JP5841996B2 - 異種タンパク質を発現するための内因性プロモーターの使用 - Google Patents

異種タンパク質を発現するための内因性プロモーターの使用 Download PDF

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JP5841996B2
JP5841996B2 JP2013505077A JP2013505077A JP5841996B2 JP 5841996 B2 JP5841996 B2 JP 5841996B2 JP 2013505077 A JP2013505077 A JP 2013505077A JP 2013505077 A JP2013505077 A JP 2013505077A JP 5841996 B2 JP5841996 B2 JP 5841996B2
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sequence
protein
cell
heterologous protein
endogenous
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JP2013523180A5 (enExample
JP2013523180A (ja
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グレッグ・デイビス
ドミトリー・マルコフ
ネイサン・ゼンサー
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Sigma Aldrich Co LLC
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    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5035—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on sub-cellular localization
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
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    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5041—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects involving analysis of members of signalling pathways
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    • G01—MEASURING; TESTING
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    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
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    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C—CHEMISTRY; METALLURGY
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    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/72—Fusion polypeptide containing domain for protein-protein interaction containing SH2 domain
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    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus

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JP2013505077A 2010-04-13 2011-04-13 異種タンパク質を発現するための内因性プロモーターの使用 Active JP5841996B2 (ja)

Applications Claiming Priority (15)

Application Number Priority Date Filing Date Title
US32371910P 2010-04-13 2010-04-13
US32370210P 2010-04-13 2010-04-13
US32369810P 2010-04-13 2010-04-13
US61/323,702 2010-04-13
US61/323,698 2010-04-13
US61/323,719 2010-04-13
US36701710P 2010-07-23 2010-07-23
US61/367,017 2010-07-23
US39066810P 2010-10-07 2010-10-07
US61/390,668 2010-10-07
US40885610P 2010-11-01 2010-11-01
US61/408,856 2010-11-01
US201161431957P 2011-01-12 2011-01-12
US61/431,957 2011-01-12
PCT/US2011/032216 WO2011130345A1 (en) 2010-04-13 2011-04-13 Use of endogenous promoters to express heterologous proteins

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JP2013523180A JP2013523180A (ja) 2013-06-17
JP2013523180A5 JP2013523180A5 (enExample) 2014-05-08
JP5841996B2 true JP5841996B2 (ja) 2016-01-13

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JP2013505078A Active JP5841997B2 (ja) 2010-04-13 2011-04-13 内因性にタグ付けされたタンパク質を生成するための方法

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US (5) US20130059362A1 (enExample)
EP (2) EP2558574B1 (enExample)
JP (2) JP5841996B2 (enExample)
KR (2) KR20130054955A (enExample)
CN (3) CN107805278A (enExample)
BR (1) BR112012026379A2 (enExample)
CA (2) CA2795636C (enExample)
DK (1) DK2558575T3 (enExample)
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EP2558575B1 (en) 2015-12-16
WO2011130345A1 (en) 2011-10-20
KR20130055588A (ko) 2013-05-28
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US20130065310A1 (en) 2013-03-14
EP2558574A1 (en) 2013-02-20
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WO2011130346A1 (en) 2011-10-20
US20180134759A1 (en) 2018-05-17
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