CN101346472B - 用可逆2’-修饰的核苷酸进行的序列测定和基因分型 - Google Patents

用可逆2’-修饰的核苷酸进行的序列测定和基因分型 Download PDF

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CN101346472B
CN101346472B CN2006800488413A CN200680048841A CN101346472B CN 101346472 B CN101346472 B CN 101346472B CN 2006800488413 A CN2006800488413 A CN 2006800488413A CN 200680048841 A CN200680048841 A CN 200680048841A CN 101346472 B CN101346472 B CN 101346472B
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nucleotide
nucleic acid
modified
modified reversible
phosphate group
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CN101346472A (zh
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D·H·格尔芬德
A·P·古普塔
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Life Sciences & Earth Sciences (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
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  • General Health & Medical Sciences (AREA)
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CN2006800488413A 2005-12-21 2006-12-20 用可逆2’-修饰的核苷酸进行的序列测定和基因分型 Active CN101346472B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US75282705P 2005-12-21 2005-12-21
US60/752,827 2005-12-21
US84404106P 2006-09-11 2006-09-11
US60/844,041 2006-09-11
PCT/US2006/048933 WO2007075967A2 (en) 2005-12-21 2006-12-20 Sequencing and genotyping using reversibly 2'-modified nucleotides

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CN101346472A CN101346472A (zh) 2009-01-14
CN101346472B true CN101346472B (zh) 2013-04-24

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US (1) US9206476B2 (enExample)
EP (1) EP1974057B1 (enExample)
JP (1) JP5368108B2 (enExample)
CN (1) CN101346472B (enExample)
CA (1) CA2631316C (enExample)
ES (1) ES2388722T3 (enExample)
WO (1) WO2007075967A2 (enExample)

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US8212020B2 (en) * 2005-03-11 2012-07-03 Steven Albert Benner Reagents for reversibly terminating primer extension
EP1974057B1 (en) 2005-12-21 2012-06-13 Roche Diagnostics GmbH Sequencing and genotyping using reversibly 2'-modified nucleotides
US8399188B2 (en) 2006-09-28 2013-03-19 Illumina, Inc. Compositions and methods for nucleotide sequencing
AU2008261935B2 (en) * 2007-06-06 2013-05-02 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
US8703422B2 (en) * 2007-06-06 2014-04-22 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
WO2013154750A1 (en) 2012-04-10 2013-10-17 The Trustees Of Columbia Unversity In The City Of New York Systems and methods for biological ion channel interfaces
US8603792B2 (en) 2009-03-27 2013-12-10 Life Technologies Corporation Conjugates of biomolecules to nanoparticles
WO2010127304A2 (en) * 2009-05-01 2010-11-04 Illumina, Inc. Sequencing methods
US20100311144A1 (en) 2009-06-05 2010-12-09 Life Technologies Corporation Mutant dna polymerases
US20100330556A1 (en) * 2009-06-30 2010-12-30 Brian Jon Peter Genome analysis using a nicking endonuclease
WO2011011175A2 (en) 2009-07-24 2011-01-27 Illumina, Inc. Method for sequencing a polynucleotide template
US8965076B2 (en) 2010-01-13 2015-02-24 Illumina, Inc. Data processing system and methods
WO2012097074A2 (en) 2011-01-11 2012-07-19 The Trustees Of Columbia University In The City Of New York Systems and methods for single-molecule detection using nanotubes
EP2678669A1 (en) 2011-02-23 2014-01-01 The Trustees of Columbia University in the City of New York Systems and methods for single-molecule detection using nanopores
US10378051B2 (en) * 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
CN107082792A (zh) * 2012-04-09 2017-08-22 纽约哥伦比亚大学理事会 纳米孔的制备方法和其用途
WO2013158280A1 (en) 2012-04-20 2013-10-24 The Trustees Of Columbia University In The City Of New York Systems and methods for single-molecule nucleic-acid assay platforms
EP2891709A4 (en) * 2012-08-30 2016-04-13 Hitachi High Tech Corp NUCLEIC ANALYSIS DEVICE
US9650406B2 (en) 2013-02-28 2017-05-16 Centrillion Technology Holdings Corporation Reversible terminator molecules and methods of their use
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
US10428379B2 (en) 2013-03-15 2019-10-01 Ibis Biosciences, Inc. Nucleotide analogs for sequencing
US11180522B2 (en) 2015-05-08 2021-11-23 Centrillion Technology Holdings Corporation Disulfide-linked reversible terminators
CN109072297B (zh) 2016-04-22 2022-09-13 加利福尼亚太平洋生物科学股份有限公司 使用增强的核苷酸特异性三元复合物形成检测的核酸测序方法和系统
WO2017201514A1 (en) * 2016-05-20 2017-11-23 Quantum-Si Incorporated Labeled nucleotide compositions and methods for nucleic acid sequencing
CN114874337A (zh) * 2016-06-24 2022-08-09 加利福尼亚大学董事会 使用栓系的核苷三磷酸的核酸合成和测序
CN108239669B (zh) * 2016-12-23 2021-03-16 深圳华大智造科技股份有限公司 检测可逆性阻断dNTP中非阻断杂质含量的方法和试剂盒
US10975427B2 (en) 2017-01-20 2021-04-13 Omniome, Inc. Process for cognate nucleotide detection in a nucleic acid sequencing workflow
WO2018213787A1 (en) 2017-05-18 2018-11-22 Ultima Genomics, Inc. Sequencing using non-natural nucleotides
WO2019023257A1 (en) 2017-07-24 2019-01-31 Quantum-Si Incorporated HIGH INTENSITY MARKED REAGENT COMPOSITIONS AND SEQUENCING METHODS
WO2019099420A1 (en) * 2017-11-15 2019-05-23 Yan Wang A method for detecting multiple dna mutations and copy number variations
AU2019217824A1 (en) * 2018-02-06 2020-07-30 Omniome, Inc. Compositions and techniques for nucleic acid primer extension
MX2021000470A (es) 2018-07-13 2021-06-23 Quantum Si Inc Etiquetas biconjugables y métodos de uso.
WO2020069424A1 (en) 2018-09-28 2020-04-02 Centrillion Technologies, Inc. Disulfide-linked reversible terminators
US11613772B2 (en) 2019-01-23 2023-03-28 Quantum-Si Incorporated High intensity labeled reactant compositions and methods for sequencing
EP4085063A4 (en) * 2019-12-30 2023-09-06 Yuandian Biolabs Co., Ltd. METHOD FOR PREPARING NUCLEIC ACID SEQUENCES USING AN ENZYME
KR20220104057A (ko) * 2019-12-30 2022-07-25 위안디안 바이오랩스 컴퍼니 리미티드 효소를 이용한 핵산 서열 제조 장치 및 방법
US20230129228A1 (en) * 2020-03-18 2023-04-27 Mgi Tech Co. Ltd. Restoring phase in massively parallel sequencing

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US20050037398A1 (en) * 2003-06-30 2005-02-17 Roche Molecular Systems, Inc. 2'-terminator nucleotide-related methods and systems
WO2005118608A2 (en) * 2004-06-02 2005-12-15 Asm Scientific, Inc. 2’-nitrobenzyl-modified ribonucleotides

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US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
US5436143A (en) * 1992-12-23 1995-07-25 Hyman; Edward D. Method for enzymatic synthesis of oligonucleotides
US5532130A (en) * 1993-07-20 1996-07-02 Dyad Pharmaceutical Corporation Methods and compositions for sequence-specific hybridization of RNA by 2'-5' oligonucleotides
AU3174600A (en) * 1999-03-10 2000-09-28 Asm Scientific, Inc. A method for direct nucleic acid sequencing
US7947817B2 (en) * 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
US7745125B2 (en) * 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
EP1974057B1 (en) 2005-12-21 2012-06-13 Roche Diagnostics GmbH Sequencing and genotyping using reversibly 2'-modified nucleotides
EP1987944B1 (de) 2007-05-04 2012-07-25 Leister Technologies AG Laserdurchstrahlschweissvorrichtung und Verfahren zum Verbinden von Kunststoffwerkstücken

Patent Citations (2)

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US20050037398A1 (en) * 2003-06-30 2005-02-17 Roche Molecular Systems, Inc. 2'-terminator nucleotide-related methods and systems
WO2005118608A2 (en) * 2004-06-02 2005-12-15 Asm Scientific, Inc. 2’-nitrobenzyl-modified ribonucleotides

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US20080293071A1 (en) 2008-11-27
US9206476B2 (en) 2015-12-08
WO2007075967A3 (en) 2008-01-03
EP1974057A2 (en) 2008-10-01
EP1974057B1 (en) 2012-06-13
WO2007075967A2 (en) 2007-07-05
CA2631316C (en) 2012-12-04
CN101346472A (zh) 2009-01-14
JP5368108B2 (ja) 2013-12-18
WO2007075967B1 (en) 2008-02-21
JP2009521223A (ja) 2009-06-04
ES2388722T3 (es) 2012-10-18
CA2631316A1 (en) 2007-07-05
HK1124640A1 (en) 2009-07-17

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