JP5824205B2 - 変異グルコース脱水素酵素 - Google Patents

変異グルコース脱水素酵素 Download PDF

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Publication number
JP5824205B2
JP5824205B2 JP2010240426A JP2010240426A JP5824205B2 JP 5824205 B2 JP5824205 B2 JP 5824205B2 JP 2010240426 A JP2010240426 A JP 2010240426A JP 2010240426 A JP2010240426 A JP 2010240426A JP 5824205 B2 JP5824205 B2 JP 5824205B2
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Prior art keywords
amino acid
glucose dehydrogenase
mutant
seq
subunit
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JP2010240426A
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Japanese (ja)
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JP2012090563A (ja
JP2012090563A5 (enExample
Inventor
早出　広司
広司 早出
勝博 小嶋
勝博 小嶋
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Arkray Inc
Bioengineering Laboratories LLC
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Arkray Inc
Bioengineering Laboratories LLC
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Priority to JP2010240426A priority Critical patent/JP5824205B2/ja
Priority to US13/282,243 priority patent/US20120107903A1/en
Priority to EP11186943.4A priority patent/EP2447358B1/en
Priority to CN201110404251.2A priority patent/CN102453702B/zh
Publication of JP2012090563A publication Critical patent/JP2012090563A/ja
Publication of JP2012090563A5 publication Critical patent/JP2012090563A5/ja
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Publication of JP5824205B2 publication Critical patent/JP5824205B2/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Emergency Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2010240426A 2010-10-27 2010-10-27 変異グルコース脱水素酵素 Active JP5824205B2 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2010240426A JP5824205B2 (ja) 2010-10-27 2010-10-27 変異グルコース脱水素酵素
US13/282,243 US20120107903A1 (en) 2010-10-27 2011-10-26 Mutant Glucose Dehydrogenase
EP11186943.4A EP2447358B1 (en) 2010-10-27 2011-10-27 Mutant glucose dehydrogenase
CN201110404251.2A CN102453702B (zh) 2010-10-27 2011-10-27 变异葡萄糖脱氢酶

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2010240426A JP5824205B2 (ja) 2010-10-27 2010-10-27 変異グルコース脱水素酵素

Publications (3)

Publication Number Publication Date
JP2012090563A JP2012090563A (ja) 2012-05-17
JP2012090563A5 JP2012090563A5 (enExample) 2013-11-21
JP5824205B2 true JP5824205B2 (ja) 2015-11-25

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ID=44862726

Family Applications (1)

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JP2010240426A Active JP5824205B2 (ja) 2010-10-27 2010-10-27 変異グルコース脱水素酵素

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US (1) US20120107903A1 (enExample)
EP (1) EP2447358B1 (enExample)
JP (1) JP5824205B2 (enExample)
CN (1) CN102453702B (enExample)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107532152A (zh) * 2014-12-04 2018-01-02 智慧酶生物医药有限公司 用于测量血糖水平的组合物和方法
WO2016087937A2 (en) * 2014-12-04 2016-06-09 Smartzyme Innovation Ltd. Compositions and methods for measuring blood glucose levels
CN108368489A (zh) * 2015-07-23 2018-08-03 智慧酶生物医药有限公司 用于测量血液葡萄糖水平的组合物和方法
JP6783108B2 (ja) * 2015-10-15 2020-11-11 アークレイ株式会社 酵素電極
EP3156791A1 (en) * 2015-10-15 2017-04-19 ARKRAY, Inc. Enzyme electrode
CN105331590B (zh) * 2015-10-29 2020-02-28 英科隆生物技术（杭州）有限公司 葡萄糖特异性改善的可溶性吡咯喹啉醌依赖性葡萄糖脱氢酶突变体、聚核苷酸及其应用
JP6856344B2 (ja) * 2015-10-29 2021-04-07 アークレイ株式会社 変異グルコース脱水素酵素およびその利用
EP3249050B1 (en) 2016-05-23 2019-01-23 ARKRAY, Inc. Enzyme electrode and biosensor using the same
JP7339723B2 (ja) * 2017-07-04 2023-09-06 アークレイ株式会社 変異型シトクロムタンパク質およびその利用
CN109425643B (zh) 2017-08-25 2022-10-25 爱科来株式会社 基于酶电化学阻抗测量法的新型生物传感技术
CN108486027A (zh) * 2018-04-04 2018-09-04 江南大学 一种fad为辅基的葡萄糖脱氢酶的生产、纯化方法
CN110514704B (zh) 2018-05-22 2024-02-13 爱科来株式会社 新型生物传感方法
US11293921B2 (en) 2018-10-03 2022-04-05 Arkray, Inc. Direct electron transfer-type oxidoreductase-modified molecular recognition element
EP4067483A1 (en) 2021-03-29 2022-10-05 The University of North Carolina at Chapel Hill Mutant glucose dehydrogenase having improved thermal stability, and use thereof
US20220306996A1 (en) * 2021-03-29 2022-09-29 The University Of North Carolina At Chapel Hill Mutant Glucose Dehydrogenase Having Improved Thermal Stability, and Use Thereof
CN113186175B (zh) * 2021-06-04 2023-08-04 翌圣生物科技(上海)股份有限公司 突变型Taq DNA聚合酶、编码DNA序列、重组载体、重组表达细胞及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4107386B2 (ja) 2000-10-31 2008-06-25 広司 早出 新規グルコース脱水素酵素及び該脱水素酵素の製造方法
WO2003091430A1 (fr) 2002-04-26 2003-11-06 Koji Sode SOUS-UNITE ss DE LA GLUCOSE DESHYDROGENASE ET ADN LA CODANT
AU2003246245B2 (en) * 2002-07-04 2008-09-18 Ultizyme International Ltd. Glucose dehydrogenase
US7803592B2 (en) * 2004-04-23 2010-09-28 Arkray, Inc. Mutant glucose dehydrogenase
ATE520771T1 (de) * 2005-06-20 2011-09-15 Arkray Inc Mutierte glucose-dehydrogenase

Also Published As

Publication number Publication date
EP2447358A1 (en) 2012-05-02
CN102453702A (zh) 2012-05-16
JP2012090563A (ja) 2012-05-17
EP2447358B1 (en) 2015-09-23
CN102453702B (zh) 2016-02-17
US20120107903A1 (en) 2012-05-03

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