AU2008286254B2 - Method for synthesizing a cDNA in a sample in an enzymatic reaction - Google Patents

Method for synthesizing a cDNA in a sample in an enzymatic reaction Download PDF

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Publication number
AU2008286254B2
AU2008286254B2 AU2008286254A AU2008286254A AU2008286254B2 AU 2008286254 B2 AU2008286254 B2 AU 2008286254B2 AU 2008286254 A AU2008286254 A AU 2008286254A AU 2008286254 A AU2008286254 A AU 2008286254A AU 2008286254 B2 AU2008286254 B2 AU 2008286254B2
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AU
Australia
Prior art keywords
reaction
poly
enzyme
primer
pcr
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AU2008286254A
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English (en)
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AU2008286254A1 (en
Inventor
Holger Engel
Christian Korfhage
Martin Kreutz
Dirk Loffert
Subrahmanyam Yerramilli
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Qiagen GmbH
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Qiagen GmbH
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Priority claimed from PCT/EP2007/058369 external-priority patent/WO2008020008A1/de
Application filed by Qiagen GmbH filed Critical Qiagen GmbH
Publication of AU2008286254A1 publication Critical patent/AU2008286254A1/en
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Publication of AU2008286254B2 publication Critical patent/AU2008286254B2/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2008286254A 2007-08-13 2008-02-13 Method for synthesizing a cDNA in a sample in an enzymatic reaction Active AU2008286254B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AUPCT/EP2007/058369 2007-08-13
PCT/EP2007/058369 WO2008020008A1 (de) 2006-08-14 2007-08-13 VERFAHREN ZUR SYNTHESE EINER cDNA IN EINER PROBE IN EINER ENZYMATISCHEN REAKTION
PCT/EP2008/051763 WO2009021757A1 (de) 2007-08-13 2008-02-13 Verfahren zur synthese einer cdna in einer probe in einer enzymatischen reaktion

Publications (2)

Publication Number Publication Date
AU2008286254A1 AU2008286254A1 (en) 2009-02-19
AU2008286254B2 true AU2008286254B2 (en) 2014-01-30

Family

ID=39322429

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2008286254A Active AU2008286254B2 (en) 2007-08-13 2008-02-13 Method for synthesizing a cDNA in a sample in an enzymatic reaction

Country Status (3)

Country Link
JP (1) JP5409631B2 (enExample)
AU (1) AU2008286254B2 (enExample)
WO (1) WO2009021757A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5553323B2 (ja) * 2012-01-25 2014-07-16 独立行政法人科学技術振興機構 Ｈｉｖ検出キット、及びｈｉｖ検出方法
WO2016092062A1 (en) * 2014-12-11 2016-06-16 Qiagen Gmbh Method for improving the efficiency of cdna generation
US12247200B2 (en) * 2020-02-21 2025-03-11 Bioo Scientific Corporation Compositions and methods for generating massively parallel nucleic acid sequencing libraries
CN113151398A (zh) * 2021-05-07 2021-07-23 广州复能基因有限公司 外泌体中核酸分子的检测方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030113875A1 (en) * 1998-02-27 2003-06-19 Gemen Bob Van Method for the non-specific amplification of nucleic acid
WO2004044239A1 (en) * 2002-10-30 2004-05-27 Pamgene B.V. Improved methods for generating multiple rna copies
CN1763223A (zh) * 2004-10-19 2006-04-26 中国人民解放军军事医学科学院放射医学研究所 miRNA检测方法及试剂盒
WO2008092016A2 (en) * 2007-01-26 2008-07-31 Stratagene California Methods, compositions, and kits for detection of micro rna

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050196782A1 (en) * 2003-12-23 2005-09-08 Kiefer Michael C. Universal amplification of fragmented RNA
DE102006038113A1 (de) * 2006-08-14 2008-02-21 Qiagen Gmbh Verfahren zur Synthese einer cDNA in einer Probe in einer enzymatischen Reaktion und gleichzeitigen Bereitstellung eines ersten Enzyms mit Polyadenylierungsaktivität und eines zweiten Enzyms mit reverser Transkriptaseaktivität

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030113875A1 (en) * 1998-02-27 2003-06-19 Gemen Bob Van Method for the non-specific amplification of nucleic acid
WO2004044239A1 (en) * 2002-10-30 2004-05-27 Pamgene B.V. Improved methods for generating multiple rna copies
CN1763223A (zh) * 2004-10-19 2006-04-26 中国人民解放军军事医学科学院放射医学研究所 miRNA检测方法及试剂盒
WO2008092016A2 (en) * 2007-01-26 2008-07-31 Stratagene California Methods, compositions, and kits for detection of micro rna

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KO, J-H., and LEE, Y., Journal of Microbiological Methods, 2006, vol. 64, pp297-304 *

Also Published As

Publication number Publication date
AU2008286254A1 (en) 2009-02-19
WO2009021757A1 (de) 2009-02-19
JP5409631B2 (ja) 2014-02-05
JP2010535525A (ja) 2010-11-25

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