JP5802389B2 - 合成ポリマーを用いた遺伝子サイレンシングに活性な核酸を送達する手段 - Google Patents

合成ポリマーを用いた遺伝子サイレンシングに活性な核酸を送達する手段 Download PDF

Info

Publication number
JP5802389B2
JP5802389B2 JP2010537590A JP2010537590A JP5802389B2 JP 5802389 B2 JP5802389 B2 JP 5802389B2 JP 2010537590 A JP2010537590 A JP 2010537590A JP 2010537590 A JP2010537590 A JP 2010537590A JP 5802389 B2 JP5802389 B2 JP 5802389B2
Authority
JP
Japan
Prior art keywords
sirna
composition
polyamine
tyrosine
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2010537590A
Other languages
English (en)
Japanese (ja)
Other versions
JP2011505826A5 (https=
JP2011505826A (ja
Inventor
アブデナジ アディブ
アブデナジ アディブ
パトリック エルバッハー
パトリック エルバッハー
ファブリス ストック
ファブリス ストック
ナディア ハフディ
ナディア ハフディ
Original Assignee
ポリプラス−トランスフェクション
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ポリプラス−トランスフェクション filed Critical ポリプラス−トランスフェクション
Publication of JP2011505826A publication Critical patent/JP2011505826A/ja
Publication of JP2011505826A5 publication Critical patent/JP2011505826A5/ja
Application granted granted Critical
Publication of JP5802389B2 publication Critical patent/JP5802389B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0008—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition
    • A61K48/0025—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition wherein the non-active part clearly interacts with the delivered nucleic acid
    • A61K48/0041—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition wherein the non-active part clearly interacts with the delivered nucleic acid the non-active part being polymeric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/30—Special therapeutic applications
    • C12N2320/32—Special delivery means, e.g. tissue-specific

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Polyamides (AREA)
JP2010537590A 2007-12-13 2008-12-12 合成ポリマーを用いた遺伝子サイレンシングに活性な核酸を送達する手段 Active JP5802389B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US1335807P 2007-12-13 2007-12-13
US61/013,358 2007-12-13
PCT/IB2008/055256 WO2009074970A2 (en) 2007-12-13 2008-12-12 Means for delivery of nucleic acids active for gene silencing using synthetic polymers

Publications (3)

Publication Number Publication Date
JP2011505826A JP2011505826A (ja) 2011-03-03
JP2011505826A5 JP2011505826A5 (https=) 2011-12-15
JP5802389B2 true JP5802389B2 (ja) 2015-10-28

Family

ID=40561891

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2010537590A Active JP5802389B2 (ja) 2007-12-13 2008-12-12 合成ポリマーを用いた遺伝子サイレンシングに活性な核酸を送達する手段

Country Status (8)

Country Link
US (1) US20100297756A1 (https=)
EP (1) EP2229445B1 (https=)
JP (1) JP5802389B2 (https=)
KR (1) KR101590652B1 (https=)
CN (1) CN101952434B (https=)
CA (1) CA2708354C (https=)
IL (1) IL206310A (https=)
WO (1) WO2009074970A2 (https=)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8945927B2 (en) 2010-03-29 2015-02-03 Universite De Strasbourg Polymers for delivering molecules of interest
KR101223483B1 (ko) * 2010-09-10 2013-01-17 한국과학기술연구원 전하결합 및 생분해성 공유결합으로 동시에 연결된 고분자―ｓｉＲＮＡ 나노입자 전달체
KR101497668B1 (ko) 2012-09-28 2015-03-03 한양대학교 산학협력단 전신 순환을 위한 짧은 간섭 rna 유전자 전달체
WO2014051318A2 (ko) * 2012-09-28 2014-04-03 한양대학교 산학협력단 전신 순환을 위한 짧은 간섭 rna 유전자 전달체
WO2014053245A1 (en) 2012-10-05 2014-04-10 Lipocalyx Gmbh Hydroxylated polyamine derivatives as transfection reagents
AU2013329889B2 (en) 2012-10-08 2018-03-08 Biontech Delivery Technologies Gmbh Carboxylated polyamine derivatives as transfection reagents
CN103087314B (zh) * 2013-02-01 2015-02-18 同济大学 生物可降解聚氨酯基因转染试剂的制备方法
CN107805643B (zh) * 2017-09-15 2020-12-08 四川大学 靶向抑制沙门氏菌耐药外排泵基因acrA的siRNA-DNA纳米系统及其制备方法
BR112022002143A2 (pt) * 2019-08-05 2022-04-19 Polyplus Transfection Composição adequada para transfectar uma molécula de ácido nucleico em uma célula, métodos para transfecção in vitro ou ex vivo de células vivas e para a produção e usos in vitro ou ex vivo da composição
DE102020114183B4 (de) 2020-05-27 2022-07-14 Universität Leipzig Dezernat Forschung und Transfer Mittel zur transfektion von nukleinsäuren in zellen
CN112195193A (zh) * 2020-09-30 2021-01-08 上海交通大学 单链dna在基因转染中的应用
WO2023164285A1 (en) * 2022-02-28 2023-08-31 Northwestern University DISE-INDUCING sRNA-POLYPLEXES AND sRNA-LIPOPOLYPLEXES AND METHODS OF USING THE SAME TO TREAT CANCER
WO2026036019A1 (en) 2024-08-09 2026-02-12 Life Technologies Corporation Method of producing a recombinant protein in hek-293 cells

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7427394B2 (en) * 2000-10-10 2008-09-23 Massachusetts Institute Of Technology Biodegradable poly(β-amino esters) and uses thereof
US7601367B2 (en) * 2002-05-28 2009-10-13 Mirus Bio Llc Compositions and processes using siRNA, amphipathic compounds and polycations
CN1720257A (zh) * 2002-08-06 2006-01-11 因特拉迪格姆公司 通过导入干扰rna在体内下调靶基因表达的方法
BRPI0407107A (pt) * 2003-01-31 2006-01-24 Novartis Ag Infra-regulação de gene alvo com complexos de oligoribonucleotìdeo de filamento único e polìmero de polietilenoimina (pei)
CA2523785A1 (en) * 2003-05-09 2004-11-25 University Of Pittsburgh Of The Commonwealth System Of Higher Education Small interfering rna libraries and methods of synthesis and use
ES2559828T3 (es) * 2003-07-16 2016-02-16 Protiva Biotherapeutics Inc. ARN de interferencia encapsulado en lípidos
US7842673B2 (en) * 2003-12-17 2010-11-30 The Trustees Of Columbia University In The City Of New York Delivery of DNA or RNA via gap junctions from host cells to target cells and a cell-based delivery system for antisense or siRNA
EP1841867A1 (en) * 2005-01-24 2007-10-10 Avaris AB COMPLEX CONTAINING SiRNA, ShRNA OR ANTISENSE MOLECULE AND FUNCTIONAL ENTITY, FOR IMPROVED SPECIFICITY AND DELIVERY
US20100310670A1 (en) * 2005-09-02 2010-12-09 Takeda Pharmaceutical Company Limited Sustained-release microsphere containing short chain deoxyribonucleic acid or short chain ribonucleic acid and method of producing the same
KR100863405B1 (ko) * 2005-09-12 2008-10-14 주식회사 대웅 암 진단 마커 및 이의 이용
JP2007119396A (ja) * 2005-10-28 2007-05-17 Hosokawa Funtai Gijutsu Kenkyusho:Kk 核酸化合物封入ナノ粒子を含む経肺投与用医薬製剤
GB0613753D0 (en) * 2006-07-11 2006-08-23 Norwegian Radium Hospital Res Method
CN101085356A (zh) * 2007-06-21 2007-12-12 中国科学院长春应用化学研究所 一种非病毒基因转染载体、其与质粒dna复合物颗粒及制备方法和用法

Also Published As

Publication number Publication date
CA2708354A1 (en) 2009-06-18
CN101952434A (zh) 2011-01-19
EP2229445A2 (en) 2010-09-22
IL206310A (en) 2013-07-31
US20100297756A1 (en) 2010-11-25
IL206310A0 (en) 2010-12-30
CA2708354C (en) 2018-12-11
KR20100105674A (ko) 2010-09-29
JP2011505826A (ja) 2011-03-03
WO2009074970A2 (en) 2009-06-18
EP2229445B1 (en) 2012-12-05
CN101952434B (zh) 2016-11-16
WO2009074970A3 (en) 2009-10-29
KR101590652B1 (ko) 2016-02-18

Similar Documents

Publication Publication Date Title
JP5802389B2 (ja) 合成ポリマーを用いた遺伝子サイレンシングに活性な核酸を送達する手段
CN108026527B (zh) 确定的多偶联寡核苷酸
Zhang et al. Cationic lipids and polymers mediated vectors for delivery of siRNA
US7101995B2 (en) Compositions and processes using siRNA, amphipathic compounds and polycations
CA2717778C (fr) Polymere derive de la polyethylenimine lineaire pour le transfert de gene
JP2018518186A5 (https=)
JP2011505826A5 (https=)
CN105452465A (zh) 鞘脂-聚烷基胺-寡核苷酸化合物
US20040019008A1 (en) Compositions and processes using siRNA, amphipathic compounds and polycations
WO2003040375A1 (en) COMPOSITIONS AND PROCESSES USING siRNA, AMPHIPATHIC COMPOUNDS AND POLYCATIONS
CA3179016A1 (en) Double-stranded oligonucleotide and composition for treating covid-19 containing same
US8945927B2 (en) Polymers for delivering molecules of interest
Grijalvo et al. Synthesis of oligonucleotides carrying amino lipid groups at the 3′-end for RNA interference studies
HK1149052B (en) Means for delivery of nucleic acids active for gene silencing using synthetic polymers
HK1149052A (en) Means for delivery of nucleic acids active for gene silencing using synthetic polymers
EP2395041A1 (en) Polymers for delivery of nucleic acids
FR3108330A1 (fr) Copolymere lineaire pour la transfection de genes
CN101384282B (zh) 制备转化细胞的方法
Katas Development of nanocarriers for siRNA based on cationic polymers

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20111026

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20111026

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20130827

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20131126

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20140708

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20141107

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20150107

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20150310

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150602

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20150804

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20150831

R150 Certificate of patent or registration of utility model

Ref document number: 5802389

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250