JP5409631B2 - 酵素反応においてサンプル中でｃＤＮＡを合成するための方法 - Google Patents

酵素反応においてサンプル中でｃＤＮＡを合成するための方法 Download PDF

Info

Publication number
JP5409631B2
JP5409631B2 JP2010520506A JP2010520506A JP5409631B2 JP 5409631 B2 JP5409631 B2 JP 5409631B2 JP 2010520506 A JP2010520506 A JP 2010520506A JP 2010520506 A JP2010520506 A JP 2010520506A JP 5409631 B2 JP5409631 B2 JP 5409631B2
Authority
JP
Japan
Prior art keywords
reaction
poly
enzyme
activity
reverse transcriptase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2010520506A
Other languages
English (en)
Japanese (ja)
Other versions
JP2010535525A5 (enExample
JP2010535525A (ja
Inventor
ホルガー エンゲル
スブラーマニャム イェーラミーリ
マルティン クロイツ
ディルク レッフェルト
クリスチャン コルフハーゲ
Original Assignee
キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/EP2007/058369 external-priority patent/WO2008020008A1/de
Application filed by キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング filed Critical キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング
Publication of JP2010535525A publication Critical patent/JP2010535525A/ja
Publication of JP2010535525A5 publication Critical patent/JP2010535525A5/ja
Application granted granted Critical
Publication of JP5409631B2 publication Critical patent/JP5409631B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2010520506A 2007-08-13 2008-02-13 酵素反応においてサンプル中でｃＤＮＡを合成するための方法 Active JP5409631B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EPPCT/EP2007/058369 2007-08-13
PCT/EP2007/058369 WO2008020008A1 (de) 2006-08-14 2007-08-13 VERFAHREN ZUR SYNTHESE EINER cDNA IN EINER PROBE IN EINER ENZYMATISCHEN REAKTION
PCT/EP2008/051763 WO2009021757A1 (de) 2007-08-13 2008-02-13 Verfahren zur synthese einer cdna in einer probe in einer enzymatischen reaktion

Publications (3)

Publication Number Publication Date
JP2010535525A JP2010535525A (ja) 2010-11-25
JP2010535525A5 JP2010535525A5 (enExample) 2011-01-20
JP5409631B2 true JP5409631B2 (ja) 2014-02-05

Family

ID=39322429

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2010520506A Active JP5409631B2 (ja) 2007-08-13 2008-02-13 酵素反応においてサンプル中でｃＤＮＡを合成するための方法

Country Status (3)

Country Link
JP (1) JP5409631B2 (enExample)
AU (1) AU2008286254B2 (enExample)
WO (1) WO2009021757A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5553323B2 (ja) * 2012-01-25 2014-07-16 独立行政法人科学技術振興機構 Ｈｉｖ検出キット、及びｈｉｖ検出方法
WO2016092062A1 (en) * 2014-12-11 2016-06-16 Qiagen Gmbh Method for improving the efficiency of cdna generation
US12247200B2 (en) * 2020-02-21 2025-03-11 Bioo Scientific Corporation Compositions and methods for generating massively parallel nucleic acid sequencing libraries
CN113151398A (zh) * 2021-05-07 2021-07-23 广州复能基因有限公司 外泌体中核酸分子的检测方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2322073C (en) * 1998-02-27 2003-12-23 Pamgene B.V. Method for the non-specific amplification of nucleic acid
US20070128598A1 (en) * 2002-10-30 2007-06-07 Piet Boender Methods for generating rna copies
US20050196782A1 (en) * 2003-12-23 2005-09-08 Kiefer Michael C. Universal amplification of fragmented RNA
CN1763223B (zh) * 2004-10-19 2011-04-27 中国人民解放军军事医学科学院放射与辐射医学研究所 miRNA检测方法
DE102006038113A1 (de) * 2006-08-14 2008-02-21 Qiagen Gmbh Verfahren zur Synthese einer cDNA in einer Probe in einer enzymatischen Reaktion und gleichzeitigen Bereitstellung eines ersten Enzyms mit Polyadenylierungsaktivität und eines zweiten Enzyms mit reverser Transkriptaseaktivität
US8314220B2 (en) * 2007-01-26 2012-11-20 Agilent Technologies, Inc. Methods compositions, and kits for detection of microRNA

Also Published As

Publication number Publication date
AU2008286254A1 (en) 2009-02-19
WO2009021757A1 (de) 2009-02-19
AU2008286254B2 (en) 2014-01-30
JP2010535525A (ja) 2010-11-25

Similar Documents

Publication Publication Date Title
JP5409360B2 (ja) 酵素反応における試料中のｃＤＮＡの合成方法
JP6768706B2 (ja) 液相におけるライゲーションアッセイ
EP3058104B1 (en) Methods for adding adapters to nucleic acids and compositions for practicing the same
CA2877368C (en) Kit for isothermal dna amplification starting from an rna template
CN114250278B (zh) 捕获、检测和定量小rna的方法、组合物与试剂盒
JP5680080B2 (ja) アンカーオリゴヌクレオチドおよびアダプターオリゴヌクレオチドを用いる核酸の正規化した定量化方法
JP5680078B2 (ja) ライゲーションに基づく核酸の正規化した定量化方法
JP2014057599A (ja) ライゲーション増幅
WO2008097957A2 (en) Detection of mature small rna molecules
CN102112631A (zh) 用于mRNA扩增的改进的裂解和逆转录
JP5409631B2 (ja) 酵素反応においてサンプル中でｃＤＮＡを合成するための方法
JP5680079B2 (ja) Ｒｎａの正規化した定量化方法
US9777319B2 (en) Method for isothermal DNA amplification starting from an RNA template
US20140004509A1 (en) Kit for isothermal dna amplification starting from an rna template
US20050009047A1 (en) Global linear non-biased nucleic acid amplification
JP2003070490A (ja) 核酸配列の増幅方法
CN118302539A (zh) 扩增样品中的rna的方法
US9587267B2 (en) Quantification of nucleic acids
EP2191008A1 (de) Verfahren zur synthese einer cdna in einer probe in einer enzymatischen reaktion

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20101110

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20101110

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20130305

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130604

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20130806

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130930

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20131022

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20131105

R150 Certificate of patent or registration of utility model

Ref document number: 5409631

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250