CN103917860B - 图像解析方法和图像解析装置 - Google Patents

图像解析方法和图像解析装置 Download PDF

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Publication number
CN103917860B
CN103917860B CN201280055334.8A CN201280055334A CN103917860B CN 103917860 B CN103917860 B CN 103917860B CN 201280055334 A CN201280055334 A CN 201280055334A CN 103917860 B CN103917860 B CN 103917860B
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CN
China
Prior art keywords
image
frame
analysis
area
analysis area
Prior art date
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Expired - Fee Related
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CN201280055334.8A
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English (en)
Chinese (zh)
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CN103917860A (zh
Inventor
铃木明美
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Olympus Corp
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Olympus Corp
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Publication date
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Publication of CN103917860A publication Critical patent/CN103917860A/zh
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Publication of CN103917860B publication Critical patent/CN103917860B/zh
Expired - Fee Related legal-status Critical Current
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    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/698—Matching; Classification
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/18—Arrangements with more than one light path, e.g. for comparing two specimens
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/97—Determining parameters from multiple pictures
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/695—Preprocessing, e.g. image segmentation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy

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  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Chemical & Material Sciences (AREA)
  • Theoretical Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Multimedia (AREA)
  • Health & Medical Sciences (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Image Analysis (AREA)
CN201280055334.8A 2011-11-10 2012-10-04 图像解析方法和图像解析装置 Expired - Fee Related CN103917860B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2011-246462 2011-11-10
JP2011246462A JP5856814B2 (ja) 2011-11-10 2011-11-10 画像解析方法および画像解析装置
PCT/JP2012/075841 WO2013069393A1 (ja) 2011-11-10 2012-10-04 画像解析方法および画像解析装置

Publications (2)

Publication Number Publication Date
CN103917860A CN103917860A (zh) 2014-07-09
CN103917860B true CN103917860B (zh) 2017-02-15

Family

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Family Applications (1)

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CN201280055334.8A Expired - Fee Related CN103917860B (zh) 2011-11-10 2012-10-04 图像解析方法和图像解析装置

Country Status (5)

Country Link
US (1) US9262667B2 (enExample)
EP (1) EP2778659A4 (enExample)
JP (1) JP5856814B2 (enExample)
CN (1) CN103917860B (enExample)
WO (1) WO2013069393A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104808327B (zh) * 2015-05-15 2017-10-27 华东理工大学 一种用于细胞操纵的显微镜
WO2018003069A1 (ja) 2016-06-30 2018-01-04 オリンパス株式会社 観察支援装置および蛍光観察システム
WO2020243698A1 (en) * 2019-05-30 2020-12-03 University Of Utah Research Foundation Interferometric fluorescent cross correlation spectroscopy
CN111589478B (zh) * 2020-06-05 2021-06-29 珠海市尚维高科生物技术有限公司 一种双通道实时荧光定量pcr仪光路系统及检测方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011046211A1 (ja) * 2009-10-15 2011-04-21 オリンパス株式会社 画像解析方法および画像解析装置

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4830087B2 (ja) 2005-09-27 2011-12-07 国立大学法人北海道大学 光信号解析装置
JP4879543B2 (ja) * 2005-09-28 2012-02-22 オリンパス株式会社 蛍光分光分析装置
JP5489469B2 (ja) * 2007-01-16 2014-05-14 オリンパス株式会社 蛍光信号解析装置および蛍光信号解析方法
JP5354938B2 (ja) * 2007-05-02 2013-11-27 オリンパス株式会社 蛍光顕微鏡装置
DE102007052551B4 (de) * 2007-10-30 2019-06-19 Carl Zeiss Microscopy Gmbh Verfahren zur Durchführung einer Rasterbildkorrelationsspektroskopiemessung sowie Steuereinheit, Laser-Scanning-Mikroskop und Computerprogramm
JP5468805B2 (ja) * 2009-03-31 2014-04-09 オリンパス株式会社 画像解析方法および画像解析装置
JP5566055B2 (ja) * 2009-07-10 2014-08-06 オリンパス株式会社 画像解析方法および画像解析装置
JP5508809B2 (ja) * 2009-10-15 2014-06-04 オリンパス株式会社 画像解析方法および画像解析装置

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011046211A1 (ja) * 2009-10-15 2011-04-21 オリンパス株式会社 画像解析方法および画像解析装置

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Detecting Protein Complexes in Living Cells from Laser Scanning Confocal Image Sequences by the Cross Correlation Raster Image Spectroscopy Method;Michelle A. Digman et.al.;《Biophysical Journal》;20090131;全文 *
Measuring Fast Dynamics in Solutions and Cells with a Laser Scanning Microscope;Michelle A. Digman et.al.;《Biophysical Journal》;20050831;摘要，1319页-1322页，图1，图2 *

Also Published As

Publication number Publication date
JP2013104674A (ja) 2013-05-30
US9262667B2 (en) 2016-02-16
EP2778659A4 (en) 2015-06-17
US20140247976A1 (en) 2014-09-04
JP5856814B2 (ja) 2016-02-10
EP2778659A1 (en) 2014-09-17
CN103917860A (zh) 2014-07-09
WO2013069393A1 (ja) 2013-05-16

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Granted publication date: 20170215

Termination date: 20191004