JP5829808B2 - 組換えエラスターゼタンパク質ならびにその製造方法および使用 - Google Patents

組換えエラスターゼタンパク質ならびにその製造方法および使用 Download PDF

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JP5829808B2
JP5829808B2 JP2010537075A JP2010537075A JP5829808B2 JP 5829808 B2 JP5829808 B2 JP 5829808B2 JP 2010537075 A JP2010537075 A JP 2010537075A JP 2010537075 A JP2010537075 A JP 2010537075A JP 5829808 B2 JP5829808 B2 JP 5829808B2
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elastase
protein
sequence
mature
seq
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Japanese (ja)
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JP2011505164A (ja
JP2011505164A5 (OSRAM
Inventor
フラナノ，エフ．ニコラス
ブランド，キンバリー
ディー． ウォン，マルコ
ディー． ウォン，マルコ
シー． ディン，ビー
シー． ディン，ビー
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プロテオン セラピューティクス，インコーポレイテッド
プロテオン セラピューティクス，インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6448—Elastases, e.g. pancreatic elastase (3.4.21.36); leukocyte elastase (3.4.31.37)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A61K38/482—Serine endopeptidases (3.4.21)
    • A61K38/486—Elastase (3.4.21.36 or 3.4.21.37)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/19—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles lyophilised, i.e. freeze-dried, solutions or dispersions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21036—Pancreatic elastase (3.4.21.36)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21037—Leukocyte elastase (3.4.21.37), i.e. neutrophil-elastase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Cardiology (AREA)
  • Vascular Medicine (AREA)
  • Urology & Nephrology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2010537075A 2007-12-04 2008-12-04 組換えエラスターゼタンパク質ならびにその製造方法および使用 Expired - Fee Related JP5829808B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US99231907P 2007-12-04 2007-12-04
US60/992,319 2007-12-04
US12/327,809 US8501449B2 (en) 2007-12-04 2008-12-03 Recombinant elastase proteins and methods of manufacturing and use thereof
US12/327,809 2008-12-03
PCT/US2008/085559 WO2009079220A2 (en) 2007-12-04 2008-12-04 Recombinant elastase proteins and methods of manufacturing and use thereof

Related Child Applications (1)

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JP2015094887A Division JP6084653B2 (ja) 2007-12-04 2015-05-07 組換えエラスターゼタンパク質ならびにその製造方法および使用

Publications (3)

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JP2011505164A JP2011505164A (ja) 2011-02-24
JP2011505164A5 JP2011505164A5 (OSRAM) 2012-01-26
JP5829808B2 true JP5829808B2 (ja) 2015-12-09

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JP2010537075A Expired - Fee Related JP5829808B2 (ja) 2007-12-04 2008-12-04 組換えエラスターゼタンパク質ならびにその製造方法および使用
JP2015094887A Expired - Fee Related JP6084653B2 (ja) 2007-12-04 2015-05-07 組換えエラスターゼタンパク質ならびにその製造方法および使用
JP2017011160A Pending JP2017104114A (ja) 2007-12-04 2017-01-25 組換えエラスターゼタンパク質ならびにその製造方法および使用

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JP2017011160A Pending JP2017104114A (ja) 2007-12-04 2017-01-25 組換えエラスターゼタンパク質ならびにその製造方法および使用

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US (14) US8501449B2 (OSRAM)
EP (3) EP3176262A1 (OSRAM)
JP (3) JP5829808B2 (OSRAM)
KR (3) KR101732190B1 (OSRAM)
CN (4) CN104711243B (OSRAM)
AR (1) AR069584A1 (OSRAM)
AU (1) AU2008338743B2 (OSRAM)
BR (1) BRPI0819977A2 (OSRAM)
CA (1) CA2707051C (OSRAM)
CY (2) CY1114724T1 (OSRAM)
DK (2) DK2666854T3 (OSRAM)
ES (2) ES2627066T3 (OSRAM)
HR (2) HRP20131135T1 (OSRAM)
HU (1) HUE032739T2 (OSRAM)
IL (2) IL206157A (OSRAM)
LT (1) LT2666854T (OSRAM)
MX (2) MX341317B (OSRAM)
NZ (2) NZ585866A (OSRAM)
PL (2) PL2229440T3 (OSRAM)
PT (2) PT2229440E (OSRAM)
RS (2) RS55982B1 (OSRAM)
RU (1) RU2611200C2 (OSRAM)
SI (2) SI2229440T1 (OSRAM)
TW (2) TWI619726B (OSRAM)
WO (1) WO2009079220A2 (OSRAM)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8501449B2 (en) * 2007-12-04 2013-08-06 Proteon Therapeutics, Inc. Recombinant elastase proteins and methods of manufacturing and use thereof
CN102250863B (zh) * 2011-04-29 2012-12-05 天津金斯坦生物科技有限公司 基因重组人中性粒细胞弹性蛋白酶及其制备方法和该弹性蛋白酶的医药用途
US10138294B2 (en) * 2013-03-15 2018-11-27 Alderbio Holdings Llc Temperature shift for high yield expression of polypeptides in yeast and other transformed cells
US10202630B2 (en) 2013-03-15 2019-02-12 Alderbio Holdings Llc Temperature shift for high yield expression of polypeptides in yeast and other transformed cells
WO2018165594A1 (en) * 2017-03-10 2018-09-13 Bolt Threads, Inc. Compositions and methods for producing high secreted yields of recombinant proteins
ES3055526T3 (en) * 2017-06-15 2026-02-12 Univ Chicago Compositions for treating cancer
EP4065167A4 (en) * 2019-11-26 2024-03-27 Children's Hospital Medical Center CELA-1 INHIBITION FOR THE TREATMENT OF LUNG DISEASE
WO2021146233A1 (en) * 2020-01-14 2021-07-22 Basf Se Method for producing recombinant protein in yeast cells
CN116157513A (zh) * 2020-08-18 2023-05-23 昂希莱斯制药公司 经修饰的猪胰腺弹性蛋白酶蛋白
AU2021329330A1 (en) * 2020-08-18 2023-02-02 Onchilles Pharma, Inc. Modified serine protease proproteins

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2508408A (en) 1943-10-11 1950-05-23 Sidney H Liebson Averaging indicator
US2546308A (en) 1948-12-29 1951-03-27 Kahler John Floor marking device
US5212068A (en) * 1985-04-05 1993-05-18 Sankyo Company Limited Human pancreatic elastase
ATE141646T1 (de) 1986-04-09 1996-09-15 Genzyme Corp Genetisch transformierte tiere, die ein gewünschtes protein in milch absondern
JPH0673456B2 (ja) 1986-04-26 1994-09-21 三共株式会社 ヒト・膵臓エラスタ−ゼ▲ｉ▼
US5162205A (en) 1986-04-26 1992-11-10 Sankyo Company, Limited Human pancreatic elastase I
US4873316A (en) 1987-06-23 1989-10-10 Biogen, Inc. Isolation of exogenous recombinant proteins from the milk of transgenic mammals
US5543302A (en) 1988-05-27 1996-08-06 Solvay Enzymes, Inc. Proteases of altered stability to autolytic degradation
DK105489D0 (da) 1989-03-03 1989-03-03 Novo Nordisk As Polypeptid
ATE174058T1 (de) 1989-11-06 1998-12-15 Cell Genesys Inc Herstellung von proteinen mittels homologer rekombination
GB8927722D0 (en) 1989-12-07 1990-02-07 British Bio Technology Proteins and nucleic acids
US5272071A (en) 1989-12-22 1993-12-21 Applied Research Systems Ars Holding N.V. Method for the modification of the expression characteristics of an endogenous gene of a given cell line
NZ236819A (en) 1990-02-03 1993-07-27 Max Planck Gesellschaft Enzymatic cleavage of fusion proteins; fusion proteins; recombinant dna and pharmaceutical compositions
US5503333A (en) 1993-06-23 1996-04-02 Laventure; David Duster extension tube system with dust collecting region and tube cleaner and method of using the same
ATE226642T1 (de) 1994-07-07 2002-11-15 Tno Modifizierte proenzyme als substrate für proteolische enzyme
US5955072A (en) 1994-07-13 1999-09-21 Sankyo Company, Limited Expression systems utilizing autolyzing fusion proteins and a reducing polypeptide
US7531325B2 (en) 1997-04-25 2009-05-12 Sembiosys Genetics Inc. Method for cleavage of fusion proteins
US20030207402A1 (en) 1997-08-22 2003-11-06 Erhard Kopetzki Autocatalytically activatable zymogenic precursors of proteases and their use
GB9817909D0 (en) 1998-08-17 1998-10-14 Zeneca Ltd DNA constructs
GB9908458D0 (en) 1999-04-13 1999-06-09 Queen Mary & Westfield College Enzyme
AU4970900A (en) 1999-05-05 2000-11-21 Genentech Inc. Elastase variants and substrates
US7001892B1 (en) * 1999-06-11 2006-02-21 Purdue Research Foundation Pharmaceutical materials and methods for their preparation and use
DK1220830T3 (da) * 1999-09-24 2007-07-16 Proteon Therapeutics Inc Anvendelse af elastase til åbning af obstruerede arterier og vener
US7063838B1 (en) 1999-09-24 2006-06-20 Proteon Therapeutics Llc Methods for treating an artery or vein in a human subject
US7351549B2 (en) 2000-01-24 2008-04-01 Polymun Scientific Immunbiologische Forschung Gmbh Method for the manufacture of recombinant trypsin
US20010024789A1 (en) 2000-02-24 2001-09-27 Markus Kurz Methods for generating catalytic proteins
US20040142325A1 (en) * 2001-09-14 2004-07-22 Liat Mintz Methods and systems for annotating biomolecular sequences
US20060189941A1 (en) 2002-01-22 2006-08-24 Mercator Medsystems, Inc. Methods and kits for volumetric distribution of pharmaceutical agents via the vascular adventitia and microcirculation
WO2004073504A2 (en) 2003-02-20 2004-09-02 Proteon Therapeutics, Llc Methods for the treatment and prevention of diseases of biological conduits
US7571008B2 (en) 2003-08-18 2009-08-04 Medtronic, Inc. System and apparatus for remote activation of implantable medical devices
JP2008503712A (ja) * 2004-06-21 2008-02-07 ガラパゴス・ナムローゼ・フェンノートシャップ 骨関節炎治療の方法及び手段
US20090196865A1 (en) 2004-09-22 2009-08-06 Proteon Therapeutics, Inc. Methods for the treatment and prevention of diseases of biological conduits
RU2299070C2 (ru) * 2005-02-08 2007-05-20 Открытое акционерное общество "Химико-фармацевтический комбинат "АКРИХИН" (ОАО "АКРИХИН") Фармацевтическая композиция для лечения заболевания вен и способ ее получения
US20060193824A1 (en) 2005-02-28 2006-08-31 Rubin Bruce K Methods for the treatment of infectious and inflammatory airway diseases
CN1769424A (zh) * 2005-09-20 2006-05-10 浙江大学 一种芽孢杆菌菌株及其用途
NZ596269A (en) * 2006-07-05 2012-12-21 Catalyst Biosciences Inc Protease screening methods and proteases identified thereby
US8501449B2 (en) * 2007-12-04 2013-08-06 Proteon Therapeutics, Inc. Recombinant elastase proteins and methods of manufacturing and use thereof
WO2009124293A1 (en) * 2008-04-03 2009-10-08 Hudson Alpha Institute For Biotechnology Amplicon rescue multiplex polymerase chain reaction for amplificaton of multiple targets

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US8501449B2 (en) 2013-08-06
TW200938219A (en) 2009-09-16
RU2611200C2 (ru) 2017-02-21
US20110008315A1 (en) 2011-01-13
LT2666854T (lt) 2017-06-12
PL2229440T3 (pl) 2014-07-31
JP6084653B2 (ja) 2017-02-22
CN103952388A (zh) 2014-07-30
EP2229440B9 (en) 2013-11-13
EP3176262A1 (en) 2017-06-07
MX341317B (es) 2016-08-15
ES2436783T3 (es) 2014-01-07
RS55982B1 (sr) 2017-09-29
PT2666854T (pt) 2017-06-28
JP2011505164A (ja) 2011-02-24
US20150166974A1 (en) 2015-06-18
SI2666854T1 (sl) 2017-06-30
US10308924B2 (en) 2019-06-04
US20190010476A1 (en) 2019-01-10
TW201643188A (zh) 2016-12-16
KR20160095184A (ko) 2016-08-10
US20160319267A1 (en) 2016-11-03
ES2627066T3 (es) 2017-07-26
US20130336956A1 (en) 2013-12-19
AU2008338743A1 (en) 2009-06-25
WO2009079220A3 (en) 2009-08-06
NZ585866A (en) 2012-09-28
US9057060B2 (en) 2015-06-16
TWI577385B (zh) 2017-04-11
PT2229440E (pt) 2013-11-11
HUE032739T2 (en) 2017-10-30
CY1118930T1 (el) 2018-01-10
CA2707051C (en) 2019-05-21
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JP2017104114A (ja) 2017-06-15
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US20180208918A1 (en) 2018-07-26
EP2666854B1 (en) 2017-03-15
AU2008338743B2 (en) 2015-02-19
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IL206157A0 (en) 2010-12-30
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US20110081705A1 (en) 2011-04-07
US20190062718A1 (en) 2019-02-28
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US20160243205A1 (en) 2016-08-25
RS53083B (sr) 2014-06-30
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PL2666854T3 (pl) 2017-08-31
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WO2009079220A2 (en) 2009-06-25
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US20090162343A1 (en) 2009-06-25
KR101732190B1 (ko) 2017-05-04
AR069584A1 (es) 2010-02-03
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RU2010127232A (ru) 2012-01-10
US20180155703A1 (en) 2018-06-07
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