JP5368108B2 - 可逆的停止ヌクレオチドを利用する配列決定法および遺伝型解析法 - Google Patents

可逆的停止ヌクレオチドを利用する配列決定法および遺伝型解析法 Download PDF

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JP5368108B2
JP5368108B2 JP2008547582A JP2008547582A JP5368108B2 JP 5368108 B2 JP5368108 B2 JP 5368108B2 JP 2008547582 A JP2008547582 A JP 2008547582A JP 2008547582 A JP2008547582 A JP 2008547582A JP 5368108 B2 JP5368108 B2 JP 5368108B2
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reversible terminator
nucleic acid
modified
nucleotide
terminator nucleotide
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JP2009521223A (ja
JP2009521223A5 (enExample
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エイチ． ジェルファンド，デイビッド
ピー． グプタ，エイマー
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Life Sciences & Earth Sciences (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
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JP2008547582A 2005-12-21 2006-12-20 可逆的停止ヌクレオチドを利用する配列決定法および遺伝型解析法 Active JP5368108B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US75282705P 2005-12-21 2005-12-21
US60/752,827 2005-12-21
US84404106P 2006-09-11 2006-09-11
US60/844,041 2006-09-11
PCT/US2006/048933 WO2007075967A2 (en) 2005-12-21 2006-12-20 Sequencing and genotyping using reversibly 2'-modified nucleotides

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JP2009521223A JP2009521223A (ja) 2009-06-04
JP2009521223A5 JP2009521223A5 (enExample) 2010-02-25
JP5368108B2 true JP5368108B2 (ja) 2013-12-18

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US (1) US9206476B2 (enExample)
EP (1) EP1974057B1 (enExample)
JP (1) JP5368108B2 (enExample)
CN (1) CN101346472B (enExample)
CA (1) CA2631316C (enExample)
ES (1) ES2388722T3 (enExample)
WO (1) WO2007075967A2 (enExample)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7947817B2 (en) 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
US7572581B2 (en) 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
US7928207B2 (en) * 2004-06-28 2011-04-19 Roche Molecular Systems, Inc Synthesis and compositions of nucleic acids comprising 2′-terminator nucleotides
US7745125B2 (en) * 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
US8212020B2 (en) * 2005-03-11 2012-07-03 Steven Albert Benner Reagents for reversibly terminating primer extension
WO2007075967A2 (en) 2005-12-21 2007-07-05 Roche Diagnostics Gmbh Sequencing and genotyping using reversibly 2'-modified nucleotides
US8399188B2 (en) 2006-09-28 2013-03-19 Illumina, Inc. Compositions and methods for nucleotide sequencing
US8703422B2 (en) * 2007-06-06 2014-04-22 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
AU2008261935B2 (en) * 2007-06-06 2013-05-02 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
WO2013154750A1 (en) 2012-04-10 2013-10-17 The Trustees Of Columbia Unversity In The City Of New York Systems and methods for biological ion channel interfaces
US20100255487A1 (en) 2009-03-27 2010-10-07 Life Technologies Corporation Methods and apparatus for single molecule sequencing using energy transfer detection
WO2010127304A2 (en) * 2009-05-01 2010-11-04 Illumina, Inc. Sequencing methods
WO2010141391A2 (en) 2009-06-05 2010-12-09 Life Technologies Corporation Mutant dna polymerases
US20100330556A1 (en) * 2009-06-30 2010-12-30 Brian Jon Peter Genome analysis using a nicking endonuclease
DK2456892T3 (en) 2009-07-24 2014-12-08 Illumina Inc Procedure for sequencing of a polynukleotidskabelon
US8965076B2 (en) 2010-01-13 2015-02-24 Illumina, Inc. Data processing system and methods
EP3444600B1 (en) * 2011-01-11 2020-05-13 The Trustees of Columbia University in the City of New York System and methods for single-molecule detection using nanotubes
CN103380369B (zh) 2011-02-23 2016-12-28 纽约市哥伦比亚大学理事会 使用纳米孔进行单分子检测的系统和方法
US10378051B2 (en) * 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
JP6456816B2 (ja) * 2012-04-09 2019-01-23 ザ・トラスティーズ・オブ・コランビア・ユニバーシティー・イン・ザ・シティー・オブ・ニューヨーク ナノ細孔の調製方法およびその使用
WO2013158280A1 (en) 2012-04-20 2013-10-24 The Trustees Of Columbia University In The City Of New York Systems and methods for single-molecule nucleic-acid assay platforms
US9770713B2 (en) * 2012-08-30 2017-09-26 Hitachi High-Technologies Corporation Nucleic acid analysis device
US9650406B2 (en) 2013-02-28 2017-05-16 Centrillion Technology Holdings Corporation Reversible terminator molecules and methods of their use
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
EP2970366B1 (en) 2013-03-15 2019-01-16 Ibis Biosciences, Inc. Nucleotide analogs for sequencing
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
US11180522B2 (en) 2015-05-08 2021-11-23 Centrillion Technology Holdings Corporation Disulfide-linked reversible terminators
JP2019512209A (ja) 2016-04-22 2019-05-16 オムニオム インコーポレイテッドＯｍｎｉｏｍｅ， Ｉｎｃ． ヌクレオチド特異的三元複合体形成の増強された検出を利用した核酸配列決定方法及びシステム
AU2017268466C1 (en) * 2016-05-20 2022-04-07 Quantum-Si Incorporated Labeled nucleotide compositions and methods for nucleic acid sequencing
JP7058228B2 (ja) * 2016-06-24 2022-04-21 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 繋ぎ止められたヌクレオシド三リン酸を用いた核酸合成および配列決定
CN108239669B (zh) * 2016-12-23 2021-03-16 深圳华大智造科技股份有限公司 检测可逆性阻断dNTP中非阻断杂质含量的方法和试剂盒
WO2018136487A1 (en) 2017-01-20 2018-07-26 Omniome, Inc. Process for cognate nucleotide detection in a nucleic acid sequencing workflow
WO2018213787A1 (en) * 2017-05-18 2018-11-22 Ultima Genomics, Inc. Sequencing using non-natural nucleotides
TWI829642B (zh) 2017-07-24 2024-01-21 美商寬騰矽公司 高強度經標記之反應物組合物及用於定序之方法
WO2019099420A1 (en) * 2017-11-15 2019-05-23 Yan Wang A method for detecting multiple dna mutations and copy number variations
US20190241945A1 (en) * 2018-02-06 2019-08-08 Omniome, Inc. Compositions and techniques for nucleic acid primer extension
WO2020014681A1 (en) 2018-07-13 2020-01-16 Quantum-Si Incorporated Biconjugatable labels and methods of use
EP3856753A4 (en) 2018-09-28 2022-11-16 Centrillion Technology Holdings Corporation DISULFIDE CROSSLINKED REVERSIBLE TERMINATORS
CN113614097A (zh) 2019-01-23 2021-11-05 宽腾矽公司 高强度标记的反应物组合物和测序方法
US20220356500A1 (en) * 2019-12-30 2022-11-10 Yuandian Biolabs Co., Ltd. Method for preparing nucleic acid sequences using enzyme
KR20220104057A (ko) * 2019-12-30 2022-07-25 위안디안 바이오랩스 컴퍼니 리미티드 효소를 이용한 핵산 서열 제조 장치 및 방법
WO2021185320A1 (en) * 2020-03-18 2021-09-23 Mgi Tech Co., Ltd. Restoring phase in massively parallel sequencing

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
US5436143A (en) * 1992-12-23 1995-07-25 Hyman; Edward D. Method for enzymatic synthesis of oligonucleotides
US5532130A (en) * 1993-07-20 1996-07-02 Dyad Pharmaceutical Corporation Methods and compositions for sequence-specific hybridization of RNA by 2'-5' oligonucleotides
EP1159453B1 (en) * 1999-03-10 2008-05-28 ASM Scientific, Inc. A method for direct nucleic acid sequencing
US7572581B2 (en) * 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
US7947817B2 (en) * 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
EP1753774B1 (en) * 2004-06-02 2008-09-10 ASM Scientific, Inc. 2"-nitrobenzyl-modified ribonucleotides
US7745125B2 (en) * 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
WO2007075967A2 (en) 2005-12-21 2007-07-05 Roche Diagnostics Gmbh Sequencing and genotyping using reversibly 2'-modified nucleotides
EP1987944B1 (de) 2007-05-04 2012-07-25 Leister Technologies AG Laserdurchstrahlschweissvorrichtung und Verfahren zum Verbinden von Kunststoffwerkstücken

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ES2388722T3 (es) 2012-10-18
WO2007075967A2 (en) 2007-07-05
WO2007075967B1 (en) 2008-02-21
EP1974057B1 (en) 2012-06-13
JP2009521223A (ja) 2009-06-04
EP1974057A2 (en) 2008-10-01
US9206476B2 (en) 2015-12-08
CN101346472B (zh) 2013-04-24
CA2631316C (en) 2012-12-04
WO2007075967A3 (en) 2008-01-03
CN101346472A (zh) 2009-01-14
HK1124640A1 (en) 2009-07-17
US20080293071A1 (en) 2008-11-27
CA2631316A1 (en) 2007-07-05

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