CA2400441C - Nucleic acid molecule encoding a mismatch endonuclease and methods of use thereof - Google Patents
Nucleic acid molecule encoding a mismatch endonuclease and methods of use thereof Download PDFInfo
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- CA2400441C CA2400441C CA2400441A CA2400441A CA2400441C CA 2400441 C CA2400441 C CA 2400441C CA 2400441 A CA2400441 A CA 2400441A CA 2400441 A CA2400441 A CA 2400441A CA 2400441 C CA2400441 C CA 2400441C
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- sequence
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- dna
- endonuclease
- nucleic acid
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- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 125000000430 tryptophan group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C2=C([H])C([H])=C([H])C([H])=C12 0.000 description 1
- 108010080629 tryptophan-leucine Proteins 0.000 description 1
- 108010038745 tryptophylglycine Proteins 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
- 108010027345 wheylin-1 peptide Proteins 0.000 description 1
- 238000002424 x-ray crystallography Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
- C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/510,322 US6391557B1 (en) | 2000-02-22 | 2000-02-22 | Nucleic acid molecule encoding a mismatch endonuclease and methods of use thereof |
| US09/510,322 | 2000-02-22 | ||
| US09/514,768 | 2000-02-28 | ||
| US09/514,768 US6699980B1 (en) | 2000-02-28 | 2000-02-28 | Nucleic acid molecule encoding a mismatch endonuclease and methods of use thereof |
| PCT/US2001/005502 WO2001062974A1 (en) | 2000-02-22 | 2001-02-22 | Nucleic acid molecule encoding a mismatch endonuclease and methods of use thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2400441A1 CA2400441A1 (en) | 2001-08-30 |
| CA2400441C true CA2400441C (en) | 2013-08-27 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2400441A Expired - Lifetime CA2400441C (en) | 2000-02-22 | 2001-02-22 | Nucleic acid molecule encoding a mismatch endonuclease and methods of use thereof |
Country Status (5)
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|---|---|
| EP (1) | EP1257671B1 (enExample) |
| JP (1) | JP4986358B2 (enExample) |
| AU (2) | AU4721201A (enExample) |
| CA (1) | CA2400441C (enExample) |
| WO (1) | WO2001062974A1 (enExample) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7838219B2 (en) | 2001-02-02 | 2010-11-23 | Novici Biotech Llc | Method of increasing complementarity in a heteroduplex |
| WO2002079468A2 (en) | 2001-02-02 | 2002-10-10 | Large Scale Biology Corporation | A method of increasing complementarity in a heteroduplex polynucleotide |
| US7582423B2 (en) | 2001-02-02 | 2009-09-01 | Novici Biotech Llc | Population of polynucleotide sequence variants |
| US7078211B2 (en) | 2002-02-01 | 2006-07-18 | Large Scale Biology Corporation | Nucleic acid molecules encoding endonucleases and methods of use thereof |
| DE10248258A1 (de) * | 2002-10-16 | 2004-05-06 | Biopsytec Analytik Gmbh | Mutierte Nukleinsäure einer Cel I-Endonuklease und Verfahren zur Herstellung des rekombinanten vollständigen Cel I-Proteins |
| ATE495243T1 (de) * | 2004-07-30 | 2011-01-15 | Genoplante Valor | Verfahren zur herstellung von hochempfindlichen endonukleasen, neuartige zubereitungen von nukleasen und ihre verwendung |
| CN101538561B (zh) * | 2009-04-17 | 2010-09-29 | 广州大学 | 一种提取芹菜中celⅰ核酸酶的方法 |
| KR102126744B1 (ko) | 2013-03-14 | 2020-06-26 | 다카라 바이오 가부시키가이샤 | 내열성 미스매치 엔도뉴클레아제의 이용방법 |
| KR20170053636A (ko) * | 2014-09-11 | 2017-05-16 | 다카라 바이오 가부시키가이샤 | 내열성의 미스매치 엔도뉴클레아제의 이용방법 |
| US11845928B2 (en) | 2015-05-04 | 2023-12-19 | Tsinghua University | Methods and kits for fragmenting DNA |
| JP7734380B2 (ja) | 2020-03-19 | 2025-09-05 | タカラバイオ株式会社 | 耐熱性ミスマッチエンドヌクレアーゼ変異体 |
| CN112301020B (zh) * | 2020-10-19 | 2024-04-12 | 复旦大学附属肿瘤医院 | Iii类脱氧核酶突变体及其制备方法与应用 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997046701A1 (en) * | 1996-06-05 | 1997-12-11 | Fox Chase Cancer Center | Mismatch endonucleases and uses thereof in identifying mutations in targeted polynucleotide strands |
| US5869245A (en) * | 1996-06-05 | 1999-02-09 | Fox Chase Cancer Center | Mismatch endonuclease and its use in identifying mutations in targeted polynucleotide strands |
-
2001
- 2001-02-22 AU AU4721201A patent/AU4721201A/xx active Pending
- 2001-02-22 WO PCT/US2001/005502 patent/WO2001062974A1/en not_active Ceased
- 2001-02-22 AU AU2001247212A patent/AU2001247212B2/en not_active Ceased
- 2001-02-22 EP EP01920126.8A patent/EP1257671B1/en not_active Expired - Lifetime
- 2001-02-22 JP JP2001561782A patent/JP4986358B2/ja not_active Expired - Fee Related
- 2001-02-22 CA CA2400441A patent/CA2400441C/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JP4986358B2 (ja) | 2012-07-25 |
| EP1257671A1 (en) | 2002-11-20 |
| JP2004529602A (ja) | 2004-09-30 |
| CA2400441A1 (en) | 2001-08-30 |
| WO2001062974A1 (en) | 2001-08-30 |
| EP1257671B1 (en) | 2015-01-14 |
| EP1257671A4 (en) | 2005-08-17 |
| AU4721201A (en) | 2001-09-03 |
| AU2001247212B2 (en) | 2005-12-22 |
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