JP5785098B2 - 単一ポリマー相系を用いる分離方法 - Google Patents

単一ポリマー相系を用いる分離方法 Download PDF

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Publication number
JP5785098B2
JP5785098B2 JP2011545321A JP2011545321A JP5785098B2 JP 5785098 B2 JP5785098 B2 JP 5785098B2 JP 2011545321 A JP2011545321 A JP 2011545321A JP 2011545321 A JP2011545321 A JP 2011545321A JP 5785098 B2 JP5785098 B2 JP 5785098B2
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Japan
Prior art keywords
phase
polymer
target
eopo
mab
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JP2011545321A
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English (en)
Japanese (ja)
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JP2012514637A (ja
JP2012514637A5 (enExample
Inventor
アルスティネ，ヤメス ファン
アルスティネ，ヤメス ファン
シャナガール，ヤミル
フヨルス，ロルフ
ハル，マルティン
ニルセン，カミラ エストメール
ニルセン，カミラ エストメール
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Cytiva Sweden AB
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Amersham Bioscience AB
GE Healthcare Bio Sciences Corp
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Publication of JP2012514637A5 publication Critical patent/JP2012514637A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/04—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from milk
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2011545321A 2009-01-08 2010-01-07 単一ポリマー相系を用いる分離方法 Active JP5785098B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE0900014-2 2009-01-08
SE0900014 2009-01-08
PCT/SE2010/050008 WO2010080062A1 (en) 2009-01-08 2010-01-07 Separation method using single polymer phase systems

Publications (3)

Publication Number Publication Date
JP2012514637A JP2012514637A (ja) 2012-06-28
JP2012514637A5 JP2012514637A5 (enExample) 2013-02-21
JP5785098B2 true JP5785098B2 (ja) 2015-09-24

Family

ID=42316654

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2011545321A Active JP5785098B2 (ja) 2009-01-08 2010-01-07 単一ポリマー相系を用いる分離方法

Country Status (7)

Country Link
US (1) US9115181B2 (enExample)
EP (1) EP2373676B1 (enExample)
JP (1) JP5785098B2 (enExample)
CN (1) CN102272144B (enExample)
AU (1) AU2010203836B2 (enExample)
CA (1) CA2748807C (enExample)
WO (1) WO2010080062A1 (enExample)

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JP5947802B2 (ja) * 2010-11-05 2016-07-06 エフ・ホフマン−ラ・ロシュ・アクチェンゲゼルシャフト ミックスモードクロマトグラフィーによる抗体捕捉の最適化方法
EP2702077A2 (en) 2011-04-27 2014-03-05 AbbVie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
CN103797023B (zh) * 2011-09-16 2020-04-10 通用电气医疗集团生物工艺研发股份公司 通过序贯多元酸沉淀进行的血浆蛋白分级分离
WO2013158273A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Methods to modulate c-terminal lysine variant distribution
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
WO2013158279A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
EP2682168A1 (en) 2012-07-02 2014-01-08 Millipore Corporation Purification of biological molecules
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
CA2905010A1 (en) 2013-03-12 2014-09-18 Abbvie Inc. Human antibodies that bind human tnf-alpha and methods of preparing the same
US10023608B1 (en) 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
WO2014151878A2 (en) 2013-03-14 2014-09-25 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
US9828599B2 (en) 2013-09-18 2017-11-28 General Electric Company Methods of selecting binding-elements and uses thereof
US10359419B2 (en) 2013-10-02 2019-07-23 General Electric Company Methods for detection of target using affinity binding
US20150093839A1 (en) 2013-10-02 2015-04-02 General Electric Company Methods and systems for detection of target and applications thereof
US9598667B2 (en) 2013-10-04 2017-03-21 Abbvie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
KR101745455B1 (ko) * 2015-03-24 2017-06-09 포항공과대학교 산학협력단 수용액 이상계를 이용한 세포 밖 소포체의 다단계 정제방법
KR101761680B1 (ko) * 2015-03-31 2017-08-23 포항공과대학교 산학협력단 수용액 이상계를 이용한 세포 밖 소포체의 분리방법
CA3055565A1 (en) 2017-03-08 2018-09-13 The Regents Of The University Of Michigan Analyte detection
CN114839374A (zh) 2017-03-28 2022-08-02 相达生物科技国际有限公司 用于在液体活检中准确诊断疾病靶向生物标记的方法
WO2018222972A1 (en) 2017-06-01 2018-12-06 Yin To Chiu Phase separation behavior modifying agents for aqueous two-phase separation within porous material
US10450345B2 (en) 2017-06-09 2019-10-22 General Electric Company Method of isolation of polypeptide-aptamer-polymer conjugates
US11609232B2 (en) 2017-09-01 2023-03-21 Phase Diagnostics, Inc. Method and device of using aqueous two-phase systems (ATPS) for enhancing diagnostics for sexually transmitted infections
ES2964100T3 (es) 2017-09-18 2024-04-04 Phase Scient International Ltd Método de utilización de un sistema acuoso bifásico para el aislamiento, purificación y/o concentración de fragmentos cortos de ácidos nucleicos
US12043826B2 (en) 2018-01-19 2024-07-23 Phase Scientific International, Ltd. Spontaneous nucleic acid purification and concentration in a single step
EP3740588A4 (en) 2018-01-19 2021-10-20 Phase Scientific International, Ltd. METHOD OF ISOLATION AND PURIFICATION OF NUCLEIC ACIDS USING A SOLID-LIQUID PHASE SYSTEM
WO2019143943A2 (en) 2018-01-19 2019-07-25 Yin To Chiu Composition and method for concentration and enrichment of nucleic acids
US20210015350A1 (en) * 2018-03-30 2021-01-21 Blaze Bioscience, Inc. Systems and methods for simultaneous near-infrared light and visible light imaging
WO2020036869A1 (en) 2018-08-16 2020-02-20 Emd Millipore Corporation Closed bioprocessing device
BR112022018784A2 (pt) 2020-03-20 2023-01-31 Phase Scient International Ltd Composições e métodos para extração de ácido ribonucleico
CN112321704A (zh) * 2020-11-20 2021-02-05 华东理工大学 利用液态热响应型聚合物eo20po80分离纯化单克隆抗体
WO2025193698A1 (en) * 2024-03-11 2025-09-18 Michigan Technological University Purification of viral particles with two stage aqueous two-phase extraction

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US6454950B1 (en) * 1998-12-30 2002-09-24 Amersham Pharmacia Biotech Ab Separation method utilizing liquid-liquid partition
US6544788B2 (en) * 2001-02-15 2003-04-08 Vijay Singh Disposable perfusion bioreactor for cell culture
AU2006327328B2 (en) * 2005-12-22 2011-08-04 Ge Healthcare Bio-Sciences Ab Preparation of biomolecules
EP2155773A4 (en) * 2007-06-19 2012-10-24 Ge Healthcare Bio Sciences Ab SEPARATION METHOD USING MULTIPHASE POLYMER SYSTEMS
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Also Published As

Publication number Publication date
CN102272144A (zh) 2011-12-07
AU2010203836B2 (en) 2015-05-28
CA2748807C (en) 2019-01-29
JP2012514637A (ja) 2012-06-28
US9115181B2 (en) 2015-08-25
US20120010390A1 (en) 2012-01-12
EP2373676A1 (en) 2011-10-12
WO2010080062A1 (en) 2010-07-15
CN102272144B (zh) 2014-09-17
CA2748807A1 (en) 2010-07-15
AU2010203836A1 (en) 2011-07-07
EP2373676A4 (en) 2013-02-27
EP2373676B1 (en) 2017-04-19

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