JP2012514637A5 - - Google Patents
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- JP2012514637A5 JP2012514637A5 JP2011545321A JP2011545321A JP2012514637A5 JP 2012514637 A5 JP2012514637 A5 JP 2012514637A5 JP 2011545321 A JP2011545321 A JP 2011545321A JP 2011545321 A JP2011545321 A JP 2011545321A JP 2012514637 A5 JP2012514637 A5 JP 2012514637A5
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- JP
- Japan
- Prior art keywords
- phase
- polymer
- target
- chromatography
- concentrated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000000034 method Methods 0.000 claims 22
- 229920000642 polymer Polymers 0.000 claims 15
- 150000001875 compounds Chemical class 0.000 claims 6
- RVGRUAULSDPKGF-UHFFFAOYSA-N Poloxamer Chemical compound C1CO1.CC1CO1 RVGRUAULSDPKGF-UHFFFAOYSA-N 0.000 claims 4
- 239000007788 liquid Substances 0.000 claims 4
- 239000003446 ligand Substances 0.000 claims 3
- 150000003839 salts Chemical class 0.000 claims 3
- 229920000896 Ethulose Polymers 0.000 claims 2
- 239000001859 Ethyl hydroxyethyl cellulose Substances 0.000 claims 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 2
- 238000001042 affinity chromatography Methods 0.000 claims 2
- 230000002051 biphasic effect Effects 0.000 claims 2
- 229920001400 block copolymer Polymers 0.000 claims 2
- 235000019326 ethyl hydroxyethyl cellulose Nutrition 0.000 claims 2
- 238000000855 fermentation Methods 0.000 claims 2
- 230000004151 fermentation Effects 0.000 claims 2
- 238000004255 ion exchange chromatography Methods 0.000 claims 2
- 238000004811 liquid chromatography Methods 0.000 claims 2
- 229920001223 polyethylene glycol Polymers 0.000 claims 2
- 102000004169 proteins and genes Human genes 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- 239000002028 Biomass Substances 0.000 claims 1
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 claims 1
- 241001274216 Naso Species 0.000 claims 1
- 239000007864 aqueous solution Substances 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 238000005352 clarification Methods 0.000 claims 1
- 230000000295 complement effect Effects 0.000 claims 1
- 229920001577 copolymer Polymers 0.000 claims 1
- 125000001301 ethoxy group Chemical group [H]C([H])([H])C([H])([H])O* 0.000 claims 1
- 239000012634 fragment Substances 0.000 claims 1
- 150000004676 glycans Chemical class 0.000 claims 1
- 229920001477 hydrophilic polymer Polymers 0.000 claims 1
- 230000002209 hydrophobic effect Effects 0.000 claims 1
- 238000004191 hydrophobic interaction chromatography Methods 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 230000007935 neutral effect Effects 0.000 claims 1
- 239000002245 particle Substances 0.000 claims 1
- 238000005192 partition Methods 0.000 claims 1
- 230000005501 phase interface Effects 0.000 claims 1
- 229920001983 poloxamer Polymers 0.000 claims 1
- 229920000570 polyether Polymers 0.000 claims 1
- 229920001282 polysaccharide Polymers 0.000 claims 1
- 239000005017 polysaccharide Substances 0.000 claims 1
- 239000001508 potassium citrate Substances 0.000 claims 1
- 229960002635 potassium citrate Drugs 0.000 claims 1
- QEEAPRPFLLJWCF-UHFFFAOYSA-K potassium citrate (anhydrous) Chemical compound [K+].[K+].[K+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O QEEAPRPFLLJWCF-UHFFFAOYSA-K 0.000 claims 1
- 235000011082 potassium citrates Nutrition 0.000 claims 1
- 238000004064 recycling Methods 0.000 claims 1
- 239000011734 sodium Substances 0.000 claims 1
- 239000001632 sodium acetate Substances 0.000 claims 1
- 235000017281 sodium acetate Nutrition 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
- 239000001509 sodium citrate Substances 0.000 claims 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims 1
- PVGBHEUCHKGFQP-UHFFFAOYSA-N sodium;n-[5-amino-2-(4-aminophenyl)sulfonylphenyl]sulfonylacetamide Chemical compound [Na+].CC(=O)NS(=O)(=O)C1=CC(N)=CC=C1S(=O)(=O)C1=CC=C(N)C=C1 PVGBHEUCHKGFQP-UHFFFAOYSA-N 0.000 claims 1
- 238000000638 solvent extraction Methods 0.000 claims 1
- 229920000208 temperature-responsive polymer Polymers 0.000 claims 1
- 238000010977 unit operation Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE0900014-2 | 2009-01-08 | ||
| SE0900014 | 2009-01-08 | ||
| PCT/SE2010/050008 WO2010080062A1 (en) | 2009-01-08 | 2010-01-07 | Separation method using single polymer phase systems |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2012514637A JP2012514637A (ja) | 2012-06-28 |
| JP2012514637A5 true JP2012514637A5 (enExample) | 2013-02-21 |
| JP5785098B2 JP5785098B2 (ja) | 2015-09-24 |
Family
ID=42316654
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011545321A Active JP5785098B2 (ja) | 2009-01-08 | 2010-01-07 | 単一ポリマー相系を用いる分離方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US9115181B2 (enExample) |
| EP (1) | EP2373676B1 (enExample) |
| JP (1) | JP5785098B2 (enExample) |
| CN (1) | CN102272144B (enExample) |
| AU (1) | AU2010203836B2 (enExample) |
| CA (1) | CA2748807C (enExample) |
| WO (1) | WO2010080062A1 (enExample) |
Families Citing this family (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2500413A1 (en) | 2006-09-13 | 2012-09-19 | Abbott Laboratories | Cell culture improvements |
| US8911964B2 (en) | 2006-09-13 | 2014-12-16 | Abbvie Inc. | Fed-batch method of making human anti-TNF-alpha antibody |
| NZ592097A (en) | 2008-10-20 | 2013-01-25 | Abbott Lab | Viral inactivation during purification of il-12 and il-18 antibodies |
| KR20110091678A (ko) | 2008-10-20 | 2011-08-12 | 아보트 러보러터리즈 | 단백질 ａ 친화성 크로마토그래피를 이용한 항체의 분리 및 정제 |
| JP5947802B2 (ja) * | 2010-11-05 | 2016-07-06 | エフ・ホフマン−ラ・ロシュ・アクチェンゲゼルシャフト | ミックスモードクロマトグラフィーによる抗体捕捉の最適化方法 |
| EP2702077A2 (en) | 2011-04-27 | 2014-03-05 | AbbVie Inc. | Methods for controlling the galactosylation profile of recombinantly-expressed proteins |
| CN103797023B (zh) * | 2011-09-16 | 2020-04-10 | 通用电气医疗集团生物工艺研发股份公司 | 通过序贯多元酸沉淀进行的血浆蛋白分级分离 |
| WO2013158273A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Methods to modulate c-terminal lysine variant distribution |
| US9067990B2 (en) | 2013-03-14 | 2015-06-30 | Abbvie, Inc. | Protein purification using displacement chromatography |
| WO2013158279A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Protein purification methods to reduce acidic species |
| EP2682168A1 (en) | 2012-07-02 | 2014-01-08 | Millipore Corporation | Purification of biological molecules |
| US9512214B2 (en) | 2012-09-02 | 2016-12-06 | Abbvie, Inc. | Methods to control protein heterogeneity |
| CA2905010A1 (en) | 2013-03-12 | 2014-09-18 | Abbvie Inc. | Human antibodies that bind human tnf-alpha and methods of preparing the same |
| US10023608B1 (en) | 2013-03-13 | 2018-07-17 | Amgen Inc. | Protein purification methods to remove impurities |
| WO2014151878A2 (en) | 2013-03-14 | 2014-09-25 | Abbvie Inc. | Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides |
| US9017687B1 (en) | 2013-10-18 | 2015-04-28 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same using displacement chromatography |
| US9828599B2 (en) | 2013-09-18 | 2017-11-28 | General Electric Company | Methods of selecting binding-elements and uses thereof |
| US10359419B2 (en) | 2013-10-02 | 2019-07-23 | General Electric Company | Methods for detection of target using affinity binding |
| US20150093839A1 (en) | 2013-10-02 | 2015-04-02 | General Electric Company | Methods and systems for detection of target and applications thereof |
| US9598667B2 (en) | 2013-10-04 | 2017-03-21 | Abbvie Inc. | Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins |
| US9181337B2 (en) | 2013-10-18 | 2015-11-10 | Abbvie, Inc. | Modulated lysine variant species compositions and methods for producing and using the same |
| US9085618B2 (en) | 2013-10-18 | 2015-07-21 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same |
| US20150139988A1 (en) | 2013-11-15 | 2015-05-21 | Abbvie, Inc. | Glycoengineered binding protein compositions |
| KR101745455B1 (ko) * | 2015-03-24 | 2017-06-09 | 포항공과대학교 산학협력단 | 수용액 이상계를 이용한 세포 밖 소포체의 다단계 정제방법 |
| KR101761680B1 (ko) * | 2015-03-31 | 2017-08-23 | 포항공과대학교 산학협력단 | 수용액 이상계를 이용한 세포 밖 소포체의 분리방법 |
| CA3055565A1 (en) | 2017-03-08 | 2018-09-13 | The Regents Of The University Of Michigan | Analyte detection |
| CN114839374A (zh) | 2017-03-28 | 2022-08-02 | 相达生物科技国际有限公司 | 用于在液体活检中准确诊断疾病靶向生物标记的方法 |
| WO2018222972A1 (en) | 2017-06-01 | 2018-12-06 | Yin To Chiu | Phase separation behavior modifying agents for aqueous two-phase separation within porous material |
| US10450345B2 (en) | 2017-06-09 | 2019-10-22 | General Electric Company | Method of isolation of polypeptide-aptamer-polymer conjugates |
| US11609232B2 (en) | 2017-09-01 | 2023-03-21 | Phase Diagnostics, Inc. | Method and device of using aqueous two-phase systems (ATPS) for enhancing diagnostics for sexually transmitted infections |
| ES2964100T3 (es) | 2017-09-18 | 2024-04-04 | Phase Scient International Ltd | Método de utilización de un sistema acuoso bifásico para el aislamiento, purificación y/o concentración de fragmentos cortos de ácidos nucleicos |
| US12043826B2 (en) | 2018-01-19 | 2024-07-23 | Phase Scientific International, Ltd. | Spontaneous nucleic acid purification and concentration in a single step |
| EP3740588A4 (en) | 2018-01-19 | 2021-10-20 | Phase Scientific International, Ltd. | METHOD OF ISOLATION AND PURIFICATION OF NUCLEIC ACIDS USING A SOLID-LIQUID PHASE SYSTEM |
| WO2019143943A2 (en) | 2018-01-19 | 2019-07-25 | Yin To Chiu | Composition and method for concentration and enrichment of nucleic acids |
| US20210015350A1 (en) * | 2018-03-30 | 2021-01-21 | Blaze Bioscience, Inc. | Systems and methods for simultaneous near-infrared light and visible light imaging |
| WO2020036869A1 (en) | 2018-08-16 | 2020-02-20 | Emd Millipore Corporation | Closed bioprocessing device |
| BR112022018784A2 (pt) | 2020-03-20 | 2023-01-31 | Phase Scient International Ltd | Composições e métodos para extração de ácido ribonucleico |
| CN112321704A (zh) * | 2020-11-20 | 2021-02-05 | 华东理工大学 | 利用液态热响应型聚合物eo20po80分离纯化单克隆抗体 |
| WO2025193698A1 (en) * | 2024-03-11 | 2025-09-18 | Michigan Technological University | Purification of viral particles with two stage aqueous two-phase extraction |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5429746A (en) * | 1994-02-22 | 1995-07-04 | Smith Kline Beecham Corporation | Antibody purification |
| US6454950B1 (en) * | 1998-12-30 | 2002-09-24 | Amersham Pharmacia Biotech Ab | Separation method utilizing liquid-liquid partition |
| US6544788B2 (en) * | 2001-02-15 | 2003-04-08 | Vijay Singh | Disposable perfusion bioreactor for cell culture |
| AU2006327328B2 (en) * | 2005-12-22 | 2011-08-04 | Ge Healthcare Bio-Sciences Ab | Preparation of biomolecules |
| EP2155773A4 (en) * | 2007-06-19 | 2012-10-24 | Ge Healthcare Bio Sciences Ab | SEPARATION METHOD USING MULTIPHASE POLYMER SYSTEMS |
| WO2008156410A1 (en) | 2007-06-19 | 2008-12-24 | Ge Healthcare Bio-Sciences Ab | Polymer two phase system and use thereof |
-
2010
- 2010-01-07 EP EP10729364.9A patent/EP2373676B1/en active Active
- 2010-01-07 AU AU2010203836A patent/AU2010203836B2/en active Active
- 2010-01-07 US US13/143,560 patent/US9115181B2/en active Active
- 2010-01-07 WO PCT/SE2010/050008 patent/WO2010080062A1/en not_active Ceased
- 2010-01-07 JP JP2011545321A patent/JP5785098B2/ja active Active
- 2010-01-07 CN CN201080004357.7A patent/CN102272144B/zh active Active
- 2010-01-07 CA CA2748807A patent/CA2748807C/en active Active
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