JP5153943B2 - 抗微生物剤 - Google Patents

抗微生物剤 Download PDF

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JP5153943B2
JP5153943B2 JP2011524360A JP2011524360A JP5153943B2 JP 5153943 B2 JP5153943 B2 JP 5153943B2 JP 2011524360 A JP2011524360 A JP 2011524360A JP 2011524360 A JP2011524360 A JP 2011524360A JP 5153943 B2 JP5153943 B2 JP 5153943B2
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endolysin
seq
modified
variant according
amino acid
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JP2012500642A (ja
JP2012500642A5 (https=
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イベス ブライアーズ
ロブ ラビーニュ
グイド ボルケート
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カトリック ユニバーシテイト ルーベン ケイ．ユー． ルーベン アール アンド ディー
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/02—Local antiseptics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Public Health (AREA)
  • Mycology (AREA)
  • Communicable Diseases (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Oncology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Cosmetics (AREA)
  • Apparatus For Disinfection Or Sterilisation (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2011524360A 2008-08-26 2009-08-25 抗微生物剤 Active JP5153943B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0815484.1A GB0815484D0 (en) 2008-08-26 2008-08-26 Antibacterial agents
GB0815484.1 2008-08-26
PCT/EP2009/060947 WO2010023207A2 (en) 2008-08-26 2009-08-25 Antimicrobial agents

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2012265070A Division JP5571757B2 (ja) 2008-08-26 2012-12-04 抗微生物剤

Publications (3)

Publication Number Publication Date
JP2012500642A JP2012500642A (ja) 2012-01-12
JP2012500642A5 JP2012500642A5 (https=) 2012-07-26
JP5153943B2 true JP5153943B2 (ja) 2013-02-27

Family

ID=39846783

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2011524360A Active JP5153943B2 (ja) 2008-08-26 2009-08-25 抗微生物剤
JP2012265070A Active JP5571757B2 (ja) 2008-08-26 2012-12-04 抗微生物剤

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2012265070A Active JP5571757B2 (ja) 2008-08-26 2012-12-04 抗微生物剤

Country Status (16)

Country Link
US (1) US9809808B2 (https=)
EP (1) EP2331687B1 (https=)
JP (2) JP5153943B2 (https=)
KR (1) KR101473271B1 (https=)
CN (1) CN102197132B (https=)
AU (1) AU2009286760B2 (https=)
BR (1) BRPI0917508A2 (https=)
CA (1) CA2735077C (https=)
DK (1) DK2331687T3 (https=)
EA (1) EA024794B1 (https=)
ES (1) ES2395357T3 (https=)
GB (1) GB0815484D0 (https=)
IL (1) IL211441A (https=)
MX (1) MX2011001957A (https=)
PL (1) PL2331687T3 (https=)
WO (1) WO2010023207A2 (https=)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0815484D0 (en) 2008-08-26 2008-10-01 Univ Leuven Kath Antibacterial agents
AU2010264659B2 (en) * 2009-06-26 2016-08-04 Lysando Aktiengesellschaft Antimicrobial agents
PL2445515T3 (pl) 2009-06-26 2016-10-31 Środki przeciwdrobnoustrojowe
KR20120059576A (ko) * 2009-08-24 2012-06-08 뤼산도 홀딩 아게 신규한 엔도리신 ＯＢＰｇｐＬＹＳ
SG184836A1 (en) * 2010-04-27 2012-11-29 Lysando Ag Method of reducing biofilms
CA3048783C (en) 2010-09-17 2022-05-24 Tecnifar-Industria Tecnica Farmaceutica, S.A. Antibacterial phage, phage peptides and methods of use thereof
GB201018518D0 (en) * 2010-11-03 2010-12-15 Univ Leuven Kath Novel endolysin
EP2468856A1 (en) * 2010-12-23 2012-06-27 Lysando Aktiengesellschaft Antimicrobial Agents
DK2699253T3 (en) * 2011-04-21 2018-07-16 Univ Rockefeller STREPTOCOCCUS BACTERYPHAGIC LYSINES FOR THE DETECTION AND TREATMENT OF GRAM POSITIVE BACTERIES
EP2702070B1 (en) * 2011-04-27 2015-09-30 Lysando AG New antimicrobial agents
US9068204B2 (en) 2013-03-15 2015-06-30 The United States Of America, As Represented By The Secretary Of Agriculture Peptidoglycan hydrolase antimicrobials for eradicating lactobacilli that contaminate and reduce ethanol yields in biofuel fermentation
WO2015070912A1 (en) 2013-11-14 2015-05-21 Lysando Ag Modified el188 endolysin sequence
WO2015070911A1 (en) * 2013-11-14 2015-05-21 Lysando Ag Modified kz144 endolysin sequence
EP3105322B1 (en) 2014-02-14 2018-12-12 Lysando AG Antimicrobial agents
US20170106058A1 (en) * 2014-04-08 2017-04-20 Lysando Ag Pharmaceutical composition against chronic bacterial infections
WO2015200783A2 (en) 2014-06-26 2015-12-30 The Rockefeller University Acinetobacter lysins
CN104762285B (zh) * 2015-04-23 2017-10-27 武汉菲吉乐科生物科技有限公司 一种自内裂解大肠杆菌的裂解酶及其应用
CN110300760B (zh) * 2016-12-16 2023-10-20 米尼奥大学 新的内溶素
MX2019011808A (es) * 2017-04-03 2020-01-09 Sasinapas Co Ltd Endolisinas de gram-negativas modificadas.
WO2019118632A1 (en) * 2017-12-12 2019-06-20 Contrafect Corporation Identification of lysins and derivatives thereof with bacterial activity against pseudomonas aeruginosa
AU2019276253A1 (en) 2018-05-30 2020-11-26 Lysando Ag Novel antimicrobial proteins
WO2019229184A1 (en) 2018-05-30 2019-12-05 Lysando Ag Novel antimicrobial fusion proteins
MX2021013491A (es) * 2019-05-08 2022-02-21 Phagomed Biopharma Gmbh Nuevas endolisinas de gardnerella y usos de las mismas.
WO2023225518A2 (en) * 2022-05-16 2023-11-23 The Broad Institute, Inc. Engineered pnma proteins and delivery systems thereof
CN117737001B (zh) * 2022-12-27 2024-08-09 菲吉乐科(南京)生物科技有限公司 一种防治犬细菌性腹泻的大肠杆菌噬菌体及其应用
CN118620800B (zh) * 2024-08-08 2024-11-12 中国兽医药品监察所 一株鼻疽伯克霍尔德氏菌及其应用

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR970006154B1 (ko) 1987-07-06 1997-04-24 루이지아나 스테이트 유니버시티 아그리컬춰럴 앤드 메카니칼 컬리지 용해성 펩티드에 의한 진핵 병원체와 신생물의 억제 및 섬유아세포와 임파구의 자극
GB2255561B (en) 1991-04-20 1995-06-21 Agricultural & Food Res Lysins from bacteriophages
EP0656952B1 (en) 1992-08-21 2003-03-05 The University Of British Columbia Cationic peptides and method for production
ATE198119T1 (de) 1994-09-01 2001-01-15 Novo Nordisk As Eine grundlegende proteinzusammensetzung zur abtötung oder inhibierung mikrobieller zellen
US6503881B2 (en) 1996-08-21 2003-01-07 Micrologix Biotech Inc. Compositions and methods for treating infections using cationic peptides alone or in combination with antibiotics
US6428784B1 (en) 1997-10-31 2002-08-06 New Horizons Diagnostics Corp Vaginal suppository for treating group B Streptococcus infection
US5997862A (en) * 1997-10-31 1999-12-07 New Horizons Diagnostics Corporation Therapeutic treatment of group A streptococcal infections
US6288212B1 (en) 1998-08-28 2001-09-11 The University Of British Columbia Anti-endotoxic, antimicrobial cationic peptides and methods of use therefor
US5993809A (en) 1998-11-18 1999-11-30 Children's Hospital Medical Center Lysozyme fusion proteins in infections
WO2001000855A1 (en) 1999-06-23 2001-01-04 Ppl Therapeutics (Scotland) Ltd. Fusion proteins incorporating lysozyme
ATE443767T1 (de) 2002-04-22 2009-10-15 Dow Global Technologies Inc Kostengünstige herstellung von peptiden
US7566447B2 (en) 2003-05-15 2009-07-28 Iogenetics, Llc Biocides
BRPI0413833A (pt) 2003-09-11 2006-10-24 Novozymes As célula hospedeira microbiana recombinante, construção de ácido nucleico, método para produzir uma enzima e/ou um agente antimicrobiano, produto de fusão, aditivo e composição de ração animal, animal não humano transgênico ou produtos ou elementos deste, usos do produto de fusão, da co-expressão de um agente antimicrobiano e uma enzima, e de um peptìdeo de proteção, e, método para identificar um peptìdeo de proteção
WO2005108563A2 (en) 2004-04-19 2005-11-17 University Of Chicago Peptidoglycan-hydrolyzing protein encoded by bacteriophage n4
US7572602B1 (en) 2004-12-03 2009-08-11 The United States Of America As Represented By The Secretary Of Agriculture Nucleic acid encoding endolysin fusion protein
DE102005040347A1 (de) 2005-08-25 2007-03-01 Profos Ag Verfahren und Mittel zur Anreicherung, Entfernung und zum Nachweis von Listerien
ATE470451T1 (de) 2006-03-31 2010-06-15 Univ St Andrews Antimikrobielle zusammensetzungen mit einem kationischen peptid und glycylglycin- endopeptidase
DE102006061002A1 (de) 2006-12-22 2008-06-26 Profos Ag Verfahren und Mittel zur Anreicherung, Entfernung und zum Nachweis von gram-positiven Bakterien
CN107129524B (zh) 2007-09-25 2023-11-21 田园温室气体研究有限公司 用于抑制微生物细胞的疫苗和疫苗组分
JP2011504366A (ja) 2007-11-26 2011-02-10 プラント バイオサイエンス リミティド エンドリシン活性を有する新規ポリペプチド及びその使用
WO2010011960A2 (en) 2008-07-24 2010-01-28 The United State Of America, As Represented By The Secretary Of Agriculture Triple acting antimicrobials that are refractory to resistance development
EP2157100A1 (en) 2008-08-19 2010-02-24 Profos AG Artificial peptidoglycan lysing enzymes and peptidoglycan binding proteins
GB0815484D0 (en) 2008-08-26 2008-10-01 Univ Leuven Kath Antibacterial agents
US8389679B2 (en) 2009-02-05 2013-03-05 The Regents Of The University Of California Targeted antimicrobial moieties
PL2445515T3 (pl) 2009-06-26 2016-10-31 Środki przeciwdrobnoustrojowe
KR20120059576A (ko) 2009-08-24 2012-06-08 뤼산도 홀딩 아게 신규한 엔도리신 ＯＢＰｇｐＬＹＳ
SG184836A1 (en) 2010-04-27 2012-11-29 Lysando Ag Method of reducing biofilms

Also Published As

Publication number Publication date
CN102197132B (zh) 2016-01-13
DK2331687T3 (da) 2013-01-07
MX2011001957A (es) 2011-06-20
BRPI0917508A2 (pt) 2015-08-04
KR20110049883A (ko) 2011-05-12
AU2009286760A1 (en) 2010-03-04
AU2009286760B2 (en) 2015-08-27
CA2735077A1 (en) 2010-03-04
EP2331687A2 (en) 2011-06-15
HK1157818A1 (en) 2012-07-06
KR101473271B1 (ko) 2014-12-16
US20110243915A1 (en) 2011-10-06
CA2735077C (en) 2019-04-09
JP2012500642A (ja) 2012-01-12
ES2395357T3 (es) 2013-02-12
EP2331687B1 (en) 2012-09-19
WO2010023207A3 (en) 2010-07-15
JP5571757B2 (ja) 2014-08-13
JP2013081465A (ja) 2013-05-09
EA024794B1 (ru) 2016-10-31
CN102197132A (zh) 2011-09-21
PL2331687T3 (pl) 2013-02-28
WO2010023207A2 (en) 2010-03-04
IL211441A (en) 2015-04-30
US9809808B2 (en) 2017-11-07
EA201100312A1 (ru) 2011-10-31
GB0815484D0 (en) 2008-10-01

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