JP4951170B2 - Dnaシークエンシング方法 - Google Patents
Dnaシークエンシング方法 Download PDFInfo
- Publication number
- JP4951170B2 JP4951170B2 JP2000594948A JP2000594948A JP4951170B2 JP 4951170 B2 JP4951170 B2 JP 4951170B2 JP 2000594948 A JP2000594948 A JP 2000594948A JP 2000594948 A JP2000594948 A JP 2000594948A JP 4951170 B2 JP4951170 B2 JP 4951170B2
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- primer
- sequencing
- dna
- binding protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9901475.5A GB9901475D0 (en) | 1999-01-22 | 1999-01-22 | A method of DNA sequencing |
| GB9901475.5 | 1999-01-22 | ||
| PCT/GB2000/000146 WO2000043540A1 (en) | 1999-01-22 | 2000-01-20 | A method of dna sequencing |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2002538777A JP2002538777A (ja) | 2002-11-19 |
| JP2002538777A5 JP2002538777A5 (https=) | 2005-12-22 |
| JP4951170B2 true JP4951170B2 (ja) | 2012-06-13 |
Family
ID=10846383
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000594948A Expired - Lifetime JP4951170B2 (ja) | 1999-01-22 | 2000-01-20 | Dnaシークエンシング方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US6828100B1 (https=) |
| EP (1) | EP1147217B1 (https=) |
| JP (1) | JP4951170B2 (https=) |
| AT (1) | ATE446380T1 (https=) |
| AU (1) | AU3064200A (https=) |
| DE (1) | DE60043182D1 (https=) |
| GB (1) | GB9901475D0 (https=) |
| WO (1) | WO2000043540A1 (https=) |
Families Citing this family (393)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7622294B2 (en) | 1997-03-14 | 2009-11-24 | Trustees Of Tufts College | Methods for detecting target analytes and enzymatic reactions |
| US20030027126A1 (en) | 1997-03-14 | 2003-02-06 | Walt David R. | Methods for detecting target analytes and enzymatic reactions |
| US7875440B2 (en) | 1998-05-01 | 2011-01-25 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| US6780591B2 (en) | 1998-05-01 | 2004-08-24 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| US20050244870A1 (en) * | 1999-04-20 | 2005-11-03 | Illumina, Inc. | Nucleic acid sequencing using microsphere arrays |
| US20030108867A1 (en) * | 1999-04-20 | 2003-06-12 | Chee Mark S | Nucleic acid sequencing using microsphere arrays |
| US20060275782A1 (en) | 1999-04-20 | 2006-12-07 | Illumina, Inc. | Detection of nucleic acid reactions on bead arrays |
| US6818395B1 (en) | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
| US7501245B2 (en) | 1999-06-28 | 2009-03-10 | Helicos Biosciences Corp. | Methods and apparatuses for analyzing polynucleotide sequences |
| US7211390B2 (en) | 1999-09-16 | 2007-05-01 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
| US7244559B2 (en) | 1999-09-16 | 2007-07-17 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
| US6770441B2 (en) | 2000-02-10 | 2004-08-03 | Illumina, Inc. | Array compositions and methods of making same |
| GB0021977D0 (en) | 2000-09-07 | 2000-10-25 | Pyrosequencing Ab | Method of sequencing DNA |
| GB0022069D0 (en) * | 2000-09-08 | 2000-10-25 | Pyrosequencing Ab | Method |
| AU2002227156A1 (en) | 2000-12-01 | 2002-06-11 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
| JP2004523243A (ja) | 2001-03-12 | 2004-08-05 | カリフォルニア インスティチュート オブ テクノロジー | 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置 |
| US6613523B2 (en) | 2001-06-29 | 2003-09-02 | Agilent Technologies, Inc. | Method of DNA sequencing using cleavable tags |
| US6956114B2 (en) | 2001-10-30 | 2005-10-18 | '454 Corporation | Sulfurylase-luciferase fusion proteins and thermostable sulfurylase |
| US6902921B2 (en) | 2001-10-30 | 2005-06-07 | 454 Corporation | Sulfurylase-luciferase fusion proteins and thermostable sulfurylase |
| GB2390055B (en) | 2002-03-22 | 2005-09-07 | Cypher Science Internat Ltd | A marking apparatus |
| US7595883B1 (en) | 2002-09-16 | 2009-09-29 | The Board Of Trustees Of The Leland Stanford Junior University | Biological analysis arrangement and approach therefor |
| DE60324819D1 (de) | 2002-09-17 | 2009-01-02 | Aisin Cosmos R & D Co Ltd | Verfahren zur amplifikation von nukleinsäure, kit zur amplifikation von nukleinsäure, verfahren zum nachweis von einzelnukleotid-polymorphismus und reagentienkit zum nachweis von einzelnukleotid-polymorphismus |
| US7329545B2 (en) | 2002-09-24 | 2008-02-12 | Duke University | Methods for sampling a liquid flow |
| US6911132B2 (en) | 2002-09-24 | 2005-06-28 | Duke University | Apparatus for manipulating droplets by electrowetting-based techniques |
| WO2004029294A1 (en) * | 2002-09-27 | 2004-04-08 | Biotage Ab | New sequencing method for sequencing rna molecules |
| US7575865B2 (en) | 2003-01-29 | 2009-08-18 | 454 Life Sciences Corporation | Methods of amplifying and sequencing nucleic acids |
| CA2513899C (en) | 2003-01-29 | 2013-03-26 | 454 Corporation | Methods of amplifying and sequencing nucleic acids |
| CN103396933B (zh) | 2003-02-26 | 2016-04-20 | 凯利达基因组股份有限公司 | 通过杂交进行的随机阵列dna分析 |
| WO2004094666A1 (en) | 2003-04-24 | 2004-11-04 | Dzieglewska, Hanna | Allele-specific mutation detection assay |
| JP2005110621A (ja) * | 2003-10-10 | 2005-04-28 | Aisin Seiki Co Ltd | 核酸増幅方法及び核酸増幅用試薬キット |
| EP1711621A4 (en) | 2003-10-28 | 2008-06-18 | Dynal Biotech Llc | PRIMERS, METHODS AND KITS FOR THE AMPLIFICATION OR DETECTION OF HUMAN LEUKOCYTE ANTIGEN ALLELES |
| US7169560B2 (en) | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
| US7759057B2 (en) * | 2004-02-04 | 2010-07-20 | Ut-Battelle, Llc | Method for analyzing microbial communities |
| WO2005080605A2 (en) | 2004-02-19 | 2005-09-01 | Helicos Biosciences Corporation | Methods and kits for analyzing polynucleotide sequences |
| US7622281B2 (en) * | 2004-05-20 | 2009-11-24 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and compositions for clonal amplification of nucleic acid |
| US7476734B2 (en) | 2005-12-06 | 2009-01-13 | Helicos Biosciences Corporation | Nucleotide analogs |
| US7635562B2 (en) | 2004-05-25 | 2009-12-22 | Helicos Biosciences Corporation | Methods and devices for nucleic acid sequence determination |
| JP5037343B2 (ja) * | 2004-06-10 | 2012-09-26 | ジーイー・ヘルスケア・バイオサイエンス・コーポレイション | 核酸分析方法 |
| US7692219B1 (en) | 2004-06-25 | 2010-04-06 | University Of Hawaii | Ultrasensitive biosensors |
| US7361465B2 (en) * | 2004-09-07 | 2008-04-22 | Applera Corporation | Methods and compositions for tailing and amplifying RNA |
| US7785785B2 (en) | 2004-11-12 | 2010-08-31 | The Board Of Trustees Of The Leland Stanford Junior University | Charge perturbation detection system for DNA and other molecules |
| US7220549B2 (en) | 2004-12-30 | 2007-05-22 | Helicos Biosciences Corporation | Stabilizing a nucleic acid for nucleic acid sequencing |
| DK1859330T3 (da) * | 2005-01-28 | 2012-10-15 | Univ Duke | Apparater og fremgangsmåder til håndtering af små dråber på et trykt kredsløbskort |
| US7482120B2 (en) | 2005-01-28 | 2009-01-27 | Helicos Biosciences Corporation | Methods and compositions for improving fidelity in a nucleic acid synthesis reaction |
| US20060228721A1 (en) | 2005-04-12 | 2006-10-12 | Leamon John H | Methods for determining sequence variants using ultra-deep sequencing |
| EP3257949A1 (en) | 2005-06-15 | 2017-12-20 | Complete Genomics Inc. | Nucleic acid analysis by random mixtures of non-overlapping fragments |
| EP1907584A2 (en) * | 2005-06-28 | 2008-04-09 | Agencourt Personal Genomics Corporation | Methods of producing and sequencing modified polynucleotides |
| US7666593B2 (en) | 2005-08-26 | 2010-02-23 | Helicos Biosciences Corporation | Single molecule sequencing of captured nucleic acids |
| JP4353958B2 (ja) * | 2005-09-15 | 2009-10-28 | 株式会社日立製作所 | Dna計測装置、及びdna計測方法 |
| CA2624896C (en) | 2005-10-07 | 2017-11-07 | Callida Genomics, Inc. | Self-assembled single molecule arrays and uses thereof |
| US8364417B2 (en) | 2007-02-15 | 2013-01-29 | 454 Life Sciences Corporation | System and method to correct out of phase errors in DNA sequencing data by use of a recursive algorithm |
| US8301394B2 (en) * | 2006-02-16 | 2012-10-30 | 454 Life Sciences Corporation | System and method for correcting primer extension errors in nucleic acid sequence data |
| CN101432439B (zh) | 2006-02-24 | 2013-07-24 | 考利达基因组股份有限公司 | Dna阵列上的高通量基因组测序 |
| SG170028A1 (en) | 2006-02-24 | 2011-04-29 | Callida Genomics Inc | High throughput genome sequencing on dna arrays |
| US7397546B2 (en) | 2006-03-08 | 2008-07-08 | Helicos Biosciences Corporation | Systems and methods for reducing detected intensity non-uniformity in a laser beam |
| US20140193807A1 (en) | 2006-04-18 | 2014-07-10 | Advanced Liquid Logic, Inc. | Bead manipulation techniques |
| US8637317B2 (en) * | 2006-04-18 | 2014-01-28 | Advanced Liquid Logic, Inc. | Method of washing beads |
| US8613889B2 (en) * | 2006-04-13 | 2013-12-24 | Advanced Liquid Logic, Inc. | Droplet-based washing |
| US8492168B2 (en) | 2006-04-18 | 2013-07-23 | Advanced Liquid Logic Inc. | Droplet-based affinity assays |
| US9476856B2 (en) | 2006-04-13 | 2016-10-25 | Advanced Liquid Logic, Inc. | Droplet-based affinity assays |
| US7439014B2 (en) | 2006-04-18 | 2008-10-21 | Advanced Liquid Logic, Inc. | Droplet-based surface modification and washing |
| US8980198B2 (en) | 2006-04-18 | 2015-03-17 | Advanced Liquid Logic, Inc. | Filler fluids for droplet operations |
| US8809068B2 (en) | 2006-04-18 | 2014-08-19 | Advanced Liquid Logic, Inc. | Manipulation of beads in droplets and methods for manipulating droplets |
| US7815871B2 (en) | 2006-04-18 | 2010-10-19 | Advanced Liquid Logic, Inc. | Droplet microactuator system |
| US7763471B2 (en) * | 2006-04-18 | 2010-07-27 | Advanced Liquid Logic, Inc. | Method of electrowetting droplet operations for protein crystallization |
| US8389297B2 (en) * | 2006-04-18 | 2013-03-05 | Duke University | Droplet-based affinity assay device and system |
| US8637324B2 (en) | 2006-04-18 | 2014-01-28 | Advanced Liquid Logic, Inc. | Bead incubation and washing on a droplet actuator |
| US8716015B2 (en) | 2006-04-18 | 2014-05-06 | Advanced Liquid Logic, Inc. | Manipulation of cells on a droplet actuator |
| US7816121B2 (en) | 2006-04-18 | 2010-10-19 | Advanced Liquid Logic, Inc. | Droplet actuation system and method |
| US10078078B2 (en) | 2006-04-18 | 2018-09-18 | Advanced Liquid Logic, Inc. | Bead incubation and washing on a droplet actuator |
| US8470606B2 (en) * | 2006-04-18 | 2013-06-25 | Duke University | Manipulation of beads in droplets and methods for splitting droplets |
| WO2007123908A2 (en) * | 2006-04-18 | 2007-11-01 | Advanced Liquid Logic, Inc. | Droplet-based multiwell operations |
| US7901947B2 (en) | 2006-04-18 | 2011-03-08 | Advanced Liquid Logic, Inc. | Droplet-based particle sorting |
| US7822510B2 (en) | 2006-05-09 | 2010-10-26 | Advanced Liquid Logic, Inc. | Systems, methods, and products for graphically illustrating and controlling a droplet actuator |
| US7939021B2 (en) | 2007-05-09 | 2011-05-10 | Advanced Liquid Logic, Inc. | Droplet actuator analyzer with cartridge |
| US8041463B2 (en) * | 2006-05-09 | 2011-10-18 | Advanced Liquid Logic, Inc. | Modular droplet actuator drive |
| US11001881B2 (en) | 2006-08-24 | 2021-05-11 | California Institute Of Technology | Methods for detecting analytes |
| US11525156B2 (en) | 2006-07-28 | 2022-12-13 | California Institute Of Technology | Multiplex Q-PCR arrays |
| US8048626B2 (en) | 2006-07-28 | 2011-11-01 | California Institute Of Technology | Multiplex Q-PCR arrays |
| US11560588B2 (en) | 2006-08-24 | 2023-01-24 | California Institute Of Technology | Multiplex Q-PCR arrays |
| US7910354B2 (en) | 2006-10-27 | 2011-03-22 | Complete Genomics, Inc. | Efficient arrays of amplified polynucleotides |
| US20090111706A1 (en) | 2006-11-09 | 2009-04-30 | Complete Genomics, Inc. | Selection of dna adaptor orientation by amplification |
| US8349167B2 (en) | 2006-12-14 | 2013-01-08 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
| US8262900B2 (en) | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
| US11339430B2 (en) | 2007-07-10 | 2022-05-24 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
| GB2457851B (en) | 2006-12-14 | 2011-01-05 | Ion Torrent Systems Inc | Methods and apparatus for measuring analytes using large scale fet arrays |
| US7932034B2 (en) | 2006-12-20 | 2011-04-26 | The Board Of Trustees Of The Leland Stanford Junior University | Heat and pH measurement for sequencing of DNA |
| US11940413B2 (en) | 2007-02-05 | 2024-03-26 | IsoPlexis Corporation | Methods and devices for sequencing nucleic acids in smaller batches |
| US8551704B2 (en) * | 2007-02-16 | 2013-10-08 | Pacific Biosciences Of California, Inc. | Controllable strand scission of mini circle DNA |
| JP2010528608A (ja) * | 2007-06-01 | 2010-08-26 | 454 ライフ サイエンシーズ コーポレイション | 複合的な混合物から個々の試料を特定するためのシステムおよび方法 |
| US20090053724A1 (en) * | 2007-06-28 | 2009-02-26 | 454 Life Sciences Corporation | System and method for adaptive reagent control in nucleic acid sequencing |
| US8268246B2 (en) | 2007-08-09 | 2012-09-18 | Advanced Liquid Logic Inc | PCB droplet actuator fabrication |
| US8951731B2 (en) | 2007-10-15 | 2015-02-10 | Complete Genomics, Inc. | Sequence analysis using decorated nucleic acids |
| US8415099B2 (en) | 2007-11-05 | 2013-04-09 | Complete Genomics, Inc. | Efficient base determination in sequencing reactions |
| US8298768B2 (en) | 2007-11-29 | 2012-10-30 | Complete Genomics, Inc. | Efficient shotgun sequencing methods |
| US7897344B2 (en) | 2007-11-06 | 2011-03-01 | Complete Genomics, Inc. | Methods and oligonucleotide designs for insertion of multiple adaptors into library constructs |
| US8518640B2 (en) | 2007-10-29 | 2013-08-27 | Complete Genomics, Inc. | Nucleic acid sequencing and process |
| WO2009061840A1 (en) | 2007-11-05 | 2009-05-14 | Complete Genomics, Inc. | Methods and oligonucleotide designs for insertion of multiple adaptors employing selective methylation |
| US9551026B2 (en) | 2007-12-03 | 2017-01-24 | Complete Genomincs, Inc. | Method for nucleic acid detection using voltage enhancement |
| EP2565279B1 (en) | 2007-12-05 | 2014-12-03 | Complete Genomics, Inc. | Efficient base determination in sequencing reactions |
| US8592150B2 (en) | 2007-12-05 | 2013-11-26 | Complete Genomics, Inc. | Methods and compositions for long fragment read sequencing |
| WO2009097368A2 (en) * | 2008-01-28 | 2009-08-06 | Complete Genomics, Inc. | Methods and compositions for efficient base calling in sequencing reactions |
| US20090203086A1 (en) * | 2008-02-06 | 2009-08-13 | 454 Life Sciences Corporation | System and method for improved signal detection in nucleic acid sequencing |
| US9017973B2 (en) | 2008-03-19 | 2015-04-28 | Intelligent Biosystems, Inc. | Methods and compositions for incorporating nucleotides |
| US8206905B2 (en) * | 2008-04-26 | 2012-06-26 | Valeri Golovlev | Enzymatic time-resolved luminescent assay for nucleic acids quantitation |
| EP2307577B1 (en) | 2008-06-25 | 2015-06-03 | Life Technologies Corporation | Methods for measuring analytes using large scale fet arrays |
| US7888034B2 (en) | 2008-07-01 | 2011-02-15 | 454 Life Sciences Corporation | System and method for detection of HIV tropism variants |
| WO2010003153A2 (en) * | 2008-07-03 | 2010-01-07 | Life Technologies Corporation | Methylation analysis of mate pairs |
| US20100261189A1 (en) * | 2008-10-03 | 2010-10-14 | Roche Molecular Systems, Inc. | System and method for detection of HLA Variants |
| US20100301398A1 (en) | 2009-05-29 | 2010-12-02 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
| US20100137143A1 (en) * | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
| EP2394165A4 (en) * | 2009-02-03 | 2013-12-11 | Complete Genomics Inc | ASSIGNMENT OF OLIGOMER SEQUENCES |
| US8738296B2 (en) * | 2009-02-03 | 2014-05-27 | Complete Genomics, Inc. | Indexing a reference sequence for oligomer sequence mapping |
| EP2394164A4 (en) * | 2009-02-03 | 2014-01-08 | Complete Genomics Inc | ASSIGNMENT OF OLIGOMER SEQUENCES |
| US20110020806A1 (en) * | 2009-03-31 | 2011-01-27 | The Regents Of The University Of California | Rapid DNA Sequencing by Peroxidative Reaction |
| EP2511843B1 (en) * | 2009-04-29 | 2016-12-21 | Complete Genomics, Inc. | Method and system for calling variations in a sample polynucleotide sequence with respect to a reference polynucleotide sequence |
| US12129514B2 (en) | 2009-04-30 | 2024-10-29 | Molecular Loop Biosolutions, Llc | Methods and compositions for evaluating genetic markers |
| EP2425240A4 (en) | 2009-04-30 | 2012-12-12 | Good Start Genetics Inc | METHOD AND COMPOSITION FOR EVALUATING GENETIC MARKERS |
| JP5618497B2 (ja) * | 2009-05-15 | 2014-11-05 | 株式会社日立ハイテクノロジーズ | 核酸分析方法及び核酸分析装置 |
| US8673627B2 (en) | 2009-05-29 | 2014-03-18 | Life Technologies Corporation | Apparatus and methods for performing electrochemical reactions |
| US8776573B2 (en) | 2009-05-29 | 2014-07-15 | Life Technologies Corporation | Methods and apparatus for measuring analytes |
| US20120261274A1 (en) | 2009-05-29 | 2012-10-18 | Life Technologies Corporation | Methods and apparatus for measuring analytes |
| US8574835B2 (en) | 2009-05-29 | 2013-11-05 | Life Technologies Corporation | Scaffolded nucleic acid polymer particles and methods of making and using |
| US9524369B2 (en) | 2009-06-15 | 2016-12-20 | Complete Genomics, Inc. | Processing and analysis of complex nucleic acid sequence data |
| EP2977455B1 (en) | 2009-06-15 | 2020-04-15 | Complete Genomics, Inc. | Method for long fragment read sequencing |
| US8609339B2 (en) | 2009-10-09 | 2013-12-17 | 454 Life Sciences Corporation | System and method for emulsion breaking and recovery of biological elements |
| BR112012009023A2 (pt) | 2009-10-22 | 2019-09-24 | Biotools Biotechnologial & Medical Laboratories S A | método pra detecção. diferenciação e identificação de eubactérias específica de táxons em uma amostra biológica por meio de técnicas de análise de sequenciamento e kit |
| US8574832B2 (en) | 2010-02-03 | 2013-11-05 | Massachusetts Institute Of Technology | Methods for preparing sequencing libraries |
| WO2011127099A1 (en) | 2010-04-05 | 2011-10-13 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
| US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| US9125931B2 (en) | 2010-04-06 | 2015-09-08 | Massachusetts Institute Of Technology | Post-transcriptional regulation of RNA-related processes using encoded protein-binding RNA aptamers |
| CN103168118A (zh) | 2010-04-06 | 2013-06-19 | 麻省理工学院 | 用减少数量的转录物测量进行的基因表达概况分析 |
| US8774494B2 (en) | 2010-04-30 | 2014-07-08 | Complete Genomics, Inc. | Method and system for accurate alignment and registration of array for DNA sequencing |
| CA2793970A1 (en) | 2010-04-30 | 2011-11-03 | F. Hoffmann-La Roche Ag | System and method for purification and use of inorganic pyrophosphatase from aquifex aeolicus |
| US20110287432A1 (en) | 2010-05-21 | 2011-11-24 | 454 Life Science Corporation | System and method for tailoring nucleotide concentration to enzymatic efficiencies in dna sequencing technologies |
| AU2011226792A1 (en) | 2010-06-11 | 2012-01-12 | Life Technologies Corporation | Alternative nucleotide flows in sequencing-by-synthesis methods |
| TWI465716B (zh) | 2010-06-30 | 2014-12-21 | 生命技術公司 | 用於檢測及測量化學反應及化合物之電晶體電路 |
| EP2588851B1 (en) | 2010-06-30 | 2016-12-21 | Life Technologies Corporation | Ion-sensing charge-accumulation circuit and method |
| EP2588850B1 (en) | 2010-06-30 | 2016-12-28 | Life Technologies Corporation | Method for dry testing isfet arrays |
| US11307166B2 (en) | 2010-07-01 | 2022-04-19 | Life Technologies Corporation | Column ADC |
| US8653567B2 (en) | 2010-07-03 | 2014-02-18 | Life Technologies Corporation | Chemically sensitive sensor with lightly doped drains |
| EP2407242A1 (en) | 2010-07-13 | 2012-01-18 | Dublin City University | Direct clone analysis and selection technology |
| US11031095B2 (en) | 2010-08-06 | 2021-06-08 | Ariosa Diagnostics, Inc. | Assay systems for determination of fetal copy number variation |
| US11203786B2 (en) | 2010-08-06 | 2021-12-21 | Ariosa Diagnostics, Inc. | Detection of target nucleic acids using hybridization |
| US20120034603A1 (en) | 2010-08-06 | 2012-02-09 | Tandem Diagnostics, Inc. | Ligation-based detection of genetic variants |
| US20130040375A1 (en) | 2011-08-08 | 2013-02-14 | Tandem Diagnotics, Inc. | Assay systems for genetic analysis |
| US20130261003A1 (en) | 2010-08-06 | 2013-10-03 | Ariosa Diagnostics, In. | Ligation-based detection of genetic variants |
| US8700338B2 (en) | 2011-01-25 | 2014-04-15 | Ariosa Diagnosis, Inc. | Risk calculation for evaluation of fetal aneuploidy |
| US10167508B2 (en) | 2010-08-06 | 2019-01-01 | Ariosa Diagnostics, Inc. | Detection of genetic abnormalities |
| US10533223B2 (en) | 2010-08-06 | 2020-01-14 | Ariosa Diagnostics, Inc. | Detection of target nucleic acids using hybridization |
| US20140342940A1 (en) | 2011-01-25 | 2014-11-20 | Ariosa Diagnostics, Inc. | Detection of Target Nucleic Acids using Hybridization |
| US9671344B2 (en) | 2010-08-31 | 2017-06-06 | Complete Genomics, Inc. | High-density biochemical array chips with asynchronous tracks for alignment correction by moiré averaging |
| US9880089B2 (en) | 2010-08-31 | 2018-01-30 | Complete Genomics, Inc. | High-density devices with synchronous tracks for quad-cell based alignment correction |
| WO2012036679A1 (en) | 2010-09-15 | 2012-03-22 | Life Technologies Corporation | Methods and apparatus for measuring analytes |
| US8685324B2 (en) | 2010-09-24 | 2014-04-01 | Life Technologies Corporation | Matched pair transistor circuits |
| US20120077716A1 (en) | 2010-09-29 | 2012-03-29 | 454 Life Sciences Corporation | System and method for producing functionally distinct nucleic acid library ends through use of deoxyinosine |
| US20120322665A1 (en) | 2010-10-08 | 2012-12-20 | 454 Life Sciences Corporation | System and method for detection of hiv-1 clades and recombinants of the reverse transcriptase and protease regions |
| US8725422B2 (en) | 2010-10-13 | 2014-05-13 | Complete Genomics, Inc. | Methods for estimating genome-wide copy number variations |
| US8666678B2 (en) | 2010-10-27 | 2014-03-04 | Life Technologies Corporation | Predictive model for use in sequencing-by-synthesis |
| US10273540B2 (en) | 2010-10-27 | 2019-04-30 | Life Technologies Corporation | Methods and apparatuses for estimating parameters in a predictive model for use in sequencing-by-synthesis |
| EP2652659B1 (en) | 2010-12-14 | 2020-04-15 | Life Technologies Corporation | Systems and methods for run-time sequencing run quality monitoring |
| US9163281B2 (en) | 2010-12-23 | 2015-10-20 | Good Start Genetics, Inc. | Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction |
| EP2659408B1 (en) | 2010-12-29 | 2019-03-27 | Life Technologies Corporation | Time-warped background signal for sequencing-by-synthesis operations |
| EP3582224B1 (en) | 2010-12-30 | 2026-04-29 | Life Technologies Corporation | Models for analyzing data from sequencing-by-synthesis operations |
| US20130060482A1 (en) | 2010-12-30 | 2013-03-07 | Life Technologies Corporation | Methods, systems, and computer readable media for making base calls in nucleic acid sequencing |
| US10241075B2 (en) | 2010-12-30 | 2019-03-26 | Life Technologies Corporation | Methods, systems, and computer readable media for nucleic acid sequencing |
| US8756020B2 (en) | 2011-01-25 | 2014-06-17 | Ariosa Diagnostics, Inc. | Enhanced risk probabilities using biomolecule estimations |
| US20120190020A1 (en) | 2011-01-25 | 2012-07-26 | Aria Diagnostics, Inc. | Detection of genetic abnormalities |
| US9994897B2 (en) | 2013-03-08 | 2018-06-12 | Ariosa Diagnostics, Inc. | Non-invasive fetal sex determination |
| US10131947B2 (en) | 2011-01-25 | 2018-11-20 | Ariosa Diagnostics, Inc. | Noninvasive detection of fetal aneuploidy in egg donor pregnancies |
| US11270781B2 (en) | 2011-01-25 | 2022-03-08 | Ariosa Diagnostics, Inc. | Statistical analysis for non-invasive sex chromosome aneuploidy determination |
| WO2012118745A1 (en) | 2011-02-28 | 2012-09-07 | Arnold Oliphant | Assay systems for detection of aneuploidy and sex determination |
| US20120244523A1 (en) | 2011-03-25 | 2012-09-27 | 454 Life Sciences Corporation | System and Method for Detection of HIV Integrase Variants |
| EP2694709B1 (en) | 2011-04-08 | 2016-09-14 | Prognosys Biosciences, Inc. | Peptide constructs and assay systems |
| CN103764845B (zh) | 2011-04-08 | 2016-02-17 | 生命科技股份有限公司 | 用于合成测序中的相保护试剂流排序 |
| GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| US9513253B2 (en) | 2011-07-11 | 2016-12-06 | Advanced Liquid Logic, Inc. | Droplet actuators and techniques for droplet-based enzymatic assays |
| US10704164B2 (en) | 2011-08-31 | 2020-07-07 | Life Technologies Corporation | Methods, systems, computer readable media, and kits for sample identification |
| US20150087537A1 (en) | 2011-08-31 | 2015-03-26 | Life Technologies Corporation | Methods, Systems, Computer Readable Media, and Kits for Sample Identification |
| US8712697B2 (en) | 2011-09-07 | 2014-04-29 | Ariosa Diagnostics, Inc. | Determination of copy number variations using binomial probability calculations |
| CA2852665A1 (en) | 2011-10-17 | 2013-04-25 | Good Start Genetics, Inc. | Analysis methods |
| US10837879B2 (en) | 2011-11-02 | 2020-11-17 | Complete Genomics, Inc. | Treatment for stabilizing nucleic acid arrays |
| US9970984B2 (en) | 2011-12-01 | 2018-05-15 | Life Technologies Corporation | Method and apparatus for identifying defects in a chemical sensor array |
| US8747748B2 (en) | 2012-01-19 | 2014-06-10 | Life Technologies Corporation | Chemical sensor with conductive cup-shaped sensor surface |
| US8821798B2 (en) | 2012-01-19 | 2014-09-02 | Life Technologies Corporation | Titanium nitride as sensing layer for microwell structure |
| US20130217023A1 (en) | 2012-02-22 | 2013-08-22 | 454 Life Sciences Corporation | System And Method For Generation And Use Of Compact Clonally Amplified Products |
| US9803239B2 (en) | 2012-03-29 | 2017-10-31 | Complete Genomics, Inc. | Flow cells for high density array chips |
| US8209130B1 (en) | 2012-04-04 | 2012-06-26 | Good Start Genetics, Inc. | Sequence assembly |
| US8812422B2 (en) | 2012-04-09 | 2014-08-19 | Good Start Genetics, Inc. | Variant database |
| US10227635B2 (en) | 2012-04-16 | 2019-03-12 | Molecular Loop Biosolutions, Llc | Capture reactions |
| WO2013166517A1 (en) | 2012-05-04 | 2013-11-07 | Complete Genomics, Inc. | Methods for determining absolute genome-wide copy number variations of complex tumors |
| US9646132B2 (en) | 2012-05-11 | 2017-05-09 | Life Technologies Corporation | Models for analyzing data from sequencing-by-synthesis operations |
| US10192024B2 (en) | 2012-05-18 | 2019-01-29 | 454 Life Sciences Corporation | System and method for generation and use of optimal nucleotide flow orders |
| EP3937179A1 (en) | 2012-05-21 | 2022-01-12 | Distributed Bio Inc | Epitope focusing by variable effective antigen surface concentration |
| US10289800B2 (en) | 2012-05-21 | 2019-05-14 | Ariosa Diagnostics, Inc. | Processes for calculating phased fetal genomic sequences |
| EP4148142B1 (en) | 2012-05-21 | 2025-08-13 | The Scripps Research Institute | Methods of sample preparation |
| US8786331B2 (en) | 2012-05-29 | 2014-07-22 | Life Technologies Corporation | System for reducing noise in a chemical sensor array |
| WO2013184905A1 (en) | 2012-06-06 | 2013-12-12 | Myriad Genetics, Inc. | Hereditary cancer genes |
| US9488823B2 (en) | 2012-06-07 | 2016-11-08 | Complete Genomics, Inc. | Techniques for scanned illumination |
| US9628676B2 (en) | 2012-06-07 | 2017-04-18 | Complete Genomics, Inc. | Imaging systems with movable scan mirrors |
| EP2875156A4 (en) | 2012-07-19 | 2016-02-24 | Ariosa Diagnostics Inc | SEQUENTIAL LIGATION-BASED MULTIPLEX DETECTION OF GENETIC VARIANTS |
| US10329608B2 (en) | 2012-10-10 | 2019-06-25 | Life Technologies Corporation | Methods, systems, and computer readable media for repeat sequencing |
| EP4592400A3 (en) | 2012-10-17 | 2025-10-29 | 10x Genomics Sweden AB | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| EP2917368A1 (en) | 2012-11-07 | 2015-09-16 | Good Start Genetics, Inc. | Methods and systems for identifying contamination in samples |
| US9080968B2 (en) | 2013-01-04 | 2015-07-14 | Life Technologies Corporation | Methods and systems for point of use removal of sacrificial material |
| US9841398B2 (en) | 2013-01-08 | 2017-12-12 | Life Technologies Corporation | Methods for manufacturing well structures for low-noise chemical sensors |
| US8962366B2 (en) | 2013-01-28 | 2015-02-24 | Life Technologies Corporation | Self-aligned well structures for low-noise chemical sensors |
| US8963216B2 (en) | 2013-03-13 | 2015-02-24 | Life Technologies Corporation | Chemical sensor with sidewall spacer sensor surface |
| US8841217B1 (en) | 2013-03-13 | 2014-09-23 | Life Technologies Corporation | Chemical sensor with protruded sensor surface |
| EP2971159B1 (en) | 2013-03-14 | 2019-05-08 | Molecular Loop Biosolutions, LLC | Methods for analyzing nucleic acids |
| US20140296080A1 (en) | 2013-03-14 | 2014-10-02 | Life Technologies Corporation | Methods, Systems, and Computer Readable Media for Evaluating Variant Likelihood |
| EP2972279B1 (en) | 2013-03-15 | 2021-10-06 | Life Technologies Corporation | Chemical sensors with consistent sensor surface areas |
| US9116117B2 (en) | 2013-03-15 | 2015-08-25 | Life Technologies Corporation | Chemical sensor with sidewall sensor surface |
| WO2014149779A1 (en) | 2013-03-15 | 2014-09-25 | Life Technologies Corporation | Chemical device with thin conductive element |
| US9835585B2 (en) | 2013-03-15 | 2017-12-05 | Life Technologies Corporation | Chemical sensor with protruded sensor surface |
| CN105264366B (zh) | 2013-03-15 | 2019-04-16 | 生命科技公司 | 具有一致传感器表面区域的化学传感器 |
| US20140336063A1 (en) | 2013-05-09 | 2014-11-13 | Life Technologies Corporation | Windowed Sequencing |
| WO2014197377A2 (en) | 2013-06-03 | 2014-12-11 | Good Start Genetics, Inc. | Methods and systems for storing sequence read data |
| US10458942B2 (en) | 2013-06-10 | 2019-10-29 | Life Technologies Corporation | Chemical sensor array having multiple sensors per well |
| WO2014200579A1 (en) | 2013-06-13 | 2014-12-18 | Ariosa Diagnostics, Inc. | Statistical analysis for non-invasive sex chromosome aneuploidy determination |
| CA2916662C (en) | 2013-06-25 | 2022-03-08 | Prognosys Biosciences, Inc. | Methods and systems for determining spatial patterns of biological targets in a sample |
| US9926597B2 (en) | 2013-07-26 | 2018-03-27 | Life Technologies Corporation | Control nucleic acid sequences for use in sequencing-by-synthesis and methods for designing the same |
| US9898575B2 (en) | 2013-08-21 | 2018-02-20 | Seven Bridges Genomics Inc. | Methods and systems for aligning sequences |
| US9116866B2 (en) | 2013-08-21 | 2015-08-25 | Seven Bridges Genomics Inc. | Methods and systems for detecting sequence variants |
| US10726942B2 (en) | 2013-08-23 | 2020-07-28 | Complete Genomics, Inc. | Long fragment de novo assembly using short reads |
| EP2840148B1 (en) | 2013-08-23 | 2019-04-03 | F. Hoffmann-La Roche AG | Methods for nucleic acid amplification |
| US10351899B2 (en) | 2013-09-25 | 2019-07-16 | Bio-ID Diagnostics Inc. | Methods for detecting nucleic acid fragments |
| CN105683980B (zh) | 2013-10-04 | 2018-08-24 | 生命科技股份有限公司 | 在使用终止化学物质的测序中建立分阶段效应模型的方法和系统 |
| US10851414B2 (en) | 2013-10-18 | 2020-12-01 | Good Start Genetics, Inc. | Methods for determining carrier status |
| KR20160062763A (ko) | 2013-10-18 | 2016-06-02 | 세븐 브릿지스 지노믹스 인크. | 유전자 샘플을 유전자형 결정하기 위한 방법 및 시스템 |
| US11049587B2 (en) | 2013-10-18 | 2021-06-29 | Seven Bridges Genomics Inc. | Methods and systems for aligning sequences in the presence of repeating elements |
| SG11201603039PA (en) | 2013-10-18 | 2016-05-30 | Seven Bridges Genomics Inc | Methods and systems for identifying disease-induced mutations |
| EP3058096A1 (en) | 2013-10-18 | 2016-08-24 | Good Start Genetics, Inc. | Methods for assessing a genomic region of a subject |
| WO2015058095A1 (en) | 2013-10-18 | 2015-04-23 | Seven Bridges Genomics Inc. | Methods and systems for quantifying sequence alignment |
| US9092402B2 (en) | 2013-10-21 | 2015-07-28 | Seven Bridges Genomics Inc. | Systems and methods for using paired-end data in directed acyclic structure |
| KR20160107237A (ko) | 2014-01-10 | 2016-09-13 | 세븐 브릿지스 지노믹스 인크. | 판독물 맵핑에서 알려진 대립 유전자의 사용을 위한 시스템 및 방법 |
| US9817944B2 (en) | 2014-02-11 | 2017-11-14 | Seven Bridges Genomics Inc. | Systems and methods for analyzing sequence data |
| US11053548B2 (en) | 2014-05-12 | 2021-07-06 | Good Start Genetics, Inc. | Methods for detecting aneuploidy |
| WO2016025818A1 (en) | 2014-08-15 | 2016-02-18 | Good Start Genetics, Inc. | Systems and methods for genetic analysis |
| US10450562B2 (en) | 2014-09-09 | 2019-10-22 | Igenomx International Genomics Corporation | Methods and compositions for rapid nucleic acid library preparation |
| WO2016040446A1 (en) | 2014-09-10 | 2016-03-17 | Good Start Genetics, Inc. | Methods for selectively suppressing non-target sequences |
| JP2017536087A (ja) | 2014-09-24 | 2017-12-07 | グッド スタート ジェネティクス, インコーポレイテッド | 遺伝子アッセイのロバストネスを増大させるためのプロセス制御 |
| WO2016060974A1 (en) | 2014-10-13 | 2016-04-21 | Life Technologies Corporation | Methods, systems, and computer-readable media for accelerated base calling |
| CA2964349C (en) | 2014-10-14 | 2023-03-21 | Seven Bridges Genomics Inc. | Systems and methods for smart tools in sequence pipelines |
| CA3002133A1 (en) | 2014-10-17 | 2016-04-21 | Good Start Genetics, Inc. | Pre-implantation genetic screening and aneuploidy detection |
| US10000799B2 (en) | 2014-11-04 | 2018-06-19 | Boreal Genomics, Inc. | Methods of sequencing with linked fragments |
| US10077472B2 (en) | 2014-12-18 | 2018-09-18 | Life Technologies Corporation | High data rate integrated circuit with power management |
| KR102593647B1 (ko) | 2014-12-18 | 2023-10-26 | 라이프 테크놀로지스 코포레이션 | 트랜스미터 구성을 갖춘 높은 데이터율 집적 회로 |
| WO2016100521A1 (en) | 2014-12-18 | 2016-06-23 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale fet arrays |
| WO2016112073A1 (en) | 2015-01-06 | 2016-07-14 | Good Start Genetics, Inc. | Screening for structural variants |
| JP6773687B2 (ja) | 2015-02-17 | 2020-10-21 | エムジーアイ テック カンパニー リミテッドMGI Tech Co., Ltd. | 制御された鎖置換を用いてのdna配列決定 |
| WO2016141294A1 (en) | 2015-03-05 | 2016-09-09 | Seven Bridges Genomics Inc. | Systems and methods for genomic pattern analysis |
| WO2016156529A1 (en) * | 2015-03-31 | 2016-10-06 | Qiagen Gmbh | Efficiency improving ligation methods |
| EP3901282B1 (en) | 2015-04-10 | 2023-06-28 | Spatial Transcriptomics AB | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| EP4220645A3 (en) | 2015-05-14 | 2023-11-08 | Life Technologies Corporation | Barcode sequences, and related systems and methods |
| US10275567B2 (en) | 2015-05-22 | 2019-04-30 | Seven Bridges Genomics Inc. | Systems and methods for haplotyping |
| EP3323070B1 (en) | 2015-07-14 | 2024-05-08 | Personal Genome Diagnostics Inc. | Neoantigen analysis |
| WO2017020023A2 (en) | 2015-07-29 | 2017-02-02 | Progenity, Inc. | Nucleic acids and methods for detecting chromosomal abnormalities |
| US10793895B2 (en) | 2015-08-24 | 2020-10-06 | Seven Bridges Genomics Inc. | Systems and methods for epigenetic analysis |
| US10724110B2 (en) | 2015-09-01 | 2020-07-28 | Seven Bridges Genomics Inc. | Systems and methods for analyzing viral nucleic acids |
| US10584380B2 (en) | 2015-09-01 | 2020-03-10 | Seven Bridges Genomics Inc. | Systems and methods for mitochondrial analysis |
| WO2017062970A1 (en) | 2015-10-10 | 2017-04-13 | Guardant Health, Inc. | Methods and applications of gene fusion detection in cell-free dna analysis |
| US11347704B2 (en) | 2015-10-16 | 2022-05-31 | Seven Bridges Genomics Inc. | Biological graph or sequence serialization |
| WO2017087560A1 (en) | 2015-11-16 | 2017-05-26 | Progenity, Inc. | Nucleic acids and methods for detecting methylation status |
| SG11201805119QA (en) | 2015-12-17 | 2018-07-30 | Guardant Health Inc | Methods to determine tumor gene copy number by analysis of cell-free dna |
| US20170199960A1 (en) | 2016-01-07 | 2017-07-13 | Seven Bridges Genomics Inc. | Systems and methods for adaptive local alignment for graph genomes |
| US10364468B2 (en) | 2016-01-13 | 2019-07-30 | Seven Bridges Genomics Inc. | Systems and methods for analyzing circulating tumor DNA |
| US10460829B2 (en) | 2016-01-26 | 2019-10-29 | Seven Bridges Genomics Inc. | Systems and methods for encoding genetic variation for a population |
| US10262102B2 (en) | 2016-02-24 | 2019-04-16 | Seven Bridges Genomics Inc. | Systems and methods for genotyping with graph reference |
| WO2017155858A1 (en) | 2016-03-07 | 2017-09-14 | Insilixa, Inc. | Nucleic acid sequence identification using solid-phase cyclic single base extension |
| WO2017168332A1 (en) | 2016-03-28 | 2017-10-05 | Boreal Genomics, Inc. | Linked duplex target capture |
| US10961573B2 (en) | 2016-03-28 | 2021-03-30 | Boreal Genomics, Inc. | Linked duplex target capture |
| US10619205B2 (en) | 2016-05-06 | 2020-04-14 | Life Technologies Corporation | Combinatorial barcode sequences, and related systems and methods |
| CN109476695A (zh) | 2016-06-27 | 2019-03-15 | 丹娜法伯癌症研究院 | 用于测定rna翻译速率的方法 |
| US10600499B2 (en) | 2016-07-13 | 2020-03-24 | Seven Bridges Genomics Inc. | Systems and methods for reconciling variants in sequence data relative to reference sequence data |
| US11250931B2 (en) | 2016-09-01 | 2022-02-15 | Seven Bridges Genomics Inc. | Systems and methods for detecting recombination |
| EP4361287A3 (en) | 2016-09-28 | 2024-08-07 | Life Technologies Corporation | Methods for sequencing nucleic acids using termination chemistry |
| ES2948117T3 (es) | 2016-11-03 | 2023-08-31 | Mgi Tech Co Ltd | Biosensor |
| JP2019534051A (ja) | 2016-11-15 | 2019-11-28 | パーソナル ゲノム ダイアグノスティクス インコーポレイテッド | 遺伝子型決定アッセイにおける一意でないバーコード |
| US11268137B2 (en) | 2016-12-09 | 2022-03-08 | Boreal Genomics, Inc. | Linked ligation |
| ES3026859T3 (en) | 2017-01-04 | 2025-06-12 | Mgi Tech Co Ltd | Stepwise sequencing by non-labeled reversible terminators or natural nucleotides |
| WO2018175341A1 (en) | 2017-03-20 | 2018-09-27 | Complete Genomics, Inc. | Biosensors for biological or chemical analysis and methods of manufacturing the same |
| CN111295733B (zh) | 2017-09-19 | 2024-01-05 | 深圳华大智造科技股份有限公司 | 晶片级测序流通池制造 |
| WO2019108807A1 (en) | 2017-12-01 | 2019-06-06 | Personal Genome Diagnositics Inc. | Process for microsatellite instability detection |
| US12098419B2 (en) | 2018-08-23 | 2024-09-24 | Ncan Genomics, Inc. | Linked target capture and ligation |
| US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
| JP7535998B2 (ja) | 2018-08-31 | 2024-08-19 | ガーダント ヘルス, インコーポレイテッド | マージされたリードおよびマージされないリードに基づいた遺伝的変異体の検出 |
| TWI853851B (zh) | 2018-11-15 | 2024-09-01 | 中國商深圳華大智造科技有限公司 | 微流體裝置與其製備方法 |
| CN118853850A (zh) | 2018-12-01 | 2024-10-29 | 深圳华大智造科技股份有限公司 | 改善生物传感器光收集效率的方法和结构 |
| WO2020123316A2 (en) | 2018-12-10 | 2020-06-18 | 10X Genomics, Inc. | Methods for determining a location of a biological analyte in a biological sample |
| EP4538389A3 (en) | 2019-01-03 | 2025-07-30 | Ncan Genomics, Inc. | Linked target capture |
| US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| EP3908823A4 (en) | 2019-01-10 | 2022-10-12 | Travera LLC | CALIBRATION OF A FUNCTIONAL BIOMARKER INSTRUMENT |
| US12362038B2 (en) | 2019-01-10 | 2025-07-15 | Travera, Inc. | Identifying cancer therapies |
| EP3934684A4 (en) | 2019-01-10 | 2022-11-16 | Travera LLC | MACHINE LEARNING IN FUNCTIONAL CANCER TESTS |
| US12560590B2 (en) | 2019-01-23 | 2026-02-24 | Massachusetts Institute Of Technology | Rapid analysis of live cells |
| WO2020180813A1 (en) | 2019-03-06 | 2020-09-10 | Qiagen Sciences, Llc | Compositions and methods for adaptor design and nucleic acid library construction for rolony-based sequencing |
| JP7687957B2 (ja) | 2019-03-14 | 2025-06-03 | インシリクサ, インコーポレイテッド | 時間ゲート蛍光ベースの検出のための方法およびシステム |
| EP3947718A4 (en) | 2019-04-02 | 2022-12-21 | Enumera Molecular, Inc. | METHODS, SYSTEMS AND COMPOSITIONS FOR COUNTING NUCLEIC ACID MOLECULES |
| WO2020243579A1 (en) | 2019-05-30 | 2020-12-03 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
| WO2021092433A2 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
| WO2021091611A1 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Spatially-tagged analyte capture agents for analyte multiplexing |
| ES2982420T3 (es) | 2019-12-23 | 2024-10-16 | 10X Genomics Inc | Métodos para el análisis espacial mediante el uso de la ligazón con plantilla de ARN |
| EP4081656A1 (en) | 2019-12-23 | 2022-11-02 | 10X Genomics, Inc. | Compositions and methods for using fixed biological samples in partition-based assays |
| CN115135984B (zh) | 2019-12-23 | 2025-12-23 | 10X基因组学有限公司 | 可逆固定试剂及其使用方法 |
| US12365942B2 (en) | 2020-01-13 | 2025-07-22 | 10X Genomics, Inc. | Methods of decreasing background on a spatial array |
| CN115698282A (zh) | 2020-01-13 | 2023-02-03 | 福路伦特生物科学公司 | 单细胞测序 |
| US12405264B2 (en) | 2020-01-17 | 2025-09-02 | 10X Genomics, Inc. | Electrophoretic system and method for analyte capture |
| US11702693B2 (en) | 2020-01-21 | 2023-07-18 | 10X Genomics, Inc. | Methods for printing cells and generating arrays of barcoded cells |
| US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
| US20210230681A1 (en) | 2020-01-24 | 2021-07-29 | 10X Genomics, Inc. | Methods for spatial analysis using proximity ligation |
| US11821035B1 (en) | 2020-01-29 | 2023-11-21 | 10X Genomics, Inc. | Compositions and methods of making gene expression libraries |
| US12076701B2 (en) | 2020-01-31 | 2024-09-03 | 10X Genomics, Inc. | Capturing oligonucleotides in spatial transcriptomics |
| US12110548B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Bi-directional in situ analysis |
| US12110541B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Methods for preparing high-resolution spatial arrays |
| US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
| US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
| WO2021158925A1 (en) | 2020-02-07 | 2021-08-12 | 10X Genomics, Inc. | Quantitative and automated permeabilization performance evaluation for spatial transcriptomics |
| US11835462B2 (en) | 2020-02-11 | 2023-12-05 | 10X Genomics, Inc. | Methods and compositions for partitioning a biological sample |
| US12399123B1 (en) | 2020-02-14 | 2025-08-26 | 10X Genomics, Inc. | Spatial targeting of analytes |
| US12281357B1 (en) | 2020-02-14 | 2025-04-22 | 10X Genomics, Inc. | In situ spatial barcoding |
| US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
| US11926863B1 (en) | 2020-02-27 | 2024-03-12 | 10X Genomics, Inc. | Solid state single cell method for analyzing fixed biological cells |
| US11768175B1 (en) | 2020-03-04 | 2023-09-26 | 10X Genomics, Inc. | Electrophoretic methods for spatial analysis |
| US11866782B2 (en) | 2020-03-16 | 2024-01-09 | Fluent Biosciences Inc. | Multi-omic analysis in monodisperse droplets |
| CN115427586B (zh) | 2020-03-18 | 2025-03-28 | 深圳华大智造科技股份有限公司 | 大规模平行测序中的恢复阶段 |
| WO2021195169A1 (en) | 2020-03-24 | 2021-09-30 | Fluent Biosciences Inc. | Viral detection using template emulsification |
| EP4139485B1 (en) | 2020-04-22 | 2023-09-06 | 10X Genomics, Inc. | Methods for spatial analysis using targeted rna depletion |
| US12416603B2 (en) | 2020-05-19 | 2025-09-16 | 10X Genomics, Inc. | Electrophoresis cassettes and instrumentation |
| EP4414459B1 (en) | 2020-05-22 | 2025-09-03 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
| EP4153776B1 (en) | 2020-05-22 | 2025-03-05 | 10X Genomics, Inc. | Spatial analysis to detect sequence variants |
| WO2021242834A1 (en) | 2020-05-26 | 2021-12-02 | 10X Genomics, Inc. | Method for resetting an array |
| EP4600376A3 (en) | 2020-06-02 | 2025-10-22 | 10X Genomics, Inc. | Spatial transcriptomics for antigen-receptors |
| AU2021283174A1 (en) | 2020-06-02 | 2023-01-05 | 10X Genomics, Inc. | Nucleic acid library methods |
| US12265079B1 (en) | 2020-06-02 | 2025-04-01 | 10X Genomics, Inc. | Systems and methods for detecting analytes from captured single biological particles |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| EP4421186B1 (en) | 2020-06-08 | 2025-08-13 | 10X Genomics, Inc. | Methods of determining a surgical margin and methods of use thereof |
| US12435363B1 (en) | 2020-06-10 | 2025-10-07 | 10X Genomics, Inc. | Materials and methods for spatial transcriptomics |
| EP4165207B1 (en) | 2020-06-10 | 2024-09-25 | 10X Genomics, Inc. | Methods for determining a location of an analyte in a biological sample |
| WO2021252747A1 (en) | 2020-06-10 | 2021-12-16 | 1Ox Genomics, Inc. | Fluid delivery methods |
| ES2994976T3 (en) | 2020-06-25 | 2025-02-05 | 10X Genomics Inc | Spatial analysis of dna methylation |
| CN111635932B (zh) * | 2020-06-30 | 2022-07-08 | 北京启衡星生物科技有限公司 | 一种核酸聚合酶活性检测方法的应用及试剂盒 |
| US11981960B1 (en) | 2020-07-06 | 2024-05-14 | 10X Genomics, Inc. | Spatial analysis utilizing degradable hydrogels |
| US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
| US12209280B1 (en) | 2020-07-06 | 2025-01-28 | 10X Genomics, Inc. | Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis |
| EP4182477A4 (en) | 2020-07-15 | 2024-09-11 | Fluent Biosciences Inc. | MULTI-LEVEL LIGATION OLIGOS |
| US20220042109A1 (en) | 2020-08-06 | 2022-02-10 | Agendia NV | Methods of assessing breast cancer using circulating hormone receptor transcripts |
| US20220042106A1 (en) | 2020-08-06 | 2022-02-10 | Agendia NV | Systems and methods of using cell-free nucleic acids to tailor cancer treatment |
| WO2022029488A1 (en) | 2020-08-06 | 2022-02-10 | Agenda Nv | Systems and methods of assessing breast cancer |
| US20220042107A1 (en) | 2020-08-06 | 2022-02-10 | Agendia NV | Systems and methods of scoring risk and residual disease from passenger mutations |
| US12553898B1 (en) | 2020-08-10 | 2026-02-17 | 10X Genomics, Inc. | Fluorescent hybridization of antibody-oligonucleotide for multiplexing and signal amplification |
| US11981958B1 (en) | 2020-08-20 | 2024-05-14 | 10X Genomics, Inc. | Methods for spatial analysis using DNA capture |
| WO2022061150A2 (en) | 2020-09-18 | 2022-03-24 | 10X Geonomics, Inc. | Sample handling apparatus and image registration methods |
| US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
| KR20260019673A (ko) | 2020-10-13 | 2026-02-10 | 바이오-라드 유럽 게엠베하 | 식품 품질을 특성화하고 식품 안전을 개선할 때 적용되는 표적 검출을 위한 시스템 및 방법 |
| KR20230088443A (ko) | 2020-10-19 | 2023-06-19 | 바이오 래드 래버러토리스 인코오포레이티드 | 핵산 증폭 검정을 위한 적용분야를 갖는 신속한 다중화된 샘플 프로세싱을 위한 시스템 및 방법 |
| ES3063619T3 (en) | 2020-11-03 | 2026-04-17 | Illumina Inc | Systems and methods for making sequencing libraries |
| US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
| EP4729631A2 (en) | 2020-12-21 | 2026-04-22 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
| EP4421491A3 (en) | 2021-02-19 | 2024-11-27 | 10X Genomics, Inc. | Method of using a modular assay support device |
| EP4305190A4 (en) | 2021-03-11 | 2025-03-26 | Iggenix, Inc. | METHODS AND SYSTEMS FOR PREDICTING AN ALLERGIC REACTION |
| AU2022238446A1 (en) | 2021-03-18 | 2023-09-07 | 10X Genomics, Inc. | Multiplex capture of gene and protein expression from a biological sample |
| EP4305196B1 (en) | 2021-04-14 | 2025-04-02 | 10X Genomics, Inc. | Methods of measuring mislocalization of an analyte |
| US12176070B2 (en) * | 2021-04-19 | 2024-12-24 | University Of Utah Research Foundation | Systems and methods for facilitating rapid genome sequence analysis |
| EP4320271B1 (en) | 2021-05-06 | 2025-03-19 | 10X Genomics, Inc. | Methods for increasing resolution of spatial analysis |
| EP4341398A4 (en) | 2021-05-18 | 2025-02-19 | Fluent Biosciences Inc. | PRE-TEMPLATE INSTANT PARTITIONS FOR SCREENING |
| CA3220457A1 (en) | 2021-05-18 | 2022-11-24 | Fluent Biosciences Inc. | Multi-omic analysis of extracellular vesicles in monodisperse droplets |
| CA3220477A1 (en) | 2021-05-18 | 2022-11-24 | Fluent Biosciences Inc. | Perturbed genomic expression in pretemplated instant partitions |
| WO2022256503A1 (en) | 2021-06-03 | 2022-12-08 | 10X Genomics, Inc. | Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis |
| EP4363611A1 (en) | 2021-06-30 | 2024-05-08 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for enrichment of nucleic acids using light-mediated cross-linking |
| US12553805B2 (en) | 2021-08-02 | 2026-02-17 | 10X Genomics, Inc. | Methods of preserving a biological sample |
| WO2023034489A1 (en) | 2021-09-01 | 2023-03-09 | 10X Genomics, Inc. | Methods, compositions, and kits for blocking a capture probe on a spatial array |
| WO2023086880A1 (en) | 2021-11-10 | 2023-05-19 | 10X Genomics, Inc. | Methods, compositions, and kits for determining the location of an analyte in a biological sample |
| WO2023102118A2 (en) | 2021-12-01 | 2023-06-08 | 10X Genomics, Inc. | Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis |
| WO2023122033A1 (en) | 2021-12-20 | 2023-06-29 | 10X Genomics, Inc. | Self-test for pathology/histology slide imaging device |
| US11639499B1 (en) | 2022-03-08 | 2023-05-02 | Watchmaker Genomics, Inc. | Reverse transcriptase variants |
| WO2024015962A1 (en) | 2022-07-15 | 2024-01-18 | Pacific Biosciences Of California, Inc. | Blocked asymmetric hairpin adaptors |
| US12534721B2 (en) | 2022-10-06 | 2026-01-27 | Illumina, Inc. | Quantitative detection and analysis of moleculesip |
| EP4482979B1 (en) | 2022-11-09 | 2025-10-01 | 10X Genomics, Inc. | Methods, compositions, and kits for determining the location of multiple analytes in a biological sample |
| US20250069687A1 (en) | 2023-02-13 | 2025-02-27 | Guardant Health, Inc. | Characterization of whole genome duplication in a genomic cohort of over 14000 cell free dna samples |
| CN121100381A (zh) | 2023-04-17 | 2025-12-09 | 私人基因诊断公司 | 通过全基因组测序的综合分析进行的癌症检测 |
| WO2025010160A1 (en) | 2023-07-06 | 2025-01-09 | Pacific Biosciences Of California, Inc. | Methods and compositions for stabilizing concatemers |
| WO2025019313A1 (en) | 2023-07-14 | 2025-01-23 | The Broad Institute, Inc. | Method for assembling gene libraries from a pool of oligonucleotides |
| AU2024294323A1 (en) | 2023-07-14 | 2026-01-29 | The Broad Institute, Inc. | Preparation of molecular libraries by linked adapters |
| US12157920B1 (en) | 2023-11-15 | 2024-12-03 | Droplet Biosciences, Inc. | Methods for diagnosing and assessing disease using drain fluid |
| WO2025178954A1 (en) | 2024-02-23 | 2025-08-28 | Illumina, Inc. | Methods for sequencing and counting nucleic acids |
| US20250279155A1 (en) | 2024-03-04 | 2025-09-04 | Omniseq, Inc. | Consensus-based classification technique to determine genetically inffered ancestry from comprehensive genomic profiling of tumor dna |
| WO2025250836A1 (en) | 2024-05-29 | 2025-12-04 | Omniseq, Inc. | Ai-based multi-omics data processing for detection of genomic instability |
| WO2025254714A1 (en) | 2024-06-03 | 2025-12-11 | Illumina, Inc. | System and method for assembling a polynucleotide sequence |
| WO2026059922A1 (en) | 2024-09-10 | 2026-03-19 | Illumina, Inc. | Molecular deduplication analysis methods |
| WO2026064275A1 (en) | 2024-09-20 | 2026-03-26 | Somalogic, Inc. | Methods for detection of proteins and nucleic acids |
Family Cites Families (10)
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| US5646019A (en) | 1989-10-24 | 1997-07-08 | Stratagene | Method for producing primed nucleic acid templates |
| US5605824A (en) | 1989-10-24 | 1997-02-25 | Stratagene | Composition for hybridizing nucleic acids using single-stranded nucleic acid binding protein |
| US5449603A (en) | 1989-10-24 | 1995-09-12 | Stratagene | Method for hybridizing nucleic acids using single-stranded nucleic acid binding protein |
| GB9210168D0 (en) | 1992-05-12 | 1992-06-24 | Cemu Bioteknik Ab | Method of sequencing dna |
| US5547843A (en) * | 1992-07-17 | 1996-08-20 | Associated Universities, Inc. | Method for promoting specific alignment of short oligonucleotides on nucleic acids |
| US6075905A (en) | 1996-07-17 | 2000-06-13 | Sarnoff Corporation | Method and apparatus for mosaic image construction |
| GB9620209D0 (en) | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
| GB9626815D0 (en) * | 1996-12-23 | 1997-02-12 | Cemu Bioteknik Ab | Method of sequencing DNA |
| AU7813698A (en) | 1997-06-06 | 1998-12-21 | Nexstar Pharmaceuticals, Inc. | Nucleic acid detection |
| US6335162B1 (en) * | 1998-03-13 | 2002-01-01 | Promega Corporation | Nucleic acid detection |
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- 2000-01-20 US US09/889,812 patent/US6828100B1/en not_active Expired - Lifetime
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| ATE446380T1 (de) | 2009-11-15 |
| GB9901475D0 (en) | 1999-03-17 |
| US6828100B1 (en) | 2004-12-07 |
| AU3064200A (en) | 2000-08-07 |
| DE60043182D1 (de) | 2009-12-03 |
| EP1147217A1 (en) | 2001-10-24 |
| EP1147217B1 (en) | 2009-10-21 |
| JP2002538777A (ja) | 2002-11-19 |
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