CN102272144B - 采用单一聚合物相系统的分离方法 - Google Patents

采用单一聚合物相系统的分离方法 Download PDF

Info

Publication number
CN102272144B
CN102272144B CN201080004357.7A CN201080004357A CN102272144B CN 102272144 B CN102272144 B CN 102272144B CN 201080004357 A CN201080004357 A CN 201080004357A CN 102272144 B CN102272144 B CN 102272144B
Authority
CN
China
Prior art keywords
phase
polymer
target
eopo
mab
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201080004357.7A
Other languages
English (en)
Chinese (zh)
Other versions
CN102272144A (zh
Inventor
J·范阿尔斯廷
J·沙纳加尔
R·霍尔思
M·哈尔
C·埃斯特默尼尔森
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Bioprocess R&D AB
Original Assignee
GE Healthcare Bio Sciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GE Healthcare Bio Sciences Corp filed Critical GE Healthcare Bio Sciences Corp
Publication of CN102272144A publication Critical patent/CN102272144A/zh
Application granted granted Critical
Publication of CN102272144B publication Critical patent/CN102272144B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/04—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from milk
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201080004357.7A 2009-01-08 2010-01-07 采用单一聚合物相系统的分离方法 Active CN102272144B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE0900014-2 2009-01-08
SE0900014 2009-01-08
PCT/SE2010/050008 WO2010080062A1 (en) 2009-01-08 2010-01-07 Separation method using single polymer phase systems

Publications (2)

Publication Number Publication Date
CN102272144A CN102272144A (zh) 2011-12-07
CN102272144B true CN102272144B (zh) 2014-09-17

Family

ID=42316654

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201080004357.7A Active CN102272144B (zh) 2009-01-08 2010-01-07 采用单一聚合物相系统的分离方法

Country Status (7)

Country Link
US (1) US9115181B2 (enExample)
EP (1) EP2373676B1 (enExample)
JP (1) JP5785098B2 (enExample)
CN (1) CN102272144B (enExample)
AU (1) AU2010203836B2 (enExample)
CA (1) CA2748807C (enExample)
WO (1) WO2010080062A1 (enExample)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2500413A1 (en) 2006-09-13 2012-09-19 Abbott Laboratories Cell culture improvements
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
NZ592097A (en) 2008-10-20 2013-01-25 Abbott Lab Viral inactivation during purification of il-12 and il-18 antibodies
KR20110091678A (ko) 2008-10-20 2011-08-12 아보트 러보러터리즈 단백질 ａ 친화성 크로마토그래피를 이용한 항체의 분리 및 정제
JP5947802B2 (ja) * 2010-11-05 2016-07-06 エフ・ホフマン−ラ・ロシュ・アクチェンゲゼルシャフト ミックスモードクロマトグラフィーによる抗体捕捉の最適化方法
EP2702077A2 (en) 2011-04-27 2014-03-05 AbbVie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
CN103797023B (zh) * 2011-09-16 2020-04-10 通用电气医疗集团生物工艺研发股份公司 通过序贯多元酸沉淀进行的血浆蛋白分级分离
WO2013158273A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Methods to modulate c-terminal lysine variant distribution
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
WO2013158279A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
EP2682168A1 (en) 2012-07-02 2014-01-08 Millipore Corporation Purification of biological molecules
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
CA2905010A1 (en) 2013-03-12 2014-09-18 Abbvie Inc. Human antibodies that bind human tnf-alpha and methods of preparing the same
US10023608B1 (en) 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
WO2014151878A2 (en) 2013-03-14 2014-09-25 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
US9828599B2 (en) 2013-09-18 2017-11-28 General Electric Company Methods of selecting binding-elements and uses thereof
US10359419B2 (en) 2013-10-02 2019-07-23 General Electric Company Methods for detection of target using affinity binding
US20150093839A1 (en) 2013-10-02 2015-04-02 General Electric Company Methods and systems for detection of target and applications thereof
US9598667B2 (en) 2013-10-04 2017-03-21 Abbvie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
KR101745455B1 (ko) * 2015-03-24 2017-06-09 포항공과대학교 산학협력단 수용액 이상계를 이용한 세포 밖 소포체의 다단계 정제방법
KR101761680B1 (ko) * 2015-03-31 2017-08-23 포항공과대학교 산학협력단 수용액 이상계를 이용한 세포 밖 소포체의 분리방법
CA3055565A1 (en) 2017-03-08 2018-09-13 The Regents Of The University Of Michigan Analyte detection
CN114839374A (zh) 2017-03-28 2022-08-02 相达生物科技国际有限公司 用于在液体活检中准确诊断疾病靶向生物标记的方法
WO2018222972A1 (en) 2017-06-01 2018-12-06 Yin To Chiu Phase separation behavior modifying agents for aqueous two-phase separation within porous material
US10450345B2 (en) 2017-06-09 2019-10-22 General Electric Company Method of isolation of polypeptide-aptamer-polymer conjugates
US11609232B2 (en) 2017-09-01 2023-03-21 Phase Diagnostics, Inc. Method and device of using aqueous two-phase systems (ATPS) for enhancing diagnostics for sexually transmitted infections
ES2964100T3 (es) 2017-09-18 2024-04-04 Phase Scient International Ltd Método de utilización de un sistema acuoso bifásico para el aislamiento, purificación y/o concentración de fragmentos cortos de ácidos nucleicos
US12043826B2 (en) 2018-01-19 2024-07-23 Phase Scientific International, Ltd. Spontaneous nucleic acid purification and concentration in a single step
EP3740588A4 (en) 2018-01-19 2021-10-20 Phase Scientific International, Ltd. METHOD OF ISOLATION AND PURIFICATION OF NUCLEIC ACIDS USING A SOLID-LIQUID PHASE SYSTEM
WO2019143943A2 (en) 2018-01-19 2019-07-25 Yin To Chiu Composition and method for concentration and enrichment of nucleic acids
US20210015350A1 (en) * 2018-03-30 2021-01-21 Blaze Bioscience, Inc. Systems and methods for simultaneous near-infrared light and visible light imaging
WO2020036869A1 (en) 2018-08-16 2020-02-20 Emd Millipore Corporation Closed bioprocessing device
BR112022018784A2 (pt) 2020-03-20 2023-01-31 Phase Scient International Ltd Composições e métodos para extração de ácido ribonucleico
CN112321704A (zh) * 2020-11-20 2021-02-05 华东理工大学 利用液态热响应型聚合物eo20po80分离纯化单克隆抗体
WO2025193698A1 (en) * 2024-03-11 2025-09-18 Michigan Technological University Purification of viral particles with two stage aqueous two-phase extraction

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008156410A1 (en) * 2007-06-19 2008-12-24 Ge Healthcare Bio-Sciences Ab Polymer two phase system and use thereof
WO2008156409A1 (en) * 2007-06-19 2008-12-24 Ge Healthcare Bio-Sciences Ab Separation method using polymer multi phase systems

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5429746A (en) * 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
US6454950B1 (en) * 1998-12-30 2002-09-24 Amersham Pharmacia Biotech Ab Separation method utilizing liquid-liquid partition
US6544788B2 (en) * 2001-02-15 2003-04-08 Vijay Singh Disposable perfusion bioreactor for cell culture
AU2006327328B2 (en) * 2005-12-22 2011-08-04 Ge Healthcare Bio-Sciences Ab Preparation of biomolecules

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008156410A1 (en) * 2007-06-19 2008-12-24 Ge Healthcare Bio-Sciences Ab Polymer two phase system and use thereof
WO2008156409A1 (en) * 2007-06-19 2008-12-24 Ge Healthcare Bio-Sciences Ab Separation method using polymer multi phase systems

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Application of temperature-induced phase partitioning at ambient temperature for enzyme purification;Patricia A. Alred等;《Journal of Chromatography A》;19941231;第659卷(第2期);摘要 *
Integrated process for the purification of antibodies combining aqueous two-phase extraction, hydrophobic interaction chromatography and size-exclusion chromatography;A.M. Azevedo等;《Journal of Chromatography A》;20081231;第1231卷(第2期);摘要 *
Purification of recombinant apolipoprotein A-1Milano expressed in Escherichia coli using aqueous two-phase extraction followed by temperature-induced phase separation;Josefine Persson等;《Journal of Chromatography B》;19981231;第711卷(第1-2期);摘要 *
Thermoseparating water/Polymer System: A Novel One-Polymer Aqueous Two-Phase System for Protein Purification;Hans-Olof Johansson等;《Biotechnology and bioengineering》;19991231;第66卷(第4期);摘要，第248页右栏第1-3行，第250页第2、3段，第253页第2段，第256页倒数第1段倒数第3-4行，图2，第249页第3段 *

Also Published As

Publication number Publication date
CN102272144A (zh) 2011-12-07
AU2010203836B2 (en) 2015-05-28
CA2748807C (en) 2019-01-29
JP2012514637A (ja) 2012-06-28
US9115181B2 (en) 2015-08-25
JP5785098B2 (ja) 2015-09-24
US20120010390A1 (en) 2012-01-12
EP2373676A1 (en) 2011-10-12
WO2010080062A1 (en) 2010-07-15
CA2748807A1 (en) 2010-07-15
AU2010203836A1 (en) 2011-07-07
EP2373676A4 (en) 2013-02-27
EP2373676B1 (en) 2017-04-19

Similar Documents

Publication Publication Date Title
CN102272144B (zh) 采用单一聚合物相系统的分离方法
Marichal-Gallardo et al. Steric exclusion chromatography for purification of cell culture-derived influenza A virus using regenerated cellulose membranes and polyethylene glycol
US20120022239A1 (en) Precipitation of biomolecules with negatively charged polymers
AU2012309202B2 (en) Plasma protein fractionation by sequential polyacid precipitation
US20110257378A1 (en) Aqueous two phase extraction augmented precipitation process for purification of therapeutic proteins
US9695216B2 (en) Materials and methods for removing endotoxins from protein preparations
JP2015533378A (ja) ２段階接線流限外濾過を使用するポリペプチドの精製
CN105008384A (zh) 在高电导率下在非离子性有机聚合物的存在下的蛋白质纯化
JP2016507588A (ja) 非イオン性有機ポリマーおよび電気陽性表面の存在下でのタンパク質精製
EP3508493B1 (en) Protein purification method using activated carbon
Van Alstine et al. Alternative separation methods: Flocculation and precipitation
CN119895024A (zh) 从灌注培养物收获产物的方法
Iyer et al. Flow-through purification of viruses-a novel approach to vaccine purification
US7157562B1 (en) Bioprocess for the production of recombinant anti-botulinum toxin antibody
WO2010043700A1 (en) Clarification process at higher cell density
Ghosh Bioseparations using integrated membrane processes
EA046702B1 (ru) Способы очистки биспецифических антител при помощи катионообменной хроматографии с использованием промывки с высоким содержанием соли
Labisch et al. Lab-Scale Tools for Advanced LV Downstream Processing
HK1208872B (zh) 使用双级切线流超滤纯化多肽

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
C41 Transfer of patent application or patent right or utility model
TR01 Transfer of patent right

Effective date of registration: 20160816

Address after: uppsala

Patentee after: GE HEALTHCARE BIO-SCIENCES AB

Address before: uppsala

Patentee before: GE Health Bio-science Co., Ltd.

CP01 Change in the name or title of a patent holder

Address after: uppsala

Patentee after: Situofan Biotechnology R & D Co., Ltd

Address before: uppsala

Patentee before: GE HEALTHCARE BIOPROCESS R&D AB

CP01 Change in the name or title of a patent holder