CA2753303A1 - Culture medium and method for inducing differentiation into bone cells - Google Patents

Culture medium and method for inducing differentiation into bone cells Download PDF

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Publication number
CA2753303A1
CA2753303A1 CA2753303A CA2753303A CA2753303A1 CA 2753303 A1 CA2753303 A1 CA 2753303A1 CA 2753303 A CA2753303 A CA 2753303A CA 2753303 A CA2753303 A CA 2753303A CA 2753303 A1 CA2753303 A1 CA 2753303A1
Authority
CA
Canada
Prior art keywords
cells
medium
differentiation
culture medium
serum
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2753303A
Other languages
English (en)
French (fr)
Inventor
Yoshiyuki Hotta
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujirebio Inc
Original Assignee
Fujirebio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fujirebio Inc filed Critical Fujirebio Inc
Publication of CA2753303A1 publication Critical patent/CA2753303A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/42—Organic phosphate, e.g. beta glycerophosphate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Microbiology (AREA)
  • Rheumatology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA2753303A 2009-02-23 2010-02-18 Culture medium and method for inducing differentiation into bone cells Abandoned CA2753303A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2009-038998 2009-02-23
JP2009038998A JP5663841B2 (ja) 2009-02-23 2009-02-23 骨細胞への分化誘導培地及び方法
PCT/JP2010/052455 WO2010095685A1 (ja) 2009-02-23 2010-02-18 骨細胞への分化誘導培地及び方法

Publications (1)

Publication Number Publication Date
CA2753303A1 true CA2753303A1 (en) 2010-08-26

Family

ID=42633965

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2753303A Abandoned CA2753303A1 (en) 2009-02-23 2010-02-18 Culture medium and method for inducing differentiation into bone cells

Country Status (5)

Country Link
US (1) US9315777B2 (enExample)
EP (1) EP2399987B1 (enExample)
JP (1) JP5663841B2 (enExample)
CA (1) CA2753303A1 (enExample)
WO (1) WO2010095685A1 (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150231209A1 (en) * 2012-09-13 2015-08-20 The Board Of Trustees Of The Leland Stanford Junior University Stimulation of ovarian follicle development and oocyte maturation
CN113025566A (zh) * 2020-12-30 2021-06-25 无锡市第九人民医院 一种内皮细胞成骨诱导分化培养基及制备方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2251746T3 (es) * 1996-12-06 2006-05-01 Osiris Therapeutics, Inc. Diferenciacion condrogenica mejorada de celulas precursoras mesenquimatosas humanas.
US6153582A (en) * 1998-11-05 2000-11-28 Bausch & Lomb Surgical, Inc. Defined serumfree medical solution for ophthalmology
WO2003104442A1 (en) * 2002-06-07 2003-12-18 Es Cell International Pte Ltd Methods of regulating differentiation in stem cells
EP1651756B1 (en) * 2003-08-01 2010-11-24 Two Cells Co., Ltd Scaffold-free self-organized 3d synthetic tissue
JP2006055106A (ja) 2004-08-23 2006-03-02 National Institute Of Advanced Industrial & Technology ヒト血清培地を用いるヒト骨髄由来間葉系幹細胞培養法
KR101107835B1 (ko) * 2006-01-13 2012-02-09 가부시키가이샤 투셀 동물 세포의 무혈청 배양에 사용하기 위한 배양 배지 첨가물, 키트 및 이의 용도
LV13634B (en) 2007-05-02 2007-12-20 Jp Biotechnology Sia Production of ethanol and feedstuffs
US20110008893A1 (en) * 2007-12-28 2011-01-13 Fujirebio Inc. Medium for mammalian somatic cells and additive therefor
JP5515319B2 (ja) * 2009-02-23 2014-06-11 富士レビオ株式会社 脂肪細胞への分化誘導培地及び方法

Also Published As

Publication number Publication date
WO2010095685A1 (ja) 2010-08-26
EP2399987B1 (en) 2015-11-04
JP2010193723A (ja) 2010-09-09
EP2399987A4 (en) 2013-12-25
JP5663841B2 (ja) 2015-02-04
EP2399987A1 (en) 2011-12-28
US9315777B2 (en) 2016-04-19
US20110306132A1 (en) 2011-12-15

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Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20141001

FZDE Discontinued

Effective date: 20180105