * 1991-01-14 1992-07-23 New York University Cytokine-induced protein, tsg-6, dna coding therefor and uses thereof
AU2322392A (en) 1991-07-03 1993-02-11 Regeneron Pharmaceuticals, Inc. Method and assay system for neurotrophin activity
WO1995021381A1 (en) 1994-02-01 1995-08-10 The Rockefeller University Methods and agents for measuring and controlling multidrug resistance
US6313091B1 (en) 1995-07-20 2001-11-06 New York University Pharmaceutical compositions containing TSG-6 for treating inflammatory diseases and cancer-related pathologies and method
KR20000075748A (ko) 1997-02-26 2000-12-26 마빈 씨. 구트리 알츠하이머 병의 치료 또는 예방에 유효한 약물을 선별하기 위한 유전자 변형동물 및 세포계
DK1007631T4 (da) 1997-07-14 2009-04-27 Osiris Therapeutics Inc Hjertemuskelregeneration ved anvendelse af mesenkymale stamceller
ES2314786T3 (es) * 1998-04-02 2009-03-16 Genentech, Inc. Uso de interferon gamma para el tratamiento de la hipertrofia cardiaca.
US20040214836A1 (en) * 1998-05-29 2004-10-28 Cheresh David A. Method of treatment of myocardial infarction
IL141588A0 (en) * 1998-09-15 2002-03-10 M & E Biotech As Method for down-regulating osteoprotegerin ligand activity
US6872546B1 (en) * 1998-12-23 2005-03-29 Human Genome Sciences, Inc. Hyaluronan-binding proteins and encoding genes
US6610503B1 (en) 1999-03-17 2003-08-26 Xenogen Corporation Animal models for predicting sepsis mortality
WO2000077246A2 (en) 1999-06-14 2000-12-21 Novo Nordisk A/S A METHOD FOR IDENTIFYING A DRUG CANDIDATE HAVING AN INHIBITORY ACTION ON FVII-TF ACTIVITY, AND FVIIa/TF ACTIVITY INHIBITING COMPOUNDS
US6632979B2 (en) 2000-03-16 2003-10-14 Genentech, Inc. Rodent HER2 tumor model
EP1138694A1 (en) * 2000-03-30 2001-10-04 University Of Manitoba Hirulog-like peptide
US6905839B2 (en) 2002-07-31 2005-06-14 Takashi Furuta In vivo phenotyping for human cytochrome P450 3A activity
US20070010008A1 (en) 2005-06-29 2007-01-11 Tissuetech, Inc. Ex vivo expansion of primary animal cells
BRPI0414961A (pt) * 2003-10-03 2006-11-07 Keiichi Fukuda processo de indução da diferenciação de células tronco em células do miocárdio
US9002545B2 (en) 2011-01-07 2015-04-07 Wabtec Holding Corp. Data improvement system and method

Also Published As

Publication number Publication date
JP2015199755A (ja) 2015-11-12
US20100278790A1 (en) 2010-11-04
US10035834B2 (en) 2018-07-31
EP3029061A1 (en) 2016-06-08
JP2011525355A (ja) 2011-09-22
EP3029061B1 (en) 2017-09-13
EP2294183A4 (en) 2012-08-15
EP2294183A2 (en) 2011-03-16
WO2009154770A3 (en) 2010-03-18
WO2009154770A2 (en) 2009-12-23
EP2294183B1 (en) 2015-12-16

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