JP5515319B2 - 脂肪細胞への分化誘導培地及び方法 - Google Patents

脂肪細胞への分化誘導培地及び方法 Download PDF

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Publication number
JP5515319B2
JP5515319B2 JP2009038977A JP2009038977A JP5515319B2 JP 5515319 B2 JP5515319 B2 JP 5515319B2 JP 2009038977 A JP2009038977 A JP 2009038977A JP 2009038977 A JP2009038977 A JP 2009038977A JP 5515319 B2 JP5515319 B2 JP 5515319B2
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Prior art keywords
medium
differentiation
adipocytes
stem cells
vitamin
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Expired - Fee Related
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JP2009038977A
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English (en)
Japanese (ja)
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JP2010193721A5 (enExample
JP2010193721A (ja
Inventor
佳之 堀田
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Fujirebio Inc
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Fujirebio Inc
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Priority to JP2009038977A priority Critical patent/JP5515319B2/ja
Priority to CA2753299A priority patent/CA2753299C/en
Priority to PCT/JP2010/052454 priority patent/WO2010095684A1/ja
Priority to US13/202,720 priority patent/US8785199B2/en
Priority to EP10743810.3A priority patent/EP2399989B1/en
Publication of JP2010193721A publication Critical patent/JP2010193721A/ja
Publication of JP2010193721A5 publication Critical patent/JP2010193721A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0653—Adipocytes; Adipose tissue

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Rheumatology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2009038977A 2009-02-23 2009-02-23 脂肪細胞への分化誘導培地及び方法 Expired - Fee Related JP5515319B2 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2009038977A JP5515319B2 (ja) 2009-02-23 2009-02-23 脂肪細胞への分化誘導培地及び方法
CA2753299A CA2753299C (en) 2009-02-23 2010-02-18 Culture medium and method for inducing differentiation into adipocytes
PCT/JP2010/052454 WO2010095684A1 (ja) 2009-02-23 2010-02-18 脂肪細胞への分化誘導培地及び方法
US13/202,720 US8785199B2 (en) 2009-02-23 2010-02-18 Culture medium and method for inducing differentiation into adipocytes
EP10743810.3A EP2399989B1 (en) 2009-02-23 2010-02-18 Culture medium and method for inducing differentiation into adipocytes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2009038977A JP5515319B2 (ja) 2009-02-23 2009-02-23 脂肪細胞への分化誘導培地及び方法

Publications (3)

Publication Number Publication Date
JP2010193721A JP2010193721A (ja) 2010-09-09
JP2010193721A5 JP2010193721A5 (enExample) 2012-04-05
JP5515319B2 true JP5515319B2 (ja) 2014-06-11

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JP2009038977A Expired - Fee Related JP5515319B2 (ja) 2009-02-23 2009-02-23 脂肪細胞への分化誘導培地及び方法

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Country Link
US (1) US8785199B2 (enExample)
EP (1) EP2399989B1 (enExample)
JP (1) JP5515319B2 (enExample)
CA (1) CA2753299C (enExample)
WO (1) WO2010095684A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101877793B1 (ko) * 2016-07-15 2018-07-13 주식회사 엔바이오텍 줄기세포 배양용 무혈청 배지 조성물 및 이를 이용한 줄기세포의 배양 방법

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5663841B2 (ja) * 2009-02-23 2015-02-04 富士レビオ株式会社 骨細胞への分化誘導培地及び方法
WO2013173688A1 (en) * 2012-05-18 2013-11-21 The Cooper Health System A method of making tissue engineered blood vessels and generating capillary networks, articles, and methods of use thereof
WO2018002930A1 (en) 2016-06-30 2018-01-04 Stem Cell Medicine Ltd. Treatment of inflammatory bowel disease with long acting glatiramer and adipose-derived stem cells
CN108130310A (zh) * 2016-11-30 2018-06-08 江阴瑞康健生物医学科技有限公司 一种干细胞培养基
CN110709091A (zh) 2017-05-15 2020-01-17 干细胞医药有限公司 使用长效格拉替雷和脂肪源性干细胞治疗多发性硬化症
AU2018270396B2 (en) 2017-05-15 2025-03-20 Stem Cell Medicine Ltd. Treatment of multiple sclerosis with adipose-derived stem cells
CN110257324A (zh) * 2019-07-24 2019-09-20 安徽科门生物科技有限公司 一种用于脂肪间充质干细胞成脂诱导的培养基以及诱导方法
CN113337457B (zh) * 2021-06-01 2024-06-25 澳门大学 一种无血清调控干细胞的细胞状态的方法以及调节剂的应用
CN115927172A (zh) * 2022-12-15 2023-04-07 南京周子未来食品科技有限公司 一种诱导体外成脂分化的化学成分明确的改良培养基及其应用
CN116162587B (zh) * 2023-02-20 2025-07-25 杭州瑞普晨创科技有限公司 Hippo-Yap通路调控剂在促进诱导性多能干细胞向胰腺前体细胞定向分化中的应用
CN119842599B (zh) * 2025-03-21 2025-07-22 中国海洋大学 一种鱼类干细胞无血清诱导成脂分化培养基及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003230792A1 (en) * 2002-04-03 2003-10-20 Artecel Sciences, Inc. Improvements of adipocytic differentiated adipose derived adult stem cells and uses thereof
JP2008533114A (ja) 2005-03-18 2008-08-21 ユーシーエル ビジネス パブリック リミテッド カンパニー メカノ成長因子ペプチドおよびその使用
US20110008893A1 (en) 2007-12-28 2011-01-13 Fujirebio Inc. Medium for mammalian somatic cells and additive therefor

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101877793B1 (ko) * 2016-07-15 2018-07-13 주식회사 엔바이오텍 줄기세포 배양용 무혈청 배지 조성물 및 이를 이용한 줄기세포의 배양 방법

Also Published As

Publication number Publication date
US8785199B2 (en) 2014-07-22
EP2399989A4 (en) 2013-01-16
JP2010193721A (ja) 2010-09-09
EP2399989B1 (en) 2015-04-08
CA2753299C (en) 2016-09-13
US20110306133A1 (en) 2011-12-15
CA2753299A1 (en) 2010-08-26
WO2010095684A1 (ja) 2010-08-26
EP2399989A1 (en) 2011-12-28

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