JP4986358B2 - ミスマッチエンドヌクレアーゼをコードする核酸分子およびその使用方法 - Google Patents

ミスマッチエンドヌクレアーゼをコードする核酸分子およびその使用方法 Download PDF

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Publication number
JP4986358B2
JP4986358B2 JP2001561782A JP2001561782A JP4986358B2 JP 4986358 B2 JP4986358 B2 JP 4986358B2 JP 2001561782 A JP2001561782 A JP 2001561782A JP 2001561782 A JP2001561782 A JP 2001561782A JP 4986358 B2 JP4986358 B2 JP 4986358B2
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Prior art keywords
cel
sequence
nucleic acid
dna
seq
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JP2001561782A
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Japanese (ja)
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JP2004529602A5 (enExample
JP2004529602A (ja
Inventor
アンソニー・ティ・ユング
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Fox Chase Cancer Center
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Fox Chase Cancer Center
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Priority claimed from US09/510,322 external-priority patent/US6391557B1/en
Priority claimed from US09/514,768 external-priority patent/US6699980B1/en
Application filed by Fox Chase Cancer Center filed Critical Fox Chase Cancer Center
Publication of JP2004529602A publication Critical patent/JP2004529602A/ja
Publication of JP2004529602A5 publication Critical patent/JP2004529602A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
JP2001561782A 2000-02-22 2001-02-22 ミスマッチエンドヌクレアーゼをコードする核酸分子およびその使用方法 Expired - Fee Related JP4986358B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US09/510,322 US6391557B1 (en) 2000-02-22 2000-02-22 Nucleic acid molecule encoding a mismatch endonuclease and methods of use thereof
US09/510,322 2000-02-22
US09/514,768 2000-02-28
US09/514,768 US6699980B1 (en) 2000-02-28 2000-02-28 Nucleic acid molecule encoding a mismatch endonuclease and methods of use thereof
PCT/US2001/005502 WO2001062974A1 (en) 2000-02-22 2001-02-22 Nucleic acid molecule encoding a mismatch endonuclease and methods of use thereof

Publications (3)

Publication Number Publication Date
JP2004529602A JP2004529602A (ja) 2004-09-30
JP2004529602A5 JP2004529602A5 (enExample) 2008-04-10
JP4986358B2 true JP4986358B2 (ja) 2012-07-25

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JP2001561782A Expired - Fee Related JP4986358B2 (ja) 2000-02-22 2001-02-22 ミスマッチエンドヌクレアーゼをコードする核酸分子およびその使用方法

Country Status (5)

Country Link
EP (1) EP1257671B1 (enExample)
JP (1) JP4986358B2 (enExample)
AU (2) AU4721201A (enExample)
CA (1) CA2400441C (enExample)
WO (1) WO2001062974A1 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7838219B2 (en) 2001-02-02 2010-11-23 Novici Biotech Llc Method of increasing complementarity in a heteroduplex
WO2002079468A2 (en) 2001-02-02 2002-10-10 Large Scale Biology Corporation A method of increasing complementarity in a heteroduplex polynucleotide
US7582423B2 (en) 2001-02-02 2009-09-01 Novici Biotech Llc Population of polynucleotide sequence variants
US7078211B2 (en) 2002-02-01 2006-07-18 Large Scale Biology Corporation Nucleic acid molecules encoding endonucleases and methods of use thereof
DE10248258A1 (de) * 2002-10-16 2004-05-06 Biopsytec Analytik Gmbh Mutierte Nukleinsäure einer Cel I-Endonuklease und Verfahren zur Herstellung des rekombinanten vollständigen Cel I-Proteins
ATE495243T1 (de) * 2004-07-30 2011-01-15 Genoplante Valor Verfahren zur herstellung von hochempfindlichen endonukleasen, neuartige zubereitungen von nukleasen und ihre verwendung
CN101538561B (zh) * 2009-04-17 2010-09-29 广州大学 一种提取芹菜中celⅰ核酸酶的方法
KR102126744B1 (ko) 2013-03-14 2020-06-26 다카라 바이오 가부시키가이샤 내열성 미스매치 엔도뉴클레아제의 이용방법
KR20170053636A (ko) * 2014-09-11 2017-05-16 다카라 바이오 가부시키가이샤 내열성의 미스매치 엔도뉴클레아제의 이용방법
US11845928B2 (en) 2015-05-04 2023-12-19 Tsinghua University Methods and kits for fragmenting DNA
JP7734380B2 (ja) 2020-03-19 2025-09-05 タカラバイオ株式会社 耐熱性ミスマッチエンドヌクレアーゼ変異体
CN112301020B (zh) * 2020-10-19 2024-04-12 复旦大学附属肿瘤医院 Iii类脱氧核酶突变体及其制备方法与应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997046701A1 (en) * 1996-06-05 1997-12-11 Fox Chase Cancer Center Mismatch endonucleases and uses thereof in identifying mutations in targeted polynucleotide strands

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5869245A (en) * 1996-06-05 1999-02-09 Fox Chase Cancer Center Mismatch endonuclease and its use in identifying mutations in targeted polynucleotide strands

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997046701A1 (en) * 1996-06-05 1997-12-11 Fox Chase Cancer Center Mismatch endonucleases and uses thereof in identifying mutations in targeted polynucleotide strands

Also Published As

Publication number Publication date
EP1257671A1 (en) 2002-11-20
JP2004529602A (ja) 2004-09-30
CA2400441A1 (en) 2001-08-30
WO2001062974A1 (en) 2001-08-30
EP1257671B1 (en) 2015-01-14
EP1257671A4 (en) 2005-08-17
AU4721201A (en) 2001-09-03
CA2400441C (en) 2013-08-27
AU2001247212B2 (en) 2005-12-22

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