JP5714023B2 - 結合の挙動の分析のための方法及びシステム - Google Patents
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- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
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- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/55—Specular reflectivity
- G01N21/552—Attenuated total reflection
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G01N33/557—Immunoassay; Biospecific binding assay; Materials therefor using kinetic measurement, i.e. time rate of progress of an antigen-antibody interaction
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Description
拡散又は質量の移動が制限されず擬一次速度式に従う、分析物Aと表面に結合した(固定された)捕捉分子、すなわちリガンドBとの間の可逆反応を仮定する。
この相互作用モデル(通常ラングミュア型と呼ばれる)は、分析物(A)が一価かつ均質であり、リガンド(B)が均質であり、全ての結合事象が独立であるということを仮定し、実際に、大部分の場合に適用可能である。
ここで、式(4)に従って、dR/dtが結合した分析物の濃度Rに対してプロットされると、傾きは−(kaC+kd)であり、縦軸の切片はkaCRmaxである。体積濃度Cが既知であり、飽和応答Rmaxが求められている(例えば、大量の過剰な分析物で表面を飽和させることで)場合、会合速度定数ka及び解離速度定数kdを計算することができる。しかし、より便利な方法は、好ましくはコンピュータプログラムによる、積分された関数(5)のフィッティング、又は微分方程式(4)の数値計算及びフィッティングである。
アフィニティは、会合定数KA=ka/kdにより、又は、解離定数(平衡定数とも呼ばれる)KD=kd/kaにより表現される。
kdReq=kaC(Rmax−Req) (9)
が得られ、Reqは平衡状態における検出器の応答である。ka/kd=KAなので、式(9)に代入して整理すると、
反応速度及びアフィニティのデータを分析するためのソフトウェアは、市販されている。したがって、例えば、BIACORE(登録商標)装置により生成された、反応速度及びアフィニティのデータは、通常、(スウェーデン・ウプサラのBiacore ABにより提供される)専用のBIA評価ソフトウェアにより評価される。このソフトウェアは、数値積分を用いて、微分形の速度方程式を計算し、非線形回帰を用いて、フィッティングが最もよく合う変数の値を見つけることによって反応速度及びアフィニティのパラメータをフィッティングして、残差平方和を最小にする。
BIACORE(登録商標)装置のような、バイオセンサーを用いる相互作用スクリーニングアッセイに対して、評価に用いられる相互作用パラメータは、センサグラム中の決められた点(報告点)の応答から読み取られることが多い。しかし、センサグラムは、データの相互作用及び品質についてより多くの情報を含み、この情報は、含まれる全てのセンサグラムを評価することを正当なものにする。大量のデータセット(数百又は数千のセンサグラム)に対しては、これは非常に面倒な手順になりうる。
A:リガンドが固定化されたセンサー表面を用意する段階(図3の符号10)と、
B:センサー表面を複数の分析物と接触させる段階(図3の符号20)と、
C:複数の分析物の各々についてセンサー応答曲線を記録する段階(図3の符号30)と、
D:各々のセンサー応答曲線から、相互作用パラメータを抽出する段階(図3の符号40)と、
E:2つ以上の結合挙動基準に従って各々のセンサー応答曲線を評価する段階(図3の符号50)と、
F:相互作用パラメータを図式的に表示する段階(図3の符号60)であって、ある結合挙動基準を満たす応答曲線に関連する相互作用パラメータを、別の結合挙動基準を満たすか或いはいずれの結合挙動基準も満たさない応答曲線に関する相互作用パラメータとグラフィカルに識別できる、段階と
を含む。
(i)1つ以上の検知表面と、1つ以上の検知表面において分子結合の相互作用を検出するための検出手段と、結合曲線を表す検出データを生成するための手段とを含む、バイオセンサーであって、各曲線が、時間に対する結合の相互作用の進行を表す、バイオセンサーと、
(ii)上記の方法の段階A〜Hを実行するための、データ処理手段とを、含む。
2 金属膜
3 リガンド
4 分析物
5 流路
6 単色のp偏光した光
7 光源
8 プリズム
9 境界
10 反射された光線
11 光検出ユニット
Claims (8)
- 複数の分析物とリガンドとの相互作用についての相互作用パラメータを、バイオセンサーを用いて評価するための方法であって、
A:前記リガンドが固定化されたセンサー表面を用意する段階と、
B:前記センサー表面を前記複数の分析物と接触させる段階と、
C:前記複数の分析物の各々についてセンサー応答曲線を記録する段階と、
D:各々のセンサー応答曲線から、相互作用パラメータを抽出する段階と、
E:1以上の結合挙動基準に従って各々のセンサー応答曲線を時間の関数として評価する段階と、
F:前記相互作用パラメータを図式的に表示する段階であって、ある結合挙動基準を満たす応答曲線に関連する相互作用パラメータを、別の結合挙動基準を満たすか或いはいずれの結合挙動基準も満たさない応答曲線に関する相互作用パラメータとグラフィカルに識別できる、段階と
を含む方法。 - 2以上の結合挙動基準を満たす応答曲線に関連する相互作用パラメータが、いずれの結合挙動基準も満たさないか或いは1つの結合挙動基準を満たす応答曲線に関連する相互作用パラメータとグラフィカルに識別できる、請求項1記載の方法。
- 1つの結合挙動基準が、前記記録された応答が分析物の接触時間の間に所定の率を超える率で増大することである、請求項1記載の方法。
- 1つの結合挙動基準が、前記分析物が前記センサー表面と接触した後で、前記記録された応答が所定の値を超えることである、請求項1記載の方法。
- 1つの結合挙動基準が、前記記録された最大の応答が、前記分析物の相互作用に対する所定の値を超えることである、請求項1記載の方法。
- 前記センサー表面が、各分析物の間に再生される、請求項1記載の方法。
- 分子結合相互作用を検出するための分析システムであって、
(i)1以上の検知表面と、前記1以上の検知表面での分子結合相互作用を検出するための検出手段と、結合曲線であって、各曲線が結合相互作用の経時的な進行を表す結合曲線を表す検出データを生成するための手段とを含むバイオセンサーと、
(ii)請求項1記載の段階A〜Fを実行するための、請求項1乃至請求項6のいずれか1項で定義されるデータ処理手段と
を含む分析システム。 - プログラムコード手段を含むコンピュータプログラムであって、前記プログラムがコンピュータ上で実行されると、前記プログラムコード手段が、請求項1乃至請求項6のいずれか1項に従って、分析物とリガンドとの相互作用についての1以上の相互作用パラメータを求める、コンピュータプログラム。
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PCT/SE2010/051311 WO2011065913A1 (en) | 2009-11-30 | 2010-11-29 | Method and system for binding behavior analysis |
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WO2012118433A1 (en) * | 2011-02-28 | 2012-09-07 | Ge Healthcare Bio-Sciences Ab | Screening method |
JP6540710B2 (ja) | 2013-11-19 | 2019-07-10 | ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ | 速い解離速度の相互作用カイネティクスの決定方法 |
EP3100048B1 (en) * | 2014-01-29 | 2021-05-05 | Cytiva Sweden AB | Method and system for interaction analysis |
GB201515070D0 (en) * | 2015-08-25 | 2015-10-07 | Ge Healthcare Bio Sciences Ab | Method for determining affinity of a biomolecule |
GB201517985D0 (en) * | 2015-10-12 | 2015-11-25 | Ge Healthcare Bio Sciences Ab | A method in a surface plasmon resonance biosensor system |
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US9063156B2 (en) * | 2009-06-12 | 2015-06-23 | Pacific Biosciences Of California, Inc. | Real-time analytical methods and systems |
ES2701832T3 (es) * | 2014-05-27 | 2019-02-26 | Academia Sinica | Dispositivo de detección, y sistema de detección y método de detección que usan el mismo |
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CN102667447A (zh) | 2012-09-12 |
EP2507619A4 (en) | 2013-08-28 |
US20120244638A1 (en) | 2012-09-27 |
US10151694B2 (en) | 2018-12-11 |
JP2013512432A (ja) | 2013-04-11 |
CN102667447B (zh) | 2016-03-23 |
EP2507619A1 (en) | 2012-10-10 |
WO2011065913A1 (en) | 2011-06-03 |
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