ES2324721T3 - Sustratos enzimaticos de transferencia de energia de resonancia de fluorescencia. - Google Patents

Sustratos enzimaticos de transferencia de energia de resonancia de fluorescencia. Download PDF

Info

Publication number
ES2324721T3
ES2324721T3 ES05736155T ES05736155T ES2324721T3 ES 2324721 T3 ES2324721 T3 ES 2324721T3 ES 05736155 T ES05736155 T ES 05736155T ES 05736155 T ES05736155 T ES 05736155T ES 2324721 T3 ES2324721 T3 ES 2324721T3
Authority
ES
Spain
Prior art keywords
dyes
dye
group
enzyme
substrate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES05736155T
Other languages
English (en)
Spanish (es)
Inventor
Shiv Kumar
Anup Sood
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GE Healthcare AS
Original Assignee
GE Healthcare AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GE Healthcare AS filed Critical GE Healthcare AS
Application granted granted Critical
Publication of ES2324721T3 publication Critical patent/ES2324721T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Detergent Compositions (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
ES05736155T 2004-04-23 2005-04-18 Sustratos enzimaticos de transferencia de energia de resonancia de fluorescencia. Expired - Lifetime ES2324721T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US56492404P 2004-04-23 2004-04-23
US564924P 2004-04-23

Publications (1)

Publication Number Publication Date
ES2324721T3 true ES2324721T3 (es) 2009-08-13

Family

ID=34966026

Family Applications (1)

Application Number Title Priority Date Filing Date
ES05736155T Expired - Lifetime ES2324721T3 (es) 2004-04-23 2005-04-18 Sustratos enzimaticos de transferencia de energia de resonancia de fluorescencia.

Country Status (8)

Country Link
US (2) US20090162838A1 (enExample)
EP (1) EP1738171B1 (enExample)
JP (1) JP4965434B2 (enExample)
CN (1) CN1985171B (enExample)
AT (1) ATE428113T1 (enExample)
DE (1) DE602005013757D1 (enExample)
ES (1) ES2324721T3 (enExample)
WO (1) WO2005108994A1 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0421693D0 (en) 2004-09-30 2004-11-03 Amersham Biosciences Uk Ltd Method for measuring binding of a test compound to a G-protein coupled receptor
JP5541894B2 (ja) * 2009-09-25 2014-07-09 アイシン精機株式会社 色素およびその製造方法
IT1405070B1 (it) * 2010-10-14 2013-12-16 Univ Bologna Alma Mater Nanoparticelle di silice drogate con una molteplicita' di coloranti caratterizzate da trasferimento di energia ad elevata efficienza e capaci di stokes-shift modulabile
CN103476944B (zh) * 2011-03-25 2016-01-20 艾尔玛可科学(苏格兰)有限公司 荧光寿命表观遗传学测定
US9322051B2 (en) 2013-10-07 2016-04-26 General Electric Company Probing of biological samples
CN104655596A (zh) * 2013-11-18 2015-05-27 李捷 一种含红细胞血液样品的质量检测方法及检测试剂盒
CN104109176B (zh) * 2014-06-06 2016-07-06 浙江工业大学 一种化合物及其应用于碱性磷酸酶活性的荧光检测方法
CN106405111A (zh) * 2016-09-10 2017-02-15 天津大学 基于弹性蛋白酶荧光底物检测乳腺癌肿瘤标志物ca153的酶联免疫试剂盒制备方法
WO2018159810A1 (ja) * 2017-03-03 2018-09-07 国立大学法人東京大学 アルカリフォスファターゼ検出用蛍光プローブ及びその使用
AR112979A1 (es) * 2017-08-24 2020-01-15 Commw Scient Ind Res Org Sensor para detectar hidrólisis
CN116046735B (zh) * 2022-12-26 2025-08-22 南京大学 一种利用染料组成的传感器阵列检测全氟化合物的方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5795729A (en) * 1996-02-05 1998-08-18 Biometric Imaging, Inc. Reductive, energy-transfer fluorogenic probes
GB0002261D0 (en) * 2000-02-02 2000-03-22 Amersham Pharm Biotech Uk Ltd Fluorescent detection method & reagent
US20020168641A1 (en) * 2001-03-09 2002-11-14 Bruce Mortensen Fluorescein-cyanine 5 as a fluorescence resonance energy transfer pair

Also Published As

Publication number Publication date
ATE428113T1 (de) 2009-04-15
EP1738171B1 (en) 2009-04-08
JP4965434B2 (ja) 2012-07-04
CN1985171B (zh) 2012-08-08
US20120208223A1 (en) 2012-08-16
CN1985171A (zh) 2007-06-20
JP2007533828A (ja) 2007-11-22
EP1738171A1 (en) 2007-01-03
DE602005013757D1 (de) 2009-05-20
WO2005108994A1 (en) 2005-11-17
US20090162838A1 (en) 2009-06-25

Similar Documents

Publication Publication Date Title
US20120208223A1 (en) Fluorescence resonance energy transfer enzyme substrates
Wen et al. A space‐dependent ‘enzyme‐substrate’type probe based on ‘carboxylesterase‐amide group’for ultrafast fluorescent imaging orthotopic hepatocellular carcinoma
US9670476B2 (en) Enzyme substrate comprising a functional dye and associated technology and methods
Luo et al. Recent Advances in the Development of Optical Imaging Probes for γ‐Glutamyltranspeptidase
US20100184098A1 (en) Methods for measuring enzyme activity
Urano Novel live imaging techniques of cellular functions and in vivo tumors based on precise design of small molecule-based ‘activatable’fluorescence probes
CN113512579B (zh) 一种检测尿嘧啶dna糖基化酶的荧光生物传感器及其检测方法和应用
Li et al. Recent advances in fluorescence imaging of alkaline phosphatase
WO2015067302A1 (en) Sensor molecules and uses thereof
Guo et al. Fabrication of a water-soluble near-infrared fluorescent probe for selective detection and imaging of dipeptidyl peptidase IV in biological systems
CN109438326A (zh) 一种用于检测羧酸酯酶的荧光探针及其制备方法与专用检测试剂盒
Casey et al. Interrogating protein phosphatases with chemical activity probes
Biswas et al. Dual colorimetric and fluorogenic probes for visualizing tyrosine phosphatase activity
Yang et al. Real-time fluorescent determination and biological imaging in living models via a butyrylcholinesterase-activated fluorescent probe
CN108893106A (zh) 检测高浓度γ-谷氨酰转肽酶的荧光探针及其制备方法
Sun et al. Coumarin-based turn-on fluorescence probe with a large Stokes shift for detection of endogenous neutrophil elastase in live cells and zebrafish
Zou et al. A non-peptide probe for detecting chymotrypsin activity based on protection–deprotection strategy in living systems
Huang et al. Discovery of non-substrate, environmentally sensitive turn-on fluorescent probes for imaging HDAC8 in tumor cells and tissue slices
McIntyre et al. Optical proteolytic beacons for in vivo detection of matrix metalloproteinase activity
Guo et al. Benzorhodol derived far-red/near-infrared fluorescent probes for selective and sensitive detection of butyrylcholinesterase activity in living cells and the non-alcoholic fatty liver of zebrafish
ES2197811B2 (es) Sintesis de derivados fluorescentes de antiobioticos beta-lactamicos.
ES2316076T3 (es) Metodos para la determinacion de la actividad fosforiltrans-ferasa empleando adenosina 5'-trifosfato (atp) -gamma-s.
윤혜영 Reaction-based probe for enzyme activity assay and bioimaging
GB2398123A (en) Methods for measuring enzyme activity
Ermert Development of ATP-based Signaling Molecules