JP2010193723A5 - - Google Patents
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- Publication number
- JP2010193723A5 JP2010193723A5 JP2009038998A JP2009038998A JP2010193723A5 JP 2010193723 A5 JP2010193723 A5 JP 2010193723A5 JP 2009038998 A JP2009038998 A JP 2009038998A JP 2009038998 A JP2009038998 A JP 2009038998A JP 2010193723 A5 JP2010193723 A5 JP 2010193723A5
- Authority
- JP
- Japan
- Prior art keywords
- medium
- medium according
- stem cells
- somatic stem
- differentiation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000001988 somatic stem cell Anatomy 0.000 claims description 8
- 210000002449 bone cell Anatomy 0.000 claims description 7
- 239000001963 growth medium Substances 0.000 claims description 2
- 239000002609 medium Substances 0.000 claims 17
- 230000004069 differentiation Effects 0.000 claims 6
- 230000009762 endothelial cell differentiation Effects 0.000 claims 5
- 230000001939 inductive effect Effects 0.000 claims 5
- 108090000623 proteins and genes Proteins 0.000 claims 5
- 239000003446 ligand Substances 0.000 claims 4
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 claims 3
- 239000011669 selenium Substances 0.000 claims 3
- 229910052711 selenium Inorganic materials 0.000 claims 3
- WRGQSWVCFNIUNZ-GDCKJWNLSA-N 1-oleoyl-sn-glycerol 3-phosphate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@@H](O)COP(O)(O)=O WRGQSWVCFNIUNZ-GDCKJWNLSA-N 0.000 claims 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 claims 2
- DHCLVCXQIBBOPH-UHFFFAOYSA-N Glycerol 2-phosphate Chemical group OCC(CO)OP(O)(O)=O DHCLVCXQIBBOPH-UHFFFAOYSA-N 0.000 claims 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims 2
- 239000000654 additive Substances 0.000 claims 2
- 230000000996 additive effect Effects 0.000 claims 2
- AWUCVROLDVIAJX-UHFFFAOYSA-N alpha-glycerophosphate Natural products OCC(O)COP(O)(O)=O AWUCVROLDVIAJX-UHFFFAOYSA-N 0.000 claims 2
- 239000007640 basal medium Substances 0.000 claims 2
- 239000003795 chemical substances by application Substances 0.000 claims 2
- -1 sphingosine monophosphate Chemical class 0.000 claims 2
- WWUZIQQURGPMPG-UHFFFAOYSA-N (-)-D-erythro-Sphingosine Natural products CCCCCCCCCCCCCC=CC(O)C(N)CO WWUZIQQURGPMPG-UHFFFAOYSA-N 0.000 claims 1
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 claims 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims 1
- YJPIGAIKUZMOQA-UHFFFAOYSA-N Melatonin Natural products COC1=CC=C2N(C(C)=O)C=C(CCN)C2=C1 YJPIGAIKUZMOQA-UHFFFAOYSA-N 0.000 claims 1
- 239000012980 RPMI-1640 medium Substances 0.000 claims 1
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 claims 1
- 229930003268 Vitamin C Natural products 0.000 claims 1
- 229930003316 Vitamin D Natural products 0.000 claims 1
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 claims 1
- 229930003448 Vitamin K Natural products 0.000 claims 1
- 239000000556 agonist Substances 0.000 claims 1
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 claims 1
- 238000004113 cell culture Methods 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 229960003957 dexamethasone Drugs 0.000 claims 1
- UREBDLICKHMUKA-CXSFZGCWSA-N dexamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-CXSFZGCWSA-N 0.000 claims 1
- 235000013922 glutamic acid Nutrition 0.000 claims 1
- 239000004220 glutamic acid Substances 0.000 claims 1
- 239000000411 inducer Substances 0.000 claims 1
- 210000004962 mammalian cell Anatomy 0.000 claims 1
- DRLFMBDRBRZALE-UHFFFAOYSA-N melatonin Chemical compound COC1=CC=C2NC=C(CCNC(C)=O)C2=C1 DRLFMBDRBRZALE-UHFFFAOYSA-N 0.000 claims 1
- 229960003987 melatonin Drugs 0.000 claims 1
- 238000000034 method Methods 0.000 claims 1
- SHUZOJHMOBOZST-UHFFFAOYSA-N phylloquinone Natural products CC(C)CCCCC(C)CCC(C)CCCC(=CCC1=C(C)C(=O)c2ccccc2C1=O)C SHUZOJHMOBOZST-UHFFFAOYSA-N 0.000 claims 1
- 150000003839 salts Chemical class 0.000 claims 1
- 210000002966 serum Anatomy 0.000 claims 1
- 239000012679 serum free medium Substances 0.000 claims 1
- 235000019155 vitamin A Nutrition 0.000 claims 1
- 239000011719 vitamin A Substances 0.000 claims 1
- 235000019154 vitamin C Nutrition 0.000 claims 1
- 239000011718 vitamin C Substances 0.000 claims 1
- 235000019166 vitamin D Nutrition 0.000 claims 1
- 239000011710 vitamin D Substances 0.000 claims 1
- 150000003710 vitamin D derivatives Chemical class 0.000 claims 1
- 235000019168 vitamin K Nutrition 0.000 claims 1
- 239000011712 vitamin K Substances 0.000 claims 1
- 150000003721 vitamin K derivatives Chemical class 0.000 claims 1
- 229940045997 vitamin a Drugs 0.000 claims 1
- 229940046008 vitamin d Drugs 0.000 claims 1
- 229940046010 vitamin k Drugs 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
- 239000011701 zinc Substances 0.000 claims 1
- 229910052725 zinc Inorganic materials 0.000 claims 1
- 235000016804 zinc Nutrition 0.000 claims 1
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000002901 mesenchymal stem cell Anatomy 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2009038998A JP5663841B2 (ja) | 2009-02-23 | 2009-02-23 | 骨細胞への分化誘導培地及び方法 |
| US13/202,713 US9315777B2 (en) | 2009-02-23 | 2010-02-18 | Culture medium and method for inducing differentiation into bone cells |
| PCT/JP2010/052455 WO2010095685A1 (ja) | 2009-02-23 | 2010-02-18 | 骨細胞への分化誘導培地及び方法 |
| EP10743811.1A EP2399987B1 (en) | 2009-02-23 | 2010-02-18 | Culture medium and method to induce differentiation into bone cells |
| CA2753303A CA2753303A1 (en) | 2009-02-23 | 2010-02-18 | Culture medium and method for inducing differentiation into bone cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2009038998A JP5663841B2 (ja) | 2009-02-23 | 2009-02-23 | 骨細胞への分化誘導培地及び方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010193723A JP2010193723A (ja) | 2010-09-09 |
| JP2010193723A5 true JP2010193723A5 (enExample) | 2012-04-05 |
| JP5663841B2 JP5663841B2 (ja) | 2015-02-04 |
Family
ID=42633965
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009038998A Expired - Fee Related JP5663841B2 (ja) | 2009-02-23 | 2009-02-23 | 骨細胞への分化誘導培地及び方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US9315777B2 (enExample) |
| EP (1) | EP2399987B1 (enExample) |
| JP (1) | JP5663841B2 (enExample) |
| CA (1) | CA2753303A1 (enExample) |
| WO (1) | WO2010095685A1 (enExample) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150231209A1 (en) * | 2012-09-13 | 2015-08-20 | The Board Of Trustees Of The Leland Stanford Junior University | Stimulation of ovarian follicle development and oocyte maturation |
| CN113025566A (zh) * | 2020-12-30 | 2021-06-25 | 无锡市第九人民医院 | 一种内皮细胞成骨诱导分化培养基及制备方法 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU742638B2 (en) * | 1996-12-06 | 2002-01-10 | Mesoblast International Sarl | Improved chondrogenic differentiation of human mesenchymal stem cells |
| US6153582A (en) * | 1998-11-05 | 2000-11-28 | Bausch & Lomb Surgical, Inc. | Defined serumfree medical solution for ophthalmology |
| CA2488425A1 (en) | 2002-06-07 | 2003-12-18 | Es Cell International Pte Ltd | Methods of regulating differentiation in stem cells |
| JP4522994B2 (ja) * | 2003-08-01 | 2010-08-11 | 憲正 中村 | スキャフォールドフリー自己組織性三次元人工組織（Ｓｃａｆｆｏｌｄ−ｆｒｅｅＳｅｌｆ−Ｏｒｇａｎｉｚｅｄ３Ｄｓｙｎｔｈｅｔｉｃｔｉｓｓｕｅ） |
| JP2006055106A (ja) | 2004-08-23 | 2006-03-02 | National Institute Of Advanced Industrial & Technology | ヒト血清培地を用いるヒト骨髄由来間葉系幹細胞培養法 |
| KR101107835B1 (ko) * | 2006-01-13 | 2012-02-09 | 가부시키가이샤 투셀 | 동물 세포의 무혈청 배양에 사용하기 위한 배양 배지 첨가물, 키트 및 이의 용도 |
| LV13634B (en) | 2007-05-02 | 2007-12-20 | Jp Biotechnology Sia | Production of ethanol and feedstuffs |
| JPWO2009084662A1 (ja) * | 2007-12-28 | 2011-05-19 | 富士レビオ株式会社 | 哺乳動物体細胞用培地及びそのための添加剤 |
| JP5515319B2 (ja) * | 2009-02-23 | 2014-06-11 | 富士レビオ株式会社 | 脂肪細胞への分化誘導培地及び方法 |
-
2009
- 2009-02-23 JP JP2009038998A patent/JP5663841B2/ja not_active Expired - Fee Related
-
2010
- 2010-02-18 CA CA2753303A patent/CA2753303A1/en not_active Abandoned
- 2010-02-18 WO PCT/JP2010/052455 patent/WO2010095685A1/ja not_active Ceased
- 2010-02-18 EP EP10743811.1A patent/EP2399987B1/en not_active Not-in-force
- 2010-02-18 US US13/202,713 patent/US9315777B2/en not_active Expired - Fee Related
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