JP5223267B2 - 遺伝子増幅産物の回収方法および遺伝子増幅産物の回収キット - Google Patents
遺伝子増幅産物の回収方法および遺伝子増幅産物の回収キット Download PDFInfo
- Publication number
- JP5223267B2 JP5223267B2 JP2007216911A JP2007216911A JP5223267B2 JP 5223267 B2 JP5223267 B2 JP 5223267B2 JP 2007216911 A JP2007216911 A JP 2007216911A JP 2007216911 A JP2007216911 A JP 2007216911A JP 5223267 B2 JP5223267 B2 JP 5223267B2
- Authority
- JP
- Japan
- Prior art keywords
- tag sequence
- sequence
- polynucleotide
- recovery
- gene amplification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title claims description 46
- 230000004544 DNA amplification Effects 0.000 title claims description 31
- 238000011027 product recovery Methods 0.000 title claims description 10
- 108091033319 polynucleotide Proteins 0.000 claims description 142
- 102000040430 polynucleotide Human genes 0.000 claims description 142
- 239000002157 polynucleotide Substances 0.000 claims description 142
- 238000011084 recovery Methods 0.000 claims description 66
- 108091081021 Sense strand Proteins 0.000 claims description 45
- 230000000295 complement effect Effects 0.000 claims description 44
- 230000000692 anti-sense effect Effects 0.000 claims description 35
- 150000007523 nucleic acids Chemical class 0.000 claims description 28
- 102000039446 nucleic acids Human genes 0.000 claims description 27
- 108020004707 nucleic acids Proteins 0.000 claims description 27
- 230000002441 reversible effect Effects 0.000 claims description 23
- 239000000126 substance Substances 0.000 claims description 10
- 238000006243 chemical reaction Methods 0.000 description 31
- 239000000047 product Substances 0.000 description 21
- 239000000243 solution Substances 0.000 description 21
- 108020004414 DNA Proteins 0.000 description 19
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 18
- 230000027455 binding Effects 0.000 description 11
- 239000003153 chemical reaction reagent Substances 0.000 description 10
- 238000009396 hybridization Methods 0.000 description 10
- 239000011616 biotin Substances 0.000 description 9
- 229960002685 biotin Drugs 0.000 description 9
- 235000020958 biotin Nutrition 0.000 description 9
- 239000000758 substrate Substances 0.000 description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- 230000003321 amplification Effects 0.000 description 7
- 238000003199 nucleic acid amplification method Methods 0.000 description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000010419 fine particle Substances 0.000 description 6
- 239000002773 nucleotide Substances 0.000 description 6
- 125000003729 nucleotide group Chemical group 0.000 description 6
- 239000000872 buffer Substances 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 238000006911 enzymatic reaction Methods 0.000 description 5
- 238000002372 labelling Methods 0.000 description 5
- LMDZBCPBFSXMTL-UHFFFAOYSA-N 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide Chemical compound CCN=C=NCCCN(C)C LMDZBCPBFSXMTL-UHFFFAOYSA-N 0.000 description 4
- 238000000018 DNA microarray Methods 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 238000001914 filtration Methods 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 4
- 239000004926 polymethyl methacrylate Substances 0.000 description 4
- 239000000523 sample Substances 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 4
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 3
- 108010090804 Streptavidin Proteins 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 238000001962 electrophoresis Methods 0.000 description 3
- 239000000499 gel Substances 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 230000003100 immobilizing effect Effects 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 239000001226 triphosphate Substances 0.000 description 3
- 235000011178 triphosphate Nutrition 0.000 description 3
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- OAKPWEUQDVLTCN-NKWVEPMBSA-N 2',3'-Dideoxyadenosine-5-triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1CC[C@@H](CO[P@@](O)(=O)O[P@](O)(=O)OP(O)(O)=O)O1 OAKPWEUQDVLTCN-NKWVEPMBSA-N 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- OTXOHOIOFJSIFX-POYBYMJQSA-N [[(2s,5r)-5-(2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(=O)O)CC[C@@H]1N1C(=O)NC(=O)C=C1 OTXOHOIOFJSIFX-POYBYMJQSA-N 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 125000003277 amino group Chemical group 0.000 description 2
- 238000000137 annealing Methods 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 239000007979 citrate buffer Substances 0.000 description 2
- 239000006059 cover glass Substances 0.000 description 2
- SPTYHKZRPFATHJ-HYZXJONISA-N dT6 Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)O[C@@H]2[C@H](O[C@H](C2)N2C(NC(=O)C(C)=C2)=O)COP(O)(=O)O[C@@H]2[C@H](O[C@H](C2)N2C(NC(=O)C(C)=C2)=O)COP(O)(=O)O[C@@H]2[C@H](O[C@H](C2)N2C(NC(=O)C(C)=C2)=O)COP(O)(=O)O[C@@H]2[C@H](O[C@H](C2)N2C(NC(=O)C(C)=C2)=O)COP(O)(=O)O[C@@H]2[C@H](O[C@H](C2)N2C(NC(=O)C(C)=C2)=O)CO)[C@@H](O)C1 SPTYHKZRPFATHJ-HYZXJONISA-N 0.000 description 2
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 2
- 229960005542 ethidium bromide Drugs 0.000 description 2
- 238000002523 gelfiltration Methods 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- 150000008300 phosphoramidites Chemical class 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000003252 repetitive effect Effects 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
- 229960000999 sodium citrate dihydrate Drugs 0.000 description 2
- 229940113082 thymine Drugs 0.000 description 2
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 241000972773 Aulopiformes Species 0.000 description 1
- 108090001008 Avidin Proteins 0.000 description 1
- 235000005956 Cosmos caudatus Nutrition 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- HDRRAMINWIWTNU-NTSWFWBYSA-N [[(2s,5r)-5-(2-amino-6-oxo-3h-purin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate Chemical compound C1=2NC(N)=NC(=O)C=2N=CN1[C@H]1CC[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HDRRAMINWIWTNU-NTSWFWBYSA-N 0.000 description 1
- ARLKCWCREKRROD-POYBYMJQSA-N [[(2s,5r)-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)CC1 ARLKCWCREKRROD-POYBYMJQSA-N 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 150000001732 carboxylic acid derivatives Chemical class 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- 239000006249 magnetic particle Substances 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000002923 metal particle Substances 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000000123 paper Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- UQDJGEHQDNVPGU-UHFFFAOYSA-N serine phosphoethanolamine Chemical compound [NH3+]CCOP([O-])(=O)OCC([NH3+])C([O-])=O UQDJGEHQDNVPGU-UHFFFAOYSA-N 0.000 description 1
- 239000011856 silicon-based particle Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2007216911A JP5223267B2 (ja) | 2007-08-23 | 2007-08-23 | 遺伝子増幅産物の回収方法および遺伝子増幅産物の回収キット |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2007216911A JP5223267B2 (ja) | 2007-08-23 | 2007-08-23 | 遺伝子増幅産物の回収方法および遺伝子増幅産物の回収キット |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2009050161A JP2009050161A (ja) | 2009-03-12 |
JP2009050161A5 JP2009050161A5 (enrdf_load_stackoverflow) | 2010-10-07 |
JP5223267B2 true JP5223267B2 (ja) | 2013-06-26 |
Family
ID=40501837
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2007216911A Expired - Fee Related JP5223267B2 (ja) | 2007-08-23 | 2007-08-23 | 遺伝子増幅産物の回収方法および遺伝子増幅産物の回収キット |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP5223267B2 (enrdf_load_stackoverflow) |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IT1303767B1 (it) * | 1998-11-17 | 2001-02-23 | San Raffaele Centro Fond | Metodo di quantificazione di acidi nucleici. |
JP2006075126A (ja) * | 2004-09-13 | 2006-03-23 | National Institute Of Advanced Industrial & Technology | 一塩基変異を検出する方法および検出用プローブ |
-
2007
- 2007-08-23 JP JP2007216911A patent/JP5223267B2/ja not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
JP2009050161A (ja) | 2009-03-12 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP1713936B1 (en) | Genetic analysis by sequence-specific sorting | |
CA2810931C (en) | Direct capture, amplification and sequencing of target dna using immobilized primers | |
CN115176028A (zh) | 用于检测多种分析物的系统和方法 | |
KR102843262B1 (ko) | 자가-발광을 기초로 하는 단일-채널 시퀀싱 방법 | |
KR20010085860A (ko) | 핵산 증폭과 서열분석 방법 | |
CN112840035B (zh) | 对多核苷酸进行测序的方法 | |
JP2013535986A (ja) | 核酸の捕捉法および配列決定法 | |
US20020048760A1 (en) | Use of mismatch cleavage to detect complementary probes | |
EP2173906A1 (en) | High multiplex nucleic acid amplification | |
EP1135528A2 (en) | Length determination of nucleic acid repeat sequences by discontinuous primer extension | |
WO2021031109A1 (zh) | 一种基于发光标记物光信号动力学及二次发光信号对多核苷酸进行测序的方法 | |
JP2005536998A (ja) | 高親和性および高特異性を組み合わせた固相ベースの核酸アッセイ | |
JP2004502468A (ja) | バイオチップ上のヌクレオチド標的配列の同定及び/又は定量のための反転検出 | |
JP5223267B2 (ja) | 遺伝子増幅産物の回収方法および遺伝子増幅産物の回収キット | |
JP2008048705A (ja) | 塩基配列の分析方法及び塩基配列分析用プライマー | |
JP2006141210A (ja) | キメラ遺伝子の検出方法 | |
JP2010035451A (ja) | タグ配列、タグ配列固定化バイオチップおよびタグ配列を用いた選択結合性物質の検出方法 | |
JP2002360247A (ja) | 核酸固定化物 | |
JP2007300829A (ja) | Dnaマイクロアレイ等に供する検体の調製方法 | |
RU2794177C1 (ru) | Способ одноканального секвенирования на основе самолюминесценции | |
JP5266646B2 (ja) | 繰り返し塩基配列の配列数測定方法および繰り返し塩基配列の配列数測定キット | |
KR102013786B1 (ko) | cDNA 또는 앰플리콘 및 이를 담지한 담체를 포함하는 구조체 | |
WO2024098185A1 (en) | Nucleic acid sequencing using self-luminescence | |
JP2010142251A (ja) | 検出核酸用プローブセット | |
JP2007295855A (ja) | 核酸修飾解析のためのサンプル核酸の製造方法と、当該サンプル核酸を用いた核酸修飾を検出する方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A521 | Written amendment |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20100820 |
|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20100820 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20121120 |
|
A521 | Written amendment |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20130107 |
|
RD03 | Notification of appointment of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7423 Effective date: 20130107 |
|
A521 | Written amendment |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20130107 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20130212 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20130225 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20160322 Year of fee payment: 3 |
|
LAPS | Cancellation because of no payment of annual fees |