JP5657676B2 - 顕微鏡 - Google Patents

顕微鏡 Download PDF

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Publication number
JP5657676B2
JP5657676B2 JP2012530221A JP2012530221A JP5657676B2 JP 5657676 B2 JP5657676 B2 JP 5657676B2 JP 2012530221 A JP2012530221 A JP 2012530221A JP 2012530221 A JP2012530221 A JP 2012530221A JP 5657676 B2 JP5657676 B2 JP 5657676B2
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JP
Japan
Prior art keywords
illumination
light
detection
switching
beam path
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2012530221A
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English (en)
Japanese (ja)
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JP2013506152A (ja
JP2013506152A5 (enExample
Inventor
リッペルト、ヘルムート
ヴォレシェンスキー、ラルフ
ハウシルト、ロベルト
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Carl Zeiss Microscopy GmbH
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Carl Zeiss Microscopy GmbH
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Publication date
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Publication of JP2013506152A publication Critical patent/JP2013506152A/ja
Publication of JP2013506152A5 publication Critical patent/JP2013506152A5/ja
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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
JP2012530221A 2009-09-24 2010-09-17 顕微鏡 Active JP5657676B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102009044986.8 2009-09-24
DE102009044986A DE102009044986A1 (de) 2009-09-24 2009-09-24 Mikroskop
PCT/EP2010/063667 WO2011036096A1 (de) 2009-09-24 2010-09-17 Mikroskop

Publications (3)

Publication Number Publication Date
JP2013506152A JP2013506152A (ja) 2013-02-21
JP2013506152A5 JP2013506152A5 (enExample) 2013-10-10
JP5657676B2 true JP5657676B2 (ja) 2015-01-21

Family

ID=42983344

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2012530221A Active JP5657676B2 (ja) 2009-09-24 2010-09-17 顕微鏡

Country Status (5)

Country Link
US (1) US8792162B2 (enExample)
EP (1) EP2480924B1 (enExample)
JP (1) JP5657676B2 (enExample)
DE (1) DE102009044986A1 (enExample)
WO (1) WO2011036096A1 (enExample)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5692969B2 (ja) * 2008-09-01 2015-04-01 浜松ホトニクス株式会社 収差補正方法、この収差補正方法を用いたレーザ加工方法、この収差補正方法を用いたレーザ照射方法、収差補正装置、及び、収差補正プログラム
DE102011054914A1 (de) 2011-10-28 2013-05-02 Leica Microsystems Cms Gmbh Verfahren und Anordnung zur Beleuchtung einer Probe
WO2015108846A1 (en) 2014-01-14 2015-07-23 Applied Scientific Instrumentation, Inc. Light sheet generator
WO2015164843A1 (en) * 2014-04-24 2015-10-29 Vutara, Inc. Galvo scanning mirror for super-resolution microscopy
DE102014112648A1 (de) 2014-08-29 2016-03-03 Carl Zeiss Ag Bildaufnahmevorrichtung und Verfahren zur Bildaufnahme
DE102014112666A1 (de) * 2014-08-29 2016-03-03 Carl Zeiss Ag Bildaufnahmevorrichtung und Verfahren zur Bildaufnahme
US10181190B2 (en) * 2014-11-04 2019-01-15 Olympus Corporation Microscope and microscope image acquisition method
JP6419532B2 (ja) * 2014-11-04 2018-11-07 オリンパス株式会社 顕微鏡および顕微鏡画像取得方法
JP6391427B2 (ja) * 2014-11-04 2018-09-19 オリンパス株式会社 顕微鏡および顕微鏡画像取得方法
JP6635052B2 (ja) * 2015-02-05 2020-01-22 株式会社ニコン 構造化照明顕微鏡、及び観察方法
LU92695B1 (de) * 2015-04-17 2016-10-18 Leica Microsystems Verfahren und vorrichtung zur spim-untersuchung einer probe
US10989661B2 (en) * 2015-05-01 2021-04-27 The Board Of Regents Of The University Of Texas System Uniform and scalable light-sheets generated by extended focusing
JP6436862B2 (ja) * 2015-06-09 2018-12-12 オリンパス株式会社 顕微鏡および顕微鏡画像取得方法
JP6552881B2 (ja) * 2015-06-12 2019-07-31 オリンパス株式会社 顕微鏡および顕微鏡画像取得方法
US10509215B2 (en) * 2016-03-14 2019-12-17 Olympus Corporation Light-field microscope
US10876970B2 (en) 2016-04-12 2020-12-29 The Board Of Regents Of The University Of Texas System Light-sheet microscope with parallelized 3D image acquisition
DE102016108384B3 (de) 2016-05-04 2017-11-09 Leica Microsystems Cms Gmbh Vorrichtung und Verfahren zur lichtblattartigen Beleuchtung einer Probe
US11579428B2 (en) 2017-07-20 2023-02-14 Viventis Microscopy Sarl Microscope, method of operating a microscope and method of imaging a sample
DE102018210603B4 (de) * 2018-06-28 2025-10-09 Carl Zeiss Microscopy Gmbh Verfahren zum Erzeugen eines Übersichtsbilds unter Verwendung eines hochaperturigen Objektivs
JP7101561B2 (ja) * 2018-08-09 2022-07-15 浜松ホトニクス株式会社 二光子顕微鏡
US10871439B2 (en) * 2018-10-29 2020-12-22 University Of Wyoming Enhancement of sensitivity of fountain flow cytometry by background attenuation
JP7572033B2 (ja) * 2020-10-23 2024-10-23 株式会社リガク 結像型ｘ線顕微鏡
EP4036628A1 (en) 2021-01-22 2022-08-03 Viventis Microscopy Sàrl Microscope for imaging a sample
JP7105006B1 (ja) 2021-03-25 2022-07-22 株式会社CUBICStars 走査型蛍光顕微鏡

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10257423A1 (de) 2002-12-09 2004-06-24 Europäisches Laboratorium für Molekularbiologie (EMBL) Mikroskop
DE102007015063B4 (de) 2007-03-29 2019-10-17 Carl Zeiss Microscopy Gmbh Optische Anordnung zum Erzeugen eines Lichtblattes
DE102007045897A1 (de) 2007-09-26 2009-04-09 Carl Zeiss Microimaging Gmbh Verfahren zur mikroskopischen dreidimensionalen Abbildung einer Probe
DE102007047464B4 (de) * 2007-09-28 2023-03-02 Carl Zeiss Microscopy Gmbh Optische Anordnung zur Photomanipulation
DE102007047461A1 (de) * 2007-09-28 2009-04-02 Carl Zeiss Microimaging Gmbh Verfahren und optische Anordnung zur Untersuchung einer Probe
DE102007063274B8 (de) * 2007-12-20 2022-12-15 Albert-Ludwigs-Universität Freiburg Mikroskop
US9134521B2 (en) * 2008-07-30 2015-09-15 The Regents Of The University Of California Multidirectional selective plane illumination microscopy
DE102011051042B4 (de) * 2011-06-14 2016-04-28 Leica Microsystems Cms Gmbh Abtastmikroskop und Verfahren zur lichtmikroskopischen Abbildung eines Objektes

Also Published As

Publication number Publication date
JP2013506152A (ja) 2013-02-21
US8792162B2 (en) 2014-07-29
US20120176674A1 (en) 2012-07-12
DE102009044986A1 (de) 2011-03-31
EP2480924A1 (de) 2012-08-01
EP2480924B1 (de) 2018-03-21
WO2011036096A1 (de) 2011-03-31

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