JP5663841B2 - 骨細胞への分化誘導培地及び方法 - Google Patents

骨細胞への分化誘導培地及び方法 Download PDF

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Publication number
JP5663841B2
JP5663841B2 JP2009038998A JP2009038998A JP5663841B2 JP 5663841 B2 JP5663841 B2 JP 5663841B2 JP 2009038998 A JP2009038998 A JP 2009038998A JP 2009038998 A JP2009038998 A JP 2009038998A JP 5663841 B2 JP5663841 B2 JP 5663841B2
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Japan
Prior art keywords
medium
differentiation
vitamin
cells
stem cells
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Expired - Fee Related
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JP2009038998A
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English (en)
Japanese (ja)
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JP2010193723A (ja
JP2010193723A5 (enExample
Inventor
佳之 堀田
佳之 堀田
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Fujirebio Inc
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Fujirebio Inc
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Application filed by Fujirebio Inc filed Critical Fujirebio Inc
Priority to JP2009038998A priority Critical patent/JP5663841B2/ja
Priority to EP10743811.1A priority patent/EP2399987B1/en
Priority to US13/202,713 priority patent/US9315777B2/en
Priority to PCT/JP2010/052455 priority patent/WO2010095685A1/ja
Priority to CA2753303A priority patent/CA2753303A1/en
Publication of JP2010193723A publication Critical patent/JP2010193723A/ja
Publication of JP2010193723A5 publication Critical patent/JP2010193723A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/42—Organic phosphate, e.g. beta glycerophosphate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Microbiology (AREA)
  • Rheumatology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2009038998A 2009-02-23 2009-02-23 骨細胞への分化誘導培地及び方法 Expired - Fee Related JP5663841B2 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2009038998A JP5663841B2 (ja) 2009-02-23 2009-02-23 骨細胞への分化誘導培地及び方法
EP10743811.1A EP2399987B1 (en) 2009-02-23 2010-02-18 Culture medium and method to induce differentiation into bone cells
US13/202,713 US9315777B2 (en) 2009-02-23 2010-02-18 Culture medium and method for inducing differentiation into bone cells
PCT/JP2010/052455 WO2010095685A1 (ja) 2009-02-23 2010-02-18 骨細胞への分化誘導培地及び方法
CA2753303A CA2753303A1 (en) 2009-02-23 2010-02-18 Culture medium and method for inducing differentiation into bone cells

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2009038998A JP5663841B2 (ja) 2009-02-23 2009-02-23 骨細胞への分化誘導培地及び方法

Publications (3)

Publication Number Publication Date
JP2010193723A JP2010193723A (ja) 2010-09-09
JP2010193723A5 JP2010193723A5 (enExample) 2012-04-05
JP5663841B2 true JP5663841B2 (ja) 2015-02-04

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Family Applications (1)

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JP2009038998A Expired - Fee Related JP5663841B2 (ja) 2009-02-23 2009-02-23 骨細胞への分化誘導培地及び方法

Country Status (5)

Country Link
US (1) US9315777B2 (enExample)
EP (1) EP2399987B1 (enExample)
JP (1) JP5663841B2 (enExample)
CA (1) CA2753303A1 (enExample)
WO (1) WO2010095685A1 (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150231209A1 (en) * 2012-09-13 2015-08-20 The Board Of Trustees Of The Leland Stanford Junior University Stimulation of ovarian follicle development and oocyte maturation
CN113025566A (zh) * 2020-12-30 2021-06-25 无锡市第九人民医院 一种内皮细胞成骨诱导分化培养基及制备方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2251746T3 (es) * 1996-12-06 2006-05-01 Osiris Therapeutics, Inc. Diferenciacion condrogenica mejorada de celulas precursoras mesenquimatosas humanas.
US6153582A (en) * 1998-11-05 2000-11-28 Bausch & Lomb Surgical, Inc. Defined serumfree medical solution for ophthalmology
WO2003104442A1 (en) * 2002-06-07 2003-12-18 Es Cell International Pte Ltd Methods of regulating differentiation in stem cells
EP1651756B1 (en) * 2003-08-01 2010-11-24 Two Cells Co., Ltd Scaffold-free self-organized 3d synthetic tissue
JP2006055106A (ja) 2004-08-23 2006-03-02 National Institute Of Advanced Industrial & Technology ヒト血清培地を用いるヒト骨髄由来間葉系幹細胞培養法
KR101107835B1 (ko) * 2006-01-13 2012-02-09 가부시키가이샤 투셀 동물 세포의 무혈청 배양에 사용하기 위한 배양 배지 첨가물, 키트 및 이의 용도
LV13634B (en) 2007-05-02 2007-12-20 Jp Biotechnology Sia Production of ethanol and feedstuffs
US20110008893A1 (en) * 2007-12-28 2011-01-13 Fujirebio Inc. Medium for mammalian somatic cells and additive therefor
JP5515319B2 (ja) * 2009-02-23 2014-06-11 富士レビオ株式会社 脂肪細胞への分化誘導培地及び方法

Also Published As

Publication number Publication date
WO2010095685A1 (ja) 2010-08-26
EP2399987B1 (en) 2015-11-04
JP2010193723A (ja) 2010-09-09
CA2753303A1 (en) 2010-08-26
EP2399987A4 (en) 2013-12-25
EP2399987A1 (en) 2011-12-28
US9315777B2 (en) 2016-04-19
US20110306132A1 (en) 2011-12-15

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