JP5162095B2 - マイクロチップ、核酸抽出用のキット及び核酸の抽出方法 - Google Patents

マイクロチップ、核酸抽出用のキット及び核酸の抽出方法 Download PDF

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Publication number
JP5162095B2
JP5162095B2 JP2005504072A JP2005504072A JP5162095B2 JP 5162095 B2 JP5162095 B2 JP 5162095B2 JP 2005504072 A JP2005504072 A JP 2005504072A JP 2005504072 A JP2005504072 A JP 2005504072A JP 5162095 B2 JP5162095 B2 JP 5162095B2
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nucleic acid
microbeads
microchip
acid extraction
fine channel
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Japanese (ja)
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JPWO2004086055A1 (ja
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通博 大西
圭祐 森島
武彦 北森
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Kanagawa Academy of Science and Technology
Sony Corp
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Kanagawa Academy of Science and Technology
Sony Corp
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00783—Laminate assemblies, i.e. the reactor comprising a stack of plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
JP2005504072A 2003-03-24 2004-03-24 マイクロチップ、核酸抽出用のキット及び核酸の抽出方法 Expired - Fee Related JP5162095B2 (ja)

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JP2005504072A JP5162095B2 (ja) 2003-03-24 2004-03-24 マイクロチップ、核酸抽出用のキット及び核酸の抽出方法

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2003081605 2003-03-24
JP2003081605 2003-03-24
JP2005504072A JP5162095B2 (ja) 2003-03-24 2004-03-24 マイクロチップ、核酸抽出用のキット及び核酸の抽出方法
PCT/JP2004/003998 WO2004086055A1 (ja) 2003-03-24 2004-03-24 マイクロチップ、核酸抽出用のキット及び核酸の抽出方法

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JPWO2004086055A1 JPWO2004086055A1 (ja) 2006-06-29
JP5162095B2 true JP5162095B2 (ja) 2013-03-13

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US (1) US8012680B2 (US20060234241A1-20061019-C00001.png)
EP (1) EP1607748B1 (US20060234241A1-20061019-C00001.png)
JP (1) JP5162095B2 (US20060234241A1-20061019-C00001.png)
KR (1) KR20050111785A (US20060234241A1-20061019-C00001.png)
CN (1) CN1764842B (US20060234241A1-20061019-C00001.png)
WO (1) WO2004086055A1 (US20060234241A1-20061019-C00001.png)

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JP2006029798A (ja) * 2004-07-12 2006-02-02 Hitachi Software Eng Co Ltd 試薬を内蔵した高反応効率生体物質検査チップ
US20090149345A1 (en) * 2005-03-07 2009-06-11 Kuraray Co., Ltd. Microchannel array and method for producing the same, and blood measuring method employing it
JP4904973B2 (ja) * 2005-08-08 2012-03-28 ソニー株式会社 核酸回収方法、核酸回収装置、並びに核酸供給装置
JP4657084B2 (ja) * 2005-11-02 2011-03-23 パナソニック株式会社 試料分析用ディスク
JP2007147456A (ja) * 2005-11-28 2007-06-14 Seiko Epson Corp マイクロ流体システム、試料分析装置、及び標的物質の検出または測定方法
US7608399B2 (en) 2006-06-26 2009-10-27 Blood Cell Storage, Inc. Device and method for extraction and analysis of nucleic acids from biological samples
JP4848896B2 (ja) * 2006-08-31 2011-12-28 横河電機株式会社 マイクロチップ
JP4297148B2 (ja) 2006-09-22 2009-07-15 ソニー株式会社 核酸回収装置及び核酸回収方法
WO2009117167A1 (en) 2008-01-02 2009-09-24 Blood Cell Storage, Inc. Devices and processes for nucleic acid extraction
KR100945129B1 (ko) * 2008-03-17 2010-03-02 고정문 디앤에이 정제용 칩 및 이를 이용한 정제 방법
WO2009150583A1 (en) * 2008-06-10 2009-12-17 Koninklijke Philips Electronics N.V. Diagnostic device
KR101563687B1 (ko) 2008-09-02 2015-11-09 삼성전자주식회사 표적 분자 분리를 위한 미세유동 카트리지, 분리장치, 및 이를 이용한 표적 분자 분리 방법
CN103398936A (zh) * 2009-07-07 2013-11-20 索尼公司 微流体装置
WO2012159063A2 (en) 2011-05-19 2012-11-22 Blood Cell Strorage, Inc. Gravity flow fluidic device for nucleic acid extraction
KR101428578B1 (ko) * 2012-05-21 2014-08-12 한국과학기술원 미소입자 선택적 포획-회수 장치
CN108031457B (zh) * 2017-12-22 2020-11-17 深圳逗点生物技术有限公司 一种吸附核酸的复合材料及其制备方法、吸附核酸的装置
IL263582A (en) 2018-12-09 2020-06-30 Vbact Ltd A system for photographing and monitoring flows

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WO2001032930A1 (en) * 1999-11-04 2001-05-10 California Institute Of Technology Methods and apparatuses for analyzing polynucleotide sequences
WO2001038865A1 (en) * 1999-11-26 2001-05-31 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems
JP3220158B2 (ja) * 1994-11-14 2001-10-22 トラスティーズ・オブ・ザ・ユニバーシティ・オブ・ペンシルベニア 分析対象物質の確定及び処理のためのメソ規模の試料前処理装置及びシステム
JP2002503331A (ja) * 1995-12-05 2002-01-29 ガメラ バイオサイエンス コーポレイション 機内に搭載された情報科学を備えた超微量液体素子工学システムにおいて液体移動を推進するために求心性加速を使用するための装置及び方法
JP2002532715A (ja) * 1998-12-16 2002-10-02 キュラゲン コーポレイション 空間的且つ時間的に変動する電場による荷電粒子の分離

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JP3220158B2 (ja) * 1994-11-14 2001-10-22 トラスティーズ・オブ・ザ・ユニバーシティ・オブ・ペンシルベニア 分析対象物質の確定及び処理のためのメソ規模の試料前処理装置及びシステム
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JP2002532715A (ja) * 1998-12-16 2002-10-02 キュラゲン コーポレイション 空間的且つ時間的に変動する電場による荷電粒子の分離
WO2001032930A1 (en) * 1999-11-04 2001-05-10 California Institute Of Technology Methods and apparatuses for analyzing polynucleotide sequences
WO2001038865A1 (en) * 1999-11-26 2001-05-31 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems

Also Published As

Publication number Publication date
EP1607748A1 (en) 2005-12-21
CN1764842B (zh) 2012-01-11
US8012680B2 (en) 2011-09-06
JPWO2004086055A1 (ja) 2006-06-29
KR20050111785A (ko) 2005-11-28
EP1607748A4 (en) 2012-05-30
CN1764842A (zh) 2006-04-26
EP1607748B1 (en) 2013-05-15
WO2004086055A1 (ja) 2004-10-07
US20060234241A1 (en) 2006-10-19

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