JP4965434B2 - 蛍光共鳴エネルギー移動酵素基質 - Google Patents

蛍光共鳴エネルギー移動酵素基質 Download PDF

Info

Publication number
JP4965434B2
JP4965434B2 JP2007509542A JP2007509542A JP4965434B2 JP 4965434 B2 JP4965434 B2 JP 4965434B2 JP 2007509542 A JP2007509542 A JP 2007509542A JP 2007509542 A JP2007509542 A JP 2007509542A JP 4965434 B2 JP4965434 B2 JP 4965434B2
Authority
JP
Japan
Prior art keywords
dye
enzyme
dyes
group
compound
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2007509542A
Other languages
English (en)
Japanese (ja)
Other versions
JP2007533828A (ja
JP2007533828A5 (enExample
Inventor
クマール,シヴ
スード,アヌープ
Original Assignee
ジーイー・ヘルスケア・アクスイェ・セルスカプ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ジーイー・ヘルスケア・アクスイェ・セルスカプ filed Critical ジーイー・ヘルスケア・アクスイェ・セルスカプ
Publication of JP2007533828A publication Critical patent/JP2007533828A/ja
Publication of JP2007533828A5 publication Critical patent/JP2007533828A5/ja
Application granted granted Critical
Publication of JP4965434B2 publication Critical patent/JP4965434B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Detergent Compositions (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
JP2007509542A 2004-04-23 2005-04-18 蛍光共鳴エネルギー移動酵素基質 Expired - Fee Related JP4965434B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US56492404P 2004-04-23 2004-04-23
US60/564,924 2004-04-23
PCT/US2005/013141 WO2005108994A1 (en) 2004-04-23 2005-04-18 Fluorescence resonance energy transfer enzyme substrates

Publications (3)

Publication Number Publication Date
JP2007533828A JP2007533828A (ja) 2007-11-22
JP2007533828A5 JP2007533828A5 (enExample) 2008-05-29
JP4965434B2 true JP4965434B2 (ja) 2012-07-04

Family

ID=34966026

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2007509542A Expired - Fee Related JP4965434B2 (ja) 2004-04-23 2005-04-18 蛍光共鳴エネルギー移動酵素基質

Country Status (8)

Country Link
US (2) US20090162838A1 (enExample)
EP (1) EP1738171B1 (enExample)
JP (1) JP4965434B2 (enExample)
CN (1) CN1985171B (enExample)
AT (1) ATE428113T1 (enExample)
DE (1) DE602005013757D1 (enExample)
ES (1) ES2324721T3 (enExample)
WO (1) WO2005108994A1 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0421693D0 (en) 2004-09-30 2004-11-03 Amersham Biosciences Uk Ltd Method for measuring binding of a test compound to a G-protein coupled receptor
JP5541894B2 (ja) * 2009-09-25 2014-07-09 アイシン精機株式会社 色素およびその製造方法
IT1405070B1 (it) * 2010-10-14 2013-12-16 Univ Bologna Alma Mater Nanoparticelle di silice drogate con una molteplicita' di coloranti caratterizzate da trasferimento di energia ad elevata efficienza e capaci di stokes-shift modulabile
CN103476944B (zh) * 2011-03-25 2016-01-20 艾尔玛可科学(苏格兰)有限公司 荧光寿命表观遗传学测定
US9322051B2 (en) 2013-10-07 2016-04-26 General Electric Company Probing of biological samples
CN104655596A (zh) * 2013-11-18 2015-05-27 李捷 一种含红细胞血液样品的质量检测方法及检测试剂盒
CN104109176B (zh) * 2014-06-06 2016-07-06 浙江工业大学 一种化合物及其应用于碱性磷酸酶活性的荧光检测方法
CN106405111A (zh) * 2016-09-10 2017-02-15 天津大学 基于弹性蛋白酶荧光底物检测乳腺癌肿瘤标志物ca153的酶联免疫试剂盒制备方法
WO2018159810A1 (ja) * 2017-03-03 2018-09-07 国立大学法人東京大学 アルカリフォスファターゼ検出用蛍光プローブ及びその使用
AR112979A1 (es) * 2017-08-24 2020-01-15 Commw Scient Ind Res Org Sensor para detectar hidrólisis
CN116046735B (zh) * 2022-12-26 2025-08-22 南京大学 一种利用染料组成的传感器阵列检测全氟化合物的方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5795729A (en) * 1996-02-05 1998-08-18 Biometric Imaging, Inc. Reductive, energy-transfer fluorogenic probes
GB0002261D0 (en) * 2000-02-02 2000-03-22 Amersham Pharm Biotech Uk Ltd Fluorescent detection method & reagent
US20020168641A1 (en) * 2001-03-09 2002-11-14 Bruce Mortensen Fluorescein-cyanine 5 as a fluorescence resonance energy transfer pair

Also Published As

Publication number Publication date
ES2324721T3 (es) 2009-08-13
ATE428113T1 (de) 2009-04-15
EP1738171B1 (en) 2009-04-08
CN1985171B (zh) 2012-08-08
US20120208223A1 (en) 2012-08-16
CN1985171A (zh) 2007-06-20
JP2007533828A (ja) 2007-11-22
EP1738171A1 (en) 2007-01-03
DE602005013757D1 (de) 2009-05-20
WO2005108994A1 (en) 2005-11-17
US20090162838A1 (en) 2009-06-25

Similar Documents

Publication Publication Date Title
US20120208223A1 (en) Fluorescence resonance energy transfer enzyme substrates
Hewitt et al. Application of lanthanide luminescence in probing enzyme activity
EP1910554B1 (en) Cyanine dyes and methods of use in biological applications
JP6644713B2 (ja) キノンメチドアナログシグナル増幅
US8586325B2 (en) Enzyme substrate comprising a functional dye and associated technology and methods
Zhang et al. Construction of an alkaline phosphatase-specific two-photon probe and its imaging application in living cells and tissues
EP2972229B1 (en) New silicon and germanium dyes for use in genetic identity
Guo et al. Fabrication of a water-soluble near-infrared fluorescent probe for selective detection and imaging of dipeptidyl peptidase IV in biological systems
Li et al. Recent advances in fluorescence imaging of alkaline phosphatase
EP1497452B1 (en) Methods for measuring enzyme activity
JP2007526766A (ja) ルミネセンス発生及びルミネセンス非発生多重アッセイ
JP2017522296A (ja) 活性化可能な水溶性の分子プローブ、その合成のための中間体、および関連する検出方法
EP2812698B1 (en) Dual-acceptor time-resolved-fret
Casey et al. Interrogating protein phosphatases with chemical activity probes
Hammler et al. Fluorescently labelled ATP analogues for direct monitoring of ubiquitin activation
Zou et al. A non-peptide probe for detecting chymotrypsin activity based on protection–deprotection strategy in living systems
JP4615867B2 (ja) プロテインキナーゼ及びホスファターゼ活性の測定法
JP7628233B2 (ja) Enpp活性検出用蛍光プローブ
GB2398123A (en) Methods for measuring enzyme activity

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20080410

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20080410

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20101201

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20101207

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20110304

RD02 Notification of acceptance of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7422

Effective date: 20110304

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20110304

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20110311

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20110606

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20120228

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20120329

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20150406

Year of fee payment: 3

LAPS Cancellation because of no payment of annual fees