JP5841997B2 - 内因性にタグ付けされたタンパク質を生成するための方法 - Google Patents

内因性にタグ付けされたタンパク質を生成するための方法 Download PDF

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JP5841997B2
JP5841997B2 JP2013505078A JP2013505078A JP5841997B2 JP 5841997 B2 JP5841997 B2 JP 5841997B2 JP 2013505078 A JP2013505078 A JP 2013505078A JP 2013505078 A JP2013505078 A JP 2013505078A JP 5841997 B2 JP5841997 B2 JP 5841997B2
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cell
protein
cells
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JP2013523181A5 (enExample
JP2013523181A (ja
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ドミトリー・マルコフ
ネイサン・ゼンサー
デボラ・バッサー
ファン・ジャン
ホンギ・ジャン
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Sigma Aldrich Co LLC
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    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5041—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects involving analysis of members of signalling pathways
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
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    • C07K2319/00—Fusion polypeptide
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    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus

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JP2013505078A 2010-04-13 2011-04-13 内因性にタグ付けされたタンパク質を生成するための方法 Active JP5841997B2 (ja)

Applications Claiming Priority (15)

Application Number Priority Date Filing Date Title
US32370210P 2010-04-13 2010-04-13
US32369810P 2010-04-13 2010-04-13
US32371910P 2010-04-13 2010-04-13
US61/323,698 2010-04-13
US61/323,719 2010-04-13
US61/323,702 2010-04-13
US36701710P 2010-07-23 2010-07-23
US61/367,017 2010-07-23
US39066810P 2010-10-07 2010-10-07
US61/390,668 2010-10-07
US40885610P 2010-11-01 2010-11-01
US61/408,856 2010-11-01
US201161431957P 2011-01-12 2011-01-12
US61/431,957 2011-01-12
PCT/US2011/032218 WO2011130346A1 (en) 2010-04-13 2011-04-13 Methods for generating endogenously tagged protein

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JP2013523181A JP2013523181A (ja) 2013-06-17
JP2013523181A5 JP2013523181A5 (enExample) 2014-05-08
JP5841997B2 true JP5841997B2 (ja) 2016-01-13

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JP2013505077A Active JP5841996B2 (ja) 2010-04-13 2011-04-13 異種タンパク質を発現するための内因性プロモーターの使用
JP2013505078A Active JP5841997B2 (ja) 2010-04-13 2011-04-13 内因性にタグ付けされたタンパク質を生成するための方法

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US (5) US20130059362A1 (enExample)
EP (2) EP2558574B1 (enExample)
JP (2) JP5841996B2 (enExample)
KR (2) KR20130054955A (enExample)
CN (3) CN107805278A (enExample)
BR (1) BR112012026379A2 (enExample)
CA (2) CA2795636C (enExample)
DK (1) DK2558575T3 (enExample)
ES (1) ES2565216T3 (enExample)
WO (3) WO2011130343A1 (enExample)

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JP7233545B2 (ja) * 2018-12-30 2023-03-06 エフ． ホフマン－ラ ロシュ アーゲー 標的タンパク質への検出可能なタグのＣＲＩＳＰＲ／Ｃａｓ制御組み込みに基づく細胞の選択方法
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EP2558575B1 (en) 2015-12-16
WO2011130345A1 (en) 2011-10-20
KR20130055588A (ko) 2013-05-28
KR20130054955A (ko) 2013-05-27
US20130065310A1 (en) 2013-03-14
EP2558574A1 (en) 2013-02-20
CN102959078B (zh) 2016-01-20
EP2558575A1 (en) 2013-02-20
CN102959078A (zh) 2013-03-06
DK2558575T3 (en) 2016-03-21
JP2013523181A (ja) 2013-06-17
CA2795643A1 (en) 2011-10-20
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US20130059362A1 (en) 2013-03-07
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ES2565216T3 (es) 2016-04-01
WO2011130346A1 (en) 2011-10-20
US20180134759A1 (en) 2018-05-17
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