JP5132480B2 - 顕微鏡 - Google Patents
顕微鏡 Download PDFInfo
- Publication number
- JP5132480B2 JP5132480B2 JP2008216873A JP2008216873A JP5132480B2 JP 5132480 B2 JP5132480 B2 JP 5132480B2 JP 2008216873 A JP2008216873 A JP 2008216873A JP 2008216873 A JP2008216873 A JP 2008216873A JP 5132480 B2 JP5132480 B2 JP 5132480B2
- Authority
- JP
- Japan
- Prior art keywords
- objective lens
- phase difference
- difference objective
- microscope
- phase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 230000014509 gene expression Effects 0.000 claims description 29
- 210000001747 pupil Anatomy 0.000 claims description 28
- 238000001514 detection method Methods 0.000 claims description 17
- 239000004065 semiconductor Substances 0.000 claims description 9
- 239000013307 optical fiber Substances 0.000 claims description 4
- 238000003384 imaging method Methods 0.000 claims description 3
- 230000005855 radiation Effects 0.000 claims description 2
- 230000003287 optical effect Effects 0.000 description 13
- 230000000694 effects Effects 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 230000005540 biological transmission Effects 0.000 description 4
- 238000001917 fluorescence detection Methods 0.000 description 4
- 101100501772 Arabidopsis thaliana ESR2 gene Proteins 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 238000001000 micrograph Methods 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000006866 deterioration Effects 0.000 description 1
- 238000002073 fluorescence micrograph Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 201000008111 hereditary spherocytosis type 2 Diseases 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 101150074925 sph2 gene Proteins 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
- G02B21/14—Condensers affording illumination for phase-contrast observation
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0056—Optical details of the image generation based on optical coherence, e.g. phase-contrast arrangements, interference arrangements
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Microscoopes, Condenser (AREA)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2008216873A JP5132480B2 (ja) | 2008-08-26 | 2008-08-26 | 顕微鏡 |
| US12/486,420 US8094371B2 (en) | 2008-08-26 | 2009-06-17 | Microscope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2008216873A JP5132480B2 (ja) | 2008-08-26 | 2008-08-26 | 顕微鏡 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010054601A JP2010054601A (ja) | 2010-03-11 |
| JP2010054601A5 JP2010054601A5 (enExample) | 2011-09-15 |
| JP5132480B2 true JP5132480B2 (ja) | 2013-01-30 |
Family
ID=41725060
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2008216873A Expired - Fee Related JP5132480B2 (ja) | 2008-08-26 | 2008-08-26 | 顕微鏡 |
Country Status (2)
| Country | Link |
|---|---|
| US (1) | US8094371B2 (enExample) |
| JP (1) | JP5132480B2 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012048026A (ja) * | 2010-08-27 | 2012-03-08 | Sony Corp | 顕微鏡及びフィルタ挿入方法 |
| JP5201185B2 (ja) * | 2010-09-14 | 2013-06-05 | オムロン株式会社 | 観察光学系およびレーザ加工装置 |
| WO2013089258A1 (ja) * | 2011-12-15 | 2013-06-20 | 株式会社ニコン | 顕微鏡及び刺激装置 |
| US20130162800A1 (en) * | 2011-12-22 | 2013-06-27 | General Electric Company | Quantitative phase microscopy for label-free high-contrast cell imaging using frequency domain phase shift |
| US9097900B2 (en) * | 2012-06-14 | 2015-08-04 | General Electric Company | Quantitative phase microscopy for high-contrast cell imaging using frequency domain phase shift |
| JP6381437B2 (ja) * | 2014-12-24 | 2018-08-29 | オリンパス株式会社 | 位相差顕微鏡 |
| JP6784773B2 (ja) | 2016-11-17 | 2020-11-11 | オリンパス株式会社 | 標本観察装置 |
| DE102017110638B3 (de) * | 2017-05-16 | 2018-09-27 | Leica Microsystems Cms Gmbh | Mikroskop und Mikroskopbeleuchtungsverfahren |
| JP7112686B2 (ja) * | 2018-07-18 | 2022-08-04 | 株式会社エビデント | 標本観察装置 |
| JP7641528B2 (ja) * | 2021-05-21 | 2025-03-07 | パナソニックIpマネジメント株式会社 | 位相差顕微鏡 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5241364A (en) * | 1990-10-19 | 1993-08-31 | Fuji Photo Film Co., Ltd. | Confocal scanning type of phase contrast microscope and scanning microscope |
| US5751475A (en) * | 1993-12-17 | 1998-05-12 | Olympus Optical Co., Ltd. | Phase contrast microscope |
| JP3708246B2 (ja) * | 1996-09-19 | 2005-10-19 | オリンパス株式会社 | 光制御部材を有する光学顕微鏡 |
| JP3841523B2 (ja) | 1997-09-11 | 2006-11-01 | オリンパス株式会社 | 位相差顕微鏡 |
| JP4222877B2 (ja) | 2003-05-28 | 2009-02-12 | オリンパス株式会社 | 顕微鏡観察方法及びそれに用いる顕微鏡 |
| US7564622B2 (en) | 2003-12-12 | 2009-07-21 | Olympus Corporation | Methods for implement microscopy and microscopic measurement as well as microscope and apparatus for implementing them |
| JP4582762B2 (ja) | 2003-12-12 | 2010-11-17 | オリンパス株式会社 | 顕微鏡観察方法及びそれを用いるための顕微鏡 |
-
2008
- 2008-08-26 JP JP2008216873A patent/JP5132480B2/ja not_active Expired - Fee Related
-
2009
- 2009-06-17 US US12/486,420 patent/US8094371B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JP2010054601A (ja) | 2010-03-11 |
| US20100053740A1 (en) | 2010-03-04 |
| US8094371B2 (en) | 2012-01-10 |
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