CA2735077C - Antimicrobial agents - Google Patents

Antimicrobial agents Download PDF

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Publication number
CA2735077C
CA2735077C CA2735077A CA2735077A CA2735077C CA 2735077 C CA2735077 C CA 2735077C CA 2735077 A CA2735077 A CA 2735077A CA 2735077 A CA2735077 A CA 2735077A CA 2735077 C CA2735077 C CA 2735077C
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CA
Canada
Prior art keywords
endolysin
seq
gram
amino acid
modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2735077A
Other languages
English (en)
French (fr)
Other versions
CA2735077A1 (en
Inventor
Yves Briers
Rob Lavigne
Guido Volckaert
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Katholieke Universiteit Leuven
Original Assignee
Katholieke Universiteit Leuven
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Katholieke Universiteit Leuven filed Critical Katholieke Universiteit Leuven
Publication of CA2735077A1 publication Critical patent/CA2735077A1/en
Application granted granted Critical
Publication of CA2735077C publication Critical patent/CA2735077C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/02—Local antiseptics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Public Health (AREA)
  • Mycology (AREA)
  • Communicable Diseases (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Oncology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Cosmetics (AREA)
  • Apparatus For Disinfection Or Sterilisation (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
CA2735077A 2008-08-26 2009-08-25 Antimicrobial agents Active CA2735077C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0815484.1A GB0815484D0 (en) 2008-08-26 2008-08-26 Antibacterial agents
GB0815484.1 2008-08-26
PCT/EP2009/060947 WO2010023207A2 (en) 2008-08-26 2009-08-25 Antimicrobial agents

Publications (2)

Publication Number Publication Date
CA2735077A1 CA2735077A1 (en) 2010-03-04
CA2735077C true CA2735077C (en) 2019-04-09

Family

ID=39846783

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2735077A Active CA2735077C (en) 2008-08-26 2009-08-25 Antimicrobial agents

Country Status (16)

Country Link
US (1) US9809808B2 (https=)
EP (1) EP2331687B1 (https=)
JP (2) JP5153943B2 (https=)
KR (1) KR101473271B1 (https=)
CN (1) CN102197132B (https=)
AU (1) AU2009286760B2 (https=)
BR (1) BRPI0917508A2 (https=)
CA (1) CA2735077C (https=)
DK (1) DK2331687T3 (https=)
EA (1) EA024794B1 (https=)
ES (1) ES2395357T3 (https=)
GB (1) GB0815484D0 (https=)
IL (1) IL211441A (https=)
MX (1) MX2011001957A (https=)
PL (1) PL2331687T3 (https=)
WO (1) WO2010023207A2 (https=)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0815484D0 (en) 2008-08-26 2008-10-01 Univ Leuven Kath Antibacterial agents
DK2445515T3 (en) 2009-06-26 2016-07-04 Univ Leuven Kath antimicrobial agents
KR101701890B1 (ko) * 2009-06-26 2017-02-02 뤼산도 아게 항균제
SG178440A1 (en) 2009-08-24 2012-04-27 Univ Leuven Kath New endolysin obpgplys
EA037276B1 (ru) * 2010-04-27 2021-03-03 Лисандо Аг Способы устранения или уменьшения бактериальной биопленки
SG188566A1 (en) 2010-09-17 2013-04-30 Tecnifar Ind Tecnica Farmaceutica S A Antibacterial phage, phage peptides and methods of use thereof
GB201018518D0 (en) 2010-11-03 2010-12-15 Univ Leuven Kath Novel endolysin
EP2468856A1 (en) * 2010-12-23 2012-06-27 Lysando Aktiengesellschaft Antimicrobial Agents
CA2833409A1 (en) * 2011-04-21 2012-10-26 The Rockefeller University Streptococcus bacteriophage lysins for detection and treatment of gram positive bacteria
US20140120074A1 (en) * 2011-04-27 2014-05-01 Lysando Ag Antimicrobial agents
US9068204B2 (en) 2013-03-15 2015-06-30 The United States Of America, As Represented By The Secretary Of Agriculture Peptidoglycan hydrolase antimicrobials for eradicating lactobacilli that contaminate and reduce ethanol yields in biofuel fermentation
WO2015070912A1 (en) 2013-11-14 2015-05-21 Lysando Ag Modified el188 endolysin sequence
WO2015070911A1 (en) * 2013-11-14 2015-05-21 Lysando Ag Modified kz144 endolysin sequence
WO2015121443A1 (en) 2014-02-14 2015-08-20 Lysando Ag Antimicrobial agents
CA2941426C (en) * 2014-04-08 2022-09-27 Lysando Ag Pharmaceutical composition against chronic bacterial infections
AU2015279705B2 (en) * 2014-06-26 2021-04-01 The Rockefeller University Acinetobacter lysins
CN104762285B (zh) * 2015-04-23 2017-10-27 武汉菲吉乐科生物科技有限公司 一种自内裂解大肠杆菌的裂解酶及其应用
AU2017375050B2 (en) * 2016-12-16 2021-07-22 Universidade Do Minho Novel endolysin
AU2018248060B2 (en) 2017-04-03 2024-05-23 Sasinapas Co., Ltd. Engineered Gram-negative endolysins
BR112020011433A2 (pt) * 2017-12-12 2020-12-01 Contrafect Corporation identificação de lisinas e seus derivados com atividade bacteriana contra pseudomonas aeruginosa
EP3802620A1 (en) 2018-05-30 2021-04-14 Lysando AG Novel antimicrobial fusion proteins
US11958890B2 (en) 2018-05-30 2024-04-16 Lysando Ag Antimicrobial proteins
CN120136980A (zh) * 2019-05-08 2025-06-13 生物技术公司 新的加德纳菌内溶素及其用途
WO2023225518A2 (en) * 2022-05-16 2023-11-23 The Broad Institute, Inc. Engineered pnma proteins and delivery systems thereof
CN117737001B (zh) * 2022-12-27 2024-08-09 菲吉乐科(南京)生物科技有限公司 一种防治犬细菌性腹泻的大肠杆菌噬菌体及其应用
CN118620800B (zh) * 2024-08-08 2024-11-12 中国兽医药品监察所 一株鼻疽伯克霍尔德氏菌及其应用

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989000194A1 (en) 1987-07-06 1989-01-12 Louisiana State University Agricultural And Mechan Inhibition of eucaryotic pathogens and neoplasms and stimulation of fibroblasts and lymphocytes with lytic peptides
GB2255561B (en) 1991-04-20 1995-06-21 Agricultural & Food Res Lysins from bacteriophages
ATE233817T1 (de) 1992-08-21 2003-03-15 Univ British Columbia Kationische peptide und verfahren zu deren herstellung
ATE198119T1 (de) 1994-09-01 2001-01-15 Novo Nordisk As Eine grundlegende proteinzusammensetzung zur abtötung oder inhibierung mikrobieller zellen
US6503881B2 (en) 1996-08-21 2003-01-07 Micrologix Biotech Inc. Compositions and methods for treating infections using cationic peptides alone or in combination with antibiotics
US6428784B1 (en) * 1997-10-31 2002-08-06 New Horizons Diagnostics Corp Vaginal suppository for treating group B Streptococcus infection
US5997862A (en) * 1997-10-31 1999-12-07 New Horizons Diagnostics Corporation Therapeutic treatment of group A streptococcal infections
US6288212B1 (en) 1998-08-28 2001-09-11 The University Of British Columbia Anti-endotoxic, antimicrobial cationic peptides and methods of use therefor
US5993809A (en) 1998-11-18 1999-11-30 Children's Hospital Medical Center Lysozyme fusion proteins in infections
EP1194570A1 (en) 1999-06-23 2002-04-10 PPL Therapeutics (Scotland) Limited Fusion proteins incorporating lysozyme
CA2482995C (en) 2002-04-22 2013-01-29 Dow Global Technologies Inc. Low-cost production of peptides
US7566447B2 (en) 2003-05-15 2009-07-28 Iogenetics, Llc Biocides
JP2007504810A (ja) 2003-09-11 2007-03-08 ノボザイムス アクティーゼルスカブ 抗微生物剤の組換え製造
WO2005108563A2 (en) 2004-04-19 2005-11-17 University Of Chicago Peptidoglycan-hydrolyzing protein encoded by bacteriophage n4
US7572602B1 (en) 2004-12-03 2009-08-11 The United States Of America As Represented By The Secretary Of Agriculture Nucleic acid encoding endolysin fusion protein
DE102005040347A1 (de) 2005-08-25 2007-03-01 Profos Ag Verfahren und Mittel zur Anreicherung, Entfernung und zum Nachweis von Listerien
US7943128B2 (en) 2006-03-31 2011-05-17 The University Court Of The University Of St Andrews Anti-microbial compositions comprising a cationic peptide and a glycylglycine endopeptidase
DE102006061002A1 (de) 2006-12-22 2008-06-26 Profos Ag Verfahren und Mittel zur Anreicherung, Entfernung und zum Nachweis von gram-positiven Bakterien
US8586709B2 (en) 2007-09-25 2013-11-19 Pastoral Greenhouse Research Limited Cell-permeabilising peptides and polypeptides for microbial cells
AU2008328584A1 (en) 2007-11-26 2009-06-04 Plant Bioscience Limited Novel polypeptides having endolysin activity and uses thereof
CA2736062A1 (en) 2008-07-24 2010-01-28 The United State Of America, As Represented By The Secretary Of Agricuure Triple acting antimicrobials that are refractory to resistance development
EP2157100A1 (en) 2008-08-19 2010-02-24 Profos AG Artificial peptidoglycan lysing enzymes and peptidoglycan binding proteins
GB0815484D0 (en) 2008-08-26 2008-10-01 Univ Leuven Kath Antibacterial agents
WO2010091294A2 (en) 2009-02-05 2010-08-12 The Regents Of The University Of California New targeted antimicrobial moieties
DK2445515T3 (en) 2009-06-26 2016-07-04 Univ Leuven Kath antimicrobial agents
SG178440A1 (en) 2009-08-24 2012-04-27 Univ Leuven Kath New endolysin obpgplys
EA037276B1 (ru) 2010-04-27 2021-03-03 Лисандо Аг Способы устранения или уменьшения бактериальной биопленки

Also Published As

Publication number Publication date
DK2331687T3 (da) 2013-01-07
MX2011001957A (es) 2011-06-20
EA201100312A1 (ru) 2011-10-31
JP5571757B2 (ja) 2014-08-13
WO2010023207A3 (en) 2010-07-15
US9809808B2 (en) 2017-11-07
JP2013081465A (ja) 2013-05-09
ES2395357T3 (es) 2013-02-12
AU2009286760B2 (en) 2015-08-27
WO2010023207A2 (en) 2010-03-04
CN102197132B (zh) 2016-01-13
PL2331687T3 (pl) 2013-02-28
JP5153943B2 (ja) 2013-02-27
AU2009286760A1 (en) 2010-03-04
KR101473271B1 (ko) 2014-12-16
CN102197132A (zh) 2011-09-21
HK1157818A1 (en) 2012-07-06
CA2735077A1 (en) 2010-03-04
EP2331687A2 (en) 2011-06-15
BRPI0917508A2 (pt) 2015-08-04
JP2012500642A (ja) 2012-01-12
GB0815484D0 (en) 2008-10-01
EP2331687B1 (en) 2012-09-19
US20110243915A1 (en) 2011-10-06
IL211441A (en) 2015-04-30
EA024794B1 (ru) 2016-10-31
KR20110049883A (ko) 2011-05-12

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