JP5512177B2 - 胞子形成能低下納豆菌株および該株を用いて製造した胞子数の少ない納豆 - Google Patents
胞子形成能低下納豆菌株および該株を用いて製造した胞子数の少ない納豆 Download PDFInfo
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Description
育種親株としては、宮城野納豆菌より分離されたNAFM5を用いた。また、納豆菌の形質転換はAnagnostopoulos C.、Spizizen J., 1961 " Requirements for transformation in Bacillus subtilis." J. Bacteriol. 81:741-746に記載の方法により行った。
親株、および、spo0A遺伝子、sigH遺伝子、sigE遺伝子、sigF遺伝子、sigK遺伝子およびsigG遺伝子の各破壊株を用いて実際に納豆を試作し、これら破壊株の納豆での効果を確認した。納豆の種菌としては、−80℃で凍結保存しておいたこれらの破壊株を、いずれもLB液体培地で培養し、得られた培養液を滅菌水で1,000倍に希釈したものを種菌液とした。大豆は一晩常温にて水につけておいた丸大豆を、ざるに入れ、121℃で50分間オートクレーブして煮豆とした。この煮豆がさめてから種菌液を50gあたり1mlの割合で接種し、40℃で17時間発酵させた。その後4℃で3日間保存して熟成を行い、納豆とした。また、コントロールとして親株であるNAFM5についても同様の手順で納豆を試作した。
本発明の納豆菌変異株によれば、納豆の品質に影響を与えることなく、納豆菌胞子の少ない納豆を製造することが可能である。従って、除菌・殺菌が困難な納豆菌胞子の混入の危険性が低下することから、除菌・殺菌が通常の納豆よりも容易であり、納豆を原材料とする加工食品への利用拡大が一段と期待出来る。
Claims (5)
- 胞子形成能が低下した納豆菌変異株であって、
sigE遺伝子、sigF遺伝子、sigK遺伝子およびsigG遺伝子からなる群から選択される少なくとも一つの胞子形成遺伝子が欠損されている、納豆菌変異株。 - 胞子形成遺伝子の欠損が、該遺伝子の薬剤耐性遺伝子での置換によるものである、請求項1に記載の納豆菌変異株。
- sigK遺伝子がカナマイシン耐性遺伝子で置換されている、胞子形成能低下納豆菌変異株 Bacillus subtilis(natto)SPDK(FERM P−21811)。
- 請求項1〜3のいずれか一項に記載の納豆菌変異株を用いて製造されたことを特徴とする納豆。
- 納豆中の胞子数が、納豆1g当たり103個以下であり、かつ、グルタミン酸含量が親株よりも多い、請求項4に記載の納豆。
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CN108949785A (zh) * | 2018-08-06 | 2018-12-07 | 齐鲁工业大学 | 芽孢形成相关基因spo0A在产酶中的应用 |
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JP6019528B2 (ja) * | 2012-06-05 | 2016-11-02 | 池田食研株式会社 | 発酵調味料の製造方法 |
JP2018139542A (ja) * | 2017-02-28 | 2018-09-13 | 株式会社Mizkan Holdings | 新規温度感受性納豆菌、及び胞子低含有納豆 |
JP6963288B2 (ja) | 2017-02-28 | 2021-11-05 | 株式会社Mizkan Holdings | 新規温度感受性納豆菌、及び胞子低含有納豆 |
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JPH03297358A (ja) * | 1990-04-13 | 1991-12-27 | Asahimatsu Shokuhin Kk | ビタミンk含量の低い納豆の製造方法 |
JP4104741B2 (ja) * | 1998-09-08 | 2008-06-18 | 旭松食品株式会社 | ビタミンk高生産性菌株及びそれを用いたビタミンkの生産方法 |
JP4574103B2 (ja) * | 2002-04-02 | 2010-11-04 | 旭松食品株式会社 | グルタミン酸高生産性納豆菌株およびそれを用いて作られるグルタミン酸高含有納豆 |
JP4713469B2 (ja) * | 2003-06-18 | 2011-06-29 | ディーエスエム アイピー アセッツ ビー.ブイ. | 胞子形成できない微生物を用いたパントテネート(Pantothenate)の製造 |
JP4113860B2 (ja) * | 2004-06-22 | 2008-07-09 | 株式会社ヤマダフーズ | 無菌納豆の製造方法 |
JP4376740B2 (ja) * | 2004-09-08 | 2009-12-02 | 旭松食品株式会社 | 多糖類低生産性納豆菌 |
JP5140307B2 (ja) * | 2007-04-10 | 2013-02-06 | 花王株式会社 | 組換え微生物 |
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CN108949785A (zh) * | 2018-08-06 | 2018-12-07 | 齐鲁工业大学 | 芽孢形成相关基因spo0A在产酶中的应用 |
CN108949785B (zh) * | 2018-08-06 | 2020-03-06 | 齐鲁工业大学 | 芽孢形成相关基因spo0A在产酶中的应用 |
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