JP5856814B2 - 画像解析方法および画像解析装置 - Google Patents

画像解析方法および画像解析装置 Download PDF

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Publication number
JP5856814B2
JP5856814B2 JP2011246462A JP2011246462A JP5856814B2 JP 5856814 B2 JP5856814 B2 JP 5856814B2 JP 2011246462 A JP2011246462 A JP 2011246462A JP 2011246462 A JP2011246462 A JP 2011246462A JP 5856814 B2 JP5856814 B2 JP 5856814B2
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JP
Japan
Prior art keywords
image
analysis
frame
data
region
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Expired - Fee Related
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JP2011246462A
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English (en)
Japanese (ja)
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JP2013104674A (ja
JP2013104674A5 (enExample
Inventor
明美 鈴木
明美 鈴木
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Olympus Corp
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Olympus Corp
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Publication date
Application filed by Olympus Corp filed Critical Olympus Corp
Priority to JP2011246462A priority Critical patent/JP5856814B2/ja
Priority to EP12848351.8A priority patent/EP2778659A4/en
Priority to PCT/JP2012/075841 priority patent/WO2013069393A1/ja
Priority to CN201280055334.8A priority patent/CN103917860B/zh
Publication of JP2013104674A publication Critical patent/JP2013104674A/ja
Priority to US14/274,716 priority patent/US9262667B2/en
Publication of JP2013104674A5 publication Critical patent/JP2013104674A5/ja
Application granted granted Critical
Publication of JP5856814B2 publication Critical patent/JP5856814B2/ja
Expired - Fee Related legal-status Critical Current
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    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/698—Matching; Classification
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/18—Arrangements with more than one light path, e.g. for comparing two specimens
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/97—Determining parameters from multiple pictures
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/695—Preprocessing, e.g. image segmentation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy

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  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Chemical & Material Sciences (AREA)
  • Theoretical Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Multimedia (AREA)
  • Health & Medical Sciences (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Image Analysis (AREA)
JP2011246462A 2011-11-10 2011-11-10 画像解析方法および画像解析装置 Expired - Fee Related JP5856814B2 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2011246462A JP5856814B2 (ja) 2011-11-10 2011-11-10 画像解析方法および画像解析装置
EP12848351.8A EP2778659A4 (en) 2011-11-10 2012-10-04 IMAGE ANALYSIS METHOD AND IMAGE ANALYSIS DEVICE
PCT/JP2012/075841 WO2013069393A1 (ja) 2011-11-10 2012-10-04 画像解析方法および画像解析装置
CN201280055334.8A CN103917860B (zh) 2011-11-10 2012-10-04 图像解析方法和图像解析装置
US14/274,716 US9262667B2 (en) 2011-11-10 2014-05-10 Image analysis method and image analysis apparatus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2011246462A JP5856814B2 (ja) 2011-11-10 2011-11-10 画像解析方法および画像解析装置

Publications (3)

Publication Number Publication Date
JP2013104674A JP2013104674A (ja) 2013-05-30
JP2013104674A5 JP2013104674A5 (enExample) 2014-12-25
JP5856814B2 true JP5856814B2 (ja) 2016-02-10

Family

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Family Applications (1)

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JP2011246462A Expired - Fee Related JP5856814B2 (ja) 2011-11-10 2011-11-10 画像解析方法および画像解析装置

Country Status (5)

Country Link
US (1) US9262667B2 (enExample)
EP (1) EP2778659A4 (enExample)
JP (1) JP5856814B2 (enExample)
CN (1) CN103917860B (enExample)
WO (1) WO2013069393A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104808327B (zh) * 2015-05-15 2017-10-27 华东理工大学 一种用于细胞操纵的显微镜
WO2018003069A1 (ja) 2016-06-30 2018-01-04 オリンパス株式会社 観察支援装置および蛍光観察システム
WO2020243698A1 (en) * 2019-05-30 2020-12-03 University Of Utah Research Foundation Interferometric fluorescent cross correlation spectroscopy
CN111589478B (zh) * 2020-06-05 2021-06-29 珠海市尚维高科生物技术有限公司 一种双通道实时荧光定量pcr仪光路系统及检测方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4830087B2 (ja) 2005-09-27 2011-12-07 国立大学法人北海道大学 光信号解析装置
JP4879543B2 (ja) * 2005-09-28 2012-02-22 オリンパス株式会社 蛍光分光分析装置
JP5489469B2 (ja) * 2007-01-16 2014-05-14 オリンパス株式会社 蛍光信号解析装置および蛍光信号解析方法
JP5354938B2 (ja) * 2007-05-02 2013-11-27 オリンパス株式会社 蛍光顕微鏡装置
DE102007052551B4 (de) * 2007-10-30 2019-06-19 Carl Zeiss Microscopy Gmbh Verfahren zur Durchführung einer Rasterbildkorrelationsspektroskopiemessung sowie Steuereinheit, Laser-Scanning-Mikroskop und Computerprogramm
JP5468805B2 (ja) * 2009-03-31 2014-04-09 オリンパス株式会社 画像解析方法および画像解析装置
JP5566055B2 (ja) * 2009-07-10 2014-08-06 オリンパス株式会社 画像解析方法および画像解析装置
JP5508809B2 (ja) * 2009-10-15 2014-06-04 オリンパス株式会社 画像解析方法および画像解析装置
JP5508808B2 (ja) * 2009-10-15 2014-06-04 オリンパス株式会社 画像解析方法および画像解析装置

Also Published As

Publication number Publication date
JP2013104674A (ja) 2013-05-30
US9262667B2 (en) 2016-02-16
EP2778659A4 (en) 2015-06-17
US20140247976A1 (en) 2014-09-04
EP2778659A1 (en) 2014-09-17
CN103917860B (zh) 2017-02-15
CN103917860A (zh) 2014-07-09
WO2013069393A1 (ja) 2013-05-16

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